| General information | |||
| TC033928
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 996122..1011137 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033928 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_09268 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033928 RA
MDENCENSV YNLITNLCE TFSYDQTVV KKLRSTAYE ILLKKRSHN QTSQRHLKG PDPHCSLLA WQFELATKY NLVDHGLKL KECGEYIQR HYEENIIEF LLCLRNVPV EKDDSVDFE LPFDVKSLY KIPSTPESE CSALIVDIA KNCSSTDVW TEASKLTFS NRKNWEDFG HPEPDKEKP FLCELGELS HLWVENLPS GYFSCFLQD ESTVEPVVK TVSEFVKDV KYLLVGMAS YFFSYTDES DFFLVPNLT VKGLSSEAL KSYSEEFIF CGNCYKALH EISTPCSLT GEYKQDGYI FAELCQSIQ RYLEIYRTT IMSLSDTIG LLKLHERTN QLRKQLTTL ASICKLGPF AQSERMPHG VLLLNYLYQ TALSLTDKN VLLVLYAVL FPCCQVYFS RFLQQWLLD GTVNDPFEE FFIKPNLKY ISTRGRTYW TRSYNIRED IVPDFLADL RMDILNCGK SMNLLKLCL QSSTFCSYL MGKKPLIIS CCLTSEQLS LLEQNATSY YLEILSECG PRINLEHIV RRNKEQDPI FMNLIAKKR AATLARLEL ERKKVLEEE REKKLDELT ALKEQYDSA VQLKQERIA SEIEHEIKL IQFNLSIEN TRDRLIERE ANELIDYYN KLYEISDLR RKKIETHIE KLKTCLEEP SNLETNSSE ESFYSVDEP MKETESEEK FDESETQPE EPIHTSKSL DIINANSEP ALETFATAK KIKEKVLTE EMGILMPPL KKQITTICD NHKLTDAQR NKLKVMSSE FGIEMRKPT IRKSLTQWE INRNHVLGT SEVFMPVKL DNVNYLNRN FTNSDISEK KIKKSNSLS LDFAKCDIP SPMSVDTTP MSESTTSRF GLSVSRGDF ESLPTTAET QATDEGFLF EGVPESSYV PIKRNFDDS LQKKVFSKR VTREEASGL STNCLRLFL HESVSIPLA TQTKLLDNE LLKYFVEDL QYLKHLNSL RDYFFLQDG EFGRNITET LFEKLYDVH FPIELINCQ TLQSLVLGA LQMSSKFQE NSTCLSFKI NSLPKRFHL GDPDVLDCL SLTYKVKWP LNILLPVDT IEKYDEVFK FLLKLNRIS WVLKKILLE LKILAKVSG KKEIYLMMS PHYRRVHQC RHVMLHFIQ TLQNYVVGE VLQSSWEVF ERNLVTVTN LDTLYSTHT NYIKTILFL CLLNQKSAP LRKVIQKIF VVILKFYDH LRSRAWKCE NGTYIHPNF AKLESIFLN FEEFVLYLF KIGRKVAKS GYQPHLTQL LDMLDVNGY YSNKNYVQV T
- >TC033928 RA
atggatgaa aattgtgaa aatagtgtg tataatcta atcaccaac ttgtgtgaa acattttct tatgaccag acggtcgtt aagaaactt cgatcaacg gcctatgaa attctgttg aaaaagaga agccacaat caaacaagt cagagacat ttgaaaggt cctgatcct cattgtagt ttgttagct tggcaattt gaacttgcg acaaagtac aatttagtg gatcatggt ttaaaattg aaggaatgt ggagaatat attcaaagg cattacgaa gaaaatatt attgagttt ttgttatgt ttgcgaaat gttccggtt gaaaaagac gattctgtg gattttgag ctacctttt gatgttaaa agtttatac aaaataccc tcgacacct gaaagcgaa tgctcagct ttgattgta gacattgct aaaaactgc tcatcaaca gacgtgtgg actgaggcg tccaaatta acatttagc aaccgaaaa aactgggaa gattttggg catcctgag cctgataag gaaaaaccg tttttgtgt gaattaggg gaattgtct catttatgg gttgagaat ttaccttct ggttatttt tcttgcttc ttgcaagat gagagtact gttgaacca gttgttaaa acagtgagt gaatttgtg aaagatgtc aagtatttg ctagtgggc atggcctct tacttcttc agctatacc gacgaaagt gattttttt ttggttcca aacctaaca gttaaaggg ctatcatca gaagcttta aaatcgtac agcgaggag tttattttt tgtggaaac tgttacaaa gctttacat gaaatttca actccctgc tcacttacg ggggaatac aaacaggac ggttatatt ttcgctgag ctatgccag agcattcag cgttatttg gaaatttat cgtacgaca attatgagt ctatctgac acaattggt ttattaaaa ttgcacgaa cgcactaat caattgcgc aaacaatta actacttta gcttcgatt tgtaaattg ggacctttt gcacagtca gagaggatg ccacatgga gtgttactg ctgaattat ctataccag acagcttta agtctcact gataaaaat gttttattg gtgttgtac gcagttttg ttcccttgc tgtcaggtc tatttcagc aggtttctc caacagtgg cttttggac ggcactgtt aacgatccg tttgaagaa tttttcatt aaaccaaat cttaaatac atatcgaca cgaggcagg acgtactgg accaggagt tacaacatc cgtgaagat atcgttcca gattttcta gcggatttg cgaatggat attctaaac tgcggcaaa tccatgaat ttgctaaaa ttatgctta caatcgagc acattttgc tcgtatcta atgggaaag aaaccgctg attatatct tgttgttta acttcggaa caattatcc ttgttggag caaaacgcg acgagttat taccttgaa attttatcc gaatgtggt ccgaggatt aacctggaa catatcgtt aggaggaat aaagaacag gatccgatc tttatgaat ttaatcgcc aagaaacgt gctgcgacg ttggcgaga cttgaactc gaacgaaag aaagtctta gaagaagaa agggagaaa aagctcgat gaactgacc gcattaaaa gaacagtat gattccgct gttcagcta aaacaagaa agaattgca tcggaaata gaacacgag atcaaacta atccagttt aacttatct attgaaaat actcgcgat aggttaatc gagagagaa gctaatgag ttaatcgat tattacaac aaattgtac gaaatatcg gaccttcgg cgaaaaaaa attgaaacc cacattgag aaactgaaa acttgtttg gaagagcca agtaatttg gaaacaaac tcttcagaa gagtctttc tattccgtt gatgaacca atgaaagag acagaaagt gaagagaaa ttcgatgag tcggagaca caaccagaa gaacctatt cacacttca aaatctctc gatataatc aatgccaac agcgaacca gcccttgaa acatttgcg acagcaaaa aaaatcaaa gaaaaggtt ttaacagaa gaaatggga atcttaatg ccaccatta aaaaaacaa atcactaca atttgcgac aaccacaaa ttaaccgat gcacagagg aacaaactt aaagtgatg agctccgaa ttcggcatc gaaatgagg aaaccgacg atcagaaaa agtttaact caatgggaa atcaaccga aatcatgtt ctaggcact agcgaggtg tttatgccg gtaaaactt gacaacgtt aattatctg aatcggaat tttacgaat agcgacata agtgagaaa aaaattaaa aaatcgaat agtctttct ttggatttt gccaaatgt gatatccct tcaccaatg agtgtcgac acgacccca atgagcgaa tcgacaacg agcagattc ggcctaagt gtgtcacga ggcgatttt gagagtctt cccacaact gctgaaact caagccact gacgaaggt ttcctgttt gaaggcgtc ccagaatcg agctatgtc ccaatcaag cgaaatttt gacgattcg ttgcagaaa aaagtcttc tcgaagcga gtaacacgc gaggaagcg tctggtctt agcacaaat tgtctgagg ctgtttctt cacgaaagt gtttcgatt ccgttagca acgcagaca aaactactc gataacgaa ttactcaaa tactttgtg gaggatttg caatacttg aagcatttg aatagtctt cgcgattat tttttcctg caagatggc gagtttggg cgcaacatt actgagact ctgttcgag aagttatac gatgtgcat tttcccatc gaactgatc aattgtcaa acgttgcag agtttggtt ttgggagcg ttgcagatg tcaagtaaa tttcaggaa aattcaact tgtttgtct ttcaagatc aatagtctg ccgaagagg tttcattta ggggatcct gatgttttg gattgcttg tcgctgact tataaagtt aaatggcct ttgaatatt ttgctgccg gtggacacg attgagaag tacgatgaa gtgttcaag tttttgctt aaacttaat cggatttcg tgggtgttg aagaaaatt cttttggaa ctgaagatc ttggcgaaa gtctcggga aagaaagag atttatttg atgatgtcg cctcattat cggcgagtg caccaatgc aggcacgtc atgttgcac tttatccaa acattgcaa aactatgtg gttggagag gtgctgcaa tcgagttgg gaagttttc gaacgcaat ttggtcaca gttacaaat ttggatact ttatattcg actcacacc aactacatt aaaacaatt ttattcctc tgtctgctt aatcaaaaa tcggcgcct ttgaggaaa gttattcaa aaaatattc gttgtcatt ttaaaattc tacgatcat ttgaggtca agggcgtgg aaatgcgag aatggaact tacattcat cccaatttt gcgaaattg gagagcatt tttcttaat tttgaagaa tttgttttg tacttgttt aagatagga cggaaggtt gcaaaaagt ggctaccag ccacattta acacagttg cttgatatg ttggatgtt aatggttat tattccaac aaaaattat gttcaagtt acttaa
- >TC033928 RA
cagttgtca tttgggtta agtcaaatt tggttaaat ttaaaaacg atggatgaa aattgtgaa aatagtgtg tataatcta atcaccaac ttgtgtgaa acattttct tatgaccag acggtcgtt aagaaactt cgatcaacg gcctatgaa attctgttg aaaaagaga agccacaat caaacaagt cagagacat ttgaaaggt cctgatcct cattgtagt ttgttagct tggcaattt gaacttgcg acaaagtac aatttagtg gatcatggt ttaaaattg aaggaatgt ggagaatat attcaaagg cattacgaa gaaaatatt attgagttt ttgttatgt ttgcgaaat gttccggtt gaaaaagac gattctgtg gattttgag ctacctttt gatgttaaa agtttatac aaaataccc tcgacacct gaaagcgaa tgctcagct ttgattgta gacattgct aaaaactgc tcatcaaca gacgtgtgg actgaggcg tccaaatta acatttagc aaccgaaaa aactgggaa gattttggg catcctgag cctgataag gaaaaaccg tttttgtgt gaattaggg gaattgtct catttatgg gttgagaat ttaccttct ggttatttt tcttgcttc ttgcaagat gagagtact gttgaacca gttgttaaa acagtgagt gaatttgtg aaagatgtc aagtatttg ctagtgggc atggcctct tacttcttc agctatacc gacgaaagt gattttttt ttggttcca aacctaaca gttaaaggg ctatcatca gaagcttta aaatcgtac agcgaggag tttattttt tgtggaaac tgttacaaa gctttacat gaaatttca actccctgc tcacttacg ggggaatac aaacaggac ggttatatt ttcgctgag ctatgccag agcattcag cgttatttg gaaatttat cgtacgaca attatgagt ctatctgac acaattggt ttattaaaa ttgcacgaa cgcactaat caattgcgc aaacaatta actacttta gcttcgatt tgtaaattg ggacctttt gcacagtca gagaggatg ccacatgga gtgttactg ctgaattat ctataccag acagcttta agtctcact gataaaaat gttttattg gtgttgtac gcagttttg ttcccttgc tgtcaggtc tatttcagc aggtttctc caacagtgg cttttggac ggcactgtt aacgatccg tttgaagaa tttttcatt aaaccaaat cttaaatac atatcgaca cgaggcagg acgtactgg accaggagt tacaacatc cgtgaagat atcgttcca gattttcta gcggatttg cgaatggat attctaaac tgcggcaaa tccatgaat ttgctaaaa ttatgctta caatcgagc acattttgc tcgtatcta atgggaaag aaaccgctg attatatct tgttgttta acttcggaa caattatcc ttgttggag caaaacgcg acgagttat taccttgaa attttatcc gaatgtggt ccgaggatt aacctggaa catatcgtt aggaggaat aaagaacag gatccgatc tttatgaat ttaatcgcc aagaaacgt gctgcgacg ttggcgaga cttgaactc gaacgaaag aaagtctta gaagaagaa agggagaaa aagctcgat gaactgacc gcattaaaa gaacagtat gattccgct gttcagcta aaacaagaa agaattgca tcggaaata gaacacgag atcaaacta atccagttt aacttatct attgaaaat actcgcgat aggttaatc gagagagaa gctaatgag ttaatcgat tattacaac aaattgtac gaaatatcg gaccttcgg cgaaaaaaa attgaaacc cacattgag aaactgaaa acttgtttg gaagagcca agtaatttg gaaacaaac tcttcagaa gagtctttc tattccgtt gatgaacca atgaaagag acagaaagt gaagagaaa ttcgatgag tcggagaca caaccagaa gaacctatt cacacttca aaatctctc gatataatc aatgccaac agcgaacca gcccttgaa acatttgcg acagcaaaa aaaatcaaa gaaaaggtt ttaacagaa gaaatggga atcttaatg ccaccatta aaaaaacaa atcactaca atttgcgac aaccacaaa ttaaccgat gcacagagg aacaaactt aaagtgatg agctccgaa ttcggcatc gaaatgagg aaaccgacg atcagaaaa agtttaact caatgggaa atcaaccga aatcatgtt ctaggcact agcgaggtg tttatgccg gtaaaactt gacaacgtt aattatctg aatcggaat tttacgaat agcgacata agtgagaaa aaaattaaa aaatcgaat agtctttct ttggatttt gccaaatgt gatatccct tcaccaatg agtgtcgac acgacccca atgagcgaa tcgacaacg agcagattc ggcctaagt gtgtcacga ggcgatttt gagagtctt cccacaact gctgaaact caagccact gacgaaggt ttcctgttt gaaggcgtc ccagaatcg agctatgtc ccaatcaag cgaaatttt gacgattcg ttgcagaaa aaagtcttc tcgaagcga gtaacacgc gaggaagcg tctggtctt agcacaaat tgtctgagg ctgtttctt cacgaaagt gtttcgatt ccgttagca acgcagaca aaactactc gataacgaa ttactcaaa tactttgtg gaggatttg caatacttg aagcatttg aatagtctt cgcgattat tttttcctg caagatggc gagtttggg cgcaacatt actgagact ctgttcgag aagttatac gatgtgcat tttcccatc gaactgatc aattgtcaa acgttgcag agtttggtt ttgggagcg ttgcagatg tcaagtaaa tttcaggaa aattcaact tgtttgtct ttcaagatc aatagtctg ccgaagagg tttcattta ggggatcct gatgttttg gattgcttg tcgctgact tataaagtt aaatggcct ttgaatatt ttgctgccg gtggacacg attgagaag tacgatgaa gtgttcaag tttttgctt aaacttaat cggatttcg tgggtgttg aagaaaatt cttttggaa ctgaagatc ttggcgaaa gtctcggga aagaaagag atttatttg atgatgtcg cctcattat cggcgagtg caccaatgc aggcacgtc atgttgcac tttatccaa acattgcaa aactatgtg gttggagag gtgctgcaa tcgagttgg gaagttttc gaacgcaat ttggtcaca gttacaaat ttggatact ttatattcg actcacacc aactacatt aaaacaatt ttattcctc tgtctgctt aatcaaaaa tcggcgcct ttgaggaaa gttattcaa aaaatattc gttgtcatt ttaaaattc tacgatcat ttgaggtca agggcgtgg aaatgcgag aatggaact tacattcat cccaatttt gcgaaattg gagagcatt tttcttaat tttgaagaa tttgttttg tacttgttt aagatagga cggaaggtt gcaaaaagt ggctaccag ccacattta acacagttg cttgatatg ttggatgtt aatggttat tattccaac aaaaattat gttcaagtt acttaaatt ttatcaagg ttgaataaa tgcttaaaa ctgat
