Status
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Gene information
General information
TC033928
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002175
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 996122..1011137
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033928
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09268

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033928 RA
      MDENCENSV YNLITNLCE TFSYDQTVV KKLRSTAYE 
      ILLKKRSHN QTSQRHLKG PDPHCSLLA WQFELATKY 
      NLVDHGLKL KECGEYIQR HYEENIIEF LLCLRNVPV 
      EKDDSVDFE LPFDVKSLY KIPSTPESE CSALIVDIA 
      KNCSSTDVW TEASKLTFS NRKNWEDFG HPEPDKEKP 
      FLCELGELS HLWVENLPS GYFSCFLQD ESTVEPVVK 
      TVSEFVKDV KYLLVGMAS YFFSYTDES DFFLVPNLT 
      VKGLSSEAL KSYSEEFIF CGNCYKALH EISTPCSLT 
      GEYKQDGYI FAELCQSIQ RYLEIYRTT IMSLSDTIG 
      LLKLHERTN QLRKQLTTL ASICKLGPF AQSERMPHG 
      VLLLNYLYQ TALSLTDKN VLLVLYAVL FPCCQVYFS 
      RFLQQWLLD GTVNDPFEE FFIKPNLKY ISTRGRTYW 
      TRSYNIRED IVPDFLADL RMDILNCGK SMNLLKLCL 
      QSSTFCSYL MGKKPLIIS CCLTSEQLS LLEQNATSY 
      YLEILSECG PRINLEHIV RRNKEQDPI FMNLIAKKR 
      AATLARLEL ERKKVLEEE REKKLDELT ALKEQYDSA 
      VQLKQERIA SEIEHEIKL IQFNLSIEN TRDRLIERE 
      ANELIDYYN KLYEISDLR RKKIETHIE KLKTCLEEP 
      SNLETNSSE ESFYSVDEP MKETESEEK FDESETQPE 
      EPIHTSKSL DIINANSEP ALETFATAK KIKEKVLTE 
      EMGILMPPL KKQITTICD NHKLTDAQR NKLKVMSSE 
      FGIEMRKPT IRKSLTQWE INRNHVLGT SEVFMPVKL 
      DNVNYLNRN FTNSDISEK KIKKSNSLS LDFAKCDIP 
      SPMSVDTTP MSESTTSRF GLSVSRGDF ESLPTTAET 
      QATDEGFLF EGVPESSYV PIKRNFDDS LQKKVFSKR 
      VTREEASGL STNCLRLFL HESVSIPLA TQTKLLDNE 
      LLKYFVEDL QYLKHLNSL RDYFFLQDG EFGRNITET 
      LFEKLYDVH FPIELINCQ TLQSLVLGA LQMSSKFQE 
      NSTCLSFKI NSLPKRFHL GDPDVLDCL SLTYKVKWP 
      LNILLPVDT IEKYDEVFK FLLKLNRIS WVLKKILLE 
      LKILAKVSG KKEIYLMMS PHYRRVHQC RHVMLHFIQ 
      TLQNYVVGE VLQSSWEVF ERNLVTVTN LDTLYSTHT 
      NYIKTILFL CLLNQKSAP LRKVIQKIF VVILKFYDH 
      LRSRAWKCE NGTYIHPNF AKLESIFLN FEEFVLYLF 
      KIGRKVAKS GYQPHLTQL LDMLDVNGY YSNKNYVQV 
      T
    CDS sequence
    • >TC033928 RA
      atggatgaa aattgtgaa aatagtgtg tataatcta 
      atcaccaac ttgtgtgaa acattttct tatgaccag 
      acggtcgtt aagaaactt cgatcaacg gcctatgaa 
      attctgttg aaaaagaga agccacaat caaacaagt 
      cagagacat ttgaaaggt cctgatcct cattgtagt 
      ttgttagct tggcaattt gaacttgcg acaaagtac 
      aatttagtg gatcatggt ttaaaattg aaggaatgt 
      ggagaatat attcaaagg cattacgaa gaaaatatt 
      attgagttt ttgttatgt ttgcgaaat gttccggtt 
      gaaaaagac gattctgtg gattttgag ctacctttt 
      gatgttaaa agtttatac aaaataccc tcgacacct 
      gaaagcgaa tgctcagct ttgattgta gacattgct 
      aaaaactgc tcatcaaca gacgtgtgg actgaggcg 
      tccaaatta acatttagc aaccgaaaa aactgggaa 
      gattttggg catcctgag cctgataag gaaaaaccg 
      tttttgtgt gaattaggg gaattgtct catttatgg 
      gttgagaat ttaccttct ggttatttt tcttgcttc 
      ttgcaagat gagagtact gttgaacca gttgttaaa 
      acagtgagt gaatttgtg aaagatgtc aagtatttg 
      ctagtgggc atggcctct tacttcttc agctatacc 
      gacgaaagt gattttttt ttggttcca aacctaaca 
      gttaaaggg ctatcatca gaagcttta aaatcgtac 
      agcgaggag tttattttt tgtggaaac tgttacaaa 
      gctttacat gaaatttca actccctgc tcacttacg 
      ggggaatac aaacaggac ggttatatt ttcgctgag 
      ctatgccag agcattcag cgttatttg gaaatttat 
      cgtacgaca attatgagt ctatctgac acaattggt 
      ttattaaaa ttgcacgaa cgcactaat caattgcgc 
      aaacaatta actacttta gcttcgatt tgtaaattg 
      ggacctttt gcacagtca gagaggatg ccacatgga 
      gtgttactg ctgaattat ctataccag acagcttta 
      agtctcact gataaaaat gttttattg gtgttgtac 
      gcagttttg ttcccttgc tgtcaggtc tatttcagc 
      aggtttctc caacagtgg cttttggac ggcactgtt 
      aacgatccg tttgaagaa tttttcatt aaaccaaat 
      cttaaatac atatcgaca cgaggcagg acgtactgg 
      accaggagt tacaacatc cgtgaagat atcgttcca 
      gattttcta gcggatttg cgaatggat attctaaac 
      tgcggcaaa tccatgaat ttgctaaaa ttatgctta 
      caatcgagc acattttgc tcgtatcta atgggaaag 
      aaaccgctg attatatct tgttgttta acttcggaa 
      caattatcc ttgttggag caaaacgcg acgagttat 
      taccttgaa attttatcc gaatgtggt ccgaggatt 
      aacctggaa catatcgtt aggaggaat aaagaacag 
      gatccgatc tttatgaat ttaatcgcc aagaaacgt 
      gctgcgacg ttggcgaga cttgaactc gaacgaaag 
      aaagtctta gaagaagaa agggagaaa aagctcgat 
      gaactgacc gcattaaaa gaacagtat gattccgct 
      gttcagcta aaacaagaa agaattgca tcggaaata 
      gaacacgag atcaaacta atccagttt aacttatct 
      attgaaaat actcgcgat aggttaatc gagagagaa 
      gctaatgag ttaatcgat tattacaac aaattgtac 
      gaaatatcg gaccttcgg cgaaaaaaa attgaaacc 
      cacattgag aaactgaaa acttgtttg gaagagcca 
      agtaatttg gaaacaaac tcttcagaa gagtctttc 
      tattccgtt gatgaacca atgaaagag acagaaagt 
      gaagagaaa ttcgatgag tcggagaca caaccagaa 
      gaacctatt cacacttca aaatctctc gatataatc 
      aatgccaac agcgaacca gcccttgaa acatttgcg 
      acagcaaaa aaaatcaaa gaaaaggtt ttaacagaa 
      gaaatggga atcttaatg ccaccatta aaaaaacaa 
      atcactaca atttgcgac aaccacaaa ttaaccgat 
      gcacagagg aacaaactt aaagtgatg agctccgaa 
      ttcggcatc gaaatgagg aaaccgacg atcagaaaa 
      agtttaact caatgggaa atcaaccga aatcatgtt 
      ctaggcact agcgaggtg tttatgccg gtaaaactt 
      gacaacgtt aattatctg aatcggaat tttacgaat 
      agcgacata agtgagaaa aaaattaaa aaatcgaat 
      agtctttct ttggatttt gccaaatgt gatatccct 
      tcaccaatg agtgtcgac acgacccca atgagcgaa 
      tcgacaacg agcagattc ggcctaagt gtgtcacga 
      ggcgatttt gagagtctt cccacaact gctgaaact 
      caagccact gacgaaggt ttcctgttt gaaggcgtc 
      ccagaatcg agctatgtc ccaatcaag cgaaatttt 
      gacgattcg ttgcagaaa aaagtcttc tcgaagcga 
      gtaacacgc gaggaagcg tctggtctt agcacaaat 
      tgtctgagg ctgtttctt cacgaaagt gtttcgatt 
      ccgttagca acgcagaca aaactactc gataacgaa 
      ttactcaaa tactttgtg gaggatttg caatacttg 
      aagcatttg aatagtctt cgcgattat tttttcctg 
      caagatggc gagtttggg cgcaacatt actgagact 
      ctgttcgag aagttatac gatgtgcat tttcccatc 
      gaactgatc aattgtcaa acgttgcag agtttggtt 
      ttgggagcg ttgcagatg tcaagtaaa tttcaggaa 
      aattcaact tgtttgtct ttcaagatc aatagtctg 
      ccgaagagg tttcattta ggggatcct gatgttttg 
      gattgcttg tcgctgact tataaagtt aaatggcct 
      ttgaatatt ttgctgccg gtggacacg attgagaag 
      tacgatgaa gtgttcaag tttttgctt aaacttaat 
      cggatttcg tgggtgttg aagaaaatt cttttggaa 
      ctgaagatc ttggcgaaa gtctcggga aagaaagag 
      atttatttg atgatgtcg cctcattat cggcgagtg 
      caccaatgc aggcacgtc atgttgcac tttatccaa 
      acattgcaa aactatgtg gttggagag gtgctgcaa 
      tcgagttgg gaagttttc gaacgcaat ttggtcaca 
      gttacaaat ttggatact ttatattcg actcacacc 
      aactacatt aaaacaatt ttattcctc tgtctgctt 
      aatcaaaaa tcggcgcct ttgaggaaa gttattcaa 
      aaaatattc gttgtcatt ttaaaattc tacgatcat 
      ttgaggtca agggcgtgg aaatgcgag aatggaact 
      tacattcat cccaatttt gcgaaattg gagagcatt 
      tttcttaat tttgaagaa tttgttttg tacttgttt 
      aagatagga cggaaggtt gcaaaaagt ggctaccag 
      ccacattta acacagttg cttgatatg ttggatgtt 
      aatggttat tattccaac aaaaattat gttcaagtt 
      acttaa
    mRNA sequence
    • >TC033928 RA
      cagttgtca tttgggtta agtcaaatt tggttaaat 
      ttaaaaacg atggatgaa aattgtgaa aatagtgtg 
      tataatcta atcaccaac ttgtgtgaa acattttct 
      tatgaccag acggtcgtt aagaaactt cgatcaacg 
      gcctatgaa attctgttg aaaaagaga agccacaat 
      caaacaagt cagagacat ttgaaaggt cctgatcct 
      cattgtagt ttgttagct tggcaattt gaacttgcg 
      acaaagtac aatttagtg gatcatggt ttaaaattg 
      aaggaatgt ggagaatat attcaaagg cattacgaa 
      gaaaatatt attgagttt ttgttatgt ttgcgaaat 
      gttccggtt gaaaaagac gattctgtg gattttgag 
      ctacctttt gatgttaaa agtttatac aaaataccc 
      tcgacacct gaaagcgaa tgctcagct ttgattgta 
      gacattgct aaaaactgc tcatcaaca gacgtgtgg 
      actgaggcg tccaaatta acatttagc aaccgaaaa 
      aactgggaa gattttggg catcctgag cctgataag 
      gaaaaaccg tttttgtgt gaattaggg gaattgtct 
      catttatgg gttgagaat ttaccttct ggttatttt 
      tcttgcttc ttgcaagat gagagtact gttgaacca 
      gttgttaaa acagtgagt gaatttgtg aaagatgtc 
      aagtatttg ctagtgggc atggcctct tacttcttc 
      agctatacc gacgaaagt gattttttt ttggttcca 
      aacctaaca gttaaaggg ctatcatca gaagcttta 
      aaatcgtac agcgaggag tttattttt tgtggaaac 
      tgttacaaa gctttacat gaaatttca actccctgc 
      tcacttacg ggggaatac aaacaggac ggttatatt 
      ttcgctgag ctatgccag agcattcag cgttatttg 
      gaaatttat cgtacgaca attatgagt ctatctgac 
      acaattggt ttattaaaa ttgcacgaa cgcactaat 
      caattgcgc aaacaatta actacttta gcttcgatt 
      tgtaaattg ggacctttt gcacagtca gagaggatg 
      ccacatgga gtgttactg ctgaattat ctataccag 
      acagcttta agtctcact gataaaaat gttttattg 
      gtgttgtac gcagttttg ttcccttgc tgtcaggtc 
      tatttcagc aggtttctc caacagtgg cttttggac 
      ggcactgtt aacgatccg tttgaagaa tttttcatt 
      aaaccaaat cttaaatac atatcgaca cgaggcagg 
      acgtactgg accaggagt tacaacatc cgtgaagat 
      atcgttcca gattttcta gcggatttg cgaatggat 
      attctaaac tgcggcaaa tccatgaat ttgctaaaa 
      ttatgctta caatcgagc acattttgc tcgtatcta 
      atgggaaag aaaccgctg attatatct tgttgttta 
      acttcggaa caattatcc ttgttggag caaaacgcg 
      acgagttat taccttgaa attttatcc gaatgtggt 
      ccgaggatt aacctggaa catatcgtt aggaggaat 
      aaagaacag gatccgatc tttatgaat ttaatcgcc 
      aagaaacgt gctgcgacg ttggcgaga cttgaactc 
      gaacgaaag aaagtctta gaagaagaa agggagaaa 
      aagctcgat gaactgacc gcattaaaa gaacagtat 
      gattccgct gttcagcta aaacaagaa agaattgca 
      tcggaaata gaacacgag atcaaacta atccagttt 
      aacttatct attgaaaat actcgcgat aggttaatc 
      gagagagaa gctaatgag ttaatcgat tattacaac 
      aaattgtac gaaatatcg gaccttcgg cgaaaaaaa 
      attgaaacc cacattgag aaactgaaa acttgtttg 
      gaagagcca agtaatttg gaaacaaac tcttcagaa 
      gagtctttc tattccgtt gatgaacca atgaaagag 
      acagaaagt gaagagaaa ttcgatgag tcggagaca 
      caaccagaa gaacctatt cacacttca aaatctctc 
      gatataatc aatgccaac agcgaacca gcccttgaa 
      acatttgcg acagcaaaa aaaatcaaa gaaaaggtt 
      ttaacagaa gaaatggga atcttaatg ccaccatta 
      aaaaaacaa atcactaca atttgcgac aaccacaaa 
      ttaaccgat gcacagagg aacaaactt aaagtgatg 
      agctccgaa ttcggcatc gaaatgagg aaaccgacg 
      atcagaaaa agtttaact caatgggaa atcaaccga 
      aatcatgtt ctaggcact agcgaggtg tttatgccg 
      gtaaaactt gacaacgtt aattatctg aatcggaat 
      tttacgaat agcgacata agtgagaaa aaaattaaa 
      aaatcgaat agtctttct ttggatttt gccaaatgt 
      gatatccct tcaccaatg agtgtcgac acgacccca 
      atgagcgaa tcgacaacg agcagattc ggcctaagt 
      gtgtcacga ggcgatttt gagagtctt cccacaact 
      gctgaaact caagccact gacgaaggt ttcctgttt 
      gaaggcgtc ccagaatcg agctatgtc ccaatcaag 
      cgaaatttt gacgattcg ttgcagaaa aaagtcttc 
      tcgaagcga gtaacacgc gaggaagcg tctggtctt 
      agcacaaat tgtctgagg ctgtttctt cacgaaagt 
      gtttcgatt ccgttagca acgcagaca aaactactc 
      gataacgaa ttactcaaa tactttgtg gaggatttg 
      caatacttg aagcatttg aatagtctt cgcgattat 
      tttttcctg caagatggc gagtttggg cgcaacatt 
      actgagact ctgttcgag aagttatac gatgtgcat 
      tttcccatc gaactgatc aattgtcaa acgttgcag 
      agtttggtt ttgggagcg ttgcagatg tcaagtaaa 
      tttcaggaa aattcaact tgtttgtct ttcaagatc 
      aatagtctg ccgaagagg tttcattta ggggatcct 
      gatgttttg gattgcttg tcgctgact tataaagtt 
      aaatggcct ttgaatatt ttgctgccg gtggacacg 
      attgagaag tacgatgaa gtgttcaag tttttgctt 
      aaacttaat cggatttcg tgggtgttg aagaaaatt 
      cttttggaa ctgaagatc ttggcgaaa gtctcggga 
      aagaaagag atttatttg atgatgtcg cctcattat 
      cggcgagtg caccaatgc aggcacgtc atgttgcac 
      tttatccaa acattgcaa aactatgtg gttggagag 
      gtgctgcaa tcgagttgg gaagttttc gaacgcaat 
      ttggtcaca gttacaaat ttggatact ttatattcg 
      actcacacc aactacatt aaaacaatt ttattcctc 
      tgtctgctt aatcaaaaa tcggcgcct ttgaggaaa 
      gttattcaa aaaatattc gttgtcatt ttaaaattc 
      tacgatcat ttgaggtca agggcgtgg aaatgcgag 
      aatggaact tacattcat cccaatttt gcgaaattg 
      gagagcatt tttcttaat tttgaagaa tttgttttg 
      tacttgttt aagatagga cggaaggtt gcaaaaagt 
      ggctaccag ccacattta acacagttg cttgatatg 
      ttggatgtt aatggttat tattccaac aaaaattat 
      gttcaagtt acttaaatt ttatcaagg ttgaataaa 
      tgcttaaaa ctgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY