Status
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Gene information
General information
TC008602 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15669783..15680218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008602
    iBeetle screen iB_09265

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 2

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008602 RA
      MPTTPPQIS PVEEQEKYL QDALGVVKA QAFHMKRAL 
      DKNKLMDAL KNASAMLAE LRTSLLSPK SYYELYMAI 
      TDELRHLEL YLLDEFQKG RKVTDLYEL VQYAGNIVP 
      RLYLLITVG LVYIKTNSA LRRDLLKDL VEMCRGVQH 
      PLRGLFLRN YLLQCTRNV LPDSPDSDA DNPEGTVRD 
      SIDFVLMNF AEMNKLWVR MQHQGHSRE RQHRERERE 
      ELKILVGTN LVRLSQLES VTLEKYQKL VLPGILEQV 
      VSCRDAIAQ EYLMECIIQ VFPDEFHIK TLNPFLKSC 
      AELESGVNV KNIVISLME RLALFSQRS DALGNEGAT 
      ILQQVQLFE VFSDQVASI IANRQYLPP EDMIALQVA 
      LVNLALKCY PDRIDYIDK VMLNSVEVF QRLGLEHLE 
      SNSLVAKEL QKLLKIPLD NYNNLLTIL KLKHYAGLM 
      QHLDYAGRK SLSIYILNN ALDNDTIVP TQEETEQAL 
      NLLSPLVND KEEQPLGEL DLEELAEEQ CLLARFIHQ 
      LKSDVADDQ YLILTAARK ILGGGGPQR IKYTFPPIL 
      FQAYLLAYK YKEIKDEKW EKKCQKIFQ FCHSAITTL 
      VKAELAELP LRLFLQGAL AIDQIGFEN HETVAYEFM 
      SQAFSLYED EISDSKAQL AAITLIVGT LEQISCFSE 
      ENSDPLRTQ CALAASKLL KKPDQCRGV ATCSHLFWS 
      GKSLASNRE EAHDGKRVV ECLKKGLRI AKQCMDVSV 
      QVQLFVELL NHYIYFFEK GNDQVSVQV LNQVIGKIK 
      EELPNLESS DETDQITKH FNNTLEHLR ARLETPDAD 
      GVSYEGLEL
    CDS sequence
    • >TC008602 RA
      atgccaacc actccaccc caaatatct cccgtggag 
      gaacaggaa aagtacctt caggatgcc ttgggggtg 
      gtcaaagca caggctttt catatgaaa agagctctc 
      gataaaaac aaactgatg gatgcattg aagaacgct 
      agtgcgatg cttgccgaa ctgcggacg tctttatta 
      tcacccaag agttattat gagctttac atggccatc 
      acagacgag ttgcgccac ttggaactc tatcttttg 
      gatgagttt caaaagggg cgcaaagtt acagatttg 
      tatgaactt gtacaatac gccgggaac attgtcccc 
      cgactctat cttttaatc acagtcggt ttagtttat 
      attaaaaca aacagcgcg ttaagacgc gacctactc 
      aaggattta gtagaaatg tgtagagga gtgcaacat 
      ccattacgc ggccttttc ttgcgtaat tacctgctg 
      cagtgtacg aggaacgtc ctgccagat agccctgat 
      tctgacgct gataatcct gagggaact gtgagggat 
      tctattgat tttgttttg atgaatttc gccgaaatg 
      aacaaattg tgggtcagg atgcagcat caaggccat 
      tcgagagaa cgacaacat cgggaaagg gaaagagaa 
      gaactcaaa attctagtg gggacaaat ctggttagg 
      ctgagccaa ctagagtct gttacattg gagaagtac 
      caaaaactc gttttgccc gggatttta gaacaggtt 
      gtgagctgt cgggacgcc atcgcgcag gagtacttg 
      atggagtgc ataatccaa gtgtttcct gacgagttt 
      cacatcaaa accctcaat ccgttccta aaatcatgc 
      gccgagttg gaatctggt gtcaatgtg aaaaacatt 
      gtcataagt ttaatggaa cgtttggct ttgttcagt 
      caaagatct gacgctttg ggcaacgaa ggagcaaca 
      attctgcaa caagtccaa ctctttgaa gttttttcg 
      gatcaagta gcttcgatc atagccaat cggcagtat 
      ttaccaccg gaagacatg atcgcactg caagtagct 
      ttggtgaat ttggccttg aagtgttac cctgatcgt 
      attgattat atcgataag gtgatgttg aatagtgtt 
      gaagttttc caacgtttg ggacttgaa catcttgaa 
      tcgaactcg ttggtggcc aaagagttg caaaaatta 
      ttaaaaata ccattggat aattataat aatttatta 
      accatttta aaattgaag cattatgcc ggtttgatg 
      caacattta gattacgcg ggacggaaa tctttgagt 
      atttatatc ttgaataat gctctagat aacgacacc 
      attgtaccg acgcaagaa gagactgaa caagcttta 
      aatttgttg agtccttta gttaacgat aaggaagaa 
      caaccactt ggagagctt gatttggaa gaattggct 
      gaggagcag tgtttattg gctagattt atccaccaa 
      ttgaaatct gacgtcgct gacgaccaa tacctcatc 
      ctcacggca gctcgcaag atcttgggc ggcggtggt 
      cctcaacgt atcaaatac acgtttccc ccgatcctc 
      ttccaggcc tatctcctc gcctacaaa tacaaagaa 
      atcaaagac gagaaatgg gaaaagaag tgtcagaaa 
      attttccag ttttgccat tccgccatc acgacctta 
      gtcaaggcc gagttggca gaactacct ttgcgctta 
      ttcctccag ggggcgctc gcgatcgac caaatcggg 
      ttcgaaaac cacgagact gtggcttac gaattcatg 
      tctcaagca ttttcactt tatgaagat gaaatttcc 
      gattcgaag gctcaatta gctgccata accctaatc 
      gtagggact ttggaacaa atcagttgt ttctcggaa 
      gagaattcg gatccgttg cgaacgcag tgtgctttg 
      gcagcgagc aagctattg aagaaacct gatcagtgc 
      cgaggtgtc gccacctgt tctcatttg ttttggagc 
      gggaagagt ttggcgagt aacagggag gaggcgcac 
      gacggaaag agagtggtg gagtgtctc aaaaagggg 
      ctgcggata gccaagcag tgcatggat gtcagcgtg 
      caagttcag ttgtttgtt gagttgttg aatcattat 
      atctatttc tttgagaaa gggaacgat caagtgagt 
      gtgcaggtt ctgaaccaa gttattgga aagattaaa 
      gaggagttg ccgaattta gagagttct gatgagaca 
      gatcagatt actaaacat tttaataac actttggaa 
      catctgagg gcaagactt gagacgcct gatgcagat 
      ggagtttcg tacgaggga ctggaactt tga
    mRNA sequence
    • >TC008602 RA
      tgtcagcaa gttaacagt gctaccaac aacagtcgt 
      tctgtactt gtttttatc gggacgaat acgttaatt 
      agctattgc aataaatga attataagt ttagataac 
      agtgtttat catttgttc gtttgttaa atttttaca 
      gctcgtgtt cagataatg cgtttggga catttcact 
      aaaagtttt gaccatagg ttttgacca aaaatccct 
      aactgaaag aggaggttt ggcgtttcg acttatcaa 
      aaatgccaa ccactccac cccaaatat ctcccgtgg 
      aggaacagg aaaagtacc ttcaggatg ccttggggg 
      tggtcaaag cacaggctt ttcatatga aaagagctc 
      tcgataaaa acaaactga tggatgcat tgaagaacg 
      ctagtgcga tgcttgccg aactgcgga cgtctttat 
      tatcaccca agagttatt atgagcttt acatggcca 
      tcacagacg agttgcgcc acttggaac tctatcttt 
      tggatgagt ttcaaaagg ggcgcaaag ttacagatt 
      tgtatgaac ttgtacaat acgccggga acattgtcc 
      cccgactct atcttttaa tcacagtcg gtttagttt 
      atattaaaa caaacagcg cgttaagac gcgacctac 
      tcaaggatt tagtagaaa tgtgtagag gagtgcaac 
      atccattac gcggccttt tcttgcgta attacctgc 
      tgcagtgta cgaggaacg tcctgccag atagccctg 
      attctgacg ctgataatc ctgagggaa ctgtgaggg 
      attctattg attttgttt tgatgaatt tcgccgaaa 
      tgaacaaat tgtgggtca ggatgcagc atcaaggcc 
      attcgagag aacgacaac atcgggaaa gggaaagag 
      aagaactca aaattctag tggggacaa atctggtta 
      ggctgagcc aactagagt ctgttacat tggagaagt 
      accaaaaac tcgttttgc ccgggattt tagaacagg 
      ttgtgagct gtcgggacg ccatcgcgc aggagtact 
      tgatggagt gcataatcc aagtgtttc ctgacgagt 
      ttcacatca aaaccctca atccgttcc taaaatcat 
      gcgccgagt tggaatctg gtgtcaatg tgaaaaaca 
      ttgtcataa gtttaatgg aacgtttgg ctttgttca 
      gtcaaagat ctgacgctt tgggcaacg aaggagcaa 
      caattctgc aacaagtcc aactctttg aagtttttt 
      cggatcaag tagcttcga tcatagcca atcggcagt 
      atttaccac cggaagaca tgatcgcac tgcaagtag 
      ctttggtga atttggcct tgaagtgtt accctgatc 
      gtattgatt atatcgata aggtgatgt tgaatagtg 
      ttgaagttt tccaacgtt tgggacttg aacatcttg 
      aatcgaact cgttggtgg ccaaagagt tgcaaaaat 
      tattaaaaa taccattgg ataattata ataatttat 
      taaccattt taaaattga agcattatg ccggtttga 
      tgcaacatt tagattacg cgggacgga aatctttga 
      gtatttata tcttgaata atgctctag ataacgaca 
      ccattgtac cgacgcaag aagagactg aacaagctt 
      taaatttgt tgagtcctt tagttaacg ataaggaag 
      aacaaccac ttggagagc ttgatttgg aagaattgg 
      ctgaggagc agtgtttat tggctagat ttatccacc 
      aattgaaat ctgacgtcg ctgacgacc aatacctca 
      tcctcacgg cagctcgca agatcttgg gcggcggtg 
      gtcctcaac gtatcaaat acacgtttc ccccgatcc 
      tcttccagg cctatctcc tcgcctaca aatacaaag 
      aaatcaaag acgagaaat gggaaaaga agtgtcaga 
      aaattttcc agttttgcc attccgcca tcacgacct 
      tagtcaagg ccgagttgg cagaactac ctttgcgct 
      tattcctcc agggggcgc tcgcgatcg accaaatcg 
      ggttcgaaa accacgaga ctgtggctt acgaattca 
      tgtctcaag cattttcac tttatgaag atgaaattt 
      ccgattcga aggctcaat tagctgcca taaccctaa 
      tcgtaggga ctttggaac aaatcagtt gtttctcgg 
      aagagaatt cggatccgt tgcgaacgc agtgtgctt 
      tggcagcga gcaagctat tgaagaaac ctgatcagt 
      gccgaggtg tcgccacct gttctcatt tgttttgga 
      gcgggaaga gtttggcga gtaacaggg aggaggcgc 
      acgacggaa agagagtgg tggagtgtc tcaaaaagg 
      ggctgcgga tagccaagc agtgcatgg atgtcagcg 
      tgcaagttc agttgtttg ttgagttgt tgaatcatt 
      atatctatt tctttgaga aagggaacg atcaagtga 
      gtgtgcagg ttctgaacc aagttattg gaaagatta 
      aagaggagt tgccgaatt tagagagtt ctgatgaga 
      cagatcaga ttactaaac attttaata acactttgg 
      aacatctga gggcaagac ttgagacgc ctgatgcag 
      atggagttt cgtacgagg gactggaac tttgaatgc 
      gatcgtatt gaattattg ttaattgta ttgtttttt 
      aatataata tgtatatta tgatctatc gaaataaaa 
      ataatggca ccaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY