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Gene information
General information
TC010117 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14517719..14522831
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010117
    iBeetle screen iB_09262

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010117 RA
      MLVAVIGAG AAGLASARH LSSQKIKCE VFEMTAELG 
      GTWVYTDKV GTDCYGYPI HTAMYKNLR INLPKEASG 
      FPDFPIPEE DESYVSQEV VLRFLNGYA DHFKLRQFI 
      KFNHVVAEI RPFGNKWQV KALNKITQQ TTITVYDSV 
      MVCNGHYNS PIFPTLQGV KKFNGRIIH SYNYRANSP 
      YKNQRVLII GGGPSGLDI GTQISEVAK QVVISHRKK 
      LPNGEYPPN VIKKPEVLQ VNNEGQVEF ADGTTFAFD 
      SILYCTGYK YNFPFLHDD CGVTVEEFY HVKPLYKHM 
      IHIEKPTMC FIGIPFNVC AFQMMDIQV IINICHFLF 
      SYHEN
    CDS sequence
    • >TC010117 RA
      atgctagtc gcggttatt ggcgccggt gctgcaggt 
      ctcgcatct gcaagacat ctttcatcc caaaaaatc 
      aagtgtgaa gtgtttgaa atgacagct gaattaggt 
      ggtacttgg gtttacacc gataaggtg ggaacggac 
      tgttacggg tatcccata cacacagcc atgtataaa 
      aatttaagg ataaatttg ccaaaggaa gcctcgggt 
      tttccagat tttccaatt ccggaagag gatgaatct 
      tatgtgtca caggaagtt gttctacgt ttcctaaac 
      ggttatgcg gatcacttt aaattaaga caatttata 
      aaattcaac cacgtagtc gctgaaatt cgcccattt 
      ggcaataag tggcaagtg aaagcacta aataaaatc 
      acccaacag acaaccata acagtttat gacagtgtt 
      atggtctgt aacggccac tacaactca ccgattttt 
      ccaaccttg caaggagtc aaaaaattc aatgggcgc 
      atcatccat agctacaat taccgcgca aacagcccg 
      tataaaaat caaagagta ttgataata ggtggagga 
      ccatcgggc ctcgatatt ggtacccaa atttcagaa 
      gtggcaaaa caagtcgta atcagtcac cgaaaaaaa 
      ttaccaaat ggtgaatat ccacccaat gtgataaaa 
      aagcctgaa gtcctccaa gtcaacaac gaaggacag 
      gtcgagttt gctgatggg actactttt gcgtttgat 
      agtatttta tattgcacg ggatataag tacaacttt 
      ccctttctt catgacgac tgtggggtt acagttgag 
      gaattctat cacgttaaa cccctttac aaacacatg 
      atacacatt gagaaaccc acaatgtgt tttattggc 
      attccgttt aatgtttgt gcgtttcaa atgatggat 
      attcaagta attataaat atttgtcat ttcctcttt 
      agttatcat gaaaattaa
    mRNA sequence
    • >TC010117 RA
      cgtataatg tcgaaaagg cttgtggct acggtttta 
      agagtgtca ttctttttg aaagctcaa gtattttaa 
      aaatgctag tcgcggtta ttggcgccg gtgctgcag 
      gtctcgcat ctgcaagac atctttcat cccaaaaaa 
      tcaagtgtg aagtgtttg aaatgacag ctgaattag 
      gtggtactt gggtttaca ccgataagg tgggaacgg 
      actgttacg ggtatccca tacacacag ccatgtata 
      aaaatttaa ggataaatt tgccaaagg aagcctcgg 
      gttttccag attttccaa ttccggaag aggatgaat 
      cttatgtgt cacaggaag ttgttctac gtttcctaa 
      acggttatg cggatcact ttaaattaa gacaattta 
      taaaattca accacgtag tcgctgaaa ttcgcccat 
      ttggcaata agtggcaag tgaaagcac taaataaaa 
      tcacccaac agacaacca taacagttt atgacagtg 
      ttatggtct gtaacggcc actacaact caccgattt 
      ttccaacct tgcaaggag tcaaaaaat tcaatgggc 
      gcatcatcc atagctaca attaccgcg caaacagcc 
      cgtataaaa atcaaagag tattgataa taggtggag 
      gaccatcgg gcctcgata ttggtaccc aaatttcag 
      aagtggcaa aacaagtcg taatcagtc accgaaaaa 
      aattaccaa atggtgaat atccaccca atgtgataa 
      aaaagcctg aagtcctcc aagtcaaca acgaaggac 
      aggtcgagt ttgctgatg ggactactt ttgcgtttg 
      atagtattt tatattgca cgggatata agtacaact 
      ttccctttc ttcatgacg actgtgggg ttacagttg 
      aggaattct atcacgtta aaccccttt acaaacaca 
      tgatacaca ttgagaaac ccacaatgt gttttattg 
      gcattccgt ttaatgttt gtgcgtttc aaatgatgg 
      atattcaag taattataa atatttgtc atttcctct 
      ttagttatc atgaaaatt aatgtccta cgtacgttt 
      ttattgcca atatctcaa cgggacgat ggagttgcc 
      acccaaaga gttaatgta taaagatgt cgaagagga 
      tttagctat tcgcaaagc taaaggtta ctccaaaag 
      ccaaatgca taaactggc aagagacca acaaacgta 
      tttcgacag tttggcggt tagtgcaaa aatcacgtc 
      tgtacttcc ggtgatgtg taaaatatg gaaccacgt 
      tgaaataag tattttaag tgattttgg aagtttcag 
      aaaacataa cttcactgt tctcaatga tgagtcttt 
      tgttgtaga gcagagcgt catacagtt tagtatcaa 
      gaattgtaa ttagtccat ttttttaat ttttattcg 
      taatcgcaa atgaaatat gtgataatt tttggcaaa 
      tctaatttt tttattatt ttacaaggt tatttgaag 
      atagccgct gccttctac tgcacttta ttttattag 
      ccaatgttc attttatgg gtttgccga tcgtaccgg 
      acgccagaa tttttgcta aaagtctgg aatttatta 
      ctttggatt accaactaa taagtcatt tgtggacca 
      tctggttcc aactgagtt tgtcaataa attaaaaag 
      atcagaagt gaccttaaa aaccctaaa aaccagagt 
      tgccttaat tattattgt tttgagtac tgataatag 
      tagttaaaa ttcatcaat gtttctttg ttctttgat 
      tgcaatgca agatttaca ttattttcc tattaatta 
      tagaataat ttttgatag attacaatt tcttccgta 
      attcgtaac cttgattat tttcccacg caagcggcg 
      atttctctc aataggtgg tggagaagc tcgaccctt 
      atcggtaaa tttaaaatt attattcaa gttgactag 
      acgcctgcg ttgcgattt ttttcctga ctgtggcca 
      aaattgtaa agcattaaa aaaaatcat cgaactctt 
      catcacagg taggcaata ccatttttc ataaatcat 
      gactaattt gtactggga tggagttag gtctcaaat 
      aattttttt tatttttcc taacgagta aaaaaaaat 
      aaaaaatgt atcaagct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY