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Gene information
General information
TC033859 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13892271..13894045
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033859
    iBeetle screen iB_09256

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    vitellarium size decreased -
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 2
    vitellogenic egg chamber potentially fused - Number of animals showing the phenotype: 2
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 2
    vitellogenic egg chamber potentially degenerated - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033859 RA
      MFRPISVKL ETVDEYLQQ NDYSALELY LKKEFRLEK 
      DETGVPLKI EFSTQAVDL LHKILKTLS EQSLSKNHE 
      GIIKELFRS LRNYVINSD VQNYLVNDE NFLPNVSNF 
      MNLQSEVIQ NAFQIILQF LFNLIISNK SAVDRVYDV 
      FYPTILSLL KDKKNIYET SALLYNMSL YKELNMDFD 
      TYSFLLELY DSHNSVEYL YFMLEKLIS DCNFWNYYL 
      KFESNKRAA VLGVIRDKQ IKGEALNIP VTGLKVLTN 
      QFVASGNII FQTVQNKDS SLEAYEISQ LVQVISSFC 
      SNEDYLKVL QSDKDLLIN SGVLLINIH RLGKESENC 
      FTSIQNLSE IREPNKNIQ EHPAFGFKA DLIRIIGNL 
      CWKNKIMQD LTREAELIP VILDCCNMD ARNPFIMQW 
      VILAVRNLC ENNMENQKI IAELHQQGT VSSSVLQEL 
      GVTLESDGD RAIKIVSLD SLRKQK
    CDS sequence
    • >TC033859 RA
      atgtttaga ccaatatcc gtaaaactg gaaactgtc 
      gatgaatac ctgcagcag aatgattat tccgctctt 
      gaactttat ttaaagaaa gaatttagg ctggaaaag 
      gacgagaca ggtgtcccc ctaaagatt gaattttca 
      actcaagct gtggatttg ctacataaa attttaaag 
      acactttct gagcaaagc ctaagcaaa aatcatgaa 
      ggaatcata aaagaattg tttaggagt ctaaggaat 
      tatgttata aattcagac gttcaaaat tatttagtt 
      aatgacgaa aattttcta ccgaatgtg tccaatttt 
      atgaactta cagtctgaa gtgatacaa aatgcattc 
      caaattatt ttacaattt ttgtttaat ttaataata 
      tcaaataaa agtgctgtg gatagagta tatgatgtg 
      ttttatcct acaatactt tcactcttg aaagacaag 
      aaaaacatt tacgaaacg tctgccctt ttatataac 
      atgtcttta tacaaggaa ttgaacatg gattttgac 
      acgtattct tttctcctt gaactttac gactctcac 
      aatagtgta gaatatttg tacttcatg ttagaaaaa 
      ctaataagt gattgtaat ttttggaat tattactta 
      aaatttgag agtaataag agggcagca gtgttgggc 
      gtaatcaga gataaacag attaaagga gaggcatta 
      aatatacca gtaacgggt ttaaaagtg ttaactaac 
      caatttgta gcgtctggt aatattatt tttcaaaca 
      gttcaaaat aaagattca agtttggaa gcttatgag 
      atatcgcag cttgtgcaa gtcatatcg tctttctgt 
      agtaatgaa gattattta aaagtgcta caaagcgat 
      aaagattta ttaatcaac tctggtgtt ttattaatc 
      aatatccac agactagga aaagaaagt gaaaattgt 
      tttacttca atacagaat ttgtctgaa attagagaa 
      cctaataaa aacatccaa gaacatcca gctttcgga 
      tttaaggcg gacttgata aggataatt ggtaatttg 
      tgctggaaa aacaaaatt atgcaggac ttgacaaga 
      gaagccgag ctcattcca gttattttg gactgttgt 
      aacatggac gcaagaaat cctttcata atgcaatgg 
      gttatattg gccgtgcgt aatttgtgc gaaaacaac 
      atggaaaat cagaaaatt attgctgag ttgcatcaa 
      caaggaact gtttcttct tcagtgctg caagaatta 
      ggagtaacg ttagagagt gatggcgac agagctata 
      aaaattgta tcattagat tcattgcga aaacagaaa 
      taa
    mRNA sequence
    • >TC033859 RA
      gtagggttg gtgcttgat tttcaaatt tgacaccta 
      acctttaaa aattagcca aaatagtat aaaaattgt 
      gagcttttt cgacgtgaa aataaatta tgtttagac 
      caatatccg taaaactgg aaactgtcg atgaatacc 
      tgcagcaga atgattatt ccgctcttg aactttatt 
      taaagaaag aatttaggc tggaaaagg acgagacag 
      gtgtccccc taaagattg aattttcaa ctcaagctg 
      tggatttgc tacataaaa ttttaaaga cactttctg 
      agcaaagcc taagcaaaa atcatgaag gaatcataa 
      aagaattgt ttaggagtc taaggaatt atgttataa 
      attcagacg ttcaaaatt atttagtta atgacgaaa 
      attttctac cgaatgtgt ccaatttta tgaacttac 
      agtctgaag tgatacaaa atgcattcc aaattattt 
      tacaatttt tgtttaatt taataatat caaataaaa 
      gtgctgtgg atagagtat atgatgtgt tttatccta 
      caatacttt cactcttga aagacaaga aaaacattt 
      acgaaacgt ctgcccttt tatataaca tgtctttat 
      acaaggaat tgaacatgg attttgaca cgtattctt 
      ttctccttg aactttacg actctcaca atagtgtag 
      aatatttgt acttcatgt tagaaaaac taataagtg 
      attgtaatt tttggaatt attacttaa aatttgaga 
      gtaataaga gggcagcag tgttgggcg taatcagag 
      ataaacaga ttaaaggag aggcattaa atataccag 
      taacgggtt taaaagtgt taactaacc aatttgtag 
      cgtctggta atattattt ttcaaacag ttcaaaata 
      aagattcaa gtttggaag cttatgaga tatcgcagc 
      ttgtgcaag tcatatcgt ctttctgta gtaatgaag 
      attatttaa aagtgctac aaagcgata aagatttat 
      taatcaact ctggtgttt tattaatca atatccaca 
      gactaggaa aagaaagtg aaaattgtt ttacttcaa 
      tacagaatt tgtctgaaa ttagagaac ctaataaaa 
      acatccaag aacatccag ctttcggat ttaaggcgg 
      acttgataa ggataattg gtaatttgt gctggaaaa 
      acaaaatta tgcaggact tgacaagag aagccgagc 
      tcattccag ttattttgg actgttgta acatggacg 
      caagaaatc ctttcataa tgcaatggg ttatattgg 
      ccgtgcgta atttgtgcg aaaacaaca tggaaaatc 
      agaaaatta ttgctgagt tgcatcaac aaggaactg 
      tttcttctt cagtgctgc aagaattag gagtaacgt 
      tagagagtg atggcgaca gagctataa aaattgtat 
      cattagatt cattgcgaa aacagaaat aaaatgtaa 
      tttatgaat tatgcttag ttaataaag tttagaaaa 
      ctga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY