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Gene information
General information
TC010161 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13794890..13796305
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010161
    iBeetle screen iB_09255

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010161 RA
      MPLSCRFYR EKYPEVEDV VMVNVRAIA EMGAYVHLL 
      EYKNIEGMI LLSELSRRR IRSINKLIR VGKTEPVVV 
      IRVDKDKGY IDLSKRRVS AEDVEKCTE RFAKAKAVN 
      SILRHVAEL LKYESDEQL EELYQKTAW YFEEKYKKN 
      KASAYDFFK QAVSDPSIL AECQLDDRT KEVLLSNIK 
      RKLTSQAVK IRADIECAC YGYEGIDAV KTALKSGLS 
      LSTDELPIK INLIAPPLY VMTTSTPEK QDGLKILND 
      AIEKIKATI SELGGVFNV QMAPKVVTA TDEAELQRQ 
      MERAELENT EVAGDDDDE EDGDEGQVY NENDNESEP 
      EED
    CDS sequence
    • >TC010161 RA
      atgcctctc tcctgtaga ttttacagg gaaaagtac 
      cctgaagtc gaggatgtg gtgatggtg aacgttcga 
      gccattgcc gaaatgggg gcttacgtt catttactg 
      gaatataag aacattgaa ggcatgatt ttgctttcc 
      gaattgtcg agaagacgt attcgttcc ataaataaa 
      ttaattaga gtaggtaaa acggaaccc gttgttgta 
      ataagagtg gacaaggac aagggttat attgacttg 
      tcaaaaagg cgtgttagt gctgaggat gtcgagaag 
      tgcactgaa agatttgcg aaagcaaag gccgtcaat 
      tcgattctt agacacgtc gctgaattg ctcaaatat 
      gagtcagat gagcagttg gaggaattg taccaaaaa 
      actgcgtgg tactttgag gaaaagtac aagaaaaat 
      aaagcaagt gcttatgat ttcttcaag caggctgtc 
      tctgatccc agtatattg gccgaatgt cagttggat 
      gatagaacc aaggaagta ttgttgagc aacatcaag 
      aggaaactg acttctcaa gcagtcaaa atccgtgct 
      gatattgaa tgtgcatgt tacggctat gaaggaatt 
      gacgctgtg aagacagcc ttgaagtcc ggtttgtcg 
      ctttcaact gatgaattg ccaattaaa atcaatctc 
      attgctcct cctctctac gtaatgacg acttctacg 
      cctgaaaaa caagacggg ctgaaaata ctaaacgat 
      gcaattgag aaaataaaa gccacaatt tctgaatta 
      ggtggcgtt tttaacgtt cagatggct cctaaagtt 
      gtgacagct accgatgaa gctgagctg caaagacaa 
      atggaaaga gccgaattg gaaaacact gaagttgcg 
      ggcgacgac gacgatgaa gaagacgga gatgaggga 
      caagtttac aatgagaat gataatgaa tctgaacct 
      gaagaagat taa
    mRNA sequence
    • >TC010161 RA
      atttgatgc gttgaataa tacagagaa aagcttgca 
      ggttgtggc tgtgctgtc acacgtgaa gttatttcg 
      gaatttcat cattttttc atatatttt gataataat 
      ataggtaaa aatgcctct ctcctgtag attttacag 
      ggaaaagta ccctgaagt cgaggatgt ggtgatggt 
      gaacgttcg agccattgc cgaaatggg ggcttacgt 
      tcatttact ggaatataa gaacattga aggcatgat 
      tttgctttc cgaattgtc gagaagacg tattcgttc 
      cataaataa attaattag agtaggtaa aacggaacc 
      cgttgttgt aataagagt ggacaagga caagggtta 
      tattgactt gtcaaaaag gcgtgttag tgctgagga 
      tgtcgagaa gtgcactga aagatttgc gaaagcaaa 
      ggccgtcaa ttcgattct tagacacgt cgctgaatt 
      gctcaaata tgagtcaga tgagcagtt ggaggaatt 
      gtaccaaaa aactgcgtg gtactttga ggaaaagta 
      caagaaaaa taaagcaag tgcttatga tttcttcaa 
      gcaggctgt ctctgatcc cagtatatt ggccgaatg 
      tcagttgga tgatagaac caaggaagt attgttgag 
      caacatcaa gaggaaact gacttctca agcagtcaa 
      aatccgtgc tgatattga atgtgcatg ttacggcta 
      tgaaggaat tgacgctgt gaagacagc cttgaagtc 
      cggtttgtc gctttcaac tgatgaatt gccaattaa 
      aatcaatct cattgctcc tcctctcta cgtaatgac 
      gacttctac gcctgaaaa acaagacgg gctgaaaat 
      actaaacga tgcaattga gaaaataaa agccacaat 
      ttctgaatt aggtggcgt ttttaacgt tcagatggc 
      tcctaaagt tgtgacagc taccgatga agctgagct 
      gcaaagaca aatggaaag agccgaatt ggaaaacac 
      tgaagttgc gggcgacga cgacgatga agaagacgg 
      agatgaggg acaagttta caatgagaa tgataatga 
      atctgaacc tgaagaaga ttaaacaaa cctcaaacg 
      ttttatgat taaatacca tgtgtatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY