Status
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Gene information
General information
TC033838
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008619,TC008620
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13170563..13191827
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033838
    iBeetle screen iB_09253

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    yolk late potentiallydegenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033838 RA
      MALKSEMKE EHGRSDSYR VATIPSSYD DNKTADGRP 
      KSRRKNKKV RKADDLDDL KQELDIDYH KITPEELYQ 
      RFQTHPENG LSHAKAKEN LERDGPNAL TPPKTTPEW 
      VKFCKNLFG GFALLLWIG AILCFIAYS IQASTVEEP 
      ADDNLYLGI VLAAVVIVT GIFSYYQES KSSKIMESF 
      KNMVPQFAT VIREGEKLT LRAEDLVLG DVVEVKFGD 
      RIPADIRII ESRGFKVDN SSLTGESEP QSRSPEFTH 
      ENPLETKNL AFFSTNAVE GTAKGVVIS CGDNTVMGR 
      IAGLASGLD TGETPIAKE IHHFIHLIT GVAVFLGVT 
      FFVIAFILG YHWLDAVIF LIGIIVANV PEGLLATVT 
      VCLTLTAKR MASKNCLVK NLEAVETLG STSTICSDK 
      TGTLTQNRM TVAHMWFDN QIIEADTTE DQSGVQYDR 
      TSPGFKALS RIATLCNRA EFKGGQNDV PILKREVNG 
      DASEAALLK CMELALGDV MSIRRKNKK VCEIPFNST 
      NKYQVSIHE NEDASDPRH ILVMKGAPE RILERCSTI 
      FICGKEKVL DEEMKEAFN NAYLELGGL GERVLGFCD 
      FMLPTDKYP IGYKFNCDD PNFPLDGLR FVGLMSMID 
      PPRAAVPDA VAKCRSAGI KVIMVTGDH PITAKAIAK 
      SVGIISEGN ETVEDIAQR LNIPVSEVN PREAKAAVV 
      HGSDLRDLS SDQLDEILR YHTEIVFAR TSPQQKLII 
      VEGCQRMGA IVAVTGDGV NDSPALKKA DIGVAMGIA 
      GSDVSKQAA DMILLDDNF ASIVTGVEE GRLIFDNLK 
      KSIAYTLTS NIPEISPFL AFILCDIPL PLGTVTILC 
      IDLGTDMVP AISLAYEEA ESDIMKRPP RDPQNDKLV 
      NDRCCFLHF FYSNNVVVY FVLIFWTNC IYLQYIWGF 
      LTLITGCTD A
    CDS sequence
    • >TC033838 RA
      atggctttg aagagcgag atgaaggaa gagcacggc 
      cgttctgac tcgtatcgg gtggcaact attccgtcc 
      agttatgat gacaacaaa acggctgat ggtagacca 
      aagtcacgg aggaaaaat aagaaggtc aggaaagcg 
      gacgattta gatgatttg aaacaagaa ttggacatc 
      gattatcat aaaatcacc ccagaagaa ttatatcag 
      agattccag acacatcca gaaaatggc ctcagtcat 
      gcgaaagcg aaagagaat ttggaacgg gacggaccc 
      aatgcactc acaccccca aagactacc cccgaatgg 
      gtgaaattt tgtaaaaat ctcttcggg ggtttcgct 
      ctcttattg tggatcggc gccatcctc tgcttcata 
      gcctattct attcaggct agcaccgtg gaggaacca 
      gccgatgat aatctttat cttggcatc gtcttagct 
      gccgttgtt atcgttaca ggtatattt tcttattat 
      caagaaagc aagagttcg aagattatg gagtcgttc 
      aaaaacatg gtcccccaa ttcgctaca gtgatccgc 
      gagggtgaa aagctgacc ctccgcgcg gaggacctg 
      gtactgggc gacgtggtc gaggtgaaa ttcggtgac 
      agaatccca gccgatatc cgaatcatc gaatctcgc 
      ggcttcaaa gtagacaac tcatccttg acaggcgaa 
      tccgaaccg cagtcccgc agtccggag ttcactcac 
      gagaaccct ctcgaaacg aaaaacttg gcgttcttc 
      tcgaccaac gccgtcgaa ggcactgcc aaaggtgtt 
      gtgattagt tgtggtgac aataccgtg atgggtcgc 
      atcgccggt ctcgcctcc ggtctggac accggcgag 
      acgcccatc gccaaagaa atccatcat ttcattcac 
      ctcattact ggcgtggct gttttcctc ggagttacc 
      ttcttcgta atcgccttc atcctcggc taccactgg 
      ctcgacgct gttattttc ctcatcggt attatcgtg 
      gcgaacgtg cccgagggg ctcctcgcc accgtcacc 
      gtgtgtctc accctcact gctaagagg atggcttcc 
      aagaactgc ctcgtgaag aatctcgag gccgtagag 
      accctcggc tccacaagc acgatctgc tcggacaag 
      accggaact ttgacccaa aaccggatg acggtagca 
      cacatgtgg ttcgacaat cagatcatt gaagccgac 
      accactgaa gaccagtcg ggagtccaa tacgaccgc 
      acaagtcca ggattcaaa gctttgtcg cgcattgcc 
      acactttgc aaccgggct gagttcaaa ggggggcag 
      aacgacgtc ccgatcctt aaacgcgaa gtcaacgga 
      gacgcctct gaagccgct ctcctcaaa tgcatggaa 
      ctggctctg ggcgacgtg atgtccatc agacgcaag 
      aacaagaaa gtttgcgaa attcccttc aactcgacc 
      aacaaatac caagtttcc atccacgag aacgaggac 
      gcgagcgat cctcgccat atccttgtg atgaagggc 
      gctcctgaa cgaatcctc gaacgctgc agcacgatc 
      ttcatctgc ggcaaggag aaagtcctg gatgaggaa 
      atgaaggaa gctttcaat aacgcctac ttggagttg 
      ggtggtttg ggcgagcgt gtgctcggc ttctgcgat 
      tttatgttg cccactgat aagtaccca attgggtac 
      aaattcaat tgcgatgac cccaacttc ccgttggat 
      ggtttgaga tttgttggc ttgatgtcc atgattgat 
      cctcccaga gctgcagtg cctgacgcc gttgctaaa 
      tgcagaagt gccggtatt aaggtcatt atggtgacg 
      ggagatcac ccgattacg gccaaggct attgcaaag 
      tcggttggg attatttcg gagggtaac gaaacggtt 
      gaagatatt gctcaacgg ttgaatatt cctgtctcg 
      gaagtcaac ccgagggaa gccaaagct gccgttgtt 
      cacggatct gatctcaga gacctatct tccgatcaa 
      ttagacgaa attttgaga taccacact gaaattgta 
      ttcgctaga acctcgccg caacagaag ttgatcatc 
      gtcgagggg tgccaacgg atgggcgct attgtcgcc 
      gtgacaggc gacggcgtg aacgactcg ccggctttg 
      aagaaggcg gacatcggt gtggccatg ggtatcgcg 
      ggttcggat gtgtccaag caagccgcc gacatgatc 
      ctgctggac gataacttc gcgtcgatc gtgacagga 
      gtggaggaa ggccgtttg atcttcgat aacttgaag 
      aaatctatt gcctacacc ttgacctca aacattccc 
      gaaatctcg cctttcctt gctttcatt ttgtgcgac 
      attcctttg cctctcggt accgtaaca attctgtgc 
      atcgatctt ggaactgac atggttcca gccatatct 
      ttggcgtac gaggaagca gaatctgat attatgaag 
      agaccaccc cgagaccca cagaatgat aaacttgtc 
      aatgatagg tgctgtttt ctacatttt ttttattct 
      aacaatgtg gtcgtttat tttgtattg attttttgg 
      actaactgt atatatttg cagtatatt tggggattt 
      ttaacacta ataacgggt tgcactgat gcctga
    mRNA sequence
    • >TC033838 RA
      caccgcgta tttaaaaaa ccgcacacc tgttttttt 
      gttctcaat gtttgccac attgtgcgg ccaggttgt 
      tgtaatgta atggctttg aagagcgag atgaaggaa 
      gagcacggc cgttctgac tcgtatcgg gtggcaact 
      attccgtcc agttatgat gacaacaaa acggctgat 
      ggtagacca aagtcacgg aggaaaaat aagaaggtc 
      aggaaagcg gacgattta gatgatttg aaacaagaa 
      ttggacatc gattatcat aaaatcacc ccagaagaa 
      ttatatcag agattccag acacatcca gaaaatggc 
      ctcagtcat gcgaaagcg aaagagaat ttggaacgg 
      gacggaccc aatgcactc acaccccca aagactacc 
      cccgaatgg gtgaaattt tgtaaaaat ctcttcggg 
      ggtttcgct ctcttattg tggatcggc gccatcctc 
      tgcttcata gcctattct attcaggct agcaccgtg 
      gaggaacca gccgatgat aatctttat cttggcatc 
      gtcttagct gccgttgtt atcgttaca ggtatattt 
      tcttattat caagaaagc aagagttcg aagattatg 
      gagtcgttc aaaaacatg gtcccccaa ttcgctaca 
      gtgatccgc gagggtgaa aagctgacc ctccgcgcg 
      gaggacctg gtactgggc gacgtggtc gaggtgaaa 
      ttcggtgac agaatccca gccgatatc cgaatcatc 
      gaatctcgc ggcttcaaa gtagacaac tcatccttg 
      acaggcgaa tccgaaccg cagtcccgc agtccggag 
      ttcactcac gagaaccct ctcgaaacg aaaaacttg 
      gcgttcttc tcgaccaac gccgtcgaa ggcactgcc 
      aaaggtgtt gtgattagt tgtggtgac aataccgtg 
      atgggtcgc atcgccggt ctcgcctcc ggtctggac 
      accggcgag acgcccatc gccaaagaa atccatcat 
      ttcattcac ctcattact ggcgtggct gttttcctc 
      ggagttacc ttcttcgta atcgccttc atcctcggc 
      taccactgg ctcgacgct gttattttc ctcatcggt 
      attatcgtg gcgaacgtg cccgagggg ctcctcgcc 
      accgtcacc gtgtgtctc accctcact gctaagagg 
      atggcttcc aagaactgc ctcgtgaag aatctcgag 
      gccgtagag accctcggc tccacaagc acgatctgc 
      tcggacaag accggaact ttgacccaa aaccggatg 
      acggtagca cacatgtgg ttcgacaat cagatcatt 
      gaagccgac accactgaa gaccagtcg ggagtccaa 
      tacgaccgc acaagtcca ggattcaaa gctttgtcg 
      cgcattgcc acactttgc aaccgggct gagttcaaa 
      ggggggcag aacgacgtc ccgatcctt aaacgcgaa 
      gtcaacgga gacgcctct gaagccgct ctcctcaaa 
      tgcatggaa ctggctctg ggcgacgtg atgtccatc 
      agacgcaag aacaagaaa gtttgcgaa attcccttc 
      aactcgacc aacaaatac caagtttcc atccacgag 
      aacgaggac gcgagcgat cctcgccat atccttgtg 
      atgaagggc gctcctgaa cgaatcctc gaacgctgc 
      agcacgatc ttcatctgc ggcaaggag aaagtcctg 
      gatgaggaa atgaaggaa gctttcaat aacgcctac 
      ttggagttg ggtggtttg ggcgagcgt gtgctcggc 
      ttctgcgat tttatgttg cccactgat aagtaccca 
      attgggtac aaattcaat tgcgatgac cccaacttc 
      ccgttggat ggtttgaga tttgttggc ttgatgtcc 
      atgattgat cctcccaga gctgcagtg cctgacgcc 
      gttgctaaa tgcagaagt gccggtatt aaggtcatt 
      atggtgacg ggagatcac ccgattacg gccaaggct 
      attgcaaag tcggttggg attatttcg gagggtaac 
      gaaacggtt gaagatatt gctcaacgg ttgaatatt 
      cctgtctcg gaagtcaac ccgagggaa gccaaagct 
      gccgttgtt cacggatct gatctcaga gacctatct 
      tccgatcaa ttagacgaa attttgaga taccacact 
      gaaattgta ttcgctaga acctcgccg caacagaag 
      ttgatcatc gtcgagggg tgccaacgg atgggcgct 
      attgtcgcc gtgacaggc gacggcgtg aacgactcg 
      ccggctttg aagaaggcg gacatcggt gtggccatg 
      ggtatcgcg ggttcggat gtgtccaag caagccgcc 
      gacatgatc ctgctggac gataacttc gcgtcgatc 
      gtgacagga gtggaggaa ggccgtttg atcttcgat 
      aacttgaag aaatctatt gcctacacc ttgacctca 
      aacattccc gaaatctcg cctttcctt gctttcatt 
      ttgtgcgac attcctttg cctctcggt accgtaaca 
      attctgtgc atcgatctt ggaactgac atggttcca 
      gccatatct ttggcgtac gaggaagca gaatctgat 
      attatgaag agaccaccc cgagaccca cagaatgat 
      aaacttgtc aatgatagg tgctgtttt ctacatttt 
      ttttattct aacaatgtg gtcgtttat tttgtattg 
      attttttgg actaactgt atatatttg cagtatatt 
      tggggattt ttaacacta ataacgggt tgcactgat 
      gcctgaggg gatataaag taactgcat gttttttgg 
      agtattgta tcattaaca atcatagag taatctata 
      acattggtt cgattaaca ttcgacaag ataaacagt 
      attgtcctg gttgagctt tttgtcttg tccatgggg 
      ttgtatcat ttgattttt gtgggttta ttgtataat 
      agctataat gaggacaaa aaacacaaa cacacattt 
      cccttctcc atgtatcac gcataatgc gtagtcgcc 
      ttgcttggc acagcttgc tcgacccct agtctctta 
      gagaggaac agatgtgcg cgtcatagt aatccgatt 
      gcattttcc cggcttttt tcatttttg ggaaagtgt 
      aatgcggtg gagttagta gaagcgttt gctacgcct 
      tgtcgtatt ttgtgggcg cagtgggcg tttctgggc 
      acgttgagt ctagttgag gcacatctg catatcgta 
      tagagttag gcaagtcaa ggagcagtg ctggcatgt 
      aagtgtgcc ctcccggtt cattgttct ccttccatg 
      tcccaagtt ttgttttga cgtgtattt gtttggccg 
      agctcaggt ccccgccat atccctggc atacgagga 
      ggcagagtc tgatataat gaagcgccg tcctagaaa 
      ccctttcag tgataaact cgtcaacga aaggtaata 
      ggaatgtca cgtgttctc tcctcttca tacctataa 
      tacgagtcg attggatca gaacgagat gtatcgtat 
      gccatgcgt acgcgataa cttctgctt ccccttata 
      atcctcccc tttgtgttt ttatccttc actcgagtt 
      tatttcgct tttttgctt cgccgcaat gttacgaag 
      caagcaatc gtttgattg acacaaaaa aacgattgg 
      ttgtttggt tgagatgct tttgtgcat agtgttcgt 
      acgaggggt aagtcgtta tttaatttc gagaagcac 
      atcattcat tctgcaccc cgcttatca gctgaggcc 
      ctcgtccct cctcccagc ccgcaactt ctttgacac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY