Status
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Gene information
General information
TC033785
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008839
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10658755..10662392
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033785
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09247

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033785 RA
      MITLNSTTS YFGYSILLQ KGNPPIAIV GAPRRKYSG 
      KPTGAVFKC NLEDLNCQE YTRVSRERG IYPIGKNGD 
      LLGSVLDGD EDRGGSFFT CAPRFIISP SFDTYFTNG 
      RCFHVKNSQ NIHENAELL LPLNDINQI GTDNFAPFY 
      DAAFGESGF SIRFLKEKN ELIIGAPGK YKWNGALLT 
      TSESHSTQI YPKRSNKFT SLDHYYGYD VAWGRFVKN 
      SSEIWYVSG APRGSGLKG SVIFYGTNP HDFEIKSVL 
      FGEEFGSYF GASLLVLHY AGGGDDILV GAPMHAGDS 
      WDEGCVYYY KKNGGGPYF HPPVKLSRG VSSSRFGTV 
      LADLGDIDL DSFPDCAIS APYENYGTG AVYLYFGSK 
      EGFSNDNYQ RIVPSNFAP FFPPMDVKG FGLGISKGV 
      DTKNKKHND FAVGAYKTG QVFYFQSYQ IVDITPDMT 
      PNIFTLHTS TTNFSVKYC LTFTYRNGK TKSVSFDTS 
      LVLVDSRVV NERNVTQSV TIEAKKQNC NHHTVRLKK 
      NHLNLEALT IRFLYKIQQ QNSWLEKIL SLPMSYDCG 
      NDNICQTNL TITAKQTDP KIIILGVNN VLRMNVSIN 
      NFGEPAYQT KLYLNVPPE ITLINLREC DFKNGSYEC 
      LVAQLLNET VEKHFLLDL ANIQPQWTF LPINFTITS 
      IGQDVYPSD NKLELNIPI TTHNNPRIT SVSQPDNIF 
      LNGTRRLNI THLFFVDNQ GPSPLNLTL EIYIPQVNL 
      NGSAVFEIE SVEESSNEI VSECIHLDK KPPLDLAEG 
      KNQANSTKV LSCLEPEIN CTSVICGTG YLQKSSQNL 
      VFSVKIFVH TDSIAQLIG DKNKLLLTT SAYFTNGTK 
      MFSTSTTTQ IYGISKNVK IPLWIYICS VVLGLVLLL 
      LLIFGLYKC NFFKRTYKE KIESERIFD DGVAQFEVG 
      NNLFCT
    CDS sequence
    • >TC033785 RA
      atgataaca cttaacagt acaacttct tatttcgga 
      tattcgatt cttcttcaa aaaggcaac cctccaata 
      gccattgtt ggcgcccca cgaagaaaa tattccggt 
      aaaccgaca ggtgctgta tttaaatgt aatctagaa 
      gatctaaat tgtcaagaa tacacaaga gtttcacga 
      gaacgaggg atttaccct atcggcaaa aacggggat 
      cttttagga tcagtgtta gatggagat gaggataga 
      ggaggaagt tttttcaca tgtgcgcct agatttatc 
      ataagtccc agctttgac acatatttt acaaatgga 
      agatgtttc catgttaaa aactcacaa aatattcat 
      gaaaacgcc gaactttta ctaccacta aacgacatt 
      aaccagatt ggcaccgat aattttgcc cctttctat 
      gacgctgca tttggagag tcaggtttc agtataaga 
      tttttaaaa gaaaaaaat gagctgata attggggcc 
      cctggtaag tataaatgg aacggtgcc ctgctcacc 
      actagtgag tctcattct acacaaata tacccaaaa 
      cggagcaac aagtttacc tctcttgac cattactat 
      gggtacgat gttgcttgg gggcggttt gtaaaaaat 
      tcaagtgag atatggtac gtgtctggt gcccccaga 
      gggtccggg cttaaaggc agtgtcatt ttttacgga 
      acaaacccg cacgacttc gaaattaag tcggtgctt 
      tttggggag gaattcgga tcgtacttt ggtgcttcc 
      ctactggtg cttcactat gcaggaggg ggtgacgat 
      attttagtc ggagcacca atgcatgct ggggattcc 
      tgggatgag gggtgtgtt tattattac aagaagaat 
      ggcggtgga ccctatttc caccctcct gtaaaactg 
      tcaagggga gtttcttcg tcacgattt gggacagtt 
      ttggctgat ttgggagac attgacctc gattctttc 
      ccagactgc gccatttcg gctccgtac gaaaattac 
      ggcacagga gccgtttat ttgtacttt ggcagtaaa 
      gagggattt tcgaacgat aactatcaa cgcattgtg 
      ccttcgaat tttgctccg ttctttccc ccaatggat 
      gtaaagggt tttgggctg ggaatatcc aaaggcgtt 
      gacacaaaa aataaaaaa cacaacgac tttgcagtc 
      ggggcgtat aaaaccggc caagttttt tatttccaa 
      tcttaccaa attgttgat atcacacct gatatgacc 
      ccaaacata tttactttg cacacttct accacgaat 
      ttttcggtc aaatattgc ctgacgttt acttacaga 
      aatggaaaa accaaaagt gtgtctttt gatacgagc 
      ttagttttg gtagatagc agggttgta aatgaacgc 
      aatgttaca caaagtgtt acaattgaa gcaaagaaa 
      caaaattgt aaccatcac accgttcgt ttaaaaaaa 
      aatcattta aacctggaa gctctcacc atccgtttt 
      ttgtataaa atacagcaa caaaatagt tggttagaa 
      aaaatatta agtctacca atgtcctac gactgcggg 
      aatgacaac atctgccaa actaactta acaatcacc 
      gcaaaacaa accgatcca aaaataata atactcgga 
      gtcaacaac gtactaaga atgaacgtt tcaattaac 
      aattttggg gaaccagca tatcaaacc aaactttac 
      ttaaacgtt ccaccggaa atcaccttg atcaatttg 
      agggaatgt gattttaaa aacggaagt tatgagtgt 
      ttggtggca cagttattg aatgaaacg gttgaaaaa 
      cacttcttg ctagatttg gccaacatt cagccccag 
      tggaccttc ttgcccatt aattttacc attactagc 
      attgggcaa gacgtctat ccttccgat aacaagctc 
      gagttgaat attcccatc acgactcat aataatcct 
      cgaattacc agtgtctca caacccgac aatattttc 
      ctaaatgga acacgacgt ttaaacatc actcatctc 
      ttctttgtg gacaatcaa ggcccgagt ccgcttaat 
      ctcactttg gaaatttac ataccacaa gtaaacctc 
      aacggaagt gctgttttc gaaatcgaa agtgttgaa 
      gagtctagt aacgagatt gtgagcgaa tgtattcat 
      ttggacaaa aaaccaccg cttgacttg gctgaagga 
      aaaaatcag gccaatagt acgaaagtt ttgagctgt 
      ttagagcca gaaattaat tgtacaagt gttatttgt 
      gggacggga tatttgcaa aaatcgagc caaaatctt 
      gttttttcg gtaaaaata tttgtccac actgattct 
      atagcacaa ttaattggt gacaaaaat aagctgctt 
      ttgacaacc agtgcttat ttcactaac gggacaaaa 
      atgttttcc acatcgaca acaacccaa atttatgga 
      atttcaaaa aatgtgaaa ataccgctg tggatttat 
      atttgttca gtcgtcttg ggacttgtc ttgttgcta 
      ctattaata ttcggttta tataaatgc aactttttc 
      aagcgcact tataaggaa aaaatagaa agtgagaga 
      attttcgat gatggtgtc gctcaattt gaggtgggt 
      aataatttg ttctgtaca taa
    mRNA sequence
    • >TC033785 RA
      ttagtcgct tcagttgtg acggtcatc tcttagata 
      acgacacac tcgtcgtat gtttgagaa atgttgaca 
      ccctttata atatttttt ttgttccgt tatttgtca 
      tgtgatatc aatataatc aattattcg tcaacaaaa 
      atgataaca cttaacagt acaacttct tatttcgga 
      tattcgatt cttcttcaa aaaggcaac cctccaata 
      gccattgtt ggcgcccca cgaagaaaa tattccggt 
      aaaccgaca ggtgctgta tttaaatgt aatctagaa 
      gatctaaat tgtcaagaa tacacaaga gtttcacga 
      gaacgaggg atttaccct atcggcaaa aacggggat 
      cttttagga tcagtgtta gatggagat gaggataga 
      ggaggaagt tttttcaca tgtgcgcct agatttatc 
      ataagtccc agctttgac acatatttt acaaatgga 
      agatgtttc catgttaaa aactcacaa aatattcat 
      gaaaacgcc gaactttta ctaccacta aacgacatt 
      aaccagatt ggcaccgat aattttgcc cctttctat 
      gacgctgca tttggagag tcaggtttc agtataaga 
      tttttaaaa gaaaaaaat gagctgata attggggcc 
      cctggtaag tataaatgg aacggtgcc ctgctcacc 
      actagtgag tctcattct acacaaata tacccaaaa 
      cggagcaac aagtttacc tctcttgac cattactat 
      gggtacgat gttgcttgg gggcggttt gtaaaaaat 
      tcaagtgag atatggtac gtgtctggt gcccccaga 
      gggtccggg cttaaaggc agtgtcatt ttttacgga 
      acaaacccg cacgacttc gaaattaag tcggtgctt 
      tttggggag gaattcgga tcgtacttt ggtgcttcc 
      ctactggtg cttcactat gcaggaggg ggtgacgat 
      attttagtc ggagcacca atgcatgct ggggattcc 
      tgggatgag gggtgtgtt tattattac aagaagaat 
      ggcggtgga ccctatttc caccctcct gtaaaactg 
      tcaagggga gtttcttcg tcacgattt gggacagtt 
      ttggctgat ttgggagac attgacctc gattctttc 
      ccagactgc gccatttcg gctccgtac gaaaattac 
      ggcacagga gccgtttat ttgtacttt ggcagtaaa 
      gagggattt tcgaacgat aactatcaa cgcattgtg 
      ccttcgaat tttgctccg ttctttccc ccaatggat 
      gtaaagggt tttgggctg ggaatatcc aaaggcgtt 
      gacacaaaa aataaaaaa cacaacgac tttgcagtc 
      ggggcgtat aaaaccggc caagttttt tatttccaa 
      tcttaccaa attgttgat atcacacct gatatgacc 
      ccaaacata tttactttg cacacttct accacgaat 
      ttttcggtc aaatattgc ctgacgttt acttacaga 
      aatggaaaa accaaaagt gtgtctttt gatacgagc 
      ttagttttg gtagatagc agggttgta aatgaacgc 
      aatgttaca caaagtgtt acaattgaa gcaaagaaa 
      caaaattgt aaccatcac accgttcgt ttaaaaaaa 
      aatcattta aacctggaa gctctcacc atccgtttt 
      ttgtataaa atacagcaa caaaatagt tggttagaa 
      aaaatatta agtctacca atgtcctac gactgcggg 
      aatgacaac atctgccaa actaactta acaatcacc 
      gcaaaacaa accgatcca aaaataata atactcgga 
      gtcaacaac gtactaaga atgaacgtt tcaattaac 
      aattttggg gaaccagca tatcaaacc aaactttac 
      ttaaacgtt ccaccggaa atcaccttg atcaatttg 
      agggaatgt gattttaaa aacggaagt tatgagtgt 
      ttggtggca cagttattg aatgaaacg gttgaaaaa 
      cacttcttg ctagatttg gccaacatt cagccccag 
      tggaccttc ttgcccatt aattttacc attactagc 
      attgggcaa gacgtctat ccttccgat aacaagctc 
      gagttgaat attcccatc acgactcat aataatcct 
      cgaattacc agtgtctca caacccgac aatattttc 
      ctaaatgga acacgacgt ttaaacatc actcatctc 
      ttctttgtg gacaatcaa ggcccgagt ccgcttaat 
      ctcactttg gaaatttac ataccacaa gtaaacctc 
      aacggaagt gctgttttc gaaatcgaa agtgttgaa 
      gagtctagt aacgagatt gtgagcgaa tgtattcat 
      ttggacaaa aaaccaccg cttgacttg gctgaagga 
      aaaaatcag gccaatagt acgaaagtt ttgagctgt 
      ttagagcca gaaattaat tgtacaagt gttatttgt 
      gggacggga tatttgcaa aaatcgagc caaaatctt 
      gttttttcg gtaaaaata tttgtccac actgattct 
      atagcacaa ttaattggt gacaaaaat aagctgctt 
      ttgacaacc agtgcttat ttcactaac gggacaaaa 
      atgttttcc acatcgaca acaacccaa atttatgga 
      atttcaaaa aatgtgaaa ataccgctg tggatttat 
      atttgttca gtcgtcttg ggacttgtc ttgttgcta 
      ctattaata ttcggttta tataaatgc aactttttc 
      aagcgcact tataaggaa aaaatagaa agtgagaga 
      attttcgat gatggtgtc gctcaattt gaggtgggt 
      aataatttg ttctgtaca taaaaaaat taatcggag 
      taatatttt tgccaggta ccgtctcat gatgaagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY