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Gene information
General information
TC009963 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11289599..11291718
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009963
    iBeetle screen iB_09244

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 prothorax musculature specific subset not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: potentially higher penetrance, but not all larvae are observable from the ventral side
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen segment number increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    posterior terminus potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009963 RA
      MPVDIKELT EGWLELESD PGLFSLLLE DFGVKGVQV 
      EEIYDIHKP LDGPVYGFI FLFRWMEER RSRRKIVEQ 
      TEVFVRDEE IVNDIFFAQ QMVPNSCAT HALVSVLLN 
      CADLHLGAT LERLRTHTR GMSPENKGW AIGNTPELA 
      KAHNSHAMP QAKRRLDKS SGVSTGRFT GEAFHFVSF 
      VPINGRLFE LDGLKPWPI DHGPWNEGD QWTDKFRAV 
      IADRLAISA GDEGSEIRF NLMAVVPDR RLAIHHKLK 
      MLKTNRQIV LDALKHLVK VKKDKKEET ASNNAMEED 
      PPQGNSQSQ SASEEGALT PKSSVTTLG PLDYATPLT 
      IQTSPAPST SGTDTSSEI GSAFNSPTQ TWNWGAQQQ 
      QPSPSSRDL KRFVVIRMN EQEEEQNSK KTVKKEGTE 
      QKSNGNGGV ARVRTSDGD TVVAEKKHP ELLEPHTFA 
      PQDLLALLR NLENEICMC EINLKDEND KQNKYKIDD 
      CRRTHNYDE FICTFLSML AEQGKLADL VEQHLQVPK 
      RPGTVPQPK AAKTAKKHE TGATKRKKG RTKVKRRR
    CDS sequence
    • >TC009963 RA
      atgccggtg gacataaag gaattaacc gaaggctgg 
      ctggagttg gaaagcgac ccaggcctg ttctccctt 
      ctcttggaa gacttcggc gtaaaaggc gtccaagtt 
      gaagaaatc tacgacatc cacaagccc ttagatggc 
      cccgtttac ggtttcatt ttcctgttc cgctggatg 
      gaggagcgg cgctcgcgg cgaaaaatc gtggagcaa 
      accgaagtg tttgttaga gatgaggaa atcgtaaac 
      gacatattt ttcgcgcag caaatggtc cccaacagt 
      tgcgcgacc cacgctctg gtttcagta ttgctaaac 
      tgtgccgac ttgcacttg ggggcgact ttagagcgg 
      ctacggacg cacactaga ggcatgtcg cctgagaat 
      aaaggctgg gcgattggg aacacccca gagctggcc 
      aaagcgcac aactcgcac gcgatgccg caagccaag 
      agacggcta gataagagt agcggggtt tcaactggg 
      cggtttaca ggagaggcg tttcatttc gttagtttt 
      gttccgata aacgggcgt ctgtttgaa ctggatggg 
      ctgaagccg tggcccatc gaccacggg ccgtggaac 
      gagggtgac caatggacg gataaattc cgggctgtt 
      atcgccgat agactggcg ataagtgcg ggagatgaa 
      ggaagcgaa atccggttt aatctaatg gcggtggtg 
      ccggaccgc cgacttgca attcaccac aagttaaaa 
      atgttgaaa acgaacaga cagatagtt ctggacgcg 
      ttaaaacat ctagtcaaa gttaaaaaa gacaaaaaa 
      gaagagact gcaagtaac aatgcaatg gaggaagat 
      ccccctcaa gggaattct cagagtcag tcagcgtct 
      gaagagggg gctttgacg cccaaaagc tcagttaca 
      acgctaggg ccgttagat tatgcgacg ccactcaca 
      attcaaaca tcaccagcc cccagcact tcaggaaca 
      gatacttcg tcggaaatt ggctcagcg ttcaattcg 
      ccaacgcag acctggaac tggggggcg caacagcaa 
      caaccaagt ccatcctcc agggatctg aagagattt 
      gttgtgatt cgaatgaac gagcaagag gaggagcaa 
      aatagcaag aaaactgtg aaaaaagaa ggcaccgaa 
      caaaaaagc aacgggaat gggggcgtc gcccgggtt 
      cgtacgagc gacggtgac acggttgtg gctgagaaa 
      aaacaccct gaacttttg gagcctcac acttttgcc 
      ccacaagac cttttagcg cttttgcgg aatttagaa 
      aacgagatt tgtatgtgt gagattaat ttaaaagat 
      gaaaatgat aagcagaat aaatataag attgatgac 
      tgcaggagg actcataat tatgacgaa tttatttgt 
      acgttttta tcaatgtta gcggagcaa ggaaaattg 
      gctgattta gttgaacaa catttgcaa gttcctaaa 
      aggcctggg actgtgccc caacctaaa gctgcgaaa 
      acggccaag aaacatgaa actggtgcg acgaaaagg 
      aagaagggg aggacgaaa gtaaagaga agaaggtga
    mRNA sequence
    • >TC009963 RA
      gggggcgat tatagcgtc ccacaatgc cggtggaca 
      taaaggaat taaccgaag gctggctgg agttggaaa 
      gcgacccag gcctgttct cccttctct tggaagact 
      tcggcgtaa aaggcgtcc aagttgaag aaatctacg 
      acatccaca agcccttag atggccccg tttacggtt 
      tcattttcc tgttccgct ggatggagg agcggcgct 
      cgcggcgaa aaatcgtgg agcaaaccg aagtgtttg 
      ttagagatg aggaaatcg taaacgaca tatttttcg 
      cgcagcaaa tggtcccca acagttgcg cgacccacg 
      ctctggttt cagtattgc taaactgtg ccgacttgc 
      acttggggg cgactttag agcggctac ggacgcaca 
      ctagaggca tgtcgcctg agaataaag gctgggcga 
      ttgggaaca ccccagagc tggccaaag cgcacaact 
      cgcacgcga tgccgcaag ccaagagac ggctagata 
      agagtagcg gggtttcaa ctgggcggt ttacaggag 
      aggcgtttc atttcgtta gttttgttc cgataaacg 
      ggcgtctgt ttgaactgg atgggctga agccgtggc 
      ccatcgacc acgggccgt ggaacgagg gtgaccaat 
      ggacggata aattccggg ctgttatcg ccgatagac 
      tggcgataa gtgcgggag atgaaggaa gcgaaatcc 
      ggtttaatc taatggcgg tggtgccgg accgccgac 
      ttgcaattc accacaagt taaaaatgt tgaaaacga 
      acagacaga tagttctgg acgcgttaa aacatctag 
      tcaaagtta aaaaagaca aaaaagaag agactgcaa 
      gtaacaatg caatggagg aagatcccc ctcaaggga 
      attctcaga gtcagtcag cgtctgaag agggggctt 
      tgacgccca aaagctcag ttacaacgc tagggccgt 
      tagattatg cgacgccac tcacaattc aaacatcac 
      cagccccca gcacttcag gaacagata cttcgtcgg 
      aaattggct cagcgttca attcgccaa cgcagacct 
      ggaactggg gggcgcaac agcaacaac caagtccat 
      cctccaggg atctgaaga gatttgttg tgattcgaa 
      tgaacgagc aagaggagg agcaaaata gcaagaaaa 
      ctgtgaaaa aagaaggca ccgaacaaa aaagcaacg 
      ggaatgggg gcgtcgccc gggttcgta cgagcgacg 
      gtgacacgg ttgtggctg agaaaaaac accctgaac 
      ttttggagc ctcacactt ttgccccac aagaccttt 
      tagcgcttt tgcggaatt tagaaaacg agatttgta 
      tgtgtgaga ttaatttaa aagatgaaa atgataagc 
      agaataaat ataagattg atgactgca ggaggactc 
      ataattatg acgaattta tttgtacgt ttttatcaa 
      tgttagcgg agcaaggaa aattggctg atttagttg 
      aacaacatt tgcaagttc ctaaaaggc ctgggactg 
      tgccccaac ctaaagctg cgaaaacgg ccaagaaac 
      atgaaactg gtgcgacga aaaggaaga aggggagga 
      cgaaagtaa agagaagaa ggtgaaaaa agaaatatt 
      tatttaaaa gatgtagga attaatata actttcata 
      atttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY