Status
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Gene information
General information
TC009950 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11421502..11425998
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009950
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09243

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009950 RA
      MDLGTYNLT GFYSYDNIS IAHSNVTAQ SFIEMVYVP 
      YEFVFNKAD GVMGLGLKT DENYDPFFY TLLKQKKIK 
      NPIFSIYLN RDRQSNRGG NVMLGFVDL KHVHKTKVG 
      KKIVYDNFT YLPVENYAY WQFQMDQVV VKVSKDNNV 
      VVCEKGCKA IVDTSSNAI VGPQADIAQ IHDAINANE 
      FFLHRYTVN CDTVNKLPK IDFMLGGTN FTLSGEDYT 
      VKMSYHSVT ICLSAFIPA NTIQEQDRW ILGGAFLAE 
      FYTVYDVQA RTIGFVKTN
    • >TC009950 RB
      MMSHCYLVT TFILLVLAK YGVADKKHT FSFEIRRQK 
      TPTEQYINH GHHFGRKFQ EVIEGPGPR DNDSIALYR 
      FLDDEFYGE IVIGHPGQK LNVAFDTTW SYSWVISSK 
      CSSIKTIGC YFHNKYDHN KSSEYKKDG RPFAMDLGT 
      YNLTGFYSY DNISIAHSN VTAQSFIEM VYVPYEFVF 
      NKADGVMGL GLKTDENYD PFFYTLLKQ KKIKNPIFS 
      IYLNRDRQS NRGGNVMLG FVDLKHVHK TKVGKKIVY 
      DNFTYLPVE NYAYWQFQM DQVVVKVSK DNNVVVCEK 
      GCKAIVDTS SNAIVGPQA DIAQIHDAI NANEFFLHR 
      YTVNCDTVN KLPKIDFML GGTNFTLSG EDYTVKMSY 
      HSVTICLSA FIPANTIQE QDRWILGGA FLAEFYTVY 
      DVQARTIGF VKTN
    • >TC009950 RC
      MMSHCYLVT TFILLVLAK YGVADKKHT FSFEIRRQK 
      TPTEQYINH GHHFGRKFQ EVIEGPGPR DNDSIALYR 
      FLDVS
    CDS sequence
    • >TC009950 RA
      atggatttg ggcacttac aacctaact gggttctat 
      tcttacgat aacatttct atagcccat tccaacgtc 
      actgcacaa tcgtttatc gaaatggtg tatgtcccg 
      tatgagttt gtgttcaac aaagcggac ggcgtaatg 
      ggtctaggc ttaaaaact gacgaaaat tacgatcct 
      tttttctac actttactg aaacagaag aaaataaag 
      aatccaatt ttttccatt tatttgaac agagatcgg 
      cagtcaaat cgtggaggt aatgttatg ttgggtttt 
      gttgacctt aagcacgtc cacaaaacc aaagtgggc 
      aaaaaaatc gtttatgac aatttcact tacttgccg 
      gtggaaaat tatgcttat tggcaattc caaatggac 
      caagttgtt gtaaaagtc agtaaagat aataatgtc 
      gttgtgtgt gaaaaaggt tgtaaagct attgtggac 
      actagctct aacgctatt gtaggccct caagccgac 
      atcgctcaa atacacgac gcaattaac gcaaatgag 
      tttttcttg caccgatat acggtgaat tgcgatact 
      gtaaataaa ttaccgaaa attgacttt atgttgggt 
      gggacaaat tttacactt tcgggagaa gactacacg 
      gttaaaatg tcttatcat tctgtgact atttgccta 
      tcagctttt attcctgcc aacacaatt caagaacaa 
      gatcgctgg attcttgga ggggcattt ttggccgaa 
      ttttataca gtttacgac gtacaagct aggaccatt 
      ggctttgtt aaaactaat tag
    • >TC009950 RB
      atgatgtct cattgttac ttagtcaca acttttatt 
      ttacttgtg ctggccaaa tacggtgtt gccgacaag 
      aaacatact tttagtttt gaaattcgc cggcaaaaa 
      acacccact gaacagtac atcaaccat ggacatcat 
      tttggtcgt aagttccag gaagttatt gaggggccg 
      gggccacgg gataacgac tccatcgct ttgtacagg 
      tttttggac gatgaattt tatggtgaa atagttatt 
      ggccatcct gggcaaaag ctgaacgtt gcgtttgat 
      acaacttgg agttactca tgggtgata tcctcgaag 
      tgcagttct atcaaaacg attggatgc tattttcac 
      aacaaatac gatcataac aaatcatct gaatataaa 
      aaggatggt cgtcctttt gcaatggat ttgggcact 
      tacaaccta actgggttc tattcttac gataacatt 
      tctatagcc cattccaac gtcactgca caatcgttt 
      atcgaaatg gtgtatgtc ccgtatgag tttgtgttc 
      aacaaagcg gacggcgta atgggtcta ggcttaaaa 
      actgacgaa aattacgat ccttttttc tacacttta 
      ctgaaacag aagaaaata aagaatcca attttttcc 
      atttatttg aacagagat cggcagtca aatcgtgga 
      ggtaatgtt atgttgggt tttgttgac cttaagcac 
      gtccacaaa accaaagtg ggcaaaaaa atcgtttat 
      gacaatttc acttacttg ccggtggaa aattatgct 
      tattggcaa ttccaaatg gaccaagtt gttgtaaaa 
      gtcagtaaa gataataat gtcgttgtg tgtgaaaaa 
      ggttgtaaa gctattgtg gacactagc tctaacgct 
      attgtaggc cctcaagcc gacatcgct caaatacac 
      gacgcaatt aacgcaaat gagtttttc ttgcaccga 
      tatacggtg aattgcgat actgtaaat aaattaccg 
      aaaattgac tttatgttg ggtgggaca aattttaca 
      ctttcggga gaagactac acggttaaa atgtcttat 
      cattctgtg actatttgc ctatcagct tttattcct 
      gccaacaca attcaagaa caagatcgc tggattctt 
      ggaggggca tttttggcc gaattttat acagtttac 
      gacgtacaa gctaggacc attggcttt gttaaaact 
      aattag
    • >TC009950 RC
      atgatgtct cattgttac ttagtcaca acttttatt 
      ttacttgtg ctggccaaa tacggtgtt gccgacaag 
      aaacatact tttagtttt gaaattcgc cggcaaaaa 
      acacccact gaacagtac atcaaccat ggacatcat 
      tttggtcgt aagttccag gaagttatt gaggggccg 
      gggccacgg gataacgac tccatcgct ttgtacagg 
      tttttggac gtgagttaa
    mRNA sequence
    • >TC009950 RA
      caggatgaa ttttatggt gaaatagtt attggccat 
      cctgggcaa aagctgaac gttgcgttt gatacaact 
      tggagttac tcatgggtg atatcctcg aagtgcagt 
      tctatcaaa acgattgga tgctatttt cacaacaaa 
      tacgatcat aacaaatca tctgaatat aaaaaggat 
      ggtcgtcct tttgcaatg gatttgggc acttacaac 
      ctaactggg ttctattct tacgataac atttctata 
      gcccattcc aacgtcact gcacaatcg tttatcgaa 
      atggtgtat gtcccgtat gagtttgtg ttcaacaaa 
      gcggacggc gtaatgggt ctaggctta aaaactgac 
      gaaaattac gatcctttt ttctacact ttactgaaa 
      cagaagaaa ataaagaat ccaattttt tccatttat 
      ttgaacaga gatcggcag tcaaatcgt ggaggtaat 
      gttatgttg ggttttgtt gaccttaag cacgtccac 
      aaaaccaaa gtgggcaaa aaaatcgtt tatgacaat 
      ttcacttac ttgccggtg gaaaattat gcttattgg 
      caattccaa atggaccaa gttgttgta aaagtcagt 
      aaagataat aatgtcgtt gtgtgtgaa aaaggttgt 
      aaagctatt gtggacact agctctaac gctattgta 
      ggccctcaa gccgacatc gctcaaata cacgacgca 
      attaacgca aatgagttt ttcttgcac cgatatacg 
      gtgaattgc gatactgta aataaatta ccgaaaatt 
      gactttatg ttgggtggg acaaatttt acactttcg 
      ggagaagac tacacggtt aaaatgtct tatcattct 
      gtgactatt tgcctatca gcttttatt cctgccaac 
      acaattcaa gaacaagat cgctggatt cttggaggg 
      gcatttttg gccgaattt tatacagtt tacgacgta 
      caagctagg accattggc tttgttaaa actaattag 
      ctttgctta acctgcctt gtaatcttt ttttgctct 
      ctagtgttt tgtattttg cttccaata aatgattaa 
      tgccttt
    • >TC009950 RB
      ggcttttgg tattcgatc gtgagttgt gtctaacgt 
      aaaacaata aaccggtgt ttggagcaa tttttgtgt 
      gaaaataat cgaaaatga tgtctcatt gttacttag 
      tcacaactt ttattttac ttgtgctgg ccaaatacg 
      gtgttgccg acaagaaac atactttta gttttgaaa 
      ttcgccggc aaaaaacac ccactgaac agtacatca 
      accatggac atcattttg gtcgtaagt tccaggaag 
      ttattgagg ggccggggc cacgggata acgactcca 
      tcgctttgt acaggtttt tggacgatg aattttatg 
      gtgaaatag ttattggcc atcctgggc aaaagctga 
      acgttgcgt ttgatacaa cttggagtt actcatggg 
      tgatatcct cgaagtgca gttctatca aaacgattg 
      gatgctatt ttcacaaca aatacgatc ataacaaat 
      catctgaat ataaaaagg atggtcgtc cttttgcaa 
      tggatttgg gcacttaca acctaactg ggttctatt 
      cttacgata acatttcta tagcccatt ccaacgtca 
      ctgcacaat cgtttatcg aaatggtgt atgtcccgt 
      atgagtttg tgttcaaca aagcggacg gcgtaatgg 
      gtctaggct taaaaactg acgaaaatt acgatcctt 
      ttttctaca ctttactga aacagaaga aaataaaga 
      atccaattt tttccattt atttgaaca gagatcggc 
      agtcaaatc gtggaggta atgttatgt tgggttttg 
      ttgacctta agcacgtcc acaaaacca aagtgggca 
      aaaaaatcg tttatgaca atttcactt acttgccgg 
      tggaaaatt atgcttatt ggcaattcc aaatggacc 
      aagttgttg taaaagtca gtaaagata ataatgtcg 
      ttgtgtgtg aaaaaggtt gtaaagcta ttgtggaca 
      ctagctcta acgctattg taggccctc aagccgaca 
      tcgctcaaa tacacgacg caattaacg caaatgagt 
      ttttcttgc accgatata cggtgaatt gcgatactg 
      taaataaat taccgaaaa ttgacttta tgttgggtg 
      ggacaaatt ttacacttt cgggagaag actacacgg 
      ttaaaatgt cttatcatt ctgtgacta tttgcctat 
      cagctttta ttcctgcca acacaattc aagaacaag 
      atcgctgga ttcttggag gggcatttt tggccgaat 
      tttatacag tttacgacg tacaagcta ggaccattg 
      gctttgtta aaactaatt agctttgct taacctgcc 
      ttgtaatct ttttttgct ctctagtgt tttgtattt 
      tgcttccaa taaatgatt aatgccttt
    • >TC009950 RC
      ggcttttgg tattcgatc gtgagttgt gtctaacgt 
      aaaacaata aaccggtgt ttggagcaa tttttgtgt 
      gaaaataat cgaaaatga tgtctcatt gttacttag 
      tcacaactt ttattttac ttgtgctgg ccaaatacg 
      gtgttgccg acaagaaac atactttta gttttgaaa 
      ttcgccggc aaaaaacac ccactgaac agtacatca 
      accatggac atcattttg gtcgtaagt tccaggaag 
      ttattgagg ggccggggc cacgggata acgactcca 
      tcgctttgt acaggtttt tggacgtga gttaaataa 
      ttttgcata acaaaaccc ataaaatgt agtagcgga 
      gtgattttg cagtgttta ttcacgatt tttctaggg 
      aaattcgaa actatatgc gtccatcaa agcttcaat 
      tgtatttat ttgtgtatt aatgaatta atgcaatat 
      ttatttaat tttttttta aataagacc tgcttctaa 
      aaatatgtt tggtaaatg cttgactta tcaaattat 
      agagacgac tggcaggcg aaaataaaa gctgcagcc 
      gtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY