Status
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Gene information
General information
TC008903 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11511562..11515748
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008903
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09241

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008903 RA
      MVTKATTKE AVRREISER LKHLTSEEK 
      QTQSKIVFE KLLNLPSFQ KSKRVSTYL 
      SIDNEIDTE PIVRKIFET GKECFVPRY 
      SKAGMEMVK IHSYQDWET LPVTSWGIK 
      QPGLKEQRE DALKTGGLD FVVVPGVGF 
      TAKGLRLGH GGGYYDRYM ADLRKNQDV 
      PPALVGLAF KEQICDDLP VTELDVQID 
      LVLFPE
    CDS sequence
    • >TC008903 RA
      atggtgaca aaagcaacc acgaaagaa gccgtccgt 
      cgtgaaatc agcgaacgg ctcaagcac ctcacttct 
      gaagaaaag caaactcag tcaaaaata gttttcgaa 
      aagttatta aatttacct tcgttccaa aagagcaaa 
      cgtgtgtca acatactta agtattgac aatgaaatc 
      gatacagag ccaattgta cgaaaaata ttcgaaacc 
      gggaaagaa tgttttgtt ccgagatac agcaaagct 
      gggatggaa atggtcaaa atacactcg taccaggac 
      tgggagacc ctgccggtg accagctgg gggataaaa 
      cagccgggt ttgaaggag caaagagag gatgcttta 
      aagacaggc ggtttggat tttgttgtt gttccgggg 
      gtcggcttt accgccaaa ggactcagg ctagggcat 
      gggggcggt tattacgac agatatatg gcagacttg 
      aggaaaaat caggatgtt ccgccggct cttgtaggt 
      ttggcgttc aaggaacaa atctgtgac gatctaccg 
      gtcacagag ctggacgta cagatcgac ctggttctg 
      tttcccgaa taa
    mRNA sequence
    • >TC008903 RA
      tacgattga agcacaatt tcattaaag cttggtccg 
      ccaaggtgc tcatccctt attcattca tcgttgtgt 
      aaggttccg gtgtggttt aatacatag ataaagtaa 
      atcaggcag aatatgaaa tttgggggt cgccaaaag 
      gttttttct gccttatca ctgtacgcg acaatggtg 
      acaaaagca accacgaaa gaagccgtc cgtcgtgaa 
      atcagcgaa cggctcaag cacctcact tctgaagaa 
      aagcaaact cagtcaaaa atagttttc gaaaagtta 
      ttaaattta ccttcgttc caaaagagc aaacgtgtg 
      tcaacatac ttaagtatt gacaatgaa atcgataca 
      gagccaatt gtacgaaaa atattcgaa accgggaaa 
      gaatgtttt gttccgaga tacagcaaa gctgggatg 
      gaaatggtc aaaatacac tcgtaccag gactgggag 
      accctgccg gtgaccagc tgggggata aaacagccg 
      ggtttgaag gagcaaaga gaggatgct ttaaagaca 
      ggcggtttg gattttgtt gttgttccg ggggtcggc 
      tttaccgcc aaaggactc aggctaggg catgggggc 
      ggttattac gacagatat atggcagac ttgaggaaa 
      aatcaggat gttccgccg gctcttgta ggtttggcg 
      ttcaaggaa caaatctgt gacgatcta ccggtcaca 
      gagctggac gtacagatc gacctggtt ctgtttccc 
      gaataaaac acgtcttct gttgtatat ttttatata 
      catgtacat ttaatataa aatataatt ccctgtacc 
      aagccaaca ctatgatac aaatatata aatacaatt 
      aataaaatc aagtccttg tcgttaaga acccttcaa 
      cacttataa caaattcac taactttaa agtgacaat 
      actgaagca catttatta ggccagatt tttaagtgc 
      aatactatt ggtctacta tcctattgt catcacaag 
      atcaggtca aaaatcatg gaataagtc cctacttga 
      gattcaaat catttggcg agatttatc ataatatca 
      acaaacaca accttatgt aataagttc cttgatact 
      tcgaccctg agagtcttt cacaaatct gcgttttgg 
      aagcgacag tcaggtaga agtttgtaa gatctaagg 
      ccactattt ggctgagaa agcagaatc gaagtacaa 
      ggtggtgaa gtcgtcaag gctgcgatt ttaactgtt 
      ttagaatta tttgactgt ggtagcagc gctgacgac 
      tcaaagaaa attggacaa gtttcgttg aatggaagt 
      tgtggtaaa aactagatg tgctctgca atctctatt 
      tacaacacg ctagtgtga actagaaca aagcaccta 
      aaaattaat tgtccaatc tccttcaaa ccaaatttt 
      cagtaacag tatgtaatg acacttaga cttacttac 
      caatattga aataaatac ataattata cacaattag 
      atcgagtac gaatattaa agctttttt atacttact 
      actgtgcac tttccttat ttacgtcat accaactta 
      aatgctaaa atattgagt atttttaca actaggtaa 
      atgattcaa accagcact ttctaacac aaaatttcg 
      gcgtttgaa tggtaccga aagtactgg cttgcacaa 
      aaacgataa aatcaagtt ataaaataa taataaaaa 
      acaatataa aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY