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Gene information
General information
TC016355 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6939527..6944833
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016355
    iBeetle screen iB_09229

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016355 RA
      MECGCAAPY IGPSLANTC GGGAFVLFM SLLDTFIRN 
      KCDLSEICQ RVVPKTQPD IEYDFVVIG GGSGGATAA 
      GRLSEVPEW KVLLIEAGG DEPPGSQVP SMVISYHGD 
      PHMDWNYKT EPEQQACLG FPEKRCSWP RGKVLGGCS 
      VINGMMYMR GHPKDYDNW ATMGNTGWG YQDVLPVFK 
      KSEDNLQIG TLVDAAYHG TGGPMTTSR FPHHPELAE 
      DVMQAAKEL GYPVSDDLN GRQYHGFTI AQSSVRNGS 
      RLSSARAFL RPGRDRPNL HVMLNSTAT KILINSSNN 
      QKTVSGVQF LYNNKLHTV RVKREVVVS AGAINSPQI 
      LLLSGIGPK EELDKVNIQ QVHQLPGVG KNLHNHVTF 
      YMTYEMKKQ KAVHDLDWA HALDYILNR RGPMSSTGM 
      SQVTARINS KFADPSGTH PDLQIFFAG YLANCAASG 
      EVRAAKDPE HPDAPRHLT ISPVVLHPK SRGHIGLKS 
      NNPLDPPLM YANYLSEPE DVATLVEGI RVTQRLANT 
      SVLQNKYGL TLMRDEYGD CEKKFTYDS DDFWQCAAR 
      YYTGPENHQ AGSCKMGPA SDPMAVVDP KLQVYGIEG 
      LRVMDASIM PALVSGNTH ATIVMIADK GVEYIKQKW 
      LRGGTIANR FGGGTSQSN QNAPHFYPS ASSNYPNYP 
      KQHAPYHYQ QGVKSTGFR HNEQFHRNH PQMPNPFMS 
      TPRPQRAYN QQGFNQGYQ GYQHNYPDY QQDQEYNNF 
      NAY
    CDS sequence
    • >TC016355 RA
      atggagtgt ggttgtgcg gcgccctac atcggtccg 
      tccctggcc aacacttgc gggggcggc gcttttgtc 
      ctcttcatg agccttttg gacactttc atacggaac 
      aaatgcgac ttgtccgaa atctgccaa agagtcgtt 
      ccgaaaacg cagcctgac atagagtac gactttgtg 
      gtgatcggg ggcggcagc ggcggtgcg acggcggca 
      ggaagactc tcggaagtc cccgagtgg aaggttttg 
      ttgattgaa gccggcggt gacgaacca ccagggtcc 
      caagtgcct tcgatggtt ataagctac catggagac 
      ccacacatg gactggaac tacaagacg gaaccggaa 
      caacaggca tgtcttgga tttccggaa aagaggtgt 
      tcctggcct agagggaag gttttgggt gggtgcagt 
      gtcatcaat gggatgatg tacatgcgg gggcatcct 
      aaggattac gataattgg gccacgatg ggcaatacg 
      ggatggggg taccaagac gtactccca gttttcaaa 
      aaatcagag gataatctg caaattggt actcttgtc 
      gatgctgct tatcatggt actggtggg ccaatgacc 
      acgtcccgg tttccgcat catcccgaa ttggcagaa 
      gacgttatg caggcggcg aaggagttg ggatatcct 
      gtcagtgac gatttgaat gggcgacag taccatggg 
      tttactata gcccagtcg agtgtgagg aacggatct 
      cgtctaagc agcgcccgg gcattcctc cgtccgggc 
      cgcgaccgc cccaacctc cacgtcatg ctgaactcc 
      accgcgacg aaaattcta ataaacagc agcaacaac 
      cagaagaca gtttcaggc gtccaattc ctctacaac 
      aataaatta cacacagtc agagtaaag cgggaagtc 
      gtcgtttcc gcaggagcc atcaactcg ccgcaaatt 
      ctcctctta tccggcatc ggccccaaa gaagaatta 
      gacaaagtt aacattcaa caagtccac caactcccc 
      ggcgtcggt aaaaacctc cacaaccac gtcactttc 
      tacatgacg tacgaaatg aaaaagcag aaagctgtc 
      catgacctg gactgggcc cacgctctg gattacatc 
      ctcaatcgg cgagggccg atgtcttcg acgggcatg 
      tcacaagtc acggcgagg atcaactcg aaattcgct 
      gatccttcg ggaactcat cccgatttg cagatcttc 
      tttgccggg tatttggcg aactgtgcc gcctccggg 
      gaagtgcgg gcggctaag gatccagaa catccagac 
      gctccgaga catctgacg atctcgccg gtggtgctg 
      catccaaag agtaggggg cacatcgga ttgaagtcg 
      aacaatccg ttagatccc ccgctcatg tatgccaac 
      tatttgagc gaaccggaa gacgtcgcg acgctagtc 
      gagggaatc agagtgacg cagagattg gccaatacg 
      agcgtcctt cagaacaag tacggcctg actttgatg 
      cgcgacgaa tacggggat tgcgagaaa aaatttacg 
      tatgattcg gatgacttt tggcaatgc gcagctcgc 
      tactatacc gggccggag aaccaccaa gcgggctct 
      tgtaagatg ggtccggcc tccgacccc atggctgtg 
      gtcgacccc aaactccaa gtctacggt attgagggg 
      ctccgtgtt atggatgcc tcgatcatg ccagcccta 
      gtctctgga aacacgcac gctaccatt gttatgatt 
      gctgataaa ggcgtcgag tacataaag caaaaatgg 
      ttgcgtggt ggtactatt gcgaacagg tttgggggt 
      ggaaccagt cagagtaat caaaacgca cctcacttt 
      tacccatcg gctagttca aattatccc aattaccct 
      aaacaacac gctccgtat cattaccaa cagggagta 
      aaaagtacg ggatttagg cataatgag caattccac 
      aggaatcat ccccaaatg cctaatcct ttcatgtcg 
      acgcctagg ccacaaagg gcgtataat cagcaggga 
      tttaatcaa gggtatcaa ggataccaa cataattat 
      cccgactac caacaggat caagaatat aacaatttc 
      aatgcatat taa
    mRNA sequence
    • >TC016355 RA
      agtccggcg ataaatcgt gcgcatccg actggataa 
      tccgagcta agtaagtcc gattgtgaa gacgggatg 
      ccccgctga cgtaacaac gcatctcga taacagttc 
      agagtcgcg ggtttgacc aaataacag ttccaccgg 
      ttatttaac gttaatccg aacctgttt tttactcag 
      tcaatagca agcgatgga gtgtggttg tgcggcgcc 
      ctacatcgg tccgtccct ggccaacac ttgcggggg 
      cggcgcttt tgtcctctt catgagcct tttggacac 
      tttcatacg gaacaaatg cgacttgtc cgaaatctg 
      ccaaagagt cgttccgaa aacgcagcc tgacataga 
      gtacgactt tgtggtgat cgggggcgg cagcggcgg 
      tgcgacggc ggcaggaag actctcgga agtccccga 
      gtggaaggt tttgttgat tgaagccgg cggtgacga 
      accaccagg gtcccaagt gccttcgat ggttataag 
      ctaccatgg agacccaca catggactg gaactacaa 
      gacggaacc ggaacaaca ggcatgtct tggatttcc 
      ggaaaagag gtgttcctg gcctagagg gaaggtttt 
      gggtgggtg cagtgtcat caatgggat gatgtacat 
      gcgggggca tcctaagga ttacgataa ttgggccac 
      gatgggcaa tacgggatg ggggtacca agacgtact 
      cccagtttt caaaaaatc agaggataa tctgcaaat 
      tggtactct tgtcgatgc tgcttatca tggtactgg 
      tgggccaat gaccacgtc ccggtttcc gcatcatcc 
      cgaattggc agaagacgt tatgcaggc ggcgaagga 
      gttgggata tcctgtcag tgacgattt gaatgggcg 
      acagtacca tgggtttac tatagccca gtcgagtgt 
      gaggaacgg atctcgtct aagcagcgc ccgggcatt 
      cctccgtcc gggccgcga ccgccccaa cctccacgt 
      catgctgaa ctccaccgc gacgaaaat tctaataaa 
      cagcagcaa caaccagaa gacagtttc aggcgtcca 
      attcctcta caacaataa attacacac agtcagagt 
      aaagcggga agtcgtcgt ttccgcagg agccatcaa 
      ctcgccgca aattctcct cttatccgg catcggccc 
      caaagaaga attagacaa agttaacat tcaacaagt 
      ccaccaact ccccggcgt cggtaaaaa cctccacaa 
      ccacgtcac tttctacat gacgtacga aatgaaaaa 
      gcagaaagc tgtccatga cctggactg ggcccacgc 
      tctggatta catcctcaa tcggcgagg gccgatgtc 
      ttcgacggg catgtcaca agtcacggc gaggatcaa 
      ctcgaaatt cgctgatcc ttcgggaac tcatcccga 
      tttgcagat cttctttgc cgggtattt ggcgaactg 
      tgccgcctc cggggaagt gcgggcggc taaggatcc 
      agaacatcc agacgctcc gagacatct gacgatctc 
      gccggtggt gctgcatcc aaagagtag ggggcacat 
      cggattgaa gtcgaacaa tccgttaga tcccccgct 
      catgtatgc caactattt gagcgaacc ggaagacgt 
      cgcgacgct agtcgaggg aatcagagt gacgcagag 
      attggccaa tacgagcgt ccttcagaa caagtacgg 
      cctgacttt gatgcgcga cgaatacgg ggattgcga 
      gaaaaaatt tacgtatga ttcggatga cttttggca 
      atgcgcagc tcgctacta taccgggcc ggagaacca 
      ccaagcggg ctcttgtaa gatgggtcc ggcctccga 
      ccccatggc tgtggtcga ccccaaact ccaagtcta 
      cggtattga ggggctccg tgttatgga tgcctcgat 
      catgccagc cctagtctc tggaaacac gcacgctac 
      cattgttat gattgctga taaaggcgt cgagtacat 
      aaagcaaaa atggttgcg tggtggtac tattgcgaa 
      caggtttgg gggtggaac cagtcagag taatcaaaa 
      cgcacctca cttttaccc atcggctag ttcaaatta 
      tcccaatta ccctaaaca acacgctcc gtatcatta 
      ccaacaggg agtaaaaag tacgggatt taggcataa 
      tgagcaatt ccacaggaa tcatcccca aatgcctaa 
      tcctttcat gtcgacgcc taggccaca aagggcgta 
      taatcagca gggatttaa tcaagggta tcaaggata 
      ccaacataa ttatcccga ctaccaaca ggatcaaga 
      atataacaa tttcaatgc atattaata gttgtagtt 
      aggaaacac ctatttctt ggtgtttta aagttttag 
      cttgttagg attttttta tttatttgt agaagccac 
      aaactgaga gtaagagaa cgggaaagt agaagcaac 
      aaatttttt atttttatt atgacaatt taaaaataa 
      atttcttat caacaacaa aattgtaca tttttttat 
      actttatta gtattactt acaacacaa ttaaatatg 
      aactaaaac a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY