Status
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Gene information
General information
TC013301 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6527932..6537112
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013301
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09227

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013301 RA
      MMESSCSEL QKVLVDVLS DLKYMSTQV IKKSDKLLN 
      DVESLNPSS VEPLAQNFK TLIERININ VGEFVNTFN 
      SAFETYKKA DEAEKSKTA SSLKTREDL PEVKAVTQE 
      TDIDLVCDI DSNAQLPGT SGTLDIPTV DIQDDKSDD 
      GSETDCDEP TLRNFDIPL ADGSVDDVA GELKKALDE 
      LTSQDETKH EIRHHFETA SFDFDDVDE QLTVAIEKA 
      KDSPGRGQE DNHELLDLL NKAVDDSDV DLITKGDQA 
      LQILEEDMS LSSCQDSFS DDSLSDDDN SDNEVNRKN 
      EDNVEDFCS SVAAEDCQN FSLKLRRID SSDGEIDRL 
      CNTDTLKRK CKKITARHR KKKYRRLIP LDSSSSSSS 
      SEDENSSDG SVTPDLIID EQRPSSPVF GDDAENEAL 
      AALADNLVD RLDSGESSS DSDTTEEEE PAKKKRKMT 
      PENMDESKN VKTTTEDKD TSSSSLDEE TIPKRKSWK 
      NDPLLRSSS SDSDSDADS LVFHVRDKR TKDNTDLNE 
      NVVKVNNNS DKRNVVEIE NLDSDQESI SENKNEIKV 
      ANNNFETMI ITDSSDSSS DSDDVLIVE DETGGTKGR 
      RNIRAILSD DELNELTQQ AKNEENERI MRLAEHSKN 
      IESIQSQHE DFILDFDVK TGEPLVTVD HKLAKMLKP 
      HQKSGIQFM WEACYESVE ILKTKPGSG CILAHCMGL 
      GKTLQVITL IHTLFTHPV TKTKHVLVV CPLSTVINW 
      KNEFKKAFK QLDNPPDIY PYWIIKGDI SEKINIIHT 
      WRQTGGVLI LGYDAYQII TNEKTALRI TAVEKQRAL 
      EALVDPGPD LIVCDEGHQ LKNGKTLKT QALMKVKTK 
      RRIVLTGTP LQNNLKEYY FMVQFVKPH LLGTYLEYT 
      NRFASPIMN GQFHDSTPG DIKLMKKRT HVLTKMLKN 
      TIHRVEGSV LSTYLPEIT DYTIFIKLT PLQIDLYTR 
      YIDLVTGQA SNLSSTFFH DVRMTNFCN LHPYALHLH 
      YSKPTLKRK AKTAGLIEN LEGDDNNVE HIRADWYKN 
      LLPADVSTN INYSTKIKL ILDIISECM RNNEKVLIF 
      GQYLVELDI VEHFLKQFR NWRPNVDYY RMDGDTSVE 
      NRDILCKKF NSNPTSKVF LLTHKVGGL GLNLTGANR 
      VILIGSNHN PSHDSQSLY RVYRFGQER KCYVYRLVS 
      LGTMEEKIY HRCVLKLSI SGTVVDKLH FDRRYKTTD 
      LKEMYKYDF DKYNSERPI PAVPSDMLM ALLLKNCPD 
      IFGYHLHNA LLENRPEEE LTDEDKKLA WDEFNNLAE 
      AARTNAPNP PNIPNLVLN PMSTNTRGF VPKTQGPST 
      PNNPFVNLL LGRIGLENG ELKKIMSDR FKMTMANKT 
      PTTGEFNYA LNKFI
    CDS sequence
    • >TC013301 RA
      atgatggaa agttcgtgt tcggaactc caaaaggtc 
      ctagttgac gtgttgagt gacctgaag tacatgtcg 
      actcaagtt ataaaaaag tcggataaa ttattgaac 
      gacgtagaa tcacttaat ccgagttcc gttgagcct 
      ttagcgcaa aattttaaa actctcatc gagcggata 
      aatataaat gtgggcgag tttgtaaac acattcaat 
      tcggccttt gaaacgtac aaaaaagca gacgaagcg 
      gagaaatcc aaaactgcg tctagtctt aagactcgg 
      gaggatctt cctgaagta aaagccgta acccaagag 
      actgacata gatttagtg tgtgatatt gactcaaat 
      gcacaattg ccaggaact tccggtact ctagacatt 
      cctactgta gatatccaa gatgacaaa agtgatgat 
      ggtagtgag actgattgt gacgagccc acattgcga 
      aattttgat attccttta gctgatggc tccgtagat 
      gacgtagcc ggagaatta aaaaaggct ttagatgaa 
      ctgactagc caagacgaa acaaaacac gaaattagg 
      catcatttt gagactgcg agttttgat tttgacgat 
      gtagatgaa cagttgact gttgcaata gaaaaagcc 
      aaagatagc ccaggtcgc ggacaagaa gacaatcat 
      gaacttttg gatttgttg aataaagca gtcgacgac 
      tctgacgtg gatttgata actaagggc gatcaagcc 
      ttacagatt ttagaagag gatatgtca ttatcaagt 
      tgtcaggat tcattctcg gatgattct ttgtctgat 
      gatgataat tccgataat gaagtaaac cgtaaaaac 
      gaagataat gttgaagac ttttgctct tccgtagct 
      gcagaagac tgccaaaat ttttctcta aaattacga 
      cgcattgat agcagtgat ggagaaata gatagatta 
      tgtaacacg gacacactc aaacgaaaa tgtaaaaaa 
      ataacagca cgacatagg aaaaagaaa tacaggcga 
      ttaatacct ttggacagt tcttcgtcg tcatcatcg 
      tctgaggat gaaaattcc tcagatggt tctgtaacg 
      cctgatctc attatagac gaacaacgc ccttcttca 
      cctgttttt ggtgatgat gcggaaaat gaggcactt 
      gcggctttg gccgataat ttagtggac agattggac 
      tcaggtgaa tcaagttca gattcggac actactgag 
      gaagaggag ccagctaag aagaaaagg aaaatgacg 
      cctgaaaat atggatgaa agtaaaaat gtaaagact 
      acaaccgaa gacaaggat acgagtagt tcgagtttg 
      gacgaagaa acgattcca aaacgcaaa agctggaaa 
      aacgatcct ttgcttcgc agttcgagc tcagacagc 
      gattcggat gcagattcg ttggtattc catgtgaga 
      gataaaaga acaaaagat aacactgat ctgaacgaa 
      aacgttgtc aaagttaat aataacagc gacaagaga 
      aatgtcgtg gaaattgaa aatcttgat tcagatcag 
      gaaagtatt agtgaaaat aaaaatgaa attaaggta 
      gcaaataac aatttcgaa actatgatt ataactgat 
      tcgtctgat tcaagttct gacagtgac gatgttctt 
      atcgttgag gatgaaacg ggtggaacg aaagggcgc 
      agaaacatc agggctatt ttaagtgac gatgaactt 
      aacgaatta actcagcaa gccaaaaat gaagaaaat 
      gagagaatt atgcgactc gccgagcat tccaaaaat 
      atcgagtca atacaatcc caacacgaa gactttata 
      ttagatttt gatgtaaag actggcgaa cctctagtc 
      accgtggac cacaaactc gcaaaaatg ttgaaacct 
      catcaaaaa agcggtatt caattcatg tgggaagca 
      tgttacgaa tcagttgaa attttaaaa accaagcca 
      ggatcgggc tgcattcta gcccattgc atgggctta 
      ggaaaaacc ctccaagtc atcacttta atccacacc 
      ctcttcacg catcctgtc acaaaaaca aaacatgtt 
      ctggtggtg tgtccgctg tctactgtg atcaattgg 
      aagaacgag tttaagaaa gccttcaaa caattggat 
      aatccgccc gatatttat ccttattgg attattaaa 
      ggtgatatt agtgagaaa ataaatatt atacatacg 
      tggcgccaa actggtggt gttttaatc ctgggttac 
      gacgcttat caaattata accaacgag aaaacagct 
      ttaagaata acagctgta gaaaagcaa cgggctttg 
      gaagctctc gttgatcca ggacctgat ttgattgtt 
      tgcgacgaa ggccaccaa cttaaaaat ggtaaaact 
      ctaaagacg caagcttta atgaaggtg aaaacgaaa 
      cgaaggatt gttctcaca gggacgcca ctacaaaat 
      aatctgaaa gaatattat tttatggtc cagtttgtt 
      aagccccat cttttggga acgtactta gagtacacg 
      aacaggttt gctagtcca attatgaat ggacagttt 
      catgactcg acacctgga gatatcaaa ttgatgaag 
      aagaggaca catgttctc accaaaatg ctcaaaaac 
      acaattcat agagtggaa ggtagtgtc ttgtcaacg 
      tatctgcca gaaattaca gactatacg atattcata 
      aaattaact ccattgcaa attgatctt tacacccgt 
      tacatagat ttggtgact ggacaggcc tccaatctg 
      agttcaaca ttcttccat gacgtgaga atgacaaat 
      ttctgcaat ttacatccc tacgctctt cacttacat 
      tacagcaaa ccaacgttg aaaagaaag gcgaaaact 
      gctggcctg attgaaaat ctcgaaggt gatgacaat 
      aatgtggag catatcaga gcagactgg tacaaaaat 
      ctgctacca gcggatgtt tccacaaat ataaattac 
      agtacaaag atcaaactc attttggac ataattagc 
      gaatgcatg cgaaataat gagaaagtt ttgatattt 
      ggacaatat ttggtcgaa cttgacatc gttgagcat 
      ttcctgaaa cagttcagg aactggaga cccaatgta 
      gactactac agaatggat ggcgacacg tctgtggaa 
      aatcgcgac atactgtgc aaaaaattc aattcgaat 
      cccacctct aaagtattt ctcctcaca cataaagtg 
      ggtggactg ggcttgaat ttgacaggt gcaaatcga 
      gtgattctg attggttcg aatcacaat ccctcccac 
      gacagtcag agtttatac agagtgtat cggttcggc 
      caagagagg aaatgttac gtttatcga cttgtatca 
      ctcgggaca atggaggag aaaatttac cacagatgc 
      gtcttgaaa ttgtctata tctggcacc gttgtcgat 
      aagttgcat ttcgatcgc cgttacaaa acgaccgat 
      ttgaaagaa atgtacaaa tatgatttc gataagtac 
      aatagcgaa agaccaata ccagcggtg ccaagtgat 
      atgttgatg gcactgctg ttgaaaaat tgtcccgat 
      atcttcggt taccactta cataatgcc ttacttgaa 
      aaccggcca gaagaggaa ctgaccgat gaggacaaa 
      aagttggct tgggacgaa ttcaacaat ttagcagag 
      gcagcaagg acaaatgca cccaatccg cccaacatt 
      ccaaaccta gtactcaat cccatgagt accaacaca 
      aggggtttt gtaccaaag actcaaggg ccctctacc 
      ccaaataac ccgtttgtg aatttgctt cttggtcgt 
      attggactt gaaaacggc gaattaaaa aagatcatg 
      tcagaccga tttaaaatg acaatggct aataaaaca 
      cctactact ggcgaattt aattacgca ttaaataag 
      tttatttaa
    mRNA sequence
    • >TC013301 RA
      aggggtggt tttcagatt atttttgtt attaatttt 
      ttctgcaaa acgttatga aatcagagg atttttggc 
      tgtagtgta gtgataacg tgcgtgaaa aattgttac 
      tgatgaaaa aattgactc aaatgtagt gaagtgact 
      tcataacct tacatttgc tcttccaat attattgtt 
      atttgagtt attactaaa agtatatta tgatggaaa 
      gttcgtgtt cggaactcc aaaaggtcc tagttgacg 
      tgttgagtg acctgaagt acatgtcga ctcaagtta 
      taaaaaagt cggataaat tattgaacg acgtagaat 
      cacttaatc cgagttccg ttgagcctt tagcgcaaa 
      attttaaaa ctctcatcg agcggataa atataaatg 
      tgggcgagt ttgtaaaca cattcaatt cggcctttg 
      aaacgtaca aaaaagcag acgaagcgg agaaatcca 
      aaactgcgt ctagtctta agactcggg aggatcttc 
      ctgaagtaa aagccgtaa cccaagaga ctgacatag 
      atttagtgt gtgatattg actcaaatg cacaattgc 
      caggaactt ccggtactc tagacattc ctactgtag 
      atatccaag atgacaaaa gtgatgatg gtagtgaga 
      ctgattgtg acgagccca cattgcgaa attttgata 
      ttcctttag ctgatggct ccgtagatg acgtagccg 
      gagaattaa aaaaggctt tagatgaac tgactagcc 
      aagacgaaa caaaacacg aaattaggc atcattttg 
      agactgcga gttttgatt ttgacgatg tagatgaac 
      agttgactg ttgcaatag aaaaagcca aagatagcc 
      caggtcgcg gacaagaag acaatcatg aacttttgg 
      atttgttga ataaagcag tcgacgact ctgacgtgg 
      atttgataa ctaagggcg atcaagcct tacagattt 
      tagaagagg atatgtcat tatcaagtt gtcaggatt 
      cattctcgg atgattctt tgtctgatg atgataatt 
      ccgataatg aagtaaacc gtaaaaacg aagataatg 
      ttgaagact tttgctctt ccgtagctg cagaagact 
      gccaaaatt tttctctaa aattacgac gcattgata 
      gcagtgatg gagaaatag atagattat gtaacacgg 
      acacactca aacgaaaat gtaaaaaaa taacagcac 
      gacatagga aaaagaaat acaggcgat taatacctt 
      tggacagtt cttcgtcgt catcatcgt ctgaggatg 
      aaaattcct cagatggtt ctgtaacgc ctgatctca 
      ttatagacg aacaacgcc cttcttcac ctgtttttg 
      gtgatgatg cggaaaatg aggcacttg cggctttgg 
      ccgataatt tagtggaca gattggact caggtgaat 
      caagttcag attcggaca ctactgagg aagaggagc 
      cagctaaga agaaaagga aaatgacgc ctgaaaata 
      tggatgaaa gtaaaaatg taaagacta caaccgaag 
      acaaggata cgagtagtt cgagtttgg acgaagaaa 
      cgattccaa aacgcaaaa gctggaaaa acgatcctt 
      tgcttcgca gttcgagct cagacagcg attcggatg 
      cagattcgt tggtattcc atgtgagag ataaaagaa 
      caaaagata acactgatc tgaacgaaa acgttgtca 
      aagttaata ataacagcg acaagagaa atgtcgtgg 
      aaattgaaa atcttgatt cagatcagg aaagtatta 
      gtgaaaata aaaatgaaa ttaaggtag caaataaca 
      atttcgaaa ctatgatta taactgatt cgtctgatt 
      caagttctg acagtgacg atgttctta tcgttgagg 
      atgaaacgg gtggaacga aagggcgca gaaacatca 
      gggctattt taagtgacg atgaactta acgaattaa 
      ctcagcaag ccaaaaatg aagaaaatg agagaatta 
      tgcgactcg ccgagcatt ccaaaaata tcgagtcaa 
      tacaatccc aacacgaag actttatat tagattttg 
      atgtaaaga ctggcgaac ctctagtca ccgtggacc 
      acaaactcg caaaaatgt tgaaacctc atcaaaaaa 
      gcggtattc aattcatgt gggaagcat gttacgaat 
      cagttgaaa ttttaaaaa ccaagccag gatcgggct 
      gcattctag cccattgca tgggcttag gaaaaaccc 
      tccaagtca tcactttaa tccacaccc tcttcacgc 
      atcctgtca caaaaacaa aacatgttc tggtggtgt 
      gtccgctgt ctactgtga tcaattgga agaacgagt 
      ttaagaaag ccttcaaac aattggata atccgcccg 
      atatttatc cttattgga ttattaaag gtgatatta 
      gtgagaaaa taaatatta tacatacgt ggcgccaaa 
      ctggtggtg ttttaatcc tgggttacg acgcttatc 
      aaattataa ccaacgaga aaacagctt taagaataa 
      cagctgtag aaaagcaac gggctttgg aagctctcg 
      ttgatccag gacctgatt tgattgttt gcgacgaag 
      gccaccaac ttaaaaatg gtaaaactc taaagacgc 
      aagctttaa tgaaggtga aaacgaaac gaaggattg 
      ttctcacag ggacgccac tacaaaata atctgaaag 
      aatattatt ttatggtcc agtttgtta agccccatc 
      ttttgggaa cgtacttag agtacacga acaggtttg 
      ctagtccaa ttatgaatg gacagtttc atgactcga 
      cacctggag atatcaaat tgatgaaga agaggacac 
      atgttctca ccaaaatgc tcaaaaaca caattcata 
      gagtggaag gtagtgtct tgtcaacgt atctgccag 
      aaattacag actatacga tattcataa aattaactc 
      cattgcaaa ttgatcttt acacccgtt acatagatt 
      tggtgactg gacaggcct ccaatctga gttcaacat 
      tcttccatg acgtgagaa tgacaaatt tctgcaatt 
      tacatccct acgctcttc acttacatt acagcaaac 
      caacgttga aaagaaagg cgaaaactg ctggcctga 
      ttgaaaatc tcgaaggtg atgacaata atgtggagc 
      atatcagag cagactggt acaaaaatc tgctaccag 
      cggatgttt ccacaaata taaattaca gtacaaaga 
      tcaaactca ttttggaca taattagcg aatgcatgc 
      gaaataatg agaaagttt tgatatttg gacaatatt 
      tggtcgaac ttgacatcg ttgagcatt tcctgaaac 
      agttcagga actggagac ccaatgtag actactaca 
      gaatggatg gcgacacgt ctgtggaaa atcgcgaca 
      tactgtgca aaaaattca attcgaatc ccacctcta 
      aagtatttc tcctcacac ataaagtgg gtggactgg 
      gcttgaatt tgacaggtg caaatcgag tgattctga 
      ttggttcga atcacaatc cctcccacg acagtcaga 
      gtttataca gagtgtatc ggttcggcc aagagagga 
      aatgttacg tttatcgac ttgtatcac tcgggacaa 
      tggaggaga aaatttacc acagatgcg tcttgaaat 
      tgtctatat ctggcaccg ttgtcgata agttgcatt 
      tcgatcgcc gttacaaaa cgaccgatt tgaaagaaa 
      tgtacaaat atgatttcg ataagtaca atagcgaaa 
      gaccaatac cagcggtgc caagtgata tgttgatgg 
      cactgctgt tgaaaaatt gtcccgata tcttcggtt 
      accacttac ataatgcct tacttgaaa accggccag 
      aagaggaac tgaccgatg aggacaaaa agttggctt 
      gggacgaat tcaacaatt tagcagagg cagcaagga 
      caaatgcac ccaatccgc ccaacattc caaacctag 
      tactcaatc ccatgagta ccaacacaa ggggttttg 
      taccaaaga ctcaagggc cctctaccc caaataacc 
      cgtttgtga atttgcttc ttggtcgta ttggacttg 
      aaaacggcg aattaaaaa agatcatgt cagaccgat 
      ttaaaatga caatggcta ataaaacac ctactactg 
      gcgaattta attacgcat taaataagt ttatttaac 
      tcattcgag ttgcgttta attaatttc aagtatttt 
      ttttcttaa agacaccct tccatttgc gctcaggtg 
      tatactaat aggctatta ccaagcttt acatagagt 
      aaataaggc aagaacaga ataagactt tgtttttta 
      atcattgtt tctggctta tttcttgaa caacacgtt 
      ttgtaatta gaactgata atcgatcat tcaataaaa 
      aatattgaa tacg
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