Status
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Gene information
General information
TC033630 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4653389..4655732
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033630
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09221

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033630 RA
      MDVEQVKKK LHTALKFGG FNIRREFCG KIVAKFLRE 
      GIDLENKHH FDNCVKNLC SSLENQCLL GHSIEKEHI 
      DRALEVCLK SGYDRNETI FSVINAFDF PKVSYNLDR 
      KQYYLTENK PRLLSSADS KAQLFADRY ATILHRTKR 
      NFIQKATEN TNIKLELQT VDYLLTLSN VSLNHTLVL 
      GSLLQVSEG KYYLEDPTG IVQLDLSHA VYYGGLFAE 
      NCFVLVNGH YEDKIIKVS TLILPPGEE YANSRPFFN 
      TINYFGGPS PTPLKDSVK LRDHLQRNR GASFMFFSN 
      VWLDHPSIF EKLELLFEG FNPSPPAAF VFMGNFMSE 
      SCSSNKMDT LRKLFKQLA ELINKYENL ATVTNFIFV 
      PGLEDPCTS YVVPRLPIP SCITEDMTK MVKKAVFTS 
      NPCRLQYCN KEITIFRAD VLPKLLQAS LHKPKKSEL 
      SENLTRTII SQGHLSPLP LNALTVHWD FDYTLSLNP 
      LPDLVVIGD KAEPYEGKY KGCQLINPG SFCESGFQF 
      KAYNPATDK IEECLL
    CDS sequence
    • >TC033630 RA
      atggacgtt gaacaagtc aaaaagaag ttgcacacg 
      gcgttaaaa ttcggcgga tttaatatt cggagggag 
      ttttgtggc aaaattgta gccaaattt ctcagagaa 
      ggtatcgat ttagaaaac aaacaccat tttgataat 
      tgtgttaaa aatctgtgc tccagttta gaaaaccag 
      tgccttctg gggcacagt atcgaaaag gagcacata 
      gacagggct ttggaggtt tgcctgaag tctggttac 
      gacaggaat gaaacaatt ttcagtgtt ataaacgct 
      ttcgatttt ccaaaagtt tcatacaat cttgataga 
      aaacagtat tatttaacc gaaaataag ccccgactt 
      ttgtcttct gcggattcc aaggcacag ctttttgcc 
      gacagatat gccacgatt ttacacagg accaaaaga 
      aattttata cagaaagca acagaaaac acgaatatt 
      aaattagaa ttgcaaact gttgattat ttgttaaca 
      ctttcgaat gtctcactg aaccatact ctggtatta 
      ggctcgtta ttgcaagtg tctgaaggc aaatattac 
      ttagaggac cccacggga atcgtccaa ctggactta 
      agccacgct gtttactat gggggctta tttgccgaa 
      aactgtttt gtgttagtt aacggacat tacgaagac 
      aagataatc aaagtctcc acgttaatt ctaccccca 
      ggggaggaa tacgccaat tcgcgcccc tttttcaac 
      acgataaat tatttcggg ggcccatct ccaacaccc 
      ctcaaagac tccgtaaaa ttacgcgat cacttacaa 
      cgaaatcgc ggcgcttcg ttcatgttt ttctcaaat 
      gtttggctt gaccaccct agtattttt gaaaaactt 
      gaattgctg tttgagggt ttcaacccc agcccgcct 
      gcggcgttc gttttcatg gggaatttc atgtcagag 
      tcttgcagc agcaataag atggacact ttgaggaaa 
      cttttcaaa caactagct gaattaatt aacaaatac 
      gagaatttg gccactgtt acgaatttt attttcgtg 
      ccgggtttg gaggatcca tgcacttca tacgtcgtg 
      ccaagactg ccgattccg tcatgcata actgaagat 
      atgacgaaa atggtgaaa aaggcagtt tttacgtct 
      aatccgtgc cgtttgcaa tattgcaat aaggaaatt 
      acgattttt agggccgat gttctccca aaattgcta 
      caagcgtca ttgcataaa ccgaaaaag agcgaattg 
      tccgaaaat ctgaccagg actataata agtcaaggg 
      catttgtcg cccttgccc ttaaacgct ttgacagtt 
      cattgggat tttgattac actctaagt ttaaatcct 
      ctccctgat ttagtagtg ataggagac aaggccgaa 
      ccttacgaa gggaaatac aaagggtgc caacttata 
      aaccctggt tcgttttgt gagagcggg ttccagttc 
      aaggcgtac aatcctgca acagacaaa atcgaggaa 
      tgtctactg tga
    mRNA sequence
    • >TC033630 RA
      gaatttggg cgggaaata attttaggt tatttattc 
      taaaaaatt cacaattta caaatactc aaagcagaa 
      atcaaagaa atggacgtt gaacaagtc aaaaagaag 
      ttgcacacg gcgttaaaa ttcggcgga tttaatatt 
      cggagggag ttttgtggc aaaattgta gccaaattt 
      ctcagagaa ggtatcgat ttagaaaac aaacaccat 
      tttgataat tgtgttaaa aatctgtgc tccagttta 
      gaaaaccag tgccttctg gggcacagt atcgaaaag 
      gagcacata gacagggct ttggaggtt tgcctgaag 
      tctggttac gacaggaat gaaacaatt ttcagtgtt 
      ataaacgct ttcgatttt ccaaaagtt tcatacaat 
      cttgataga aaacagtat tatttaacc gaaaataag 
      ccccgactt ttgtcttct gcggattcc aaggcacag 
      ctttttgcc gacagatat gccacgatt ttacacagg 
      accaaaaga aattttata cagaaagca acagaaaac 
      acgaatatt aaattagaa ttgcaaact gttgattat 
      ttgttaaca ctttcgaat gtctcactg aaccatact 
      ctggtatta ggctcgtta ttgcaagtg tctgaaggc 
      aaatattac ttagaggac cccacggga atcgtccaa 
      ctggactta agccacgct gtttactat gggggctta 
      tttgccgaa aactgtttt gtgttagtt aacggacat 
      tacgaagac aagataatc aaagtctcc acgttaatt 
      ctaccccca ggggaggaa tacgccaat tcgcgcccc 
      tttttcaac acgataaat tatttcggg ggcccatct 
      ccaacaccc ctcaaagac tccgtaaaa ttacgcgat 
      cacttacaa cgaaatcgc ggcgcttcg ttcatgttt 
      ttctcaaat gtttggctt gaccaccct agtattttt 
      gaaaaactt gaattgctg tttgagggt ttcaacccc 
      agcccgcct gcggcgttc gttttcatg gggaatttc 
      atgtcagag tcttgcagc agcaataag atggacact 
      ttgaggaaa cttttcaaa caactagct gaattaatt 
      aacaaatac gagaatttg gccactgtt acgaatttt 
      attttcgtg ccgggtttg gaggatcca tgcacttca 
      tacgtcgtg ccaagactg ccgattccg tcatgcata 
      actgaagat atgacgaaa atggtgaaa aaggcagtt 
      tttacgtct aatccgtgc cgtttgcaa tattgcaat 
      aaggaaatt acgattttt agggccgat gttctccca 
      aaattgcta caagcgtca ttgcataaa ccgaaaaag 
      agcgaattg tccgaaaat ctgaccagg actataata 
      agtcaaggg catttgtcg cccttgccc ttaaacgct 
      ttgacagtt cattgggat tttgattac actctaagt 
      ttaaatcct ctccctgat ttagtagtg ataggagac 
      aaggccgaa ccttacgaa gggaaatac aaagggtgc 
      caacttata aaccctggt tcgttttgt gagagcggg 
      ttccagttc aaggcgtac aatcctgca acagacaaa 
      atcgaggaa tgtctactg tgaaagcct taaaatagc 
      atataaatt tattagtta ggtctcaaa cataatcta 
      gattgtaca cttgaaaat tacttgtac actatttac 
      attcacatt aaaatagtc gcctcggcg ataaatgaa 
      ttaaataac ctattgtaa taccgccta aatcaatac 
      gtattaaag aatttcaat ttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY