Status
Please wait ...
Gene information
General information
TC015909 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4111670..4112862
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015909
    iBeetle screen iB_09218

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015909 RA
      MKIVAFGNP LLDTIVLLK SDALLKKYC LEKDGQKEV 
      SQSEMKALV ADLEEYEKT FVAGGCAQN TLKVVQWLL 
      DKKCAATMF GSIGGDSEG EILKKILRE HGVDTNYVT 
      QPGYITGST VSLVTGESR SLVAYLGAA EVMSPQDFS 
      PNSHIIEDA NLVYMEGFF ITKRIQVAS AIVNCCNRL 
      NKQFVFNLS GQYLCTDYT QTVVDFVQK SSIIFGNKR 
      EFQTICPPM NESSVENLI KNLGKQGKI VVVTDGAKC 
      VNCVGGGET LEIKVPELR AEEIVDTTG AGDAFVAGF 
      LSGYLLKKP LKTCCELGN YAAQEIIKR RGCTIPDYP 
      PVLK
    CDS sequence
    • >TC015909 RA
      atgaaaatt gttgcgttt gggaacccc ctactggac 
      acgatcgtg ctgttaaaa agcgacgct cttttgaaa 
      aaatattgt ttggaaaaa gacggccag aaagaagtg 
      agtcagagc gaaatgaag gcattggtg gccgatctt 
      gaagagtac gaaaaaact ttcgtggct ggcggttgt 
      gcacaaaac acgctcaag gtggttcag tggcttttg 
      gacaaaaaa tgcgctgca acaatgttt ggctcgatt 
      gggggcgac agtgagggg gaaattctg aagaaaatt 
      ttacgggag cacggggta gacacgaat tatgtcaca 
      cagcctggg tacattacg ggatcaact gtgtctctg 
      gtaactggg gaaagtcga tcgttggtt gcttatctg 
      ggcgctgct gaagtgatg tctcctcag gatttttcc 
      ccgaattcg cacataatt gaagacgcg aatttggtg 
      tacatggag gggtttttc attaccaag aggattcaa 
      gttgcttcc gctatcgta aactgctgt aatcggctc 
      aacaaacag tttgtgttt aacttgtcg gggcaatat 
      ttatgcaca gactacaca caaaccgtt gttgatttt 
      gtacaaaaa tctagcatt atctttggt aataagagg 
      gaattccaa actatttgt cccccaatg aacgaaagt 
      agtgttgaa aatttgatt aaaaacttg ggaaaacag 
      ggtaaaatt gtggttgtt actgacggt gccaaatgt 
      gtgaattgt gtgggaggg ggcgaaact ttggaaatc 
      aaagtgcca gaattgagg gccgaggaa attgttgat 
      acgaccggg gctggggac gcatttgtg gcaggtttc 
      ctaagtggg tatctcctc aaaaagccg cttaaaacg 
      tgctgtgaa ttgggaaat tacgccgcg caggaaatt 
      atcaaacga aggggttgc acaataccc gactacccc 
      cctgtactt aaatag
    mRNA sequence
    • >TC015909 RA
      agataaaaa taattgtcg caatgataa tgcccccaa 
      tgattactt aatctccca atgcacatg ttcattttt 
      tgcaaataa ttacatcag ttccccatt ttttcgtaa 
      ctagagtga gtgtgctgt ttttcactg aaaatgaaa 
      attgttgcg tttgggaac cccctactg gacacgatc 
      gtgctgtta aaaagcgac gctcttttg aaaaaatat 
      tgtttggaa aaagacggc cagaaagaa gtgagtcag 
      agcgaaatg aaggcattg gtggccgat cttgaagag 
      tacgaaaaa actttcgtg gctggcggt tgtgcacaa 
      aacacgctc aaggtggtt cagtggctt ttggacaaa 
      aaatgcgct gcaacaatg tttggctcg attgggggc 
      gacagtgag ggggaaatt ctgaagaaa attttacgg 
      gagcacggg gtagacacg aattatgtc acacagcct 
      gggtacatt acgggatca actgtgtct ctggtaact 
      ggggaaagt cgatcgttg gttgcttat ctgggcgct 
      gctgaagtg atgtctcct caggatttt tccccgaat 
      tcgcacata attgaagac gcgaatttg gtgtacatg 
      gaggggttt ttcattacc aagaggatt caagttgct 
      tccgctatc gtaaactgc tgtaatcgg ctcaacaaa 
      cagtttgtg tttaacttg tcggggcaa tatttatgc 
      acagactac acacaaacc gttgttgat tttgtacaa 
      aaatctagc attatcttt ggtaataag agggaattc 
      caaactatt tgtccccca atgaacgaa agtagtgtt 
      gaaaatttg attaaaaac ttgggaaaa cagggtaaa 
      attgtggtt gttactgac ggtgccaaa tgtgtgaat 
      tgtgtggga gggggcgaa actttggaa atcaaagtg 
      ccagaattg agggccgag gaaattgtt gatacgacc 
      ggggctggg gacgcattt gtggcaggt ttcctaagt 
      gggtatctc ctcaaaaag ccgcttaaa acgtgctgt 
      gaattggga aattacgcc gcgcaggaa attatcaaa 
      cgaaggggt tgcacaata cccgactac ccccctgta 
      cttaaatag ttaataaaa aaattacaa aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY