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Gene information
General information
TC034776 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4167151..4169494
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034776
    iBeetle screen iB_09217

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: >5 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034776 RA
      MFVTHILFI FCAISRVTS EIKISDEDY KLLPPIFHI 
      DPYDKCLQE NKTFCRISI KLSPSKNKT KIWNLIEET 
      KISKRFYNH DELYYAICV ENGQKNETL SELYTNKFK 
      NASLRATVV EINCQEHKY EIKTFDILV LTCIILYLL 
      LILVATLYH LKTPNGHKF LILFSPWHN FQNLKRNFK 
      QRNCEKLKC IQGIRFYTT VLIIFCHTF CSYSGGYIA 
      NTKYLEDFP QNFIRHGLR NLFVFLVQT FFLVSGWLL 
      SYNTFKIFD KTKRGDFFK YWCLTFLNR YLRLIPPVL 
      LMVSLGTSV WIYGSFHGP IKYVYSDRE YERCQRSWW 
      VIFLFLSNH YQQHDMCYF TNWYLSADT QLYAISLVL 
      LIIILKFER LRVRIMTIF LLIGIFIPS LVAFFYELD 
      IIYRITPEN SKNNLFQSF EFSATYTST YSTWATYIL 
      GLILGYVYF RLENRPVFG KKHFVLLWY ILVIGLPLS 
      VIIISSFEF NRVASALLA GILKPLYAL GIGIGIFGM 
      SQQIGGFLK SLCEWKPAL FLGNVTYST YVVHFGIVF 
      SRTALATKP LPVSDYVLF VSFVEDTVW SFLCGFLMH 
      LLVEMPASQ LQKMLVPKF SHGSEKKIK K
    CDS sequence
    • >TC034776 RA
      atgtttgtg acacatatc ctattcatt ttctgtgcc 
      atttcacgt gtcacaagt gagataaaa atatcagat 
      gaagattat aagttactg ccgccaata tttcacatc 
      gatccttac gacaaatgt ctacaggaa aataaaact 
      ttttgcagg ataagtatc aaattatca ccaagcaaa 
      aacaagaca aaaatctgg aatctaatt gaggagacg 
      aaaatctcg aagcgtttc tacaaccat gacgaactt 
      tattacgca atttgcgtc gaaaatggc caaaagaac 
      gaaacttta tcggaactt tacaccaac aagttcaaa 
      aatgccagt ttacgagca acagttgtg gaaataaac 
      tgccaagag cacaaatac gaaataaaa acgttcgat 
      attctagtt ttaacttgt attattctg tacttatta 
      ttgatactt gtggcgaca ttgtatcac ttgaaaaca 
      cccaatggg cacaaattc ctgattttg ttttccccg 
      tggcacaat ttccaaaac ttgaaacgc aattttaag 
      caaagaaat tgcgaaaaa ttgaaatgt atccaagga 
      attcgcttc tataccacc gttcttatt attttctgc 
      cacacgttt tgcagttac tctggtggt tacatcgcc 
      aataccaaa tatctggaa gactttccg caaaatttc 
      atcagacat ggtttgaga aacttgttt gtttttttg 
      gtacaaacc ttctttctg gtctcaggt tggctctta 
      tcgtacaac acgttcaaa attttcgat aaaacaaaa 
      cggggcgat tttttcaag tattggtgt ctcacgttt 
      ttgaaccga tatttgagg ttgataccg ccagtttta 
      ttgatggta tcgctgggc acaagtgtc tggatctat 
      gggtctttc catgggccc attaagtac gtttactcc 
      gatcgggag tatgaacgt tgtcagaga agctggtgg 
      gtgattttt ttatttctg agcaatcat taccaacaa 
      catgacatg tgttacttc accaattgg tacctgtct 
      gccgataca caactttac gcaatctcg ttagttctc 
      ctaataatt attttaaaa ttcgaacgt ttgagggtt 
      cgaataatg acaattttt ctactcatt ggcattttt 
      attccatca ctggtcgca tttttctac gaacttgac 
      attatttac agaattacg cccgagaat tcaaaaaac 
      aatctcttc caatcattc gaattcagc gccacttac 
      acgtccact tactcaaca tgggccacc tacatttta 
      ggattaata ctcggttat gtctatttt cggcttgaa 
      aataggcca gtttttggc aaaaaacat tttgtcctc 
      ttgtggtac attttagtc ataggtctc ccgttatcg 
      gtaataata atttcctcg tttgaattt aatcgagtc 
      gcctcggca cttttggcg gggattttg aaaccactg 
      tatgcctta gggattggg atcgggatt tttggcatg 
      tcgcaacaa attgggggt tttttgaaa tcgttgtgc 
      gaatggaag ccagcttta tttttgggg aacgtcact 
      tacagcacc tatgttgtg cattttggg attgttttt 
      agcaggaca gcgttggcc acgaaacca ctgccagtt 
      tctgactac gttttgttt gtttcgttt gttgaggac 
      acggtctgg tcgtttctt tgcgggttt ttgatgcat 
      ctgctggtg gaaatgcca gcgtctcag cttcagaaa 
      atgttagtt cctaaattt agccacgga tctgagaag 
      aaaattaag aagtga
    mRNA sequence
    • >TC034776 RA
      ctcacattt tattttaaa aaatgtttg tgacacata 
      tcctattca ttttctgtg ccatttcac gtgtcacaa 
      gtgagataa aaatatcag atgaagatt ataagttac 
      tgccgccaa tatttcaca tcgatcctt acgacaaat 
      gtctacagg aaaataaaa ctttttgca ggataagta 
      tcaaattat caccaagca aaaacaaga caaaaatct 
      ggaatctaa ttgaggaga cgaaaatct cgaagcgtt 
      tctacaacc atgacgaac tttattacg caatttgcg 
      tcgaaaatg gccaaaaga acgaaactt tatcggaac 
      tttacacca acaagttca aaaatgcca gtttacgag 
      caacagttg tggaaataa actgccaag agcacaaat 
      acgaaataa aaacgttcg atattctag ttttaactt 
      gtattattc tgtacttat tattgatac ttgtggcga 
      cattgtatc acttgaaaa cacccaatg ggcacaaat 
      tcctgattt tgttttccc cgtggcaca atttccaaa 
      acttgaaac gcaatttta agcaaagaa attgcgaaa 
      aattgaaat gtatccaag gaattcgct tctatacca 
      ccgttctta ttattttct gccacacgt tttgcagtt 
      actctggtg gttacatcg ccaatacca aatatctgg 
      aagactttc cgcaaaatt tcatcagac atggtttga 
      gaaacttgt ttgtttttt tggtacaaa ccttctttc 
      tggtctcag gttggctct tatcgtaca acacgttca 
      aaattttcg ataaaacaa aacggggcg attttttca 
      agtattggt gtctcacgt ttttgaacc gatatttga 
      ggttgatac cgccagttt tattgatgg tatcgctgg 
      gcacaagtg tctggatct atgggtctt tccatgggc 
      ccattaagt acgtttact ccgatcggg agtatgaac 
      gttgtcaga gaagctggt gggtgattt ttttatttc 
      tgagcaatc attaccaac aacatgaca tgtgttact 
      tcaccaatt ggtacctgt ctgccgata cacaacttt 
      acgcaatct cgttagttc tcctaataa ttattttaa 
      aattcgaac gtttgaggg ttcgaataa tgacaattt 
      ttctactca ttggcattt ttattccat cactggtcg 
      catttttct acgaacttg acattattt acagaatta 
      cgcccgaga attcaaaaa acaatctct tccaatcat 
      tcgaattca gcgccactt acacgtcca cttactcaa 
      catgggcca cctacattt taggattaa tactcggtt 
      atgtctatt ttcggcttg aaaataggc cagtttttg 
      gcaaaaaac attttgtcc tcttgtggt acattttag 
      tcataggtc tcccgttat cggtaataa taatttcct 
      cgtttgaat ttaatcgag tcgcctcgg cacttttgg 
      cggggattt tgaaaccac tgtatgcct tagggattg 
      ggatcggga tttttggca tgtcgcaac aaattgggg 
      gttttttga aatcgttgt gcgaatgga agccagctt 
      tatttttgg ggaacgtca cttacagca cctatgttg 
      tgcattttg ggattgttt ttagcagga cagcgttgg 
      ccacgaaac cactgccag tttctgact acgttttgt 
      ttgtttcgt ttgttgagg acacggtct ggtcgtttc 
      tttgcgggt ttttgatgc atctgctgg tggaaatgc 
      cagcgtctc agcttcaga aaatgttag ttcctaaat 
      ttagccacg gatctgaga agaaaatta agaagtgac 
      ttttgaaga attgtttct ataatagtt actaataaa 
      agaattggc taaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY