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Gene information
General information
TC015960 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3926073..3928879
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015960
    iBeetle screen iB_09214

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015960 RA
      MLIIAAAHL VYCPFTKVE ESFNLQAMH DILYHKWNL 
      SQYDHLEFP GVVPRTFIG PLFISILVA PIVSLLQLF 
      EINKFWAQY IVRFALGGC VIAAFHVLS RTLAKIFGP 
      KWQLWFMAV TVTQSHFMF YLSRPLPNI FALPLVLLA 
      LNSWLKGDS KQFILLSGA AIIIFRAEL ALFLGLLLL 
      YDLINKRIT LKRFFQVAL PAGVAFLLL SVVIDSIFW 
      NRPLWPEGE VLWYNTVLN KSSNWGTSP FFWYFYSAI 
      PRGMATSVF LIPLGLYFD ERVRKLCVP TLLFVLLFS 
      FLPHKELRF IIYVFPFLN ATAATACHR IWENRNKSP 
      IYHLLSLGV CGHLAVNVF FTLFLLSIS GTNYPGGTA 
      ISHLHRLAK DEANVSVHI ANLAAQTGV SRFTQINPN 
      WTYSKVEGV MAGAPEMYE YTHLIAEAR SKFSPNLKP 
      YASTHDIID TIEAFHQLS FNYLTIPPI KIKTRPVLF 
      ILKRRENYR EFLQMRNYD FEDGMLEET IIEDESQEE 
      EKIQEAESE QNVTIDEVI VPKNEETNV TNTTSFEDV 
      KNETKSEVV KKQSKPINV TKKLKPVED KPLNSTEER 
      QTVRRNIKK LIQKYRRKK ESDGELESK PKDTIKKII 
      EEEKMKQAE EELSKIQQQ IFDIIETNP NIINKKLIK 
      QRLRETITS EFMNVKKLK RVKAKPRQV DDSAVDFYE 
      PPQDEVKEP TPETEDEEK LNQMSERFL EASKKIADI 
      MTIIDEIVD TMEISEDSE EV
    CDS sequence
    • >TC015960 RA
      atgctaatt atcgccgcc gcccatttg gtatactgc 
      ccatttacc aaagtggag gaaagcttc aatttacag 
      gccatgcac gatattttg taccacaag tggaacctg 
      tctcagtac gaccatttg gagttcccc ggagtggtg 
      ccgcggacg ttcatcggc cccttgttc atttcgatt 
      ttggtggcc cccatagtc tcgcttttg cagttgttc 
      gaaattaac aaattttgg gcgcaatat atcgtgcgt 
      tttgctttg gggggctgc gttatcgcc gctttccac 
      gtcctgtcc cgcactttg gccaagatt ttcgggccg 
      aagtggcag ctgtggttc atggcagtg acggtgacc 
      cagagccac tttatgttt tacttgagc cggcccttg 
      cccaacatt ttcgcactg ccgctggtg ctccttgcg 
      ttgaatagt tggctcaaa ggcgacagc aaacaattt 
      attttgctt tcgggggcc gctattatc atattcagg 
      gccgaactg gccctcttc ctcggccta ctcctactg 
      tacgatttg atcaataaa cgaatcact ctcaaacgg 
      tttttccaa gtggcgctt ccggccggt gtcgcattt 
      ttgctactt tcggtcgtt atcgactcg attttttgg 
      aacaggccg ctgtggcct gaaggagaa gtgttatgg 
      tacaacacc gtcctgaac aagagctca aattggggc 
      acatcgccg tttttctgg tatttttac tcggcgatt 
      ccgcgagga atggcaact tcagtattt ctaatccca 
      ctgggattg tatttcgac gaacgtgtc cgaaaatta 
      tgcgttccc acgctttta ttcgttctc ttattctcg 
      tttttgccc cacaaagaa ctcagattc attatctac 
      gttttcccg ttcctcaac gccacagca gcgacggct 
      tgccaccga atatgggaa aatcggaac aagagcccc 
      atttaccac ttgttatcg ctgggcgtt tgcggccat 
      ttggccgtg aacgtgttt ttcactttg tttctattg 
      agcatttct gggacgaat tatccggga gggaccgca 
      atttcgcac ttgcatcga cttgccaaa gacgaagcc 
      aatgtttcg gttcatatc gcaaatcta gcggcacaa 
      accggtgtc tcacgtttt acgcaaatc aatccgaat 
      tggacttat agcaaagtt gagggtgtt atggctggg 
      gctcccgaa atgtacgag tatacgcat ttaattgcc 
      gaagccaga agtaaattt tcgccaaat ttgaaacct 
      tatgcttcc acacacgac attattgat acaatcgag 
      gcgtttcat caattgtcc tttaattat cttacaatt 
      cccccgatt aaaattaag acaaggcct gtcttgttt 
      atcttgaag cgaagggag aattatcgc gagtttttg 
      caaatgagg aattacgat tttgaggac ggaatgctt 
      gaagaaaca ataattgaa gacgagagt caagaagag 
      gaaaaaatt caagaagct gaatcggaa caaaacgtg 
      acaattgac gaagtcatt gtgcccaaa aacgaagaa 
      acaaatgtg acaaacaca accagcttc gaagatgtg 
      aaaaacgag accaaatca gaagtggta aagaaacaa 
      tcaaagcca ataaacgtg acgaaaaaa ttgaagccg 
      gttgaggac aagcccctc aacagcacc gaagaaaga 
      cagactgtg cggcgaaac atcaaaaaa ttgattcag 
      aagtacagg aggaaaaag gaaagtgac ggcgaactc 
      gagtcgaag cccaaagac acgatcaag aaaatcatc 
      gaagaagag aaaatgaag caagcggag gaagaattg 
      agcaaaatt cagcaacag atcttcgat attatcgaa 
      acgaaccca aacatcatc aacaagaag ttgattaag 
      cagcgattg cgcgaaaca atcactagc gaatttatg 
      aacgtgaaa aaactgaag agagtcaag gcgaagccg 
      aggcaagtg gacgacagt gctgtggat ttctacgaa 
      ccgccacaa gacgaagtc aaagaacca acccccgaa 
      actgaagat gaagaaaag ttgaaccaa atgtcggaa 
      cggtttctg gaggcgagc aagaaaatt gccgatatt 
      atgacaatc attgatgaa attgttgac acgatggag 
      atttcggag gatagtgag gaggtttaa
    mRNA sequence
    • >TC015960 RA
      accgaagag ataaaataa atttagctt atttacgtc 
      agctcattt gaaattgta ggttaggtg aatctattt 
      tcgtccaaa attcaagaa tgtagataa aaattagcc 
      cccgaaatg gtgcaaata atgctaatt atcgccgcc 
      gcccatttg gtatactgc ccatttacc aaagtggag 
      gaaagcttc aatttacag gccatgcac gatattttg 
      taccacaag tggaacctg tctcagtac gaccatttg 
      gagttcccc ggagtggtg ccgcggacg ttcatcggc 
      cccttgttc atttcgatt ttggtggcc cccatagtc 
      tcgcttttg cagttgttc gaaattaac aaattttgg 
      gcgcaatat atcgtgcgt tttgctttg gggggctgc 
      gttatcgcc gctttccac gtcctgtcc cgcactttg 
      gccaagatt ttcgggccg aagtggcag ctgtggttc 
      atggcagtg acggtgacc cagagccac tttatgttt 
      tacttgagc cggcccttg cccaacatt ttcgcactg 
      ccgctggtg ctccttgcg ttgaatagt tggctcaaa 
      ggcgacagc aaacaattt attttgctt tcgggggcc 
      gctattatc atattcagg gccgaactg gccctcttc 
      ctcggccta ctcctactg tacgatttg atcaataaa 
      cgaatcact ctcaaacgg tttttccaa gtggcgctt 
      ccggccggt gtcgcattt ttgctactt tcggtcgtt 
      atcgactcg attttttgg aacaggccg ctgtggcct 
      gaaggagaa gtgttatgg tacaacacc gtcctgaac 
      aagagctca aattggggc acatcgccg tttttctgg 
      tatttttac tcggcgatt ccgcgagga atggcaact 
      tcagtattt ctaatccca ctgggattg tatttcgac 
      gaacgtgtc cgaaaatta tgcgttccc acgctttta 
      ttcgttctc ttattctcg tttttgccc cacaaagaa 
      ctcagattc attatctac gttttcccg ttcctcaac 
      gccacagca gcgacggct tgccaccga atatgggaa 
      aatcggaac aagagcccc atttaccac ttgttatcg 
      ctgggcgtt tgcggccat ttggccgtg aacgtgttt 
      ttcactttg tttctattg agcatttct gggacgaat 
      tatccggga gggaccgca atttcgcac ttgcatcga 
      cttgccaaa gacgaagcc aatgtttcg gttcatatc 
      gcaaatcta gcggcacaa accggtgtc tcacgtttt 
      acgcaaatc aatccgaat tggacttat agcaaagtt 
      gagggtgtt atggctggg gctcccgaa atgtacgag 
      tatacgcat ttaattgcc gaagccaga agtaaattt 
      tcgccaaat ttgaaacct tatgcttcc acacacgac 
      attattgat acaatcgag gcgtttcat caattgtcc 
      tttaattat cttacaatt cccccgatt aaaattaag 
      acaaggcct gtcttgttt atcttgaag cgaagggag 
      aattatcgc gagtttttg caaatgagg aattacgat 
      tttgaggac ggaatgctt gaagaaaca ataattgaa 
      gacgagagt caagaagag gaaaaaatt caagaagct 
      gaatcggaa caaaacgtg acaattgac gaagtcatt 
      gtgcccaaa aacgaagaa acaaatgtg acaaacaca 
      accagcttc gaagatgtg aaaaacgag accaaatca 
      gaagtggta aagaaacaa tcaaagcca ataaacgtg 
      acgaaaaaa ttgaagccg gttgaggac aagcccctc 
      aacagcacc gaagaaaga cagactgtg cggcgaaac 
      atcaaaaaa ttgattcag aagtacagg aggaaaaag 
      gaaagtgac ggcgaactc gagtcgaag cccaaagac 
      acgatcaag aaaatcatc gaagaagag aaaatgaag 
      caagcggag gaagaattg agcaaaatt cagcaacag 
      atcttcgat attatcgaa acgaaccca aacatcatc 
      aacaagaag ttgattaag cagcgattg cgcgaaaca 
      atcactagc gaatttatg aacgtgaaa aaactgaag 
      agagtcaag gcgaagccg aggcaagtg gacgacagt 
      gctgtggat ttctacgaa ccgccacaa gacgaagtc 
      aaagaacca acccccgaa actgaagat gaagaaaag 
      ttgaaccaa atgtcggaa cggtttctg gaggcgagc 
      aagaaaatt gccgatatt atgacaatc attgatgaa 
      attgttgac acgatggag atttcggag gatagtgag 
      gaggtttaa aatgtaaag aaatgtttg tttaaata
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