Status
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Gene information
General information
TC033603 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3892982..3894848
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033603
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09212

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033603 RA
      MNPLFVPSS EMTFFQRVV NTLNVWADY CLTSPILTT 
      QQQSLVTKH FPDAPTLQH LVEKVALVF VNSHYSIED 
      PRPYVPNLI QVGGLHVDE PKQLPQELK GFLDAAKTG 
      AVFFSLGSN VKISSLGGE KLRAILTVL GALPMKVLF 
      KTDENLDNL PPNVKVGKW LPQNDILGH PNVKLFVSH 
      GGLLSTIEA VYHGVPIIG IPIFGDQRR NIEDCVRKG 
      FAIKVELSD LNEQLFADS IEEMLENPK YRENVQLRS 
      SLIRGEPIK PLDKAVHWV EHVLKYKGA EHLKNASTK 
      LNLLQYLLI DVVAFIVAV FLLVLLILY KCVKGVVFL 
      VCKVTRRGE KAKKE
    CDS sequence
    • >TC033603 RA
      atgaaccca cttttcgtc cctagttcc gaaatgacc 
      tttttccaa cgcgtcgtc aacacgtta aacgtctgg 
      gcggattat tgcctcacc tcacctatt ttaacaacc 
      cagcaacaa tcactcgta accaaacac ttcccggac 
      gcccccact ttacaacac ttggtcgaa aaagtggct 
      ttggtgttc gtcaactcg cattacagc atcgaagat 
      cccagacct tacgtccca aacttgata caagtcggt 
      ggcctgcac gtggacgaa cccaaacaa ctgccacaa 
      gaactgaag gggtttctg gacgctgcc aaaaccggc 
      gctgttttc ttcagtctg ggctccaac gtcaaaatt 
      tccagccta gggggggag aaactacgg gcgattttg 
      acagttttg ggtgctctt ccgatgaaa gttttattc 
      aaaactgac gaaaacttg gacaatttg cccccaaat 
      gttaaagtg ggtaagtgg ttgccccag aatgatatt 
      ttggggcac ccgaacgta aaactgttt gtctcacat 
      gggggcttg ttgagcaca attgaggcc gtttaccac 
      ggggtgcca attattgga attccgatt tttggcgac 
      cagaggcgg aatattgaa gattgtgtg cggaaaggt 
      tttgcaatt aaagtcgaa ctgagtgat ttaaacgag 
      cagttattt gccgattcg attgaagaa atgttggaa 
      aatccaaag tatcgggaa aacgtccaa cttcggtcg 
      tcgcttatc agaggtgag ccgataaaa ccgcttgat 
      aaggcggtt cattgggtg gagcacgtg ttgaagtac 
      aagggggcc gaacatttg aaaaatgcc tcaactaag 
      ttgaatcta ctccagtat ttgttaatc gatgtggtt 
      gcgtttatt gtcgctgtc tttctcttg gtgttgctt 
      attttgtac aaatgcgtg aagggagtt gtgtttttg 
      gtgtgtaaa gtgacaagg aggggggag aaggcgaag 
      aaagagtga
    mRNA sequence
    • >TC033603 RA
      tttcggaaa gcttgctcg ggctgccca ccaatggga 
      cgcccctgt gatcctctt caacacttt agggactgt 
      cccggccct cgaccgcat caccggaaa cccctcccc 
      tccttcctt cgtccccac aatgaaccc acttttcgt 
      ccctagttc cgaaatgac ctttttcca acgcgtcgt 
      caacacgtt aaacgtctg ggcggatta ttgcctcac 
      ctcacctat tttaacaac ccagcaaca atcactcgt 
      aaccaaaca cttcccgga cgcccccac tttacaaca 
      cttggtcga aaaagtggc tttggtgtt cgtcaactc 
      gcattacag catcgaaga tcccagacc ttacgtccc 
      aaacttgat acaagtcgg tggcctgca cgtggacga 
      acccaaaca actgccaca agaactgaa ggggtttct 
      ggacgctgc caaaaccgg cgctgtttt cttcagtct 
      gggctccaa cgtcaaaat ttccagcct aggggggga 
      gaaactacg ggcgatttt gacagtttt gggtgctct 
      tccgatgaa agttttatt caaaactga cgaaaactt 
      ggacaattt gcccccaaa tgttaaagt gggtaagtg 
      gttgcccca gaatgatat tttggggca cccgaacgt 
      aaaactgtt tgtctcaca tgggggctt gttgagcac 
      aattgaggc cgtttacca cggggtgcc aattattgg 
      aattccgat ttttggcga ccagaggcg gaatattga 
      agattgtgt gcggaaagg ttttgcaat taaagtcga 
      actgagtga tttaaacga gcagttatt tgccgattc 
      gattgaaga aatgttgga aaatccaaa gtatcggga 
      aaacgtcca acttcggtc gtcgcttat cagaggtga 
      gccgataaa accgcttga taaggcggt tcattgggt 
      ggagcacgt gttgaagta caagggggc cgaacattt 
      gaaaaatgc ctcaactaa gttgaatct actccagta 
      tttgttaat cgatgtggt tgcgtttat tgtcgctgt 
      ctttctctt ggtgttgct tattttgta caaatgcgt 
      gaagggagt tgtgttttt ggtgtgtaa agtgacaag 
      gagggggga gaaggcgaa gaaagagtg aaataatca 
      aaggtttaa catccaaat atacgccat tttattata 
      gctctaaca aaatcataa agtataata caaaatgct 
      atcgtaact acttacaat tctttttat acacaacaa 
      ataactaat cttataatg aaaatgttt aaacgcata 
      atctattaa atctctgta ttgctattt aattacaag 
      tgtcactgt aaaggattt ataggcatg ttatagtgt 
      aggtaagta atgccaatg atggttttc tagaaatat 
      gggtttcta tacaatcat aaaacaggc acggatcaa 
      ctaaatgtg gaaaaagtc ggtgtaaca gcactttag 
      gtgaggttc tacgtctaa atgctcaat gaactacgt 
      ttcgattga tatgatatg atattattt gtttcaaat 
      gcagtctct tgatccttt cgcgctggt tttgccctc 
      ttcaccgcc aatggacac gtttaatta aaatggtta 
      agttttagt gttacacaa aatacaaaa attaaacac 
      ttcagcgaa atcgttgat taaaattta ttgtaaaga 
      taagtacaa ttattcatt tctctaaaa atttactag 
      caattttct tcttatttt ttaaggaac tataactag 
      aacgccacc ctccataag gtttattaa agcggtaat 
      ttttattaa attatttcc taattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY