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Gene information
General information
TC034773 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3816478..3819975
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034773
    iBeetle screen iB_09210

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034773 RA
      MEGFDDPGV FYSDNFTVE DNQSDPQIN LQGVKKKFK 
      EFIRQYHTD NFSYKYRDT LKRNYNLRQ YYLEVNIED 
      VGAFDETLA DKLYKHPSE HLVIFEEAA KEVADELTA 
      PRPEGEEAV EDIQIMLSS DATPTNLRN MKSDVVSKL 
      VKIPGIVVS ASGIKAKAT KIAIQCRSC QNVIPNLPV 
      KPGMEGYVM PRKCNTEQA GQAKCPLDP YFIIPDKCH 
      CVDFQILKL QELPDGIPQ GEIPRHLTL YCDRYLCEK 
      VVPGNRIFV LGIYSIKKI AKVGRRDDR ERGTTGVRA 
      PYIRVVGVQ LDTEGVGGS PTSVVTAEE ENMFRRMAD 
      SPNIYERIA NSIAPSIFG ALDIKKAIA CLLFGGSRK 
      RLPDGLTRR GDINILLLG DPGTAKSQL LKFVERVAP 
      IGVYTSGKG SSAAGLTAS VMREPSSRN FVMEGGAMV 
      LADGGVVCI DEFDKMRED DRVAIHEAM EQQTISIAK 
      AGITTTLNS RCSVLAAAN SIFGRWDET KGEENIDFM 
      PTILSRFDM IFIVKDEHD QQRDMTLAR HIMGVHMNA 
      GEVTNPGTV NFRSNSSKN TSDIAGPVV APV
    CDS sequence
    • >TC034773 RA
      atggaaggt ttcgatgat ccaggagtg ttttacagt 
      gataatttc acggttgag gacaaccaa agcgacccc 
      caaatcaat ttacaaggt gttaagaaa aagttcaag 
      gagttcatc cgacaatac cacaccgac aatttcagc 
      tacaaatac agggacacc ctgaagcgc aattacaac 
      ctgcgccaa tattacctg gaagtcaac attgaagat 
      gttggcgct tttgacgag acgttggcc gacaaacta 
      tacaaacac ccgtcggaa cacttggtt atatttgag 
      gaagcggcc aaggaagtg gccgacgaa ctcacagcc 
      ccacgtccc gaaggagag gaagcagtt gaagacata 
      caaattatg ctgtcttca gacgccact ccgacaaac 
      ttaaggaac atgaaatcg gacgttgtc tcaaaacta 
      gtgaaaatc ccagggatt gtggtcagt gcgtcaggg 
      attaaagcc aaagctacg aaaatcgca atccagtgc 
      cgttcgtgc cagaacgta atccccaat ttacctgtg 
      aagcccggg atggaaggc tatgttatg ccacgcaaa 
      tgcaacacg gagcaagca ggccaggcc aagtgcccc 
      ttggacccc tacttcata atccctgac aaatgccac 
      tgcgtcgat ttccaaatc ctcaaattg caagaacta 
      ccagacgga atccctcaa ggggaaatc ccccgacac 
      ctcaccctc tactgcgac cgctacttg tgcgagaaa 
      gtggtcccc gggaaccga atcttcgtc ttggggatt 
      tactcgatc aagaaaatc gcaaaagtg ggccggcgt 
      gatgaccgt gagcgcggc acaacgggc gtccgagcc 
      ccgtatatt cgcgtcgtg ggggtgcaa ctcgacact 
      gaaggtgtc ggtggttcc ccgacttct gtcgtgacc 
      gccgaagaa gaaaacatg tttcggcga atggccgat 
      tcgcccaat atttacgaa cgaatcgcc aatagcatc 
      gcaccgtcg attttcgga gctcttgat attaagaaa 
      gccatagct tgtctttta ttcgggggt tcccgcaaa 
      cgtctgcct gatggtttg acacgacgt ggcgatatt 
      aatatttta ttattgggg gatcccgga actgccaag 
      tcgcaatta ttgaagttt gtggaacgg gtggccccc 
      attggagtg tacacttct ggcaaaggg agctccgct 
      gctgggtta acggcgtcg gtaatgcgg gaaccttcc 
      tccaggaat tttgtcatg gaaggaggt gcgatggtt 
      ttggccgat ggtggagtt gtgtgtatt gatgagttt 
      gataaaatg agggaggac gatcgagtt gccattcac 
      gaagcaatg gaacagcaa acgatttcg atcgcgaaa 
      gcggggatt accacaact ttgaattcg cgttgttcc 
      gttctagcg gccgctaat agcattttc gggcgttgg 
      gatgagaca aaaggagag gaaaatatt gattttatg 
      ccgacgatt ctctcacgt tttgatatg attttcatt 
      gtgaaggat gaacacgac caacagcgc gatatgact 
      ttagcccgg catatcatg ggcgtgcac atgaacgcg 
      ggcgaagtc accaaccca gggacggtg aactttcgc 
      tcgaattca tcaaaaaat acatcagat attgcagga 
      cccgttgtg gcccccgtt tga
    mRNA sequence
    • >TC034773 RA
      ccaatttac ttccgtgta acaacaacg ctttattcg 
      ccacaaaat cggtgtttt atacgaaaa tggaaggtt 
      tcgatgatc caggagtgt tttacagtg ataatttca 
      cggttgagg acaaccaaa gcgaccccc aaatcaatt 
      tacaaggtg ttaagaaaa agttcaagg agttcatcc 
      gacaatacc acaccgaca atttcagct acaaataca 
      gggacaccc tgaagcgca attacaacc tgcgccaat 
      attacctgg aagtcaaca ttgaagatg ttggcgctt 
      ttgacgaga cgttggccg acaaactat acaaacacc 
      cgtcggaac acttggtta tatttgagg aagcggcca 
      aggaagtgg ccgacgaac tcacagccc cacgtcccg 
      aaggagagg aagcagttg aagacatac aaattatgc 
      tgtcttcag acgccactc cgacaaact taaggaaca 
      tgaaatcgg acgttgtct caaaactag tgaaaatcc 
      cagggattg tggtcagtg cgtcaggga ttaaagcca 
      aagctacga aaatcgcaa tccagtgcc gttcgtgcc 
      agaacgtaa tccccaatt tacctgtga agcccggga 
      tggaaggct atgttatgc cacgcaaat gcaacacgg 
      agcaagcag gccaggcca agtgcccct tggacccct 
      acttcataa tccctgaca aatgccact gcgtcgatt 
      tccaaatcc tcaaattgc aagaactac cagacggaa 
      tccctcaag gggaaatcc cccgacacc tcaccctct 
      actgcgacc gctacttgt gcgagaaag tggtccccg 
      ggaaccgaa tcttcgtct tggggattt actcgatca 
      agaaaatcg caaaagtgg gccggcgtg atgaccgtg 
      agcgcggca caacgggcg tccgagccc cgtatattc 
      gcgtcgtgg gggtgcaac tcgacactg aaggtgtcg 
      gtggttccc cgacttctg tcgtgaccg ccgaagaag 
      aaaacatgt ttcggcgaa tggccgatt cgcccaata 
      tttacgaac gaatcgcca atagcatcg caccgtcga 
      ttttcggag ctcttgata ttaagaaag ccatagctt 
      gtcttttat tcgggggtt cccgcaaac gtctgcctg 
      atggtttga cacgacgtg gcgatatta atattttat 
      tattggggg atcccggaa ctgccaagt cgcaattat 
      tgaagtttg tggaacggg tggccccca ttggagtgt 
      acacttctg gcaaaggga gctccgctg ctgggttaa 
      cggcgtcgg taatgcggg aaccttcct ccaggaatt 
      ttgtcatgg aaggaggtg cgatggttt tggccgatg 
      gtggagttg tgtgtattg atgagtttg ataaaatga 
      gggaggacg atcgagttg ccattcacg aagcaatgg 
      aacagcaaa cgatttcga tcgcgaaag cggggatta 
      ccacaactt tgaattcgc gttgttccg ttctagcgg 
      ccgctaata gcattttcg ggcgttggg atgagacaa 
      aaggagagg aaaatattg attttatgc cgacgattc 
      tctcacgtt ttgatatga ttttcattg tgaaggatg 
      aacacgacc aacagcgcg atatgactt tagcccggc 
      atatcatgg gcgtgcaca tgaacgcgg gcgaagtca 
      ccaacccag ggacggtga actttcgct cgaattcat 
      caaaaaata catcagata ttgcaggac ccgttgtgg 
      cccccgttt gaacgccga agccggcga caagctcaa 
      gcgccgcta cgtcatgat gcgttccgg tgcccacca 
      gcacgagaa agaggtcga caaacgcaa cagcatccc 
      tattacggt ccgtcaact cgaggcagt aattcgtat 
      tgcggaaag tttagccaa aatgcaact ccttccatt 
      cgctactga aacccacat caatgaagc tttacgcct 
      gtttcaagt ctcaacact ggacgctgc catgagtgg 
      tggcttagc tggtcggta ttacagtta actgtaatt 
      taaccaagt actataata atttgccag gtggtgaag 
      ggttcacga ctgaagaag agcacgaaa tgttgatcc 
      gcatcgaaa aacagctaa aacgccggt ttgctatcg 
      gtactcaag tctcccacc agaccatca ttcaggact 
      tcacccaac agcagtacc ctgaacaag ccataaaca 
      aagtcatct atacgatga tcaggcgtg gacaattac 
      agcacagga tgcaacgaa aaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY