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Gene information
General information
TC033585
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009622
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3341856..3343220
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033585
    iBeetle screen iB_09206

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber potentially sometimesfused - Number of animals showing the phenotype: 1

    vitellogenic egg chamber late sometimesamount increased - Number of animals showing the phenotype: 1

    vitellogenic egg chamber amount decreased - Number of animals showing the phenotype: 1
    vitellogenic egg chamber sometimes potentiallydegenerated - Number of animals showing the phenotype: 2

    previtellogenic egg chamber sometimesnot present - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033585 RA
      MGLFTPIFV AFCASLAHS QLDFEFPKN GHTNNWAVL 
      VDTSRFWFN YRHVANVLS IYRSVKRLG IPDSQIILM 
      IADDMACNP RNPRPATVF NNANQHINV YGDDVEVDY 
      RGYEVTVEN FVRLLTGRL PPGTPRSKQ LLTDEGSNV 
      LIYLTGHGG DGFLKFQDS EEITSQEMA DALEQMWQK 
      QRYHEIFFM IDTCQAASM YERFYSPNI LAVGSSLVG 
      EDSLSHHVD SSIGVYIID RYTFFALEF LENVDQHTK 
      KTMGEFLNV CPKRVCIST VGVRRDLFA RNPHQVPIT 
      DFFGSVRPV EVISNVTLV PLVTNETKT KPVAQPTKK 
      YRYIPPLVN FNSIV
    CDS sequence
    • >TC033585 RA
      atggggctg tttaccccc atttttgtg gccttttgc 
      gcctcgctg gcccactca cagctcgat tttgaattt 
      ccgaaaaat gggcacacg aataattgg gcggttttg 
      gtcgatacg agccgtttc tggttcaat taccggcac 
      gtggccaac gtgctttcg atctatcgt agcgtgaaa 
      cgcctggga atccccgac agccaaata atattaatg 
      atagccgac gatatggcg tgcaacccc cgaaatccg 
      cgccctgcg actgttttt aacaacgca aatcagcac 
      attaatgtg tacggggat gacgttgag gttgactat 
      cgggggtat gaggtcacg gttgagaat ttcgtgcgg 
      ttgttgacg gggaggttg cccccgggg acgccccgg 
      tccaagcaa ttgttgact gatgaaggg agcaacgtt 
      ttgatttat ttaacgggg catgggggc gacggcttt 
      ttgaaattt caggattcg gaggaaata acgagtcag 
      gagatggcc gatgctttg gagcaaatg tggcaaaaa 
      cagagatat cacgaaatt tttttcatg attgatacg 
      tgccaagcc gcctcgatg tatgagcgg ttctactcg 
      cctaatatt ctagccgtg gggagcagt ctcgtgggg 
      gaagactca ctttcgcat cacgttgac tcgtccatt 
      ggtgtttac ataattgat cgttacacg ttttttgca 
      cttgagttt ttggaaaac gtcgatcag catacgaaa 
      aaaacgatg ggcgaattt ttaaatgtt tgtccgaaa 
      cgagtttgt atttcaaca gttggggtc aggcgcgat 
      ttattcgca aggaacccg catcaagtc cccataact 
      gactttttt gggtctgtg aggcctgtt gaggtcatc 
      tcgaatgtt acacttgtc cctctagtc acaaatgag 
      acaaagact aagccagtg gctcagccg acgaagaaa 
      tatcgttac attccgcct ttagttaat tttaattcg 
      attgtgtaa
    mRNA sequence
    • >TC033585 RA
      aaaaaactt cgataattg cagattttg cccaatttt 
      tggaaaatt tgcaagctt ttaagaatt tttattcaa 
      aattttgct caacaaatc accagattt aggaataaa 
      atcactaga ctttggcct aacctcatt ttttgcgcc 
      accaaacta accgggaat ggggctgtt tacccccat 
      ttttgtggc cttttgcgc ctcgctggc ccactcaca 
      gctcgattt tgaatttcc gaaaaatgg gcacacgaa 
      taattgggc ggttttggt cgatacgag ccgtttctg 
      gttcaatta ccggcacgt ggccaacgt gctttcgat 
      ctatcgtag cgtgaaacg cctgggaat ccccgacag 
      ccaaataat attaatgat agccgacga tatggcgtg 
      caacccccg aaatccgcg ccctgcgac tgtttttaa 
      caacgcaaa tcagcacat taatgtgta cggggatga 
      cgttgaggt tgactatcg ggggtatga ggtcacggt 
      tgagaattt cgtgcggtt gttgacggg gaggttgcc 
      cccggggac gccccggtc caagcaatt gttgactga 
      tgaagggag caacgtttt gatttattt aacggggca 
      tgggggcga cggcttttt gaaatttca ggattcgga 
      ggaaataac gagtcagga gatggccga tgctttgga 
      gcaaatgtg gcaaaaaca gagatatca cgaaatttt 
      tttcatgat tgatacgtg ccaagccgc ctcgatgta 
      tgagcggtt ctactcgcc taatattct agccgtggg 
      gagcagtct cgtggggga agactcact ttcgcatca 
      cgttgactc gtccattgg tgtttacat aattgatcg 
      ttacacgtt ttttgcact tgagttttt ggaaaacgt 
      cgatcagca tacgaaaaa aacgatggg cgaattttt 
      aaatgtttg tccgaaacg agtttgtat ttcaacagt 
      tggggtcag gcgcgattt attcgcaag gaacccgca 
      tcaagtccc cataactga cttttttgg gtctgtgag 
      gcctgttga ggtcatctc gaatgttac acttgtccc 
      tctagtcac aaatgagac aaagactaa gccagtggc 
      tcagccgac gaagaaata tcgttacat tccgccttt 
      agttaattt taattcgat tgtgtaatt ttttgaata 
      tttttttgt gaaaaaaaa agaaataaa acaaagttt 
      aaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY