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Gene information
General information
TC009252 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2481847..2485914
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009252
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09198

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009252 RA
      MVGTQFLPR RGTCRMEKW FNGSKFWLF GNQLQLLPK 
      RTRTSSKVM ERYEKLAKL GEGSYGIVY KCRNRDTGE 
      IVAIKKFAE SEDDPLIRK IALREIRLL KNLKHPNLV 
      NLLEVFRRK RRLHLVFEF CERTVLNEL ERYPRGCPH 
      LLTQQIIWQ TLQAVAYCH HHGCIHRDI KPENILLTG 
      SGVVKLCDF GFARMLSPG ENYTDYVAT RWYRAPELL 
      VGDTQYGTP VDVWAIGCV LAELVKGEA LWPGKSDVD 
      QLYLIRSTV GDLLQRHMQ AFKNNDFFQ GVILPVPQQ 
      MVPLEVKLP MCNQTTIDF LKKCLDKDP LKRWTCDML 
      LTHDYFENF NFKVDETDS QNFEKLSRD RSRNSLNGT 
      SLPQLTTNK PISPAKQNN NQRSKIDHL PTI
    CDS sequence
    • >TC009252 RA
      atggtaggc acccagttc ttgccgagg cgaggaacg 
      tgtcggatg gagaaatgg tttaatggt agtaaattt 
      tggttattc ggaaaccaa ctgcagcta ctccccaaa 
      aggacacgc acctcgtcc aaagtcatg gaacgctac 
      gagaaattg gcaaaattg ggtgaaggg agctacggc 
      attgtgtac aaatgtcgc aaccgcgac acgggggaa 
      atagtggcg atcaaaaaa ttcgcggaa agcgaggac 
      gaccccctg atccgcaaa attgctctt cgagaaata 
      agactactc aaaaatttg aagcatcca aacctggta 
      aaccttctg gaagtgttt cggcggaag cgccgcctg 
      caccttgtg ttcgaattt tgcgaacgc acggtttta 
      aacgagctg gagcgctac cccaggggg tgcccccat 
      ctgctcacc cagcagatc atctggcag acgctccag 
      gcggtggct tactgccac caccacggg tgcatccac 
      cgggacatc aagccggag aatatcctc ctgacgggc 
      tccggagtc gtgaaactg tgcgatttt ggcttcgcc 
      cggatgctg agtccgggc gagaactac acggactac 
      gtggcgacg cgctggtac cgggccccg gagctgctg 
      gtcggggac acccagtac gggactccg gtggacgtc 
      tgggccata ggttgcgtt ctggccgaa ttggtgaaa 
      ggcgaggcg ctctggccg gggaaatcc gatgtggat 
      caactgtac cttatcaga tcgacggtt ggtgacctt 
      ttgcagagg cacatgcaa gcgttcaag aataacgat 
      tttttccaa ggggttatt ctgccggtg ccgcagcaa 
      atggtgccg ctggaggtc aagctgccc atgtgcaac 
      cagaccacg atagatttt ttaaaaaaa tgtctggat 
      aaagaccct ctgaaacgg tggacctgt gatatgctt 
      ctcacgcac gactatttc gaaaatttt aatttcaaa 
      gtggatgaa accgactcg caaaatttc gagaaattg 
      tcgagggac aggtcacga aattcactc aatggtacc 
      tccttacct caactaacg acaaataaa ccgataagt 
      ccggcgaaa caaaataat aatcaacga tccaaaatc 
      gaccattta ccgactata tga
    mRNA sequence
    • >TC009252 RA
      taaatatca acaatggta ggcacccag ttcttgccg 
      aggcgagga acgtgtcgg atggagaaa tggtttaat 
      ggtagtaaa ttttggtta ttcggaaac caactgcag 
      ctactcccc aaaaggaca cgcacctcg tccaaagtc 
      atggaacgc tacgagaaa ttggcaaaa ttgggtgaa 
      gggagctac ggcattgtg tacaaatgt cgcaaccgc 
      gacacgggg gaaatagtg gcgatcaaa aaattcgcg 
      gaaagcgag gacgacccc ctgatccgc aaaattgct 
      cttcgagaa ataagacta ctcaaaaat ttgaagcat 
      ccaaacctg gtaaacctt ctggaagtg tttcggcgg 
      aagcgccgc ctgcacctt gtgttcgaa ttttgcgaa 
      cgcacggtt ttaaacgag ctggagcgc taccccagg 
      gggtgcccc catctgctc acccagcag atcatctgg 
      cagacgctc caggcggtg gcttactgc caccaccac 
      gggtgcatc caccgggac atcaagccg gagaatatc 
      ctcctgacg ggctccgga gtcgtgaaa ctgtgcgat 
      tttggcttc gcccggatg ctgagtccg ggcgagaac 
      tacacggac tacgtggcg acgcgctgg taccgggcc 
      ccggagctg ctggtcggg gacacccag tacgggact 
      ccggtggac gtctgggcc ataggttgc gttctggcc 
      gaattggtg aaaggcgag gcgctctgg ccggggaaa 
      tccgatgtg gatcaactg taccttatc agatcgacg 
      gttggtgac cttttgcag aggcacatg caagcgttc 
      aagaataac gattttttc caaggggtt attctgccg 
      gtgccgcag caaatggtg ccgctggag gtcaagctg 
      cccatgtgc aaccagacc acgatagat tttttaaaa 
      aaatgtctg gataaagac cctctgaaa cggtggacc 
      tgtgatatg cttctcacg cacgactat ttcgaaaat 
      tttaatttc aaagtggat gaaaccgac tcgcaaaat 
      ttcgagaaa ttgtcgagg gacaggtca cgaaattca 
      ctcaatggt acctcctta cctcaacta acgacaaat 
      aaaccgata agtccggcg aaacaaaat aataatcaa 
      cgatccaaa atcgaccat ttaccgact atatgacaa 
      agactttcg tttttttcg aagtaaatc atagcaaat 
      ctctatttt gtgtatttg aaaattaaa gtgttatat 
      tttagaggt agaattaag cgtaatcgt tgcattgta 
      cttatgttg agaggaata aaaaatgag tccaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY