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Gene information
General information
TC009283 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1925657..1926853
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009283
    iBeetle screen iB_09194

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009283 RA
      MELVPTKIL VAVLFGVLR FFFGILPVK IYKLLLHWE 
GQDDSQHFI NKKRHQQVT CTLALCQSF GGGVLFATC 
MLHMMPEVY KSVEELKRL GNVNTDYPL SQMTISVGF 
FLVYFIEEL SHWFVTHVP DDACERISR SATPTSNKV 
APKSAFTEE KKPIPQTPF IVDEYEKKP PDEPDTYSL 
NAEEIENEK NQKNIDLDS ELERVESAK SSVEKAVKT 
KQQIMRYVL VVVALSFHA MFEGLAIGL QHSIANIWY 
LFVAVSIHS ATILFYISL ELVLAKTEL RRILTHVAI 
LSVTSPAGV LLGLLITQK ANMNTQAKS TAVVLLEGL 
SAGTILYIT FFEVLNREK ERRAWTIRR GVCILGGFV 
LMALLQCAE MYIE
    CDS sequence
    • >TC009283 RA
      atggagttg gtccccacg aaaatccta gtggctgtc 
ctcttcgga gtcctccgc tttttcttc ggcattctc 
cccgttaaa atctacaag ttgctgctg cactgggag 
ggccaggac gactcccag cacttcatc aacaagaag 
cgccaccag caggtcacc tgcaccctc gccctgtgc 
cagtccttc ggcggcggc gtcctcttc gccacctgc 
atgctccac atgatgccc gaggtgtac aaatcggtc 
gaggagctc aaacgcctc ggcaacgtc aacaccgac 
taccccctc tcgcagatg accatctcc gtcggcttt 
ttcctagtg tacttcatc gaggagctg tcccactgg 
ttcgtgacc cacgtcccg gacgacgcc tgcgagaga 
atctcgcgc tcggcgacc cccacgtcc aacaaagtg 
gccccgaag agtgccttc accgaggag aagaagccc 
atcccccaa accccgttc attgtggac gagtacgag 
aagaaacca ccggacgaa cccgacact tacagtctg 
aatgcggag gagattgaa aatgagaaa aaccagaaa 
aatatcgac ctggactcg gagctggag cgcgtggag 
tcggccaag tcctcggtg gagaaggcg gtcaagacc 
aagcagcaa atcatgcgc tatgtcctg gtggtggtg 
gctttgtcc ttccatgcc atgttcgaa ggcctggcg 
attgggctc cagcacagc atcgccaat atttggtac 
ctgtttgtg gccgtttcc attcactca gccacgatt 
ttattttac attagtctc gagctggtt ttggccaaa 
actgagctg aggcggatt ctcacccac gttgcgatt 
ttgtcggtt actagtccg gctggggtc ctcttgggg 
cttcttatt acgcagaaa gctaacatg aatacgcaa 
gccaagtcc acggcagtg gtgctcttg gaagggctg 
tcggctggg acgattttg tacattacg ttttttgaa 
gttttgaat cgggagaag gagcggaga gcgtggact 
ataagacgt ggagtgtgc attttaggg gggtttgtg 
ctcatggcc ctgttacag tgtgcggaa atgtatata 
gagtga
    mRNA sequence
    • >TC009283 RA
      aaacctccc tcgctgtga gtcagtcaa gccgcgcca 
ctcgaccat gcagcacca tggagttgg tccccacga 
aaatcctag tggctgtcc tcttcggag tcctccgct 
ttttcttcg gcattctcc ccgttaaaa tctacaagt 
tgctgctgc actgggagg gccaggacg actcccagc 
acttcatca acaagaagc gccaccagc aggtcacct 
gcaccctcg ccctgtgcc agtccttcg gcggcggcg 
tcctcttcg ccacctgca tgctccaca tgatgcccg 
aggtgtaca aatcggtcg aggagctca aacgcctcg 
gcaacgtca acaccgact accccctct cgcagatga 
ccatctccg tcggctttt tcctagtgt acttcatcg 
aggagctgt cccactggt tcgtgaccc acgtcccgg 
acgacgcct gcgagagaa tctcgcgct cggcgaccc 
ccacgtcca acaaagtgg ccccgaaga gtgccttca 
ccgaggaga agaagccca tcccccaaa ccccgttca 
ttgtggacg agtacgaga agaaaccac cggacgaac 
ccgacactt acagtctga atgcggagg agattgaaa 
atgagaaaa accagaaaa atatcgacc tggactcgg 
agctggagc gcgtggagt cggccaagt cctcggtgg 
agaaggcgg tcaagacca agcagcaaa tcatgcgct 
atgtcctgg tggtggtgg ctttgtcct tccatgcca 
tgttcgaag gcctggcga ttgggctcc agcacagca 
tcgccaata tttggtacc tgtttgtgg ccgtttcca 
ttcactcag ccacgattt tattttaca ttagtctcg 
agctggttt tggccaaaa ctgagctga ggcggattc 
tcacccacg ttgcgattt tgtcggtta ctagtccgg 
ctggggtcc tcttggggc ttcttatta cgcagaaag 
ctaacatga atacgcaag ccaagtcca cggcagtgg 
tgctcttgg aagggctgt cggctggga cgattttgt 
acattacgt tttttgaag ttttgaatc gggagaagg 
agcggagag cgtggacta taagacgtg gagtgtgca 
ttttagggg ggtttgtgc tcatggccc tgttacagt 
gtgcggaaa tgtatatag agtgataaa taaattgta 
tttttttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY