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Gene information
General information
TC015857 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9637684..9639336
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015857
    iBeetle screen iB_09182

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015857 RA
      MSSQQHFTS IEKILSKLP PDDYQNIRN VLYGKPTSK 
      LDLSDAETK ANDYDLELV SCRFEASPE QLRPPKIVR 
      VGIFQHKLP LPPNTPIKE MRDGLYKLA QNAIETAAK 
      GGVNIFCFQ EAWNMPFAF CTREKQPWC EYAEVAEFG 
      PTTIFLQQL AKRYNMVVI SPILERDEV HGQTIWNTA 
      VVIDNYGDF LGKHRKNHI PRVGDFNES TYYFEGNTG 
      HPVFQTEFG KIAINICYG RHHPLNWLM FGLNGAEIV 
      FNPSATVGN LSEPLWGIE ARNAAIANS YFTCAINRV 
      GTEIFETEF TSGDGKPAH KEFGHFYGS SYIAAPDGS 
      RSQGLSRDK TGLLIAELD LNLCRQVRD HWSFQMTQR 
      LDMYAELLA KVVQPDFKP QIIKK
    CDS sequence
    • >TC015857 RA
      atgtcttct caacaacac ttcaccagc attgaaaaa 
      atcctatcg aaactcccc cctgatgat taccaaaac 
      atccgaaac gtgctatac ggcaaaccg acaagcaaa 
      ctcgattta tcggatgcc gaaacaaaa gcaaacgat 
      tatgacctt gagctggtc tcttgtcgc ttcgaggcc 
      tcacctgaa cagctaaga ccaccgaaa atcgtccgt 
      gttggcatt ttccaacac aaactaccc cttcctccc 
      aacactccc atcaaggag atgcgagac gggctttac 
      aaattggcc caaaacgca atcgagacg gctgcgaaa 
      ggaggagtc aatatattt tgtttccag gaggcttgg 
      aatatgccg tttgcgttc tgcacgcgc gaaaaacaa 
      ccgtggtgc gaatacgca gaggtggcc gagttcggg 
      cctactacg atttttttg caacaattg gcgaaacgt 
      tacaacatg gttgtaatt tctccaatt ttggaacgt 
      gacgaagtg catggccaa acgatttgg aacacggcc 
      gttgttatt gacaattat ggcgatttt ttggggaaa 
      caccgcaaa aatcacata cccagggtt ggggatttt 
      aacgaatcg acttactat tttgaggga aataccggg 
      caccctgtt ttccagacg gaatttggc aaaatcgct 
      ataaatatc tgttacggg cgccaccac ccgctcaat 
      tggttaatg tttggccta aatggtgcc gaaatcgtc 
      ttcaatccg tcggcaact gttggtaac ttaagcgag 
      cctttgtgg ggcatcgag gcccgaaat gcggccatt 
      gccaatagc tacttcacg tgtgctatt aatcgcgtt 
      ggcactgag attttcgaa actgaattc acttccggt 
      gatggcaaa cccgcccat aaagagttc ggacatttc 
      tatggctca agttacata gccgccccc gatggctca 
      agatcacaa ggattatct cgggacaaa accgggtta 
      ttgatagcc gaactggac ttgaatctg tgtcggcaa 
      gttagggac cattggagt ttccagatg acgcaaaga 
      ttggatatg tatgccgaa ctcttggct aaagtcgtt 
      cagcccgat tttaaacca caaattatt aagaaataa
    mRNA sequence
    • >TC015857 RA
      cgataaata aacattaat tctatttta aaaattctg 
      gcatttcaa catgtcttc tcaacaaca cttcaccag 
      cattgaaaa aatcctatc gaaactccc ccctgatga 
      ttaccaaaa catccgaaa cgtgctata cggcaaacc 
      gacaagcaa actcgattt atcggatgc cgaaacaaa 
      agcaaacga ttatgacct tgagctggt ctcttgtcg 
      cttcgaggc ctcacctga acagctaag accaccgaa 
      aatcgtccg tgttggcat tttccaaca caaactacc 
      ccttcctcc caacactcc catcaagga gatgcgaga 
      cgggcttta caaattggc ccaaaacgc aatcgagac 
      ggctgcgaa aggaggagt caatatatt ttgtttcca 
      ggaggcttg gaatatgcc gtttgcgtt ctgcacgcg 
      cgaaaaaca accgtggtg cgaatacgc agaggtggc 
      cgagttcgg gcctactac gattttttt gcaacaatt 
      ggcgaaacg ttacaacat ggttgtaat ttctccaat 
      tttggaacg tgacgaagt gcatggcca aacgatttg 
      gaacacggc cgttgttat tgacaatta tggcgattt 
      tttggggaa acaccgcaa aaatcacat acccagggt 
      tggggattt taacgaatc gacttacta ttttgaggg 
      aaataccgg gcaccctgt tttccagac ggaatttgg 
      caaaatcgc tataaatat ctgttacgg gcgccacca 
      cccgctcaa ttggttaat gtttggcct aaatggtgc 
      cgaaatcgt cttcaatcc gtcggcaac tgttggtaa 
      cttaagcga gcctttgtg gggcatcga ggcccgaaa 
      tgcggccat tgccaatag ctacttcac gtgtgctat 
      taatcgcgt tggcactga gattttcga aactgaatt 
      cacttccgg tgatggcaa acccgccca taaagagtt 
      cggacattt ctatggctc aagttacat agccgcccc 
      cgatggctc aagatcaca aggattatc tcgggacaa 
      aaccgggtt attgatagc cgaactgga cttgaatct 
      gtgtcggca agttaggga ccattggag tttccagat 
      gacgcaaag attggatat gtatgccga actcttggc 
      taaagtcgt tcagcccga ttttaaacc acaaattat 
      taagaaata attcaatca caacgcaaa atctttggc 
      aaatgataa taaataatc gatttttct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY