Status
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Gene information
General information
TC015003 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4935334..4945552
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015003
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09174

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015003 RA
      MEPPGMETI DFPKNPLLC PICHDYFTE PCILSCYHT 
      FCARCLRGR EQDRRLVCP FCRQPTLLK DGSMLPPPD 
      SLMRQLIDI ANSENPPCS NCDKRDRAN MYYCNTCGQ 
      ALCSHCRDN THRAKMFSS HDIVHMTKC TKEPKRCPQ 
      HGEQYIMYS PLQNNMLCV NCFRDLPNE IRAQCVDID 
      TAHSQAAKR LERGQNAIM DLQTSVRDG IIALKSMMD 
      ELRRNMDSE KHTINTFCQ GMQEAMAKT HAAMIMEVQ 
      RQYESKDRI YRSQLLVLG TVMPVLQLH LVLCTTFTA 
      AANKYQFLE LCPSLLERL SAVGQLSQP VRSLQSSTI 
      KTNYRSEFA QCLEPWIGP AAVTQHQSE VAAASRMYD 
      SAPPATKKQ QTALKNKVL EGDSSFSAH CRSFESQIR 
      DLNQQFNVV KEKVSDLHR DITALRKAQ APPLAARYD 
      MLMRECLHL EQNLERQQQ ELDRMAENF DASWEEQLW 
      RLRVEQEVF SCQRADVMT LRNELKHIS QLVGQLEPY 
      IRSLTPAQQ SESAAEHSQ QMQTLLDHF NVLQQSDAS 
      LGKIPRSRS RVLGQLFEK VRPNIQERE RSKSAGQTD 
      KPLSTPLKP KKPFIPSTH QMNIERLEA HLKEKIHEM 
      VCRRQSSST PQSKSDPET ERKLSKTDL DSISKLGTK 
      QRIMYKKIG KSCDKINTC SKSDGDVKK RAESDYQRI 
      SEATSQSVK ALVHKETRS SPSSPKCKG KPKPPPKQR 
      KTPKVYPYS DGEDNVFYS LHDSADSMS TSSRRSSFE 
      GPDKTLVVV INKRSNNMT MAQKQRSWE TFPPKRRHH 
      MCHKLSAPN PMPLRKADS FEGHEEAVK SLVAAVQET 
      RRKPKGGN
    CDS sequence
    • >TC015003 RA
      atggaacca ccagggatg gagactata gacttcccc 
      aaaaacccc ctcttgtgc cccatttgc cacgactat 
      ttcacagag ccgtgcatt ctgagctgt taccacacg 
      ttctgtgcg cgctgtttg cgggggcgc gagcaggac 
      cggcgcctc gtgtgcccc ttttgcagg caaccgacg 
      ctgctcaag gacggctcc atgctgccg ccccccgac 
      agcctcatg cggcagctc attgatatt gcgaatagt 
      gagaacccc ccgtgttca aattgcgat aaaagggac 
      agggccaac atgtactac tgcaatacg tgcgggcaa 
      gcgctgtgt tcccactgc cgtgacaat acacaccgt 
      gcgaaaatg ttctcaagt catgatatc gtgcatatg 
      acgaagtgc accaaagag ccgaaacgg tgtccgcaa 
      catggagaa cagtacatt atgtattcg ccgcttcag 
      aacaatatg ctgtgtgtt aattgtttc cgggattta 
      ccgaatgaa ataagagca cagtgtgtt gatattgac 
      actgcccat agtcaagct gcgaagcga ttggagcgc 
      gggcaaaat gccatcatg gatttgcag acctcagtc 
      cgcgacggc ataatcgcc ctcaaaagc atgatggac 
      gaattacgc cgtaacatg gacagcgaa aagcacaca 
      attaacaca ttctgccaa ggaatgcaa gaagcaatg 
      gccaaaacg cacgccgcc atgataatg gaagtgcaa 
      cgacaatac gaaagcaaa gaccgaatc taccgcagc 
      caacttcta gtcttaggc accgtgatg cccgtttta 
      caactccac ctcgttctg tgcacaact ttcactgcc 
      gccgccaac aagtaccag ttcctcgag ctgtgccct 
      tcgctcctc gagcgcctc tcggccgtc ggccagcta 
      agccagccg gtgcgttcg ctgcaaagc tccaccatc 
      aagaccaac taccgcagc gagttcgcc caatgcctg 
      gagccctgg atcggcccc gcggccgtc acccagcac 
      cagtccgaa gtggccgca gcctcgcgc atgtacgac 
      agcgcgccc cctgccacc aaaaagcag caaacggcg 
      ctcaagaac aaggtgctg gagggcgac agctcgttt 
      tcggcgcat tgtcgctcg ttcgagtcg caaatccgc 
      gacctgaac cagcagttc aacgtggtt aaggagaag 
      gtgagcgat ttgcatcgg gacataacg gcgctgagg 
      aaggcgcag gcgccgccg ctggccgcg cggtacgat 
      atgctcatg agggagtgt ctgcatctg gagcagaat 
      ctggagcgg cagcagcag gagctggac aggatggcg 
      gagaatttc gacgctagt tgggaggag cagctgtgg 
      aggttgagg gtggagcag gaggtgttc agttgtcag 
      agggccgat gttatgacg ttgaggaac gagttgaaa 
      catataagt caactggtg gggcaactt gagccctat 
      attaggagt cttacgcca gcacagcag agtgagagt 
      gcggctgaa catagtcaa cagatgcaa acgcttctt 
      gaccatttt aatgtttta caacaatcc gacgctagt 
      ttgggcaaa atcccgcgc tcgcggtcg cgcgtttta 
      ggccaactc ttcgaaaaa gtccgtcca aatatccag 
      gaacgcgaa cggagcaaa tccgccggc cagacggac 
      aagccctta agcacccca ctcaagccg aagaagccc 
      ttcatcccc tccacccac cagatgaac atcgagcgt 
      ctggaggcg cacctgaag gagaaaatc cacgaaatg 
      gtgtgccgc cgccagtcg tcctccacc ccccagtcc 
      aagtccgac cccgagacc gagcgcaag ctcagcaaa 
      accgacctg gactccatc agcaagctc ggcaccaag 
      cagcgcatc atgtacaag aaaatcggc aaaagctgc 
      gacaaaatc aacacctgc agcaagtcg gacggcgac 
      gtcaagaag cgcgcggag tcggactac cagcgcatc 
      tccgaggcc accagccag agcgtcaag gcgctggtg 
      cacaaggag acgcgctcc agccccagc agccccaag 
      tgcaagggc aagccgaag ccgccgccc aagcagcgg 
      aaaaccccc aaggtttat ccgtacagt gatggcgag 
      gacaacgtt ttctactcc ctgcacgac tccgccgac 
      tccatgagc acgtccagc cggaggtcg agcttcgaa 
      gggccggac aagaccctg gtggtggtg atcaacaag 
      cggagcaat aacatgacg atggcccag aagcagcgc 
      agttgggag acgtttccg ccgaagcgg cgccaccac 
      atgtgccac aagctctcg gcgcccaac cccatgccc 
      ttgcggaag gcggacagt ttcgagggg cacgaggag 
      gcggttaag agtttggtg gcggcggtg caagagact 
      agacggaag ccgaaaggg gggaattaa
    mRNA sequence
    • >TC015003 RA
      gatggaaat aaaaaacat taatggcaa ccctcttgt 
      agctccaag gggttgaaa ttgcacttt tcgggatta 
      tttacttcg tcaggtgag tgtttgtac tctgaacca 
      gcaaaaccc cttctagtc actgtaatt tcgaaatgg 
      aaccaccag ggatggaga ctatagact tccccaaaa 
      accccctct tgtgcccca tttgccacg actatttca 
      cagagccgt gcattctga gctgttacc acacgttct 
      gtgcgcgct gtttgcggg ggcgcgagc aggaccggc 
      gcctcgtgt gcccctttt gcaggcaac cgacgctgc 
      tcaaggacg gctccatgc tgccgcccc ccgacagcc 
      tcatgcggc agctcattg atattgcga atagtgaga 
      accccccgt gttcaaatt gcgataaaa gggacaggg 
      ccaacatgt actactgca atacgtgcg ggcaagcgc 
      tgtgttccc actgccgtg acaatacac accgtgcga 
      aaatgttct caagtcatg atatcgtgc atatgacga 
      agtgcacca aagagccga aacggtgtc cgcaacatg 
      gagaacagt acattatgt attcgccgc ttcagaaca 
      atatgctgt gtgttaatt gtttccggg atttaccga 
      atgaaataa gagcacagt gtgttgata ttgacactg 
      cccatagtc aagctgcga agcgattgg agcgcgggc 
      aaaatgcca tcatggatt tgcagacct cagtccgcg 
      acggcataa tcgccctca aaagcatga tggacgaat 
      tacgccgta acatggaca gcgaaaagc acacaatta 
      acacattct gccaaggaa tgcaagaag caatggcca 
      aaacgcacg ccgccatga taatggaag tgcaacgac 
      aatacgaaa gcaaagacc gaatctacc gcagccaac 
      ttctagtct taggcaccg tgatgcccg ttttacaac 
      tccacctcg ttctgtgca caactttca ctgccgccg 
      ccaacaagt accagttcc tcgagctgt gcccttcgc 
      tcctcgagc gcctctcgg ccgtcggcc agctaagcc 
      agccggtgc gttcgctgc aaagctcca ccatcaaga 
      ccaactacc gcagcgagt tcgcccaat gcctggagc 
      cctggatcg gccccgcgg ccgtcaccc agcaccagt 
      ccgaagtgg ccgcagcct cgcgcatgt acgacagcg 
      cgccccctg ccaccaaaa agcagcaaa cggcgctca 
      agaacaagg tgctggagg gcgacagct cgttttcgg 
      cgcattgtc gctcgttcg agtcgcaaa tccgcgacc 
      tgaaccagc agttcaacg tggttaagg agaaggtga 
      gcgatttgc atcgggaca taacggcgc tgaggaagg 
      cgcaggcgc cgccgctgg ccgcgcggt acgatatgc 
      tcatgaggg agtgtctgc atctggagc agaatctgg 
      agcggcagc agcaggagc tggacagga tggcggaga 
      atttcgacg ctagttggg aggagcagc tgtggaggt 
      tgagggtgg agcaggagg tgttcagtt gtcagaggg 
      ccgatgtta tgacgttga ggaacgagt tgaaacata 
      taagtcaac tggtggggc aacttgagc cctatatta 
      ggagtctta cgccagcac agcagagtg agagtgcgg 
      ctgaacata gtcaacaga tgcaaacgc ttcttgacc 
      attttaatg ttttacaac aatccgacg ctagtttgg 
      gcaaaatcc cgcgctcgc ggtcgcgcg ttttaggcc 
      aactcttcg aaaaagtcc gtccaaata tccaggaac 
      gcgaacgga gcaaatccg ccggccaga cggacaagc 
      ccttaagca ccccactca agccgaaga agcccttca 
      tcccctcca cccaccaga tgaacatcg agcgtctgg 
      aggcgcacc tgaaggaga aaatccacg aaatggtgt 
      gccgccgcc agtcgtcct ccacccccc agtccaagt 
      ccgaccccg agaccgagc gcaagctca gcaaaaccg 
      acctggact ccatcagca agctcggca ccaagcagc 
      gcatcatgt acaagaaaa tcggcaaaa gctgcgaca 
      aaatcaaca cctgcagca agtcggacg gcgacgtca 
      agaagcgcg cggagtcgg actaccagc gcatctccg 
      aggccacca gccagagcg tcaaggcgc tggtgcaca 
      aggagacgc gctccagcc ccagcagcc ccaagtgca 
      agggcaagc cgaagccgc cgcccaagc agcggaaaa 
      cccccaagg tttatccgt acagtgatg gcgaggaca 
      acgttttct actccctgc acgactccg ccgactcca 
      tgagcacgt ccagccgga ggtcgagct tcgaagggc 
      cggacaaga ccctggtgg tggtgatca acaagcgga 
      gcaataaca tgacgatgg cccagaagc agcgcagtt 
      gggagacgt ttccgccga agcggcgcc accacatgt 
      gccacaagc tctcggcgc ccaacccca tgcccttgc 
      ggaaggcgg acagtttcg aggggcacg aggaggcgg 
      ttaagagtt tggtggcgg cggtgcaag agactagac 
      ggaagccga aagggggga attaacccg agtttgttt 
      acgaggggt ttttgttgt attatgttg gaaagtgat 
      cgttggcct aatttatta ttaatgttg ctactgttc 
      gactagctt agaattgtg tatcaagtg actagatta 
      ggaatttcg agtttgtga cgaatttgg aaatatatt 
      gggcgaaat aaacgttga attatttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY