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Gene information
General information
TC015001 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4907674..4912130
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015001
    iBeetle screen iB_09173

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015001 RA
      MKVLAKALQ ALSKIGDEL FIEAKTDGL AFITLNSSK 
      TVCSRFTFQ EAFFSSYEV NQNDSTEDI SCKIHMKIF 
      LPLFKGNLE KKLEYFKVE YLVDSDFII FKMKYKCDD 
      IVMVHKLRL MDTETLSIG VTTNSGCNN VSASSSFYN 
      QLLSMFNLT DDEVTFEIT KAKVVARNY CLGTPCRPK 
      MMRTQINLN STEFLTYFI TKTSSINFS LKPFRTLVH 
      FAETFNLNV DLNFEIGGK PLSMVLKNP TFEVSFIVA 
      TLDPYSDTN SSIATVSSP KIATKKPPK ITDEADDLT 
      SKESSFLEL MKQSENVND IDVIPKSPE SPRSKKAKT 
      VFGRCYDPT FHETVLGEV LAANSDSE
    CDS sequence
    • >TC015001 RA
      atgaaagtt ttagccaag gcccttcag gccctttct 
      aaaatcggc gacgaattg ttcatagaa gcaaaaact 
      gatggtttg gctttcata actttaaat tcgagcaag 
      acagtttgc tcccgcttt acgttccaa gaggcgttt 
      ttcagtagt tatgaagtg aaccaaaat gattcaact 
      gaagacatc agttgtaag atacacatg aagatattc 
      ctgcccctt tttaaggga aatctagag aaaaaactt 
      gaatatttc aaagttgaa tatttggta gatagcgat 
      tttatcatt tttaaaatg aagtacaaa tgtgacgat 
      attgtgatg gtgcacaag ttacgtctg atggacact 
      gaaacgtta tcaattggt gttaccacc aactcaggg 
      tgcaataac gtatcagct tccagctcg ttttataat 
      caactgctt tcaatgttt aatctcaca gacgatgaa 
      gttacgttt gaaatcaca aaagccaag gttgtggcg 
      cgaaattat tgcttaggc acgccgtgt cgtccaaaa 
      atgatgcga actcaaatc aatttaaac tcaaccgaa 
      tttctgacg tacttcatt acgaaaacc tcatcaatt 
      aatttctct ttgaaacca tttcgtact ctagtccat 
      tttgccgaa acttttaac ctcaacgtg gatttaaat 
      tttgaaatc ggaggaaaa ccgttatct atggtttta 
      aaaaaccca actttcgaa gtcagtttc atcgttgca 
      acgcttgat ccatattcc gacacaaat tcctcaatt 
      gcaactgtg agttcgcct aaaattgcc acgaaaaaa 
      ccgcctaaa attaccgac gaagcggac gatttaacg 
      agtaaagag agtagtttt ctcgagttg atgaaacaa 
      agcgagaat gtgaacgac attgacgtc ataccaaaa 
      tcacccgaa tctccccga agtaagaaa gcgaaaaca 
      gtattcggt cgttgctac gacccgacg tttcacgaa 
      acagtgtta ggggaagtc ctggccgcc aatagcgat 
      tccgagtga
    mRNA sequence
    • >TC015001 RA
      gtcaatttg aaaagttgg ggcttcaga ttaaatgtt 
      cattttgat aaaaacact tattagctt ccaatttat 
      tgactaatg tgcggttat ttggctcga aacaattat 
      ggactgcat aattccggg gacaaacat gaaagtttt 
      agccaaggc ccttcaggc cctttctaa aatcggcga 
      cgaattgtt catagaagc aaaaactga tggtttggc 
      tttcataac tttaaattc gagcaagac agtttgctc 
      ccgctttac gttccaaga ggcgttttt cagtagtta 
      tgaagtgaa ccaaaatga ttcaactga agacatcag 
      ttgtaagat acacatgaa gatattcct gcccctttt 
      taagggaaa tctagagaa aaaacttga atatttcaa 
      agttgaata tttggtaga tagcgattt tatcatttt 
      taaaatgaa gtacaaatg tgacgatat tgtgatggt 
      gcacaagtt acgtctgat ggacactga aacgttatc 
      aattggtgt taccaccaa ctcagggtg caataacgt 
      atcagcttc cagctcgtt ttataatca actgctttc 
      aatgtttaa tctcacaga cgatgaagt tacgtttga 
      aatcacaaa agccaaggt tgtggcgcg aaattattg 
      cttaggcac gccgtgtcg tccaaaaat gatgcgaac 
      tcaaatcaa tttaaactc aaccgaatt tctgacgta 
      cttcattac gaaaacctc atcaattaa tttctcttt 
      gaaaccatt tcgtactct agtccattt tgccgaaac 
      ttttaacct caacgtgga tttaaattt tgaaatcgg 
      aggaaaacc gttatctat ggttttaaa aaacccaac 
      tttcgaagt cagtttcat cgttgcaac gcttgatcc 
      atattccga cacaaattc ctcaattgc aactgtgag 
      ttcgcctaa aattgccac gaaaaaacc gcctaaaat 
      taccgacga agcggacga tttaacgag taaagagag 
      tagttttct cgagttgat gaaacaaag cgagaatgt 
      gaacgacat tgacgtcat accaaaatc acccgaatc 
      tccccgaag taagaaagc gaaaacagt attcggtcg 
      ttgctacga cccgacgtt tcacgaaac agtgttagg 
      ggaagtcct ggccgccaa tagcgattc cgagtgaaa 
      cacacagta gtatattaa ataattact atatttggt 
      acaacatga gataagaag attgatatg ttctattta 
      catttcgtt aaatcatca gataaaaaa gctaagcgg 
      gcctgcttt ggcactgtt cgcaataaa gtcccgctt 
      gaactttgt tacgtcttg atacggata aaatactgg 
      tgaaagatg cttaaaaaa ggcacattt aaacattca 
      tacatgaaa atagtggta agttaagac gttgtaata 
      tttgtattt ggtaatagt tgtttgttg ttaagtgta 
      taaactaca gaatataga attgaattc caccttccc 
      taacttttt ataaaatat atattcata tgtatatta 
      atattttac acaaactat cttttttaa caaaataaa 
      tatcgtatc ttacaaact gttaacact ttagaatcc 
      aaacgttgc gaaaaaaac gccaatgca acaacaata 
      atataaaaa tatgaagca catttaacc aattatttc 
      caaaaactg aaacatttg gcacgcttt gttgggtta 
      atgaaacaa tcacctcgc ccgagacct aacatttta 
      aaaataatg aggtatttt tgatttctc ctccatcta 
      gtacgtaaa atagtgaca ctacatcga gttctactt 
      gttttcaat caacagttg cagttggag caccaaatc 
      tctggccag agacagaac ggctgaagc gttgtgtcg 
      ttcagccat ttctgtaaa aactctcat ttttcgtgc 
      aattgattg aataaactt accgttaaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY