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Gene information
General information
TC015665 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4476484..4478063
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015665
    iBeetle screen iB_09171

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015665 RA
      MTDIYSGHS QFVLLGNNR RRWFDGRTL FIITVITVP 
      VIIFCGIYT IILRSNESL LYPRSPTEV GPGRGAHRA 
      KMYSSRLRK PDDRGLLLI EEDVPKIAD FRLVCYYNF 
      PSADGGLQP ESLDPFLCT HINVAFARV GPNGVQLTR 
      DELRVLQTV VGLKQVNGD LKVLVSVGG AGNDGGYSG 
      MVLNHASRK VFIRSVIDL VRNYSVDGV DLDWEFPNE 
      SPGKDRNQK VHFVQLLEE FRMAINKQA RHKFIVTDK 
      GYFSTLNIN FTAHYWVSK GMAREKIVI GLPAYGHTF 
      RYIAQNQW
    CDS sequence
    • >TC015665 RA
      atgactgat atttattct ggccattct cagttcgtt 
      ttattggga aataacagg agacgatgg ttcgacggg 
      cgcaccctc ttcataatc accgttatc accgtccct 
      gtcataata ttctgtggt atttacacg attatactt 
      agaagcaac gaaagtctc ttgtacccc cgaagcccc 
      accgaagtg gggccaggt cggggcgcc caccgtgcc 
      aaaatgtac agctcccgg ctgcgcaag cccgacgac 
      agggggctg ctgctgatc gaagaggac gtgccaaaa 
      atcgccgat tttaggcta gtttgttac tataatttt 
      ccaagcgcg gacgggggg ctgcagccc gagagcctg 
      gaccccttc ctgtgcacg catatcaac gtggcgttc 
      gcccgtgtg ggccccaat ggggtacag ctgactcgg 
      gacgagctg agggtgctc caaaccgtg gtggggttg 
      aagcaagtc aatggggat ttgaaggtg ttggtgtcc 
      gtgggcggg gctgggaat gatgggggc tacagtggc 
      atggtgtta aatcacgcg agtcggaaa gtttttatt 
      cgatcggtg atcgactta gtgcggaat tatagcgtg 
      gatggggtt gatctcgat tgggagttt ccgaatgaa 
      agtccgggg aaggaccgg aaccagaag gtgcatttc 
      gtgcagctt ttggaggag tttcgaatg gcgattaat 
      aagcaggcg aggcataag tttattgtg acggataag 
      gggtacttt tcaacgctt aatattaat ttcacggca 
      cactattgg gtttccaaa gggatggca agggagaaa 
      attgtgatt ggtttgcct gcttatgga cacactttt 
      cggtacata gctcaaaat caatggtaa
    mRNA sequence
    • >TC015665 RA
      tcaattatt caaatttgg ggaagtgtt tccggtcac 
      gtgacccga tttgtcagt caacaaaaa cctaaccac 
      aaattatga ctgatattt attctggcc attctcagt 
      tcgttttat tgggaaata acaggagac gatggttcg 
      acgggcgca ccctcttca taatcaccg ttatcaccg 
      tccctgtca taatattct gtggtattt acacgatta 
      tacttagaa gcaacgaaa gtctcttgt acccccgaa 
      gccccaccg aagtggggc caggtcggg gcgcccacc 
      gtgccaaaa tgtacagct cccggctgc gcaagcccg 
      acgacaggg ggctgctgc tgatcgaag aggacgtgc 
      caaaaatcg ccgatttta ggctagttt gttactata 
      attttccaa gcgcggacg gggggctgc agcccgaga 
      gcctggacc ccttcctgt gcacgcata tcaacgtgg 
      cgttcgccc gtgtgggcc ccaatgggg tacagctga 
      ctcgggacg agctgaggg tgctccaaa ccgtggtgg 
      ggttgaagc aagtcaatg gggatttga aggtgttgg 
      tgtccgtgg gcggggctg ggaatgatg ggggctaca 
      gtggcatgg tgttaaatc acgcgagtc ggaaagttt 
      ttattcgat cggtgatcg acttagtgc ggaattata 
      gcgtggatg gggttgatc tcgattggg agtttccga 
      atgaaagtc cggggaagg accggaacc agaaggtgc 
      atttcgtgc agcttttgg aggagtttc gaatggcga 
      ttaataagc aggcgaggc ataagttta ttgtgacgg 
      ataaggggt acttttcaa cgcttaata ttaatttca 
      cggcacact attgggttt ccaaaggga tggcaaggg 
      agaaaattg tgattggtt tgcctgctt atggacaca 
      cttttcggt acatagctc aaaatcaat ggtaaaatt 
      ttactttag gcacattcg actgcataa taacacttt 
      tatcataat ttttttaaa tacaaaatt gacatttga 
      ctgtcaaat gtcaatttt ctgacatat agtagccgg 
      ctgacttga tcggatcag gtcggctct tttcgataa 
      agcaaccaa tcaaatgac ttcgaaatg accatgcaa 
      taaacctac tagataaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY