Status
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Gene information
General information
TC015559 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2308516..2310116
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015559
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09164

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015559 RA
      MLKKIYFVL LVPTILLII FYHQNKVPK KSNPDSKKS 
      LNIGNVPEI YLSDAKITK SINHSCANF PQLSDLHFN 
      NNYWQLQQT TFGDFYLFG AYLDQRHDP VVRILALYE 
      EYQPQNTYF CQLWYDNSP RPLIVESDV PYWLFRHNW 
      GADNKAYKH PYLITCPVD LIKGQKPSS VSLVEKPCD 
      NASNNLRIF ESFSKRRKN FLVCVKGLF YPFEDKTHR 
      LVEWIELLG LLGVEKIHF FELNVHPNT KKLLSYYEN 
      HGRIEVTPL SVAGSVSNN PQAVERFLR EKYTERRLQ 
      EVIPYNDCL YRHLHEFKY VVLLDTDEV IIPAENTWL 
      ELIETLQDI LPNSSSYMA RNVYFWDNP SHQHEWFEG 
      IPRHMHMLQ HVFRARKHT KPMYFVKGF HDTRLVIAL 
      HNHFPFLCY KKCDFTEID LGLAHLQHY RADCAYGVK 
      NCGVMKSES AMDTRVWNF KHELVKRVE KTLADLNLT 
      LKMEV
    CDS sequence
    • >TC015559 RA
      atgttgaaa aaaatttac ttcgtcctt ctggtaccc 
      accatcttg ttgataatt ttctaccac cagaacaag 
      gtacccaaa aaatcaaac cccgactct aaaaagtcc 
      ctaaatatc ggaaacgtc cccgaaatc tacctctcc 
      gacgcgaaa atcaccaaa tcaatcaac cacagttgt 
      gccaacttc ccccaacta tccgatctt cacttcaac 
      aacaactac tggcagtta caacaaacc acattcggg 
      gatttctac ctctttggg gcctatttg gaccaacgc 
      cacgacccc gtagttcgc atcttggcg ctgtatgag 
      gagtaccaa ccccagaac acgtacttc tgccaactg 
      tggtacgac aattccccc agaccgctc attgtcgag 
      agcgatgtt ccctattgg ttgtttcgg cataactgg 
      ggggctgat aacaaggca tacaagcac ccttatctt 
      atcacatgt ccggtggat ttgatcaaa ggacagaaa 
      ccctcaagt gtgtctttg gtcgaaaaa ccgtgtgac 
      aatgcgagc aataatttg cgaattttt gagagtttt 
      tccaaacgt cggaaaaat tttcttgtg tgtgtcaag 
      gggctgttt tacccgttt gaggacaag acgcaccgt 
      ttggtggag tggatcgaa cttttgggg ctcttgggg 
      gtggaaaaa atccacttt ttcgagcta aatgttcac 
      ccaaacacg aaaaaattg ctcagttat tacgaaaat 
      cacgggagg attgaagtc acgcctttg tcggttgct 
      gggagtgtc tcaaacaac ccccaggcg gtggagcgg 
      ttccttcgg gagaagtac acggagagg cgcctccag 
      gaggtgatc ccgtacaac gactgtctc taccgccac 
      ctccacgag ttcaaatac gtggttttg ttagatact 
      gatgaagtc attattcca gccgagaac acttggctt 
      gaattaata gaaaccttg caagatatt ctgccaaat 
      agttcgtcc tacatggcc agaaatgtg tacttttgg 
      gacaacccc tcgcatcag cacgagtgg tttgagggg 
      attccccgc cacatgcac atgttgcag cacgttttc 
      agggcgcga aagcacaca aagccgatg tatttcgtg 
      aaggggttt catgatacg agacttgtg attgcatta 
      cataatcat tttccgttc ttgtgttac aaaaagtgt 
      gattttact gagattgat ttgggtttg gcgcatttg 
      cagcattat agggctgat tgtgcctat ggggttaaa 
      aattgcggg gtgatgaag agtgagagt gctatggat 
      acgcgagtg tggaatttt aagcacgag ctagtgaag 
      cgagtggag aaaactttg gcggattta aatttgacg 
      ctaaagatg gaggtttaa
    mRNA sequence
    • >TC015559 RA
      ctacattaa aactttatc agtacttaa aaaaaacaa 
      taaatgaca ctgaaatag tgcatttcg ccgtgttat 
      taacacaat aacaagtga ttttgtaat aataccacg 
      aactcaagt aatttattt tagtatgag cacccacca 
      tgttgaaaa aaatttact tcgtccttc tggtaccca 
      ccatcttgt tgataattt tctaccacc agaacaagg 
      tacccaaaa aatcaaacc ccgactcta aaaagtccc 
      taaatatcg gaaacgtcc ccgaaatct acctctccg 
      acgcgaaaa tcaccaaat caatcaacc acagttgtg 
      ccaacttcc cccaactat ccgatcttc acttcaaca 
      acaactact ggcagttac aacaaacca cattcgggg 
      atttctacc tctttgggg cctatttgg accaacgcc 
      acgaccccg tagttcgca tcttggcgc tgtatgagg 
      agtaccaac cccagaaca cgtacttct gccaactgt 
      ggtacgaca attccccca gaccgctca ttgtcgaga 
      gcgatgttc cctattggt tgtttcggc ataactggg 
      gggctgata acaaggcat acaagcacc cttatctta 
      tcacatgtc cggtggatt tgatcaaag gacagaaac 
      cctcaagtg tgtctttgg tcgaaaaac cgtgtgaca 
      atgcgagca ataatttgc gaatttttg agagttttt 
      ccaaacgtc ggaaaaatt ttcttgtgt gtgtcaagg 
      ggctgtttt acccgtttg aggacaaga cgcaccgtt 
      tggtggagt ggatcgaac ttttggggc tcttggggg 
      tggaaaaaa tccactttt tcgagctaa atgttcacc 
      caaacacga aaaaattgc tcagttatt acgaaaatc 
      acgggagga ttgaagtca cgcctttgt cggttgctg 
      ggagtgtct caaacaacc cccaggcgg tggagcggt 
      tccttcggg agaagtaca cggagaggc gcctccagg 
      aggtgatcc cgtacaacg actgtctct accgccacc 
      tccacgagt tcaaatacg tggttttgt tagatactg 
      atgaagtca ttattccag ccgagaaca cttggcttg 
      aattaatag aaaccttgc aagatattc tgccaaata 
      gttcgtcct acatggcca gaaatgtgt acttttggg 
      acaacccct cgcatcagc acgagtggt ttgagggga 
      ttccccgcc acatgcaca tgttgcagc acgttttca 
      gggcgcgaa agcacacaa agccgatgt atttcgtga 
      aggggtttc atgatacga gacttgtga ttgcattac 
      ataatcatt ttccgttct tgtgttaca aaaagtgtg 
      attttactg agattgatt tgggtttgg cgcatttgc 
      agcattata gggctgatt gtgcctatg gggttaaaa 
      attgcgggg tgatgaaga gtgagagtg ctatggata 
      cgcgagtgt ggaatttta agcacgagc tagtgaagc 
      gagtggaga aaactttgg cggatttaa atttgacgc 
      taaagatgg aggtttaac tgtaactca atgactaat 
      aaattttgt attggatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY