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Gene information
General information
TC033366
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015550
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2191748..2193097
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033366
    iBeetle screen iB_09162

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    vitellarium size decreased - Number of animals showing the phenotype: 2

    egg chamber mostly not present - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033366 RA
      MFFTQSLLT DVTTLVVTL TVILVTYYK WSYTYWRRR 
      GVPSLTPKI PWGNLSNPL NRKDTIGVD VKKMYDSMR 
      RQNWKHGGV FFMASPVYL VTDLEYVKD ILTKNFQNF 
      VDRGLYYNE KDDPISAHL FAIGGPKWR NLRTKLTPT 
      FTSGKMKAM FQTLLDCES NLQEKVAAE LGKNQAIDI 
      KDVFGCFTT DVIGSCAFG LDCNTFKEA DSPFRKYGK 
      KVFTNTPIR QLKSSFSNS FPKLAQQFG ISVMPKDVS 
      DFFTKVVKD TVEYRQKNN YTRKDFMQL LIDLKGEGK 
      QDGKSLTMD EITAQSFLF FAAGFETSS TTVTFALYE 
      LAKHQDIQQ KVREEIETV LAKFDGKVT YEAIQEMHY 
      MNQVLNGTN YFINCFLIL IHLRTVTII KNDTTGL
    CDS sequence
    • >TC033366 RA
      atgtttttc acccaatct ctcctaacc gacgtaaca 
      actcttgtt gtaacccta actgtcatc ctagtaact 
      tactacaaa tggagttac acctactgg aggcgtcgg 
      ggagtcccc tccctgacc cccaagatc ccgtggggt 
      aacttaagc aacccttta aaccgcaag gacacaatc 
      ggagtcgac gtgaaaaaa atgtacgat tcaatgagg 
      cgccagaac tggaaacac gggggtgtt tttttcatg 
      gcgtcgcct gtttacctc gtcactgat ctcgaatat 
      gttaaggac attttgacc aaaaatttc caaaatttc 
      gtcgaccgt gggttgtat tacaacgag aaagacgat 
      ccgattagt gcccatttg ttcgccatt ggaggccca 
      aaatggcgc aatttgcga acgaaacta actcccact 
      tttacctcc gggaagatg aaagcaatg ttccagacg 
      ttgctcgac tgcgaaagc aatctccag gaaaaagtt 
      gcagcagaa ttgggaaaa aatcaagcg attgacatt 
      aaagacgtg ttcgggtgt ttcaccact gatgtcatc 
      ggctcttgt gcttttgga ctcgactgt aacactttt 
      aaagaagcc gattcgccg tttcgaaaa tacggaaaa 
      aaagtcttt acaaacact cccataaga cagctcaag 
      tcctcgttt tctaacagt tttccgaaa cttgcccaa 
      caatttgga atttctgtg atgcccaaa gatgtatca 
      gactttttc accaaagtg gtcaaggac acggtcgag 
      taccgacaa aaaaataat tacacaagg aaagacttt 
      atgcaactt ttgatcgat ttaaagggt gaaggaaaa 
      caggatggg aaaagcttg accatggat gaaattacg 
      gcacaaagt tttctcttt tttgcggcc ggttttgaa 
      acttcttcc actactgta acgttcgca ctttacgaa 
      ctcgccaaa caccaagac atccaacaa aaagttaga 
      gaggaaatt gagacagtt ttggcaaaa tttgatggg 
      aaagtgacg tatgaagcg atccaagaa atgcattac 
      atgaaccag gtcctgaac ggtacgaat tattttatt 
      aattgcttt ttgatttta attcacttg cgtacagtc 
      acgatcatt aagaatgac accactggt ttatag
    mRNA sequence
    • >TC033366 RA
      ccaactcag aatttttta aaatcattc acaaaattt 
      cagatgttt ttcacccaa tctctccta accgacgta 
      acaactctt gttgtaacc ctaactgtc atcctagta 
      acttactac aaatggagt tacacctac tggaggcgt 
      cggggagtc ccctccctg acccccaag atcccgtgg 
      ggtaactta agcaaccct ttaaaccgc aaggacaca 
      atcggagtc gacgtgaaa aaaatgtac gattcaatg 
      aggcgccag aactggaaa cacgggggt gtttttttc 
      atggcgtcg cctgtttac ctcgtcact gatctcgaa 
      tatgttaag gacattttg accaaaaat ttccaaaat 
      ttcgtcgac cgtgggttg tattacaac gagaaagac 
      gatccgatt agtgcccat ttgttcgcc attggaggc 
      ccaaaatgg cgcaatttg cgaacgaaa ctaactccc 
      acttttacc tccgggaag atgaaagca atgttccag 
      acgttgctc gactgcgaa agcaatctc caggaaaaa 
      gttgcagca gaattggga aaaaatcaa gcgattgac 
      attaaagac gtgttcggg tgtttcacc actgatgtc 
      atcggctct tgtgctttt ggactcgac tgtaacact 
      tttaaagaa gccgattcg ccgtttcga aaatacgga 
      aaaaaagtc tttacaaac actcccata agacagctc 
      aagtcctcg ttttctaac agttttccg aaacttgcc 
      caacaattt ggaatttct gtgatgccc aaagatgta 
      tcagacttt ttcaccaaa gtggtcaag gacacggtc 
      gagtaccga caaaaaaat aattacaca aggaaagac 
      tttatgcaa cttttgatc gatttaaag ggtgaagga 
      aaacaggat gggaaaagc ttgaccatg gatgaaatt 
      acggcacaa agttttctc ttttttgcg gccggtttt 
      gaaacttct tccactact gtaacgttc gcactttac 
      gaactcgcc aaacaccaa gacatccaa caaaaagtt 
      agagaggaa attgagaca gttttggca aaatttgat 
      gggaaagtg acgtatgaa gcgatccaa gaaatgcat 
      tacatgaac caggtcctg aacggtacg aattatttt 
      attaattgc tttttgatt ttaattcac ttgcgtaca 
      gtcacgatc attaagaat gacaccact ggtttatag 
      ggtcattct ttatgatcg tgactgtac atggttttt 
      tactactag ataaaacct ttccaggtt ttccaaaaa 
      tgtagaaaa aatcgcgac acattacaa ttattacaa 
      taaaaattt aagtcgatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY