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Gene information
General information
TC015540 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2072235..2076865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015540
    iBeetle screen iB_09160

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    yolk late potentiallydegenerated - Number of animals showing the phenotype: 4

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015540 RA
      MAKADSPQD LLDLCARAG CPCTAPEMA LNKLSIDAL 
      QLANKRVLM RVDFNVPLK DGKITNNQR IVAALDTIK 
      YALDKNAKS VVLMSHLGR PDGSPNPKY TLKPVAAEL 
      KTLLNRDVT FLTDCVGPD VEKACADPA PGSLILLEN 
      LRFHVEEEG KGVDASGKK IKADPAQVT AFRQSLRKL 
      GDVYVNDAF GTAHRAHSS MMGEGFEQR AAGFLLKKE 
      LQYFAKALD KPEKPFLAI LGGAKVADK IKLIENLLD 
      KVQEMIIGG GMSYTFLKV TKDMKIGGS LYDDEGAKI 
      VNNLLAKAK QNNVQIHLP CDFVTADKF DEKAQVGAA 
      TLEAGIPDG WMGLDIGPK SIEAFKAPI QRAKVIVWN 
      GPAGVFEFE NFAKGTKAI MDEVVAATQ RGCVTIIGG 
      GDTATCAAK WKTEDKVSH VSTGGGASL ELLEGKVLP 
      GVAALSDA
    CDS sequence
    • >TC015540 RA
      atggcaaaa gcagattcc ccccaagat ttgctcgat 
      ttgtgtgcc cgtgccggt tgtccctgt accgccccc 
      gaaatggct ttaaacaaa ctatcaatt gatgcttta 
      caactcgcc aacaaacgc gtccttatg agggttgac 
      ttcaacgtc cccttaaag gacggcaag atcaccaac 
      aaccagcgc atcgtcgcc gccctcgac accatcaaa 
      tacgccctt gacaagaac gccaaaagc gtcgtcctc 
      atgtcgcac ttgggccgc cctgacggc agccccaac 
      cccaaatac accctcaag ccggtggcc gccgagctc 
      aaaaccctc ctcaacaga gacgtcacg ttcctcacc 
      gactgcgtc ggccccgac gtcgagaag gcgtgcgct 
      gaccccgcc cccggctca ctcatccta ttggaaaac 
      ttgcgtttc cacgtcgag gaggagggc aaaggggtg 
      gacgcctct ggcaagaaa atcaaagct gaccccgcc 
      caagtcacc gccttccgg caaagtctg cgcaaattg 
      ggcgacgtt tacgtcaat gacgccttc gggaccgcc 
      caccgcgcc cactcctcc atgatgggg gaggggttt 
      gagcagcgc gccgccggc ttcctcctc aaaaaggag 
      ttacaatat ttcgcaaag gcgttggac aagcccgaa 
      aaaccgttc cttgcgatt ttgggcggg gctaaagtt 
      gccgataaa atcaaactc atcgaaaat ctactggat 
      aaggtccag gaaatgatc atagggggc gggatgagt 
      tacaccttc cttaaagta acgaaagac atgaagatt 
      gggggttcg ttgtacgac gacgagggg gcgaagatc 
      gtcaataat ttgctcgcg aaagcgaag caaaacaac 
      gtgcaaatt cacttacct tgcgatttc gtcacggcg 
      gacaaattc gacgagaag gcgcaagtg ggggctgcc 
      actctcgag gcggggatc cccgacggc tggatgggg 
      ctggacatc gggccgaag tcgatcgaa gcgttcaaa 
      gcccccatt cagagggcc aaagtgata gtgtggaac 
      gggccagcc ggtgttttc gagtttgaa aattttgca 
      aaagggacc aaagccata atggatgaa gtcgtggcg 
      gcgactcaa aggggctgt gtcacgatt attggtggc 
      ggtgacacg gccacttgt gccgcaaaa tggaagact 
      gaggataag gtttcgcac gtttcgacg ggcgggggc 
      gccagtttg gagctactg gagggcaag gtcctacca 
      ggggtggcc gccctctcc gacgcttga
    mRNA sequence
    • >TC015540 RA
      tacttacaa tgggcaaag ataattatt aaatgaaaa 
      atttttgac ttgtttaga cacaatgtt tgtttaata 
      atcagattt agcaacttt ttttgatta aaataatgc 
      ggaaaaaca caattttgc aaaattggg caaacaaaa 
      gtgacctct atctgacat tgaatggca aaagcagat 
      tccccccaa gatttgctc gatttgtgt gcccgtgcc 
      ggttgtccc tgtaccgcc cccgaaatg gctttaaac 
      aaactatca attgatgct ttacaactc gccaacaaa 
      cgcgtcctt atgagggtt gacttcaac gtcccctta 
      aaggacggc aagatcacc aacaaccag cgcatcgtc 
      gccgccctc gacaccatc aaatacgcc cttgacaag 
      aacgccaaa agcgtcgtc ctcatgtcg cacttgggc 
      cgccctgac ggcagcccc aaccccaaa tacaccctc 
      aagccggtg gccgccgag ctcaaaacc ctcctcaac 
      agagacgtc acgttcctc accgactgc gtcggcccc 
      gacgtcgag aaggcgtgc gctgacccc gcccccggc 
      tcactcatc ctattggaa aacttgcgt ttccacgtc 
      gaggaggag ggcaaaggg gtggacgcc tctggcaag 
      aaaatcaaa gctgacccc gcccaagtc accgccttc 
      cggcaaagt ctgcgcaaa ttgggcgac gtttacgtc 
      aatgacgcc ttcgggacc gcccaccgc gcccactcc 
      tccatgatg ggggagggg tttgagcag cgcgccgcc 
      ggcttcctc ctcaaaaag gagttacaa tatttcgca 
      aaggcgttg gacaagccc gaaaaaccg ttccttgcg 
      attttgggc ggggctaaa gttgccgat aaaatcaaa 
      ctcatcgaa aatctactg gataaggtc caggaaatg 
      atcataggg ggcgggatg agttacacc ttccttaaa 
      gtaacgaaa gacatgaag attgggggt tcgttgtac 
      gacgacgag ggggcgaag atcgtcaat aatttgctc 
      gcgaaagcg aagcaaaac aacgtgcaa attcactta 
      ccttgcgat ttcgtcacg gcggacaaa ttcgacgag 
      aaggcgcaa gtgggggct gccactctc gaggcgggg 
      atccccgac ggctggatg gggctggac atcgggccg 
      aagtcgatc gaagcgttc aaagccccc attcagagg 
      gccaaagtg atagtgtgg aacgggcca gccggtgtt 
      ttcgagttt gaaaatttt gcaaaaggg accaaagcc 
      ataatggat gaagtcgtg gcggcgact caaaggggc 
      tgtgtcacg attattggt ggcggtgac acggccact 
      tgtgccgca aaatggaag actgaggat aaggtttcg 
      cacgtttcg acgggcggg ggcgccagt ttggagcta 
      ctggagggc aaggtccta ccaggggtg gccgccctc 
      tccgacgct tgattttcc cgaactttg ttgtaaatt 
      attgtaata aactagtga ttttgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY