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Gene information
General information
TC015534 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2030881..2032703
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015534
    iBeetle screen iB_09159

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially dorsalnot fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015534 RA
      MWPEVQRAR SENRHELVL GGNEIAERI AKEGLDPGI 
      FALTGLNYL DLHETSLGA IPDEIARLV NLQSLVLHS 
      NKLEGVNSA VTKLEKLKL LDLARNQLR EVPPEIDKL 
      ANIVTFNFT FNCLGGFPE LRNTRKLSV LDLSNNKLK 
      IFPRVCNEG LANLSELKL SENEIETIP PEINQLTGL 
      KVLELGHNK IKSLPGELA DCTKLKVLG LKNNPISDR 
      RLLKLIDQC RTKQIIDYV KAHSPKTVV QKSEQKGPP 
      RATQDSDSD PDEYKHTIR VHYAKDSPK IVLDESVKS 
      VREFLVACL VSVTFTEET FKKFIQIQN KLHETVCSK 
      RNSSTIATH DYDKLPPGD LHYTTLPPS ELQIQPLNR 
      PTAMSGADL FTKLQTEAN NLRKEKKRN TYSGIHKFL 
      YLIEGKSRY PCLVNSQGI VVSFPPITN SEVTKIEVG 
      TKNLLVEVT SSVSLHLCK VAMEALLRE LIGLVGHDL 
      EVTQVKSTD PDGNLRVVY PSKNDLVFE GNEIRVVRD
    CDS sequence
    • >TC015534 RA
      atgtggccc gaggtccag agggctagg agtgagaac 
      cggcacgag ttggtgctc gggggcaac gagattgcc 
      gaacgcatt gccaaggag gggctagat ccggggatt 
      tttgccctg acagggctg aactacctg gatttgcac 
      gagaccagt ctgggggct attccggac gaaatcgcc 
      cgattggtg aacttgcag tcgctggtg cttcactcg 
      aacaagctc gagggggtt aacagtgcg gtcaccaag 
      ctggagaag ttgaagctt ttggatttg gcacgaaac 
      caactgagg gaagtccct cctgagatt gacaagttg 
      gccaatatt gtgaccttt aatttcact tttaactgt 
      cttgggggt ttccccgag ttgcgcaac acgcggaaa 
      ttgtcagtc ttggacttg tcgaacaat aaattgaaa 
      atttttccg cgggtttgt aatgaaggg ttggctaat 
      ttgtccgaa ctgaagctc agtgagaat gaaattgag 
      acaatccct ccggaaatt aaccaatta acgggcctg 
      aaggtgcta gagttgggg cacaataaa atcaagtcg 
      cttccgggc gaactagcc gattgtaca aaacttaaa 
      gttttgggt ttgaaaaac aacccaatt tccgaccgt 
      cgcctcctt aaactaatc gaccaatgt cgcactaaa 
      caaatcatc gactacgtc aaggcccac agccccaaa 
      actgtggtg caaaagagc gaacaaaag ggcccccct 
      agggccacc caagactcc gacagtgac cccgacgag 
      tacaaacac accattcgg gtccactat gccaaagac 
      agccccaaa atagtgcta gacgagagc gtgaaaagc 
      gtgcgagaa ttcctagtc gcttgtctc gtctctgtc 
      actttcacc gaagaaacg ttcaagaaa tttatccaa 
      atccagaat aaattacac gagaccgtg tgttctaaa 
      cgcaactcg tccacaatc gccacccac gactacgac 
      aaattgccc cccggggat ttacactac acgactttg 
      cccccctcc gagctccaa atccagccc ctcaaccgc 
      cccacggcc atgtcgggg gccgacctc ttcaccaag 
      ctccaaacc gaggctaat aatctacgc aaagagaag 
      aaacgtaac acttattcg gggattcac aagttcttg 
      tatctaatc gaggggaag tcccgatac ccctgtctg 
      gtcaattcg caagggatt gtcgtctca ttcccgcca 
      atcactaac tcggaagtg accaaaatt gaagtgggg 
      acgaaaaat ttgctggtt gaagtgaca agttccgtg 
      tctttgcat ttgtgtaaa gttgcaatg gaggcactt 
      ttgagggag ttgatcggt ctggtgggg catgacttg 
      gaggtcacg caagtcaaa agcacggat ccggacggg 
      aatttacgg gtggtttac ccgtccaag aatgatctg 
      gtgtttgaa gggaacgaa attagagtt gttagggac 
      taa
    mRNA sequence
    • >TC015534 RA
      agttagggg aatttcgat ttggtgccg tttcccgcc 
      gggtttttc tcggaaatt tcggcgaaa attgcgcaa 
      ttttgccca actgggcct tttagggtc gtgtcccga 
      gcgtttggt gccaattgg gtgagtttc gtgaaccta 
      accttgaaa tgtggcccg aggtccaga gggctagga 
      gtgagaacc ggcacgagt tggtgctcg ggggcaacg 
      agattgccg aacgcattg ccaaggagg ggctagatc 
      cggggattt ttgccctga cagggctga actacctgg 
      atttgcacg agaccagtc tgggggcta ttccggacg 
      aaatcgccc gattggtga acttgcagt cgctggtgc 
      ttcactcga acaagctcg agggggtta acagtgcgg 
      tcaccaagc tggagaagt tgaagcttt tggatttgg 
      cacgaaacc aactgaggg aagtccctc ctgagattg 
      acaagttgg ccaatattg tgaccttta atttcactt 
      ttaactgtc ttgggggtt tccccgagt tgcgcaaca 
      cgcggaaat tgtcagtct tggacttgt cgaacaata 
      aattgaaaa tttttccgc gggtttgta atgaagggt 
      tggctaatt tgtccgaac tgaagctca gtgagaatg 
      aaattgaga caatccctc cggaaatta accaattaa 
      cgggcctga aggtgctag agttggggc acaataaaa 
      tcaagtcgc ttccgggcg aactagccg attgtacaa 
      aacttaaag ttttgggtt tgaaaaaca acccaattt 
      ccgaccgtc gcctcctta aactaatcg accaatgtc 
      gcactaaac aaatcatcg actacgtca aggcccaca 
      gccccaaaa ctgtggtgc aaaagagcg aacaaaagg 
      gccccccta gggccaccc aagactccg acagtgacc 
      ccgacgagt acaaacaca ccattcggg tccactatg 
      ccaaagaca gccccaaaa tagtgctag acgagagcg 
      tgaaaagcg tgcgagaat tcctagtcg cttgtctcg 
      tctctgtca ctttcaccg aagaaacgt tcaagaaat 
      ttatccaaa tccagaata aattacacg agaccgtgt 
      gttctaaac gcaactcgt ccacaatcg ccacccacg 
      actacgaca aattgcccc ccggggatt tacactaca 
      cgactttgc ccccctccg agctccaaa tccagcccc 
      tcaaccgcc ccacggcca tgtcggggg ccgacctct 
      tcaccaagc tccaaaccg aggctaata atctacgca 
      aagagaaga aacgtaaca cttattcgg ggattcaca 
      agttcttgt atctaatcg aggggaagt cccgatacc 
      cctgtctgg tcaattcgc aagggattg tcgtctcat 
      tcccgccaa tcactaact cggaagtga ccaaaattg 
      aagtgggga cgaaaaatt tgctggttg aagtgacaa 
      gttccgtgt ctttgcatt tgtgtaaag ttgcaatgg 
      aggcacttt tgagggagt tgatcggtc tggtggggc 
      atgacttgg aggtcacgc aagtcaaaa gcacggatc 
      cggacggga atttacggg tggtttacc cgtccaaga 
      atgatctgg tgtttgaag ggaacgaaa ttagagttg 
      ttagggact aaactaatt tatttattc gaatataag 
      ttttttgta tttctggta tttaataaa gaacgaggt 
      tcatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY