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Gene information
General information
TC015215 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1354940..1360540
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015215
    iBeetle screen iB_09155

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015215 RA
      MSFFGFGQS AEIEIYLDG QETRKTAEV KTEDGKKER 
      LLLYYDGET VSGKVNISL KKPGSKLEH QGIKIEFIG 
      QIEMFYDRG NHHEFISLV KELARPGDM IAHTSYPFE 
      FTCVEKPFE VYTGANVRL RYFLKVTII RRLADIVKE 
      LDIAVHTLS SYPEMNNSI KMEVGIEDC LHIEFEYNK 
      SKYHLKDVI VGKIYFLLV RIKIKHMEI AIIKKETTG 
      TGPNTFTEN ETIAKYEIM DGAPVRGES IPIRVFLAG 
      YELTPTMRD INKKFSVRY FLNLVLMDT EDRRYFKQQ 
      EITLWRKGD KVRKPNLQS PAILAGTQN LTNTAQQIS 
      SQEENQEPA QKEDFLRAP NSQSDEKLN SNDPFDQGI 
      NSPTESPPD SHIQNMTRE AGDGQNDPE NTE
    CDS sequence
    • >TC015215 RA
      atgagtttc ttcggtttc ggacaatcc gccgagatc 
      gagatctat ttagatgga caagagacc agaaaaact 
      gccgaagtc aagactgaa gatgggaaa aaagagcga 
      cttttgctc tactacgac ggcgaaacg gtgtctggc 
      aaggtgaac ataagttta aaaaaaccg ggcagcaag 
      ctggagcac cagggtatc aaaatcgag tttattggc 
      caaattgag atgttctac gaccgaggc aaccaccat 
      gaatttatc tcgttagtg aaagagttg gcgagacca 
      ggcgacatg atcgcacac accagctac ccttttgaa 
      ttcacttgc gtggagaag cccttcgaa gtgtacacg 
      ggggccaac gtcagactg agatacttc ctaaaagtg 
      acgataatt cgacgactg gccgacatc gtcaaagag 
      ctagacatt gccgtacac acgctatca agttacccc 
      gaaatgaac aactcgatt aaaatggaa gtggggatt 
      gaggactgt cttcacatc gagtttgaa tacaacaag 
      tccaaatat catctcaag gacgtgatt gttggcaag 
      atttatttc ctacttgtt cgtatcaag ataaagcat 
      atggaaatt gcgattatt aagaaggag accactgga 
      actgggcct aacacgttc actgaaaat gaaactatt 
      gcgaagtat gaaattatg gatggggct ccagtcagg 
      ggtgaaagt attccgatt agggttttt ttggctggg 
      tatgaactt acacccact atgcgagat attaataag 
      aaattttcg gtgaggtat ttcctcaat ttggttcta 
      atggatacg gaagatcgg cgatatttc aagcaacag 
      gaaatcaca ctctggcgg aaaggagac aaagtacga 
      aaaccgaac ctccaaagc cccgcaatc ctcgccggc 
      acccaaaac ctgacaaac acagctcag caaatcagc 
      agtcaggag gagaatcaa gaaccggca caaaaagaa 
      gatttttta cgcgctcca aattcccaa tcagatgaa 
      aaattgaat tcaaacgat ccattcgat caaggaatc 
      aattcgccg actgaaagc cccccagat agtcacata 
      cagaacatg actcgggag gctggcgat ggacaaaat 
      gatcccgaa aacaccgag tga
    mRNA sequence
    • >TC015215 RA
      gtaacggcg gccatcttg caggtgccc tttggtaaa 
      agtccctct cttattata aattttttt aaatcacca 
      ccccaaaca aaaatgatt ttaaactaa gctcggctg 
      tgttgtgtt ggaatcaaa gactcgaat aaatcagtt 
      ttttttaaa gcaatcgtg tacagcagt ttgttagaa 
      tgagtttct tcggtttcg gacaatccg ccgagatcg 
      agatctatt tagatggac aagagacca gaaaaactg 
      ccgaagtca agactgaag atgggaaaa aagagcgac 
      ttttgctct actacgacg gcgaaacgg tgtctggca 
      aggtgaaca taagtttaa aaaaaccgg gcagcaagc 
      tggagcacc agggtatca aaatcgagt ttattggcc 
      aaattgaga tgttctacg accgaggca accaccatg 
      aatttatct cgttagtga aagagttgg cgagaccag 
      gcgacatga tcgcacaca ccagctacc cttttgaat 
      tcacttgcg tggagaagc ccttcgaag tgtacacgg 
      gggccaacg tcagactga gatacttcc taaaagtga 
      cgataattc gacgactgg ccgacatcg tcaaagagc 
      tagacattg ccgtacaca cgctatcaa gttaccccg 
      aaatgaaca actcgatta aaatggaag tggggattg 
      aggactgtc ttcacatcg agtttgaat acaacaagt 
      ccaaatatc atctcaagg acgtgattg ttggcaaga 
      tttatttcc tacttgttc gtatcaaga taaagcata 
      tggaaattg cgattatta agaaggaga ccactggaa 
      ctgggccta acacgttca ctgaaaatg aaactattg 
      cgaagtatg aaattatgg atggggctc cagtcaggg 
      gtgaaagta ttccgatta gggtttttt tggctgggt 
      atgaactta cacccacta tgcgagata ttaataaga 
      aattttcgg tgaggtatt tcctcaatt tggttctaa 
      tggatacgg aagatcggc gatatttca agcaacagg 
      aaatcacac tctggcgga aaggagaca aagtacgaa 
      aaccgaacc tccaaagcc ccgcaatcc tcgccggca 
      cccaaaacc tgacaaaca cagctcagc aaatcagca 
      gtcaggagg agaatcaag aaccggcac aaaaagaag 
      attttttac gcgctccaa attcccaat cagatgaaa 
      aattgaatt caaacgatc cattcgatc aaggaatca 
      attcgccga ctgaaagcc ccccagata gtcacatac 
      agaacatga ctcgggagg ctggcgatg gacaaaatg 
      atcccgaaa acaccgagt gatacaggg tggttattt 
      atacgaatc ggacttgaa cgagttgga aatgtgatc 
      tttgtttgg ggcgtaaat ggcaggcgt gtgcacgtt 
      tgtaaatat tgtaattgt tattactac agttggcat 
      tctgtataa ataagcaaa tttattatt tacacataa 
      atattattg tatatttct catatagtg aataaacaa 
      gttacgaca tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY