Status
Please wait ...
Gene information
General information
TC015481 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9340859..9354113
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015481
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09154

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva dorsal bent - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs podomer number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax not fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015481 RA
      MIPRPRCKL RTVLIVTLL VVLLVHISS SERVTRRRL 
      RRPLKTTQS VSVTKDQDV SSSVNRFRL RSRPGHRKT 
      DNSLSATSK KDKDGYKVV CYYTNWSQY RVKIGKFTP 
      EDIIPDLCT HIIFAFGWL KKGKLSSFE SNDETKDGK 
      VGLYERIQK LKKANPSLK TLLAIGGWS FGTQKFKDM 
      ASTRYARQT FIFSAIPFL RDRGFDGLD IDWEYPKGS 
      EDKKNYVLL LKELREAFE AEAQEIKKP ALLLSAAVP 
      VGPDNIKAG YDVPAVSSY LDFINLMAY DFHGKWERE 
      TGHNAPLYS PSSDSQYQK QLNVEHAAN LWVKLGTPK 
      EKLVIGMPT YGRTFTLSN TAKFGVHAP ASGGGKEGT 
      YTKESGFLA YYEVCELLQ NGATYIWDD EMKVPYAVN 
      GDQWIGFDD EKSIRNKMK WIKDNGFAG AMVWTIDMD 
      DFSGTVCGG QVKYPLIGA MREELRGIS RGKNAKDVD 
      WQKIAGAVE EDDEDAIEK PAPVKISVQ EVLNRVRKP 
      TKKLHLKNS PISKKVRPP QVLCYITSW SQKRPGAGK 
      FTPEDINPS LCTHIIYAF ATLKDHKLA EASDKDPEM 
      YDRVVALRE KNPDLKILL AIGGWAFGS TPFKELTSN 
      VFRMNQFVY DAIDFLREF QFNGLDVDW EYPRGADDR 
      AAYVNLLKE LRVAFEGEA KTSGQPRLL LTAAVPASF 
      EAIAAGYDV PEISKYLDF INVMTYDFH GQWERTVGH 
      NSPLFPLES ATSYQKKLT VDFSAREWV KQGAPKEKL 
      MIGMPTYGR SFELVNKTQ FDIGAPASG GGTPGKYTA 
      EAGFMSFYE ICDFLHEDN TTLVWDNEQ QVPFAYRGN 
      QWVGFDDER SLKTKMAWL KEEGFGGIM IWSVDMDDF 
      RGSCGSGKY PLINAMRQE LEGYKVKLE YDGPYETSI 
      SSGQYTTKD PNEVTCDEE EGHISYHPD KADCRMYYM 
      CEGERKHHM PCPSNLVFN PDQNVCDWP ENVEGCMQH 
      TAAPPTKR
    CDS sequence
    • >TC015481 RA
      atgataccg cgaccccgt tgcaaatta cgaaccgtc 
      ctcatcgtc accctctta gtcgtcctc ctagtccac 
      atctcctca tcagaacga gtcacacgc cgccgcctt 
      cgacggccc ctcaaaacc acacaaagc gtttcagta 
      accaaagac caagacgtc tcctccagt gtcaaccgt 
      ttccggctg aggtcccgg ccaggccac cgcaaaaca 
      gacaattcc ctctcagcc acgagcaag aaggacaaa 
      gatgggtac aaagtggtg tgctattac actaactgg 
      tcgcagtac agagtcaag atcgggaaa ttcaccccg 
      gaggacatc atcccggat ttgtgcacg cacatcatc 
      ttcgccttc ggctggctg aagaaaggc aaactgtct 
      tcgttcgag agcaacgac gagaccaaa gacgggaaa 
      gtggggttg tacgaaagg atccagaag ctgaagaag 
      gccaacccc agtttgaaa accttgttg gctatcggg 
      ggttggtcg ttcggaacg cagaaattc aaggacatg 
      gcgtccacc aggtacgcc agacagacg ttcatcttc 
      agcgccatc cccttcctg cgggaccgc ggattcgac 
      gggctggac atcgactgg gagtacccc aaggggtcg 
      gaggacaag aagaattac gtcctgctg ttgaaagaa 
      ttgcgcgaa gccttcgaa gccgaagcc caggaaatc 
      aaaaagccg gctctcctt ctctcggct gccgtccct 
      gtaggtccc gataacatc aaagctggg tatgacgtc 
      ccggctgtg tcttcctac ctggacttc atcaacttg 
      atggcttac gatttccat ggtaaatgg gaacgggag 
      acggggcat aatgccccc ctctactcc ccgagcagc 
      gatagccag taccagaag caactaaac gtggaacat 
      gccgcaaac ctttgggtg aaactaggg actcctaag 
      gagaaactg gtcattggg atgcccacc tacgggcgc 
      acgttcacc ctttcgaac actgccaag ttcggggtg 
      cacgcccca gcgagtggc ggcggcaaa gaaggcacc 
      tatacgaag gagtccggg ttcttggcc tattacgaa 
      gtgtgcgag ttgcttcag aacggggca acgtacatt 
      tgggacgac gagatgaag gtgccttac gcggtcaat 
      ggagaccag tggattggg tttgatgac gaaaaatcg 
      atcaggaac aaaatgaag tggatcaag gataatggg 
      tttgcgggc gctatggtg tggaccatc gacatggac 
      gacttctcc gggacggtc tgcgggggc caggtcaag 
      taccccctg atcggggcc atgcgggag gagctgcgc 
      gggatctcc cgcgggaag aacgccaaa gacgtcgac 
      tggcaaaaa atcgccggc gctgtggag gaagacgac 
      gaagacgcg atcgagaaa ccggccccg gtcaagatt 
      tcggtccag gaagtgctc aacagagtt aggaaaccg 
      accaagaaa ctccatttg aaaaattca ccaatcagc 
      aagaaagtc cgtccgcca caagtcctc tgctacatc 
      accagttgg tcgcagaag cgccccggc gctggcaaa 
      ttcacacca gaggacata aatccgtcc ctttgcact 
      cacattatt tacgctttc gccactctc aaagaccac 
      aaactggcc gaagccagc gataaagac ccggaaatg 
      tacgaccgg gttgtggcc ctccgggag aaaaacccc 
      gatttgaag atactcttg gcaatcgga ggatgggcg 
      ttcggctca actccgttc aaagaactc acaagtaac 
      gtcttcaga atgaaccaa ttcgtctac gacgctatt 
      gacttcttg cgcgagttc caattcaac ggtttggac 
      gtcgactgg gagtaccct cgcggggct gacgaccga 
      gctgcgtac gtcaatctc ttgaaagag ttgagagtg 
      gcgttcgaa ggcgaggcg aagacgagt ggacaacct 
      cgccttctc ctaacagca gcggtacca gcgtccttt 
      gaagcaatt gccgccggg tatgacgta cccgaaatt 
      tccaagtac ctcgacttt atcaacgtc atgacgtac 
      gattttcat ggccaatgg gaacgaact gtaggacac 
      aactcacct ttgttcccg cttgaaagt gccacaagt 
      taccagaaa aagctcaca gtggacttc tctgcccgg 
      gaatgggtc aaacaaggg gcccctaaa gaaaaactc 
      atgatcggt atgcccacc tacggccgt agcttcgaa 
      ctagtgaac aagacccag ttcgatatt ggggcccca 
      gccagtggc ggtggtact cctgggaaa tacaccgcc 
      gaggccggt tttatgagc ttctacgaa atttgcgac 
      tttttgcac gaagacaac acaaccctg gtatgggac 
      aacgaacag caggtcccg ttcgcgtac cgaggcaac 
      caatgggtg ggattcgac gacgaaagg agtttgaaa 
      acgaagatg gcctggctg aaggaggag ggctttggg 
      ggcataatg atctggtcg gtggacatg gacgacttc 
      cgcggctcc tgcggcagc ggcaagtac cccctgatc 
      aatgccatg cggcaggag ctcgaaggc tacaaagtg 
      aaactggaa tacgacggc ccttacgaa acgtcgatc 
      tcctcagga cagtacacg acgaaagat ccaaatgag 
      gtgacttgc gatgaggag gaaggtcat atcagttat 
      caccccgat aaggccgat tgtcggatg tattacatg 
      tgtgaaggt gagaggaag caccacatg ccttgtcct 
      tcgaatcta gtgttcaat ccggaccag aacgtctgt 
      gactggccc gagaacgtc gaagggtgc atgcaacac 
      acggctgcg cctcccact aagcgataa
    mRNA sequence
    • >TC015481 RA
      gtgtcaaag agagacacc gattcgtaa ctttcgaat 
      tggtgtgct atatgtttg tggtggaga gagacggct 
      cgagacagt tctgggggg ctggagaga gacaagtgc 
      ggctggtag gggccttat cgagcgaaa ttcatacac 
      tctttgaat tcagagtgg cagttaagc tgaagacgt 
      cgatttcga attgtagcg cgcattgtg aagagttac 
      atgagtgcc agtggagtg ccccgtttg tacagtgac 
      agtgatttt gaactggtg tgaagtttt ttcaagtga 
      aaaatgata ccgcgaccc cgttgcaaa ttacgaacc 
      gtcctcatc gtcaccctc ttagtcgtc ctcctagtc 
      cacatctcc tcatcagaa cgagtcaca cgccgccgc 
      cttcgacgg cccctcaaa accacacaa agcgtttca 
      gtaaccaaa gaccaagac gtctcctcc agtgtcaac 
      cgtttccgg ctgaggtcc cggccaggc caccgcaaa 
      acagacaat tccctctca gccacgagc aagaaggac 
      aaagatggg tacaaagtg gtgtgctat tacactaac 
      tggtcgcag tacagagtc aagatcggg aaattcacc 
      ccggaggac atcatcccg gatttgtgc acgcacatc 
      atcttcgcc ttcggctgg ctgaagaaa ggcaaactg 
      tcttcgttc gagagcaac gacgagacc aaagacggg 
      aaagtgggg ttgtacgaa aggatccag aagctgaag 
      aaggccaac cccagtttg aaaaccttg ttggctatc 
      gggggttgg tcgttcgga acgcagaaa ttcaaggac 
      atggcgtcc accaggtac gccagacag acgttcatc 
      ttcagcgcc atccccttc ctgcgggac cgcggattc 
      gacgggctg gacatcgac tgggagtac cccaagggg 
      tcggaggac aagaagaat tacgtcctg ctgttgaaa 
      gaattgcgc gaagccttc gaagccgaa gcccaggaa 
      atcaaaaag ccggctctc cttctctcg gctgccgtc 
      cctgtaggt cccgataac atcaaagct gggtatgac 
      gtcccggct gtgtcttcc tacctggac ttcatcaac 
      ttgatggct tacgatttc catggtaaa tgggaacgg 
      gagacgggg cataatgcc cccctctac tccccgagc 
      agcgatagc cagtaccag aagcaacta aacgtggaa 
      catgccgca aacctttgg gtgaaacta gggactcct 
      aaggagaaa ctggtcatt gggatgccc acctacggg 
      cgcacgttc accctttcg aacactgcc aagttcggg 
      gtgcacgcc ccagcgagt ggcggcggc aaagaaggc 
      acctatacg aaggagtcc gggttcttg gcctattac 
      gaagtgtgc gagttgctt cagaacggg gcaacgtac 
      atttgggac gacgagatg aaggtgcct tacgcggtc 
      aatggagac cagtggatt gggtttgat gacgaaaaa 
      tcgatcagg aacaaaatg aagtggatc aaggataat 
      gggtttgcg ggcgctatg gtgtggacc atcgacatg 
      gacgacttc tccgggacg gtctgcggg ggccaggtc 
      aagtacccc ctgatcggg gccatgcgg gaggagctg 
      cgcgggatc tcccgcggg aagaacgcc aaagacgtc 
      gactggcaa aaaatcgcc ggcgctgtg gaggaagac 
      gacgaagac gcgatcgag aaaccggcc ccggtcaag 
      atttcggtc caggaagtg ctcaacaga gttaggaaa 
      ccgaccaag aaactccat ttgaaaaat tcaccaatc 
      agcaagaaa gtccgtccg ccacaagtc ctctgctac 
      atcaccagt tggtcgcag aagcgcccc ggcgctggc 
      aaattcaca ccagaggac ataaatccg tccctttgc 
      actcacatt atttacgct ttcgccact ctcaaagac 
      cacaaactg gccgaagcc agcgataaa gacccggaa 
      atgtacgac cgggttgtg gccctccgg gagaaaaac 
      cccgatttg aagatactc ttggcaatc ggaggatgg 
      gcgttcggc tcaactccg ttcaaagaa ctcacaagt 
      aacgtcttc agaatgaac caattcgtc tacgacgct 
      attgacttc ttgcgcgag ttccaattc aacggtttg 
      gacgtcgac tgggagtac cctcgcggg gctgacgac 
      cgagctgcg tacgtcaat ctcttgaaa gagttgaga 
      gtggcgttc gaaggcgag gcgaagacg agtggacaa 
      cctcgcctt ctcctaaca gcagcggta ccagcgtcc 
      tttgaagca attgccgcc gggtatgac gtacccgaa 
      atttccaag tacctcgac tttatcaac gtcatgacg 
      tacgatttt catggccaa tgggaacga actgtagga 
      cacaactca cctttgttc ccgcttgaa agtgccaca 
      agttaccag aaaaagctc acagtggac ttctctgcc 
      cgggaatgg gtcaaacaa ggggcccct aaagaaaaa 
      ctcatgatc ggtatgccc acctacggc cgtagcttc 
      gaactagtg aacaagacc cagttcgat attggggcc 
      ccagccagt ggcggtggt actcctggg aaatacacc 
      gccgaggcc ggttttatg agcttctac gaaatttgc 
      gactttttg cacgaagac aacacaacc ctggtatgg 
      gacaacgaa cagcaggtc ccgttcgcg taccgaggc 
      aaccaatgg gtgggattc gacgacgaa aggagtttg 
      aaaacgaag atggcctgg ctgaaggag gagggcttt 
      gggggcata atgatctgg tcggtggac atggacgac 
      ttccgcggc tcctgcggc agcggcaag taccccctg 
      atcaatgcc atgcggcag gagctcgaa ggctacaaa 
      gtgaaactg gaatacgac ggcccttac gaaacgtcg 
      atctcctca ggacagtac acgacgaaa gatccaaat 
      gaggtgact tgcgatgag gaggaaggt catatcagt 
      tatcacccc gataaggcc gattgtcgg atgtattac 
      atgtgtgaa ggtgagagg aagcaccac atgccttgt 
      ccttcgaat ctagtgttc aatccggac cagaacgtc 
      tgtgactgg cccgagaac gtcgaaggg tgcatgcaa 
      cacacggct gcgcctccc actaagcga taatacgta 
      acttaaact ttgtaaata attggaaat ttagtcttg 
      tttagtaac acggaattt gtttgtttt aataattta 
      agtgattat gttactgcc attgactga aaaagattc 
      ttttgtgaa actgccata aggaaagac agttttttg 
      cactcgtat tgggggatc aattttaat aaattgttt 
      tatcgagtt atttaactt tctatttta tataataat 
      ttataaatt atgcattgt agatgtagc gaaagaata 
      tttttatat ggtatacat tttgtatat tgtaacact 
      tgtaaatac acataaata aattttaaa aactggc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY