Status
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Gene information
General information
TC033349
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015231,TC015232
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9355065..9366545
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033349
    iBeetle screen iB_09153

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    legs sometimes size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs femur sometimes potentiallyfused - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs tibiotarsus sometimes potentiallyfused - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033349 RA
      MRLLYFNTF VLYSFFGYV YNEEKSLQY SIYDEETNM 
      VITTINSTV VEIEEDTTL VNVDLQENT KLCNKMFNL 
      SSKVESLII TGPHVVEIE TNFLHSQNV YELSISNTG 
      ITTIKKYTF KNLKIQTLE LFDNEIQTL EAKAFADLF 
      QLHKLDLSN NDLTTLDLE SFYRLPLLD YFDAKGNQI 
      VKLGKQFFQ ILRKDYSEM WLCSNKIQV LGVEFFEGL 
      GVNNISIDL NRNLIEFIS PHVFQGHSF REIDLSFNP 
      VTISEDFFR SELEIKTFV FNLDYLHHG TVEKLRYIA 
      GSFRPLVRC CPLAACPPA AVRRLRERR RSQPRPMDA 
      STLSAMDKD ALKKQIENM KYQASMERW PLSKSIAAM 
      REYVEENEK NDPLIHAPD KKNNPWAEK GKCVIM
    CDS sequence
    • >TC033349 RA
      atgcgactt ttgtatttt aacacgttt gttctctat 
      tctttcttc ggttatgtg tataatgaa gaaaaatca 
      ctccagtat tcaatttac gacgaagaa accaacatg 
      gtcataaca acaattaac tcaactgtc gtggaaatc 
      gaagaagac actacttta gtaaatgtc gacttgcaa 
      gagaacaca aaattgtgc aacaaaatg ttcaatctg 
      tcttcgaaa gtcgagtct ttaattatt acaggcccc 
      catgttgtg gaaattgag acaaatttt ttacactcc 
      cagaacgta tacgagctc tctatttca aacactgga 
      ataactacg atcaaaaaa tacactttc aaaaatcta 
      aaaatacaa actttagaa ttgtttgat aacgaaatt 
      caaacgctt gaagctaaa gcatttgcc gacttattc 
      cagttacat aaactggac ttgtccaac aacgattta 
      acgacgttg gacctagaa agtttctac agactgccc 
      cttcttgat tactttgat gctaaaggg aatcaaatc 
      gtgaaattg ggaaaacag tttttccaa attttgcgc 
      aaggattat tccgaaatg tggctttgt agcaacaaa 
      atccaagtt ctaggtgtg gagtttttc gaaggtttg 
      ggtgtaaat aacatcagc atcgacctg aaccgaaat 
      ctcatcgaa ttcatctcg ccacacgtc tttcaggga 
      cacagtttt cgggaaatt gatttgagc ttcaatcct 
      gttaccata tcggaggat ttttttaga agtgaattg 
      gaaattaaa actttcgtt tttaatttg gattattta 
      catcatggg actgtggaa aaacttcga tacattgca 
      ggaagtttc aggcctctt gtgaggtgt tgccctctg 
      gcggcctgt cccccagct gccgtccga cgcctgcgt 
      gaacgacgg cgctctcaa ccgcgcccg atggacgcc 
      agtacactc tcagccatg gataaggac gctctcaag 
      aagcagata gagaacatg aaataccaa gcatcaatg 
      gaacgctgg cccttgtcg aaatcgatc gccgcgatg 
      agggaatac gtggaggag aacgaaaag aacgacccg 
      ctgatccac gcccccgac aagaaaaac aacccatgg 
      gcggaaaaa gggaaatgc gtcattatg tag
    mRNA sequence
    • >TC033349 RA
      taatatgct attatctta ctggtgcag ttttctaga 
      tacgttata caattgttt tcgcaatgc gacttttgt 
      attttaaca cgtttgttc tctattctt tcttcggtt 
      atgtgtata atgaagaaa aatcactcc agtattcaa 
      tttacgacg aagaaacca acatggtca taacaacaa 
      ttaactcaa ctgtcgtgg aaatcgaag aagacacta 
      ctttagtaa atgtcgact tgcaagaga acacaaaat 
      tgtgcaaca aaatgttca atctgtctt cgaaagtcg 
      agtctttaa ttattacag gcccccatg ttgtggaaa 
      ttgagacaa attttttac actcccaga acgtatacg 
      agctctcta tttcaaaca ctggaataa ctacgatca 
      aaaaataca ctttcaaaa atctaaaaa tacaaactt 
      tagaattgt ttgataacg aaattcaaa cgcttgaag 
      ctaaagcat ttgccgact tattccagt tacataaac 
      tggacttgt ccaacaacg atttaacga cgttggacc 
      tagaaagtt tctacagac tgccccttc ttgattact 
      ttgatgcta aagggaatc aaatcgtga aattgggaa 
      aacagtttt tccaaattt tgcgcaagg attattccg 
      aaatgtggc tttgtagca acaaaatcc aagttctag 
      gtgtggagt ttttcgaag gtttgggtg taaataaca 
      tcagcatcg acctgaacc gaaatctca tcgaattca 
      tctcgccac acgtctttc agggacaca gttttcggg 
      aaattgatt tgagcttca atcctgtta ccatatcgg 
      aggattttt ttagaagtg aattggaaa ttaaaactt 
      tcgttttta atttggatt atttacatc atgggactg 
      tggaaaaac ttcgataca ttgcaggaa gtttcaggc 
      ctcttgtga ggtgttgcc ctctggcgg cctgtcccc 
      cagctgccg tccgacgcc tgcgtgaac gacggcgct 
      ctcaaccgc gcccgatgg acgccagta cactctcag 
      ccatggata aggacgctc tcaagaagc agatagaga 
      acatgaaat accaagcat caatggaac gctggccct 
      tgtcgaaat cgatcgccg cgatgaggg aatacgtgg 
      aggagaacg aaaagaacg acccgctga tccacgccc 
      ccgacaaga aaaacaacc catgggcgg aaaaaggga 
      aatgcgtca ttatgtaga atttcatgc agctgctta 
      ttagtttgt tcatattga ttttcccat tgcttctgt 
      tcatctacc ttaatttca acagtttga tgttagatg 
      ggataacca cactgttct atactttta aaatttcaa 
      aaatcgaag cgtcatcag gtctgtgaa tgttttttc 
      ggaaaatgg gacactcca cgagccttc cgggcgaat 
      tgttaacat tattgttat gtagtttaa acatactca 
      cagttttgt gtacgaaat gtaagatct caaaagtgt 
      gttaaattt ggattcgtg tcaacggcc ggtttctgg 
      ttttggcga accagaggc ggccaccaa aataaacaa 
      tcaattttt ttacaagcg aactctcgc ttttcgttt 
      cagtctctt gggtcttat tatttgaag tctgatgtt 
      gttattcct atttttgtt gacttaatt ttaataaat 
      ctctaattg taattttag acttaacat tcttagtct 
      agtcaatac cgaaatttt gggccattg caaccaacg 
      aaacgactc actccctgg ctcaagaaa ctcaaattt 
      ggaccaaac cggctggta ctttaccga agacgacaa 
      aaaagagca acttccgtt taccatcca cgggtctct 
      tcggtaata ctgccatct gtacttggc tgtggtttg 
      atccaactt tgacctctc cattctggt aatagtctg 
      acagtgcaa tttaaatgt gacttttaa atttcgcgc 
      gaaagcgca ttaaaatga cacgacacg tgaccagga 
      cggagtacc acgtgacgc tccctccta gccacattt 
      ttaaacact cgactaaaa acgattgac taaacatta 
      agagagata gatgactgt catgactta aaatctcga 
      ctatgactc aacatttaa ctttaagga gctttagct 
      ttgtttagt tccccgatg tcttcccga gatagcgtt 
      gtgtaacgc gggacagtt gaattttaa atgtgtaac 
      ctactccag gcactggac tagttttaa cgatataac 
      taaaattag actaaaagt taaacactt gaagtgttt 
      ttaaaataa tattatcaa atttaaatt aataataat 
      agaacccgt agttaaaaa atatatgat aatcaaacc 
      taatattta aattagcat aggttctat taatgtatg 
      acacctaat aatcaaaca gtgcgttca ataattacc 
      tccccacga aaatatcac tcttcactt agaaaaaat 
      gtaacacta tgtcttaat aattgttat cttatagac 
      taatttctt aactctaag tattatcga aaggatttg 
      acttcttta ctcctgtac taatttcca agggtagcc 
      gggtcgtgt cgttggttg cgatcgctc agagtttcg 
      tttttattt atatgagag aaattacta ttagtaaaa 
      gatacttca attaaacct tgtaatttt aaatatgta 
      atgtggtaa tacttgaga taataaact ccataaaag 
      aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY