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Gene information
General information
TC015465 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9551152..9554513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015465
    iBeetle screen iB_09151

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015465 RA
      MKNLNIVFV ANILPLLLA ITPTKCVLN EQELKNLLA 
      NEYERTASA ICTKVNEAE WNYETDINN HEKEQIVLN 
      ATLQSAAFH KKYWNKYFK DLKPEDFQD PEIKRQVKD 
      LKLLGDSAL DEKKLAELT KTVNAMTNI YSTAKICPY 
      KDQQCDLAK SGLSLEPGI EAVIAKSTD YDELLYVWK 
      AWRDASGAK MRNLYRIYV DLSNEAAVA NNFKDKGEV 
      WRYGYESKT FIQDIDELW NQVEPLYLE LHKYVGSKL 
      KERFGDKLD DSDGLLPAH LFGNMWAQE WNNIAQLVK 
      PFPNASKID VDKALIEQK YTILDLFQT SDNFYKSLG 
      LIPSDICYN TSAGAMIEK PTDGREVLC HASAWDFCD 
      GKNYRIKMC TEVNFEDFI TIHHEMGHI QYFLQYAKQ 
      PITFREGAN PGFHEAIGD TIALSVSTP KHLEKINLL 
      KNYNNSYES SINTLMDMA LQKIAFLPF GLLIDKWRW 
      DVFSGAVKP EQWNAHWWE YRKKYQKIK PPVARSEED 
      FDPGAKFHV AGDSEYIAY FVAHTLQFQ FYKSLCVEA 
      GEYNPDDKN VPLHNCDFY ESKAAGDKL REGLSLGSS 
      KHWSEVLEV MTGSRKLDA SAVLEYFEP LYKFLKEKN 
      SK
    CDS sequence
    • >TC015465 RA
      atgaaaaat ttaaacatc gtttttgtg gcaaatatt 
      ctgccactt ttacttgcc atcactccg acaaaatgt 
      gttctaaac gaacaagaa ctcaaaaat ttgttggct 
      aatgaatac gaaagaacc gcctcagca atttgcaca 
      aaggtgaac gaagccgaa tggaattac gaaactgat 
      ataaataat cacgaaaag gaacaaatc gtgttaaac 
      gcgacgcta caatcggca gcattccac aaaaaatac 
      tggaataaa tatttcaaa gacctaaaa cctgaagac 
      ttccaagat ccggaaatc aaaagacag gtcaaagac 
      ctgaaactt ttaggtgat agtgcactt gatgagaaa 
      aaattggca gaattaact aaaacggtc aatgcaatg 
      actaatatt tacagtacg gcgaaaatt tgcccttat 
      aaggaccaa caatgcgat ttagccaaa tctggactg 
      tctttagag ccaggaatt gaagcagtt attgccaaa 
      tctacggat tatgacgag ctgttgtac gtttggaag 
      gcttggaga gatgcttca ggtgccaaa atgaggaac 
      ttgtaccgg atttatgtc gatttgtcc aacgaagcc 
      gccgttgca aacaatttt aaggacaaa ggggaggta 
      tggaggtac ggttacgag tccaaaacc ttcatccaa 
      gacattgac gagctttgg aatcaagtt gagccactc 
      tatcttgag ttacataaa tatgtgggc agtaaattg 
      aaggaacgt ttcggtgac aaacttgat gatagtgat 
      ggtcttctc cccgcccat ttattcggt aatatgtgg 
      gcccaagaa tggaataac atagcccag ctggtgaaa 
      ccattcccc aatgcgagt aaaatcgac gtagacaag 
      gctttaatt gaacagaaa tacacaatt ttggacttg 
      tttcaaacc tctgataac ttttacaaa tctttaggg 
      ctcatacct agcgatatt tgctacaat actagtgct 
      ggcgcaatg attgagaaa ccaaccgat ggtagagaa 
      gtgctatgt catgccagt gcttgggat ttctgcgat 
      gggaaaaac tacaggatt aaaatgtgc actgaagtg 
      aatttcgaa gactttatt acaatccac cacgaaatg 
      ggtcacata cagtatttc ctccagtat gccaaacaa 
      cccatcacg ttccgtgag ggggccaat ccaggcttc 
      catgaagcc ataggcgat accatcgcc ttgtcggtt 
      tccacgcca aaacatttg gaaaaaatc aatcttttg 
      aaaaactat aacaacagt tatgagtcc tcgatcaat 
      acacttatg gacatggcg ttgcaaaaa atcgccttt 
      ttgcccttt ggactactg attgataag tggagatgg 
      gatgttttc agcggagct gtgaaacca gaacagtgg 
      aacgcacac tggtgggag tacaggaaa aagtaccag 
      aaaatcaaa ccgccggta gcaaggagt gaggaggat 
      tttgatccc ggtgctaag ttccacgtg gctggagac 
      tcggaatat atagcttat tttgtggcc catactttg 
      cagtttcaa ttttacaaa agtttgtgt gtggaagct 
      ggggaatat aatccagat gataagaat gtgccgtta 
      cataattgc gatttttac gaatctaaa gcagcagga 
      gataaatta agggaaggt ttgtcgctc ggttctagc 
      aaacactgg agtgaggtt ttggaagtt atgactggg 
      agtagaaag ttggatgct tcggctgtt ttggagtac 
      tttgaaccg ttgtataag tttttaaaa gagaaaaat 
      tcgaaataa
    mRNA sequence
    • >TC015465 RA
      attgtaatg tattttctt gtggttatc aatacctgc 
      ttcctgctg gctttataa gagctcctg acaaagttt 
      cttacttaa aacaacctg catgttaga acggtgacc 
      actcagaac tagtcgatc gaagagagc agaaacagt 
      gttgtgatt tcatgtagt tttaaaatg aaaaattta 
      aacatcgtt tttgtggca aatattctg ccactttta 
      cttgccatc actccgaca aaatgtgtt ctaaacgaa 
      caagaactc aaaaatttg ttggctaat gaatacgaa 
      agaaccgcc tcagcaatt tgcacaaag gtgaacgaa 
      gccgaatgg aattacgaa actgatata aataatcac 
      gaaaaggaa caaatcgtg ttaaacgcg acgctacaa 
      tcggcagca ttccacaaa aaatactgg aataaatat 
      ttcaaagac ctaaaacct gaagacttc caagatccg 
      gaaatcaaa agacaggtc aaagacctg aaactttta 
      ggtgatagt gcacttgat gagaaaaaa ttggcagaa 
      ttaactaaa acggtcaat gcaatgact aatatttac 
      agtacggcg aaaatttgc ccttataag gaccaacaa 
      tgcgattta gccaaatct ggactgtct ttagagcca 
      ggaattgaa gcagttatt gccaaatct acggattat 
      gacgagctg ttgtacgtt tggaaggct tggagagat 
      gcttcaggt gccaaaatg aggaacttg taccggatt 
      tatgtcgat ttgtccaac gaagccgcc gttgcaaac 
      aattttaag gacaaaggg gaggtatgg aggtacggt 
      tacgagtcc aaaaccttc atccaagac attgacgag 
      ctttggaat caagttgag ccactctat cttgagtta 
      cataaatat gtgggcagt aaattgaag gaacgtttc 
      ggtgacaaa cttgatgat agtgatggt cttctcccc 
      gcccattta ttcggtaat atgtgggcc caagaatgg 
      aataacata gcccagctg gtgaaacca ttccccaat 
      gcgagtaaa atcgacgta gacaaggct ttaattgaa 
      cagaaatac acaattttg gacttgttt caaacctct 
      gataacttt tacaaatct ttagggctc atacctagc 
      gatatttgc tacaatact agtgctggc gcaatgatt 
      gagaaacca accgatggt agagaagtg ctatgtcat 
      gccagtgct tgggatttc tgcgatggg aaaaactac 
      aggattaaa atgtgcact gaagtgaat ttcgaagac 
      tttattaca atccaccac gaaatgggt cacatacag 
      tatttcctc cagtatgcc aaacaaccc atcacgttc 
      cgtgagggg gccaatcca ggcttccat gaagccata 
      ggcgatacc atcgccttg tcggtttcc acgccaaaa 
      catttggaa aaaatcaat cttttgaaa aactataac 
      aacagttat gagtcctcg atcaataca cttatggac 
      atggcgttg caaaaaatc gcctttttg ccctttgga 
      ctactgatt gataagtgg agatgggat gttttcagc 
      ggagctgtg aaaccagaa cagtggaac gcacactgg 
      tgggagtac aggaaaaag taccagaaa atcaaaccg 
      ccggtagca aggagtgag gaggatttt gatcccggt 
      gctaagttc cacgtggct ggagactcg gaatatata 
      gcttatttt gtggcccat actttgcag tttcaattt 
      tacaaaagt ttgtgtgtg gaagctggg gaatataat 
      ccagatgat aagaatgtg ccgttacat aattgcgat 
      ttttacgaa tctaaagca gcaggagat aaattaagg 
      gaaggtttg tcgctcggt tctagcaaa cactggagt 
      gaggttttg gaagttatg actgggagt agaaagttg 
      gatgcttcg gctgttttg gagtacttt gaaccgttg 
      tataagttt ttaaaagag aaaaattcg aaataaaaa 
      atgtgttaa tttgtcgta atttcccgt ctttttttt 
      atttctaga aaaattaag atagacact gctaattac 
      tagtgtttc aaaaaaact gtttttcaa aaacaatta 
      ctcctaatt tttgagacc ttgccaatg ctacaccta 
      ctagattaa attagcata gtattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY