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Gene information
General information
TC015272 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9698617..9699955
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015272
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09149

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Nervous system outside of larvae
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    procephalic appendages not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015272 RA
      MDVQSIPFL SEVVPLDPS SSIQEHKVR RLTAAGENY 
      GSLMLSVDI TVKSPEGTR EINAVAKMV PPNEYIQKV 
      FNTQVTFRN EIAFYREIV PTLRRFQQE QGMANAIDF 
      FPKYLGSRL NRDNSDRVD SDGILLLEN LKMAGYANL 
      ERTVGFDLE VAKVVVKDL AHLHAVPLG LKLQKPDVF 
      EKKVKKYLS PFHPDDDEK IVEKMCNLV CSLDTCQKY 
      HERAKSALW KMRDRNTTI REPFATLVH DDCWVNNTM 
      VKIQPGEVP KNKFVDFQI CNYGSPAKD LLFLLFSSV 
      QNDVIRSEC DNLIRLYHG TFVDVLRQL KCDTEPFSF 
      EKFEQEIKF EALNTQFGH VVFMLTPIF APKGSVKEL 
      DEFSPEAFA EMVGSFASD LHKEKLALV VNEFGKRNW 
      I
    CDS sequence
    • >TC015272 RA
      atggacgtg caatcaatc ccgttcctc tccgaagtg 
      gtccctctg gacccttcc tccagcatc caggagcac 
      aaagtgcgc cgtttaacc gcagctggc gaaaactac 
      ggcagtctg atgctcagt gtggacatc acagtcaaa 
      agtcccgag ggaacacgc gaaataaac gccgtagcc 
      aaaatggtg ccacccaac gaatacatc cagaaggtc 
      ttcaacacc caggtgacc ttccggaac gaaatcgcc 
      ttctacagg gagatagtg ccgacattg cgacgcttc 
      caacaggag caaggcatg gctaacgcg atcgacttc 
      ttcccgaaa tatttaggg agtcgcttg aatcgggat 
      aattcggat cgggtcgac tcggacggc attttgttg 
      cttgagaac ctcaaaatg gcgggctac gccaacttg 
      gagcgcacg gtagggttc gaccttgag gttgctaag 
      gtggttgtc aaggacttg gcgcatttg catgccgtt 
      ccgttgggc cttaaatta caaaaacca gacgttttt 
      gaaaaaaaa gtcaaaaaa tatctatcg ccctttcat 
      ccagacgat gacgaaaaa attgtggaa aaaatgtgc 
      aatttggtt tgtagtctt gacacttgt caaaaatac 
      cacgaaagg gccaaatcg gctctttgg aaaatgcgg 
      gacagaaat acaacaatc agagagccc tttgcaact 
      ttggtgcat gatgattgt tgggtcaac aacaccatg 
      gtgaagatc cagccgggg gaagtcccc aaaaataaa 
      ttcgttgat ttccaaatt tgcaactac gggtcacca 
      gccaaggat ttgctcttt ttgctattc tccagtgtt 
      caaaatgat gtgattagg agcgagtgt gataatttg 
      atcagactg tatcatggg acgtttgtt gacgttttg 
      agacagctc aagtgtgac actgaaccg ttcagtttt 
      gaaaaattc gagcaagag atcaagttt gaagcgtta 
      aatacgcaa tttggacac gttgtcttt atgttaacg 
      ccgattttt gccccaaaa ggcagtgtg aaagaactg 
      gacgagttt tcaccagag gcgtttgcg gaaatggtc 
      ggttcgttt gcttccgat ttgcataag gagaagttg 
      gcgttggtt gtgaacgaa tttggcaaa agaaactgg 
      atttaa
    mRNA sequence
    • >TC015272 RA
      tcacaaata attgcaaat aaaaaaccg atcagttct 
      taaaaaatc cggcaatgg acgtgcaat caatcccgt 
      tcctctccg aagtggtcc ctctggacc cttcctcca 
      gcatccagg agcacaaag tgcgccgtt taaccgcag 
      ctggcgaaa actacggca gtctgatgc tcagtgtgg 
      acatcacag tcaaaagtc ccgagggaa cacgcgaaa 
      taaacgccg tagccaaaa tggtgccac ccaacgaat 
      acatccaga aggtcttca acacccagg tgaccttcc 
      ggaacgaaa tcgccttct acagggaga tagtgccga 
      cattgcgac gcttccaac aggagcaag gcatggcta 
      acgcgatcg acttcttcc cgaaatatt tagggagtc 
      gcttgaatc gggataatt cggatcggg tcgactcgg 
      acggcattt tgttgcttg agaacctca aaatggcgg 
      gctacgcca acttggagc gcacggtag ggttcgacc 
      ttgaggttg ctaaggtgg ttgtcaagg acttggcgc 
      atttgcatg ccgttccgt tgggcctta aattacaaa 
      aaccagacg tttttgaaa aaaaagtca aaaaatatc 
      tatcgccct ttcatccag acgatgacg aaaaaattg 
      tggaaaaaa tgtgcaatt tggtttgta gtcttgaca 
      cttgtcaaa aataccacg aaagggcca aatcggctc 
      tttggaaaa tgcgggaca gaaatacaa caatcagag 
      agccctttg caactttgg tgcatgatg attgttggg 
      tcaacaaca ccatggtga agatccagc cgggggaag 
      tccccaaaa ataaattcg ttgatttcc aaatttgca 
      actacgggt caccagcca aggatttgc tctttttgc 
      tattctcca gtgttcaaa atgatgtga ttaggagcg 
      agtgtgata atttgatca gactgtatc atgggacgt 
      ttgttgacg ttttgagac agctcaagt gtgacactg 
      aaccgttca gttttgaaa aattcgagc aagagatca 
      agtttgaag cgttaaata cgcaatttg gacacgttg 
      tctttatgt taacgccga tttttgccc caaaaggca 
      gtgtgaaag aactggacg agttttcac cagaggcgt 
      ttgcggaaa tggtcggtt cgtttgctt ccgatttgc 
      ataaggaga agttggcgt tggttgtga acgaatttg 
      gcaaaagaa actggattt aaataaaaa aatttgaac 
      taaaggagt ttcattatt atgttatgc actattttt 
      tctacctcc ccctctttt tttggaata aacttattt 
      tttattc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY