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Gene information
General information
TC015272 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9698617..9699955
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015272
    iBeetle screen iB_09149

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Nervous system outside of larvae
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     procephalic appendages not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015272 RA
      MDVQSIPFL SEVVPLDPS SSIQEHKVR RLTAAGENY 
GSLMLSVDI TVKSPEGTR EINAVAKMV PPNEYIQKV 
FNTQVTFRN EIAFYREIV PTLRRFQQE QGMANAIDF 
FPKYLGSRL NRDNSDRVD SDGILLLEN LKMAGYANL 
ERTVGFDLE VAKVVVKDL AHLHAVPLG LKLQKPDVF 
EKKVKKYLS PFHPDDDEK IVEKMCNLV CSLDTCQKY 
HERAKSALW KMRDRNTTI REPFATLVH DDCWVNNTM 
VKIQPGEVP KNKFVDFQI CNYGSPAKD LLFLLFSSV 
QNDVIRSEC DNLIRLYHG TFVDVLRQL KCDTEPFSF 
EKFEQEIKF EALNTQFGH VVFMLTPIF APKGSVKEL 
DEFSPEAFA EMVGSFASD LHKEKLALV VNEFGKRNW 
I
    CDS sequence
    • >TC015272 RA
      atggacgtg caatcaatc ccgttcctc tccgaagtg 
gtccctctg gacccttcc tccagcatc caggagcac 
aaagtgcgc cgtttaacc gcagctggc gaaaactac 
ggcagtctg atgctcagt gtggacatc acagtcaaa 
agtcccgag ggaacacgc gaaataaac gccgtagcc 
aaaatggtg ccacccaac gaatacatc cagaaggtc 
ttcaacacc caggtgacc ttccggaac gaaatcgcc 
ttctacagg gagatagtg ccgacattg cgacgcttc 
caacaggag caaggcatg gctaacgcg atcgacttc 
ttcccgaaa tatttaggg agtcgcttg aatcgggat 
aattcggat cgggtcgac tcggacggc attttgttg 
cttgagaac ctcaaaatg gcgggctac gccaacttg 
gagcgcacg gtagggttc gaccttgag gttgctaag 
gtggttgtc aaggacttg gcgcatttg catgccgtt 
ccgttgggc cttaaatta caaaaacca gacgttttt 
gaaaaaaaa gtcaaaaaa tatctatcg ccctttcat 
ccagacgat gacgaaaaa attgtggaa aaaatgtgc 
aatttggtt tgtagtctt gacacttgt caaaaatac 
cacgaaagg gccaaatcg gctctttgg aaaatgcgg 
gacagaaat acaacaatc agagagccc tttgcaact 
ttggtgcat gatgattgt tgggtcaac aacaccatg 
gtgaagatc cagccgggg gaagtcccc aaaaataaa 
ttcgttgat ttccaaatt tgcaactac gggtcacca 
gccaaggat ttgctcttt ttgctattc tccagtgtt 
caaaatgat gtgattagg agcgagtgt gataatttg 
atcagactg tatcatggg acgtttgtt gacgttttg 
agacagctc aagtgtgac actgaaccg ttcagtttt 
gaaaaattc gagcaagag atcaagttt gaagcgtta 
aatacgcaa tttggacac gttgtcttt atgttaacg 
ccgattttt gccccaaaa ggcagtgtg aaagaactg 
gacgagttt tcaccagag gcgtttgcg gaaatggtc 
ggttcgttt gcttccgat ttgcataag gagaagttg 
gcgttggtt gtgaacgaa tttggcaaa agaaactgg 
atttaa
    mRNA sequence
    • >TC015272 RA
      tcacaaata attgcaaat aaaaaaccg atcagttct 
taaaaaatc cggcaatgg acgtgcaat caatcccgt 
tcctctccg aagtggtcc ctctggacc cttcctcca 
gcatccagg agcacaaag tgcgccgtt taaccgcag 
ctggcgaaa actacggca gtctgatgc tcagtgtgg 
acatcacag tcaaaagtc ccgagggaa cacgcgaaa 
taaacgccg tagccaaaa tggtgccac ccaacgaat 
acatccaga aggtcttca acacccagg tgaccttcc 
ggaacgaaa tcgccttct acagggaga tagtgccga 
cattgcgac gcttccaac aggagcaag gcatggcta 
acgcgatcg acttcttcc cgaaatatt tagggagtc 
gcttgaatc gggataatt cggatcggg tcgactcgg 
acggcattt tgttgcttg agaacctca aaatggcgg 
gctacgcca acttggagc gcacggtag ggttcgacc 
ttgaggttg ctaaggtgg ttgtcaagg acttggcgc 
atttgcatg ccgttccgt tgggcctta aattacaaa 
aaccagacg tttttgaaa aaaaagtca aaaaatatc 
tatcgccct ttcatccag acgatgacg aaaaaattg 
tggaaaaaa tgtgcaatt tggtttgta gtcttgaca 
cttgtcaaa aataccacg aaagggcca aatcggctc 
tttggaaaa tgcgggaca gaaatacaa caatcagag 
agccctttg caactttgg tgcatgatg attgttggg 
tcaacaaca ccatggtga agatccagc cgggggaag 
tccccaaaa ataaattcg ttgatttcc aaatttgca 
actacgggt caccagcca aggatttgc tctttttgc 
tattctcca gtgttcaaa atgatgtga ttaggagcg 
agtgtgata atttgatca gactgtatc atgggacgt 
ttgttgacg ttttgagac agctcaagt gtgacactg 
aaccgttca gttttgaaa aattcgagc aagagatca 
agtttgaag cgttaaata cgcaatttg gacacgttg 
tctttatgt taacgccga tttttgccc caaaaggca 
gtgtgaaag aactggacg agttttcac cagaggcgt 
ttgcggaaa tggtcggtt cgtttgctt ccgatttgc 
ataaggaga agttggcgt tggttgtga acgaatttg 
gcaaaagaa actggattt aaataaaaa aatttgaac 
taaaggagt ttcattatt atgttatgc actattttt 
tctacctcc ccctctttt tttggaata aacttattt 
tttattc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY