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Gene information
General information
TC014215 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3319019..3321303
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014215
    iBeetle screen iB_09148

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014215 RA
      MKIYCNIVI FIISVLVGL LCILISVIQ YYYLNYNFY 
      KKHTSYAPI FEDLIATDS NNNFALSYT FNAIEDHSN 
      DNFILSNLN ALLESHYVP TTGSTYLDG SRNFNVKST 
      SNSEVLNIL QAAIGFQLA GKNHKALKL LEHATAIAP 
      DNPDVLNWY GEFLEQIRH DVVTADELY FKALTHSPT 
      HAAALTNHK RTSPVVDRL DLELFKSID KKKDYLKKQ 
      FDSYNFETL KKQAYYLHI YHTVGIEGN TMTVEQLRT 
      LLETGQVIQ GKSIIEHNE VLGLQLALK YVKSLARKE 
      YITVDDILQ MHKRVMGHV DPLTSGIFR DTKVFVGRH 
      IPPPSEKLP ALMESYVNW LNSDEARNM HPVRYAALA 
      HYKLVDIHP FVDGNGRTS RLIMNLILL RGGYPPIMI 
      LKEQRDRYY DALNVANVG DVRPFVRFI AHCTLQILD 
      MYIHGTQML VLDGDSTEG TPTPFLT
    CDS sequence
    • >TC014215 RA
      atgaagatt tattgcaac attgtaatt tttataatt 
      tcggtgcta gttggttta ttgtgcatt ttaatttct 
      gttatacaa tattactat ttaaactac aacttttac 
      aagaaacat acgagttat gcgccaatt ttcgaggat 
      ttgattgcg acagactcg aacaacaat ttcgcatta 
      agttacacc tttaacgcc attgaggac cactcaaac 
      gacaacttt attctaagt aatttaaac gctcttctc 
      gaatctcac tatgtgcca acgactggt agcacgtac 
      ttagacggg agtcgaaat tttaacgtg aaatcaacc 
      tccaattcg gaagttttg aacatattg caggcggct 
      atcgggttc caactcgct gggaaaaac cacaaggct 
      ttgaagtta ctggaacat gcaacggcc atcgccccg 
      gataatcca gacgttttg aactggtac ggggagttc 
      cttgagcaa atccgacac gatgtcgtg acagcggat 
      gagctctac ttcaaagca ctaacccac tctcccaca 
      cacgcagcc gcgctcacc aaccacaaa agaacctca 
      cccgtggtg gaccgatta gacttggag cttttcaaa 
      agcatagat aaaaagaag gattatttg aagaaacaa 
      ttcgatagt tacaacttc gaaactttg aaaaagcag 
      gcgtactat ttgcacatt taccacact gtggggatt 
      gagggcaac accatgacc gtcgagcaa ctgcggact 
      ttgctggag actggtcag gtgatccaa ggcaagagc 
      ataattgaa cataacgaa gtactgggg ctgcagctg 
      gccctcaag tacgtgaaa agtctggcc aggaaggag 
      tacatcacc gttgatgac atcctccaa atgcataaa 
      cgcgtgatg gggcacgtg gatccccta acatccggg 
      attttccgc gatacgaaa gtttttgtg ggccgccac 
      atcccaccg ccgtccgag aaactccca gcgctgatg 
      gagagctac gtgaattgg ctgaactcg gacgaggcc 
      cggaacatg caccccgtg cgctacgcc gctctggcg 
      cactacaag ctggtcgac atccacccg tttgttgac 
      ggcaacgga cgcacaagt cggctgatc atgaacttg 
      attttgctc cggggcggc tacccgccc attatgatt 
      ttgaaagag cagagggat cggtactac gatgccttg 
      aatgtggcc aacgtgggg gacgtgcgg cctttcgtc 
      cgatttata gcgcactgt acgttacaa atcctagat 
      atgtacatt cacggcaca caaatgttg gtgctggac 
      ggggacagc actgaaggg accccgacc ccgttcctg 
      acctga
    mRNA sequence
    • >TC014215 RA
      aaacgtcaa aatgtggga ggcaaacat aacctgaaa 
      ctgatatca tttctttta cgtgtaatt actttttat 
      cactcaatg ttttataat ggaagataa tttaggacc 
      gtttaataa aatttggga agacaccta atatgaaga 
      tttattgca acattgtaa tttttataa tttcggtgc 
      tagttggtt tattgtgca ttttaattt ctgttatac 
      aatattact atttaaact acaactttt acaagaaac 
      atacgagtt atgcgccaa ttttcgagg atttgattg 
      cgacagact cgaacaaca atttcgcat taagttaca 
      cctttaacg ccattgagg accactcaa acgacaact 
      ttattctaa gtaatttaa acgctcttc tcgaatctc 
      actatgtgc caacgactg gtagcacgt acttagacg 
      ggagtcgaa attttaacg tgaaatcaa cctccaatt 
      cggaagttt tgaacatat tgcaggcgg ctatcgggt 
      tccaactcg ctgggaaaa accacaagg ctttgaagt 
      tactggaac atgcaacgg ccatcgccc cggataatc 
      cagacgttt tgaactggt acggggagt tccttgagc 
      aaatccgac acgatgtcg tgacagcgg atgagctct 
      acttcaaag cactaaccc actctccca cacacgcag 
      ccgcgctca ccaaccaca aaagaacct cacccgtgg 
      tggaccgat tagacttgg agcttttca aaagcatag 
      ataaaaaga aggattatt tgaagaaac aattcgata 
      gttacaact tcgaaactt tgaaaaagc aggcgtact 
      atttgcaca tttaccaca ctgtgggga ttgagggca 
      acaccatga ccgtcgagc aactgcgga ctttgctgg 
      agactggtc aggtgatcc aaggcaaga gcataattg 
      aacataacg aagtactgg ggctgcagc tggccctca 
      agtacgtga aaagtctgg ccaggaagg agtacatca 
      ccgttgatg acatcctcc aaatgcata aacgcgtga 
      tggggcacg tggatcccc taacatccg ggattttcc 
      gcgatacga aagtttttg tgggccgcc acatcccac 
      cgccgtccg agaaactcc cagcgctga tggagagct 
      acgtgaatt ggctgaact cggacgagg cccggaaca 
      tgcaccccg tgcgctacg ccgctctgg cgcactaca 
      agctggtcg acatccacc cgtttgttg acggcaacg 
      gacgcacaa gtcggctga tcatgaact tgattttgc 
      tccggggcg gctacccgc ccattatga ttttgaaag 
      agcagaggg atcggtact acgatgcct tgaatgtgg 
      ccaacgtgg gggacgtgc ggcctttcg tccgattta 
      tagcgcact gtacgttac aaatcctag atatgtaca 
      ttcacggca cacaaatgt tggtgctgg acggggaca 
      gcactgaag ggaccccga ccccgttcc tgacctgac 
      actgtacct atactgtta agcgggttg caatcaaac 
      gattcgtaa aaatgcggt cattttatt atggtaatt 
      ataatgaat gttcaagct tgttatttt ataaattta 
      aaagcaaac acaatctgg tttttaaaa caaacttga 
      tgaattaca agtaattaa taacatgta tatgacgac 
      tctaaaacc aacaaaagt ttttacaac caacaaaag 
      tttttacaa ggtattgag tagaaggaa agcagggca 
      ttaaccgtc gtttaacga actgttttc tctactcac 
      aaacaaggc ctcttaaat tatgcacct catgatttt 
      gtaacatta ctaaagcgc taacgtgaa acaaagtga 
      cgtgatagc ctgcatttg ttgtaaaaa aaatatata 
      tagcacagc ctaatattt aatacaaat taatgataa 
      tgatggata agtcgaaat ttcgcacat cgtcggaat 
      gtgatccga attagttga aattattaa agcgtacgt 
      tacggccac gtcccacct attaagagg cctccaaat 
      aaattaatt gcaaaaata aaaaaggta ataaataaa 
      aattagtat caaaacaac agcctcaga gaaatagaa 
      gtcgtttcc aaagcgtgc agttctttt tgtaaattt 
      gaataacat aataaataa acagaacta gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY