Status
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Gene information
General information
TC015420 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 635355..643862
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015420
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09145

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    egg chamber amount decreased - Number of animals showing the phenotype: 4

    egg chamber potentiallydegenerated - Number of animals showing the phenotype: 4
    yolk fragmented - Number of animals showing the phenotype: 4

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015420 RA
      MTSKVQHVQ DNSVAERRL RPIYDWLDI GNNKKALQE 
      CEKVLKKTP NLLCAKALK ALTLHRMGK EGEALAILE 
      ALTADSPTD DATLQAMTL CYRELQQLE KICKLYETA 
      VKLEPANEE LYTHLFMSY ARVFDFKSQ QKTAMTLYK 
      HKPKTPYYC WAVMSIILQ ATRGEGKND AKKRSLLLS 
      LAERMMEKL IVDGKLDAE QEVQLYVMV LELQGKFEE 
      VLVILDGPL GTKLACSNQ PHNKLKYLV PLKCWDDVN 
      LLCKGILIE SVDRWDIWK EYINSVFAL MEAKPYESN 
      NHDNGASDN DLETVDDTP EKCHEFICR IVENGADNG 
      FLLRGPYLA RFELCSRLV KCNVDSSDV LGETIELFI 
      EYFRKFGHK PCCVSDLRH YLDLLDCEK KAELSARLI 
      KDVGISSTS VPQSEQQMQ RHLCALQLS RLCGAHRNL 
      TSDHLKALV TAFSLHYEH GYQTYGTNL LSTDLGPSD 
      PYALLAAHV LYDLSQSEK KSEPIVAAL VLLENMLKN 
      SPSNFHAKL LCVRLYHTV GGCLGAQNM YSSLDTKHL 
      QLDSLGFIH CARLATTGL YSLCSNLFD VTLKFFSSN 
      YKDSSDHIT FSYKYGSFI KLDEFMDFR ERLNNSMHY 
      TTVAIDRIV LSLVECTTL DTLYNIDIS PKDDKIDWE 
      ALRDNHDLS VYISWDPER IKGPPEEYE EIRTFFKQN 
      KIFLKLRTS LLWALSAAV DIVKSSDGV RTKYMDQLA 
      TILADWEEL STKNRNHAP LCQHLVTLP LPSRLHVAL 
      ATPHFDIVG RMLRLLLAV VSEGCSKED DLVEKIESG 
      VKAMVDMIE NRGKNSADL IYRRETMEY VVNAVEVLS 
      VSSLICLLC NDLVKPPSK KGKKKVSEA SSWKETVVR 
      LANSFKTHI NALNEVLQT WAQFSVSSD LALMLESVN 
      LSNDGHNVC ENIVNSYVL AVREIQCVL KNKIKMLSS 
      IQ
    CDS sequence
    • >TC015420 RA
      atgacttcg aaggtccaa cacgtgcag gacaactca 
      gtagccgaa cggagattg cgccccatt tacgattgg 
      ctggacatc ggcaacaac aaaaaagcg ctccaggag 
      tgcgagaaa gtgctgaaa aagacgccg aatttgctg 
      tgcgcgaaa gcgctcaaa gccctgacg ctgcaccgg 
      atgggcaag gagggcgaa gccctggcc atcctggag 
      gccctgact gccgacagc cccacggac gacgccacg 
      ctccaagcc atgactctg tgctacaga gagttgcaa 
      caacttgaa aaaatttgt aaattgtac gagactgcg 
      gtcaagttg gagccggca aatgaggag ctttacacg 
      catttattt atgtcttat gcgcgtgtt ttcgatttt 
      aaatcgcaa cagaaaacg gcaatgacg ctgtacaag 
      cacaagccc aaaacgccg tactattgc tgggctgtt 
      atgagtatt attttacaa gccacgcgt ggcgagggc 
      aagaacgat gcgaaaaaa cgcagttta ttgctgagt 
      ctggccgaa cgtatgatg gaaaagttg attgtcgat 
      gggaaactt gatgcggaa caggaagtt caactgtat 
      gttatggtt ttggagctg cagggcaag tttgaggag 
      gttttggtt attttggat gggcctctc ggtaccaag 
      ctggcttgt agtaatcag ccccataat aagctgaag 
      tatttggtg cccctcaag tgctgggat gatgttaat 
      ttgttgtgt aaggggatc ttgattgag agtgtagac 
      aggtgggat atttggaag gaatacatc aactcggtg 
      tttgcccta atggaggcc aaaccttac gaaagtaac 
      aatcatgat aatggggcc tctgataac gatttagaa 
      acggtagat gacaccccg gaaaagtgt cacgaattt 
      atttgtcgg attgttgaa aatggggcc gataatggt 
      tttttatta agggggccc tacttagcc cgctttgag 
      ctttgttcg cgtctcgta aaatgtaac gttgactcg 
      agcgatgtt ctcggcgaa acaatcgaa ttatttatt 
      gagtatttt cgtaaattc ggacacaaa ccgtgctgt 
      gtttccgat ttacgccat tatttagat ttactagat 
      tgtgaaaaa aaggccgaa ttatcggca cgtttaatc 
      aaggatgtt gggatcagt tcaacaagt gtgcctcag 
      tcggaacaa caaatgcaa cgccatcta tgtgccctc 
      caattgtca cgtttatgt ggagcacat cgtaatcta 
      acttctgat catttgaaa gctttagtt actgcattt 
      tcgctacat tacgagcac ggttaccaa acttacggc 
      actaattta ctgtcgact gatttaggg ccttctgac 
      ccgtatgca cttttagcg gcgcacgtt ttgtacgat 
      ttatcacaa agtgagaaa aaatccgaa ccgattgtt 
      gccgctctt gtcttatta gaaaatatg ctcaagaat 
      agtccgagt aattttcac gcaaagttg ttgtgtgtg 
      cggctgtat catacagtg ggcggttgt ctcggtgcc 
      caaaacatg tacagtagt ttagacacg aaacattta 
      caattagat tcgttaggt tttatacat tgtgctaga 
      ttagcaacc accggtttg tattcgctg tgttcgaat 
      ttgtttgac gttacgtta aaattcttc tcgtcgaat 
      tacaaagat agttcggat catataaca ttttcgtat 
      aaatacggt tcgttcata aaactggac gaatttatg 
      gattttagg gaacgactg aacaacagc atgcactat 
      acaacagtt gcaatcgat cgaatagta ttgagttta 
      gttgaatgt acaacatta gacaccctg tacaacatc 
      gacatatca ccaaaagac gataaaatc gactgggag 
      gcgttacga gacaatcac gatcttagc gtttatatc 
      agttgggac cctgaacgt atcaaaggc ccgcctgaa 
      gaatacgaa gaaattcgc acatttttc aagcaaaat 
      aaaattttc cttaaatta cgtacgtca ctgctgtgg 
      gcgttatca gcagccgtg gacattgtc aaatcatca 
      gacggcgtc cgaaccaaa tacatggac caattggct 
      acgattttg gccgattgg gaggagcta agcaccaaa 
      aatcgaaac cacgcccct ttgtgtcaa cacctggtc 
      actttgccc ctcccctcg cgccttcac gtggccttg 
      gccacgccc cattttgac atcgtgggg cgaatgttg 
      cgcctttta ttggccgtt gtgtccgag ggttgttcc 
      aaagaagat gatttagtg gaaaaaatt gaaagtggg 
      gtcaaggcc atggtcgat atgattgaa aatcggggg 
      aagaactcg gctgatttg atttatcgg cgggaaacg 
      atggaatat gttgtgaat gcagtcgag gttttgtcc 
      gtttcgtcg ctcatttgt ttgttgtgc aatgatttg 
      gtgaagccg ccgagtaaa aaaggcaag aaaaaagtg 
      agcgaagcg tcgtcgtgg aaggaaacc gtggttcgg 
      ctggcaaat agtttcaaa acgcacatt aacgccttg 
      aatgaggtt ttgcaaact tgggcgcag tttagtgtt 
      tcgagtgat ctggcgctg atgctggag tcggtgaat 
      ttgagcaat gacggacac aatgtgtgc gagaatata 
      gtaaacagt tacgtgttg gccgttcga gagatacag 
      tgtgttttg aaaaataag attaaaatg ttgagtagt 
      atacagtaa
    mRNA sequence
    • >TC015420 RA
      gcgcctcga aaaatccgt aataaaata ccacttccg 
      gtggctgtt tttttccca aaatgtcaa ggaacatcc 
      ggtttgttc aaatttagc gcccacatt ttgacaatt 
      gacaattga cagaagccg acaaataaa tcaatcatg 
      gcggtttga agattagct caattcttg aagaaaaaa 
      aattatttt cgttcgatt tacacgtgt gtttggggt 
      aaaacagca caagagttg tctcaagta gcgtatttt 
      gcaaacacg agtcacaag ctaggttag gaagcgaaa 
      tgacttcga aggtccaac acgtgcagg acaactcag 
      tagccgaac ggagattgc gccccattt acgattggc 
      tggacatcg gcaacaaca aaaaagcgc tccaggagt 
      gcgagaaag tgctgaaaa agacgccga atttgctgt 
      gcgcgaaag cgctcaaag ccctgacgc tgcaccgga 
      tgggcaagg agggcgaag ccctggcca tcctggagg 
      ccctgactg ccgacagcc ccacggacg acgccacgc 
      tccaagcca tgactctgt gctacagag agttgcaac 
      aacttgaaa aaatttgta aattgtacg agactgcgg 
      tcaagttgg agccggcaa atgaggagc tttacacgc 
      atttattta tgtcttatg cgcgtgttt tcgatttta 
      aatcgcaac agaaaacgg caatgacgc tgtacaagc 
      acaagccca aaacgccgt actattgct gggctgtta 
      tgagtatta ttttacaag ccacgcgtg gcgagggca 
      agaacgatg cgaaaaaac gcagtttat tgctgagtc 
      tggccgaac gtatgatgg aaaagttga ttgtcgatg 
      ggaaacttg atgcggaac aggaagttc aactgtatg 
      ttatggttt tggagctgc agggcaagt ttgaggagg 
      ttttggtta ttttggatg ggcctctcg gtaccaagc 
      tggcttgta gtaatcagc cccataata agctgaagt 
      atttggtgc ccctcaagt gctgggatg atgttaatt 
      tgttgtgta aggggatct tgattgaga gtgtagaca 
      ggtgggata tttggaagg aatacatca actcggtgt 
      ttgccctaa tggaggcca aaccttacg aaagtaaca 
      atcatgata atggggcct ctgataacg atttagaaa 
      cggtagatg acaccccgg aaaagtgtc acgaattta 
      tttgtcgga ttgttgaaa atggggccg ataatggtt 
      ttttattaa gggggccct acttagccc gctttgagc 
      tttgttcgc gtctcgtaa aatgtaacg ttgactcga 
      gcgatgttc tcggcgaaa caatcgaat tatttattg 
      agtattttc gtaaattcg gacacaaac cgtgctgtg 
      tttccgatt tacgccatt atttagatt tactagatt 
      gtgaaaaaa aggccgaat tatcggcac gtttaatca 
      aggatgttg ggatcagtt caacaagtg tgcctcagt 
      cggaacaac aaatgcaac gccatctat gtgccctcc 
      aattgtcac gtttatgtg gagcacatc gtaatctaa 
      cttctgatc atttgaaag ctttagtta ctgcatttt 
      cgctacatt acgagcacg gttaccaaa cttacggca 
      ctaatttac tgtcgactg atttagggc cttctgacc 
      cgtatgcac ttttagcgg cgcacgttt tgtacgatt 
      tatcacaaa gtgagaaaa aatccgaac cgattgttg 
      ccgctcttg tcttattag aaaatatgc tcaagaata 
      gtccgagta attttcacg caaagttgt tgtgtgtgc 
      ggctgtatc atacagtgg gcggttgtc tcggtgccc 
      aaaacatgt acagtagtt tagacacga aacatttac 
      aattagatt cgttaggtt ttatacatt gtgctagat 
      tagcaacca ccggtttgt attcgctgt gttcgaatt 
      tgtttgacg ttacgttaa aattcttct cgtcgaatt 
      acaaagata gttcggatc atataacat tttcgtata 
      aatacggtt cgttcataa aactggacg aatttatgg 
      attttaggg aacgactga acaacagca tgcactata 
      caacagttg caatcgatc gaatagtat tgagtttag 
      ttgaatgta caacattag acaccctgt acaacatcg 
      acatatcac caaaagacg ataaaatcg actgggagg 
      cgttacgag acaatcacg atcttagcg tttatatca 
      gttgggacc ctgaacgta tcaaaggcc cgcctgaag 
      aatacgaag aaattcgca catttttca agcaaaata 
      aaattttcc ttaaattac gtacgtcac tgctgtggg 
      cgttatcag cagccgtgg acattgtca aatcatcag 
      acggcgtcc gaaccaaat acatggacc aattggcta 
      cgattttgg ccgattggg aggagctaa gcaccaaaa 
      atcgaaacc acgcccctt tgtgtcaac acctggtca 
      ctttgcccc tcccctcgc gccttcacg tggccttgg 
      ccacgcccc attttgaca tcgtggggc gaatgttgc 
      gccttttat tggccgttg tgtccgagg gttgttcca 
      aagaagatg atttagtgg aaaaaattg aaagtgggg 
      tcaaggcca tggtcgata tgattgaaa atcggggga 
      agaactcgg ctgatttga tttatcggc gggaaacga 
      tggaatatg ttgtgaatg cagtcgagg ttttgtccg 
      tttcgtcgc tcatttgtt tgttgtgca atgatttgg 
      tgaagccgc cgagtaaaa aaggcaaga aaaaagtga 
      gcgaagcgt cgtcgtgga aggaaaccg tggttcggc 
      tggcaaata gtttcaaaa cgcacatta acgccttga 
      atgaggttt tgcaaactt gggcgcagt ttagtgttt 
      cgagtgatc tggcgctga tgctggagt cggtgaatt 
      tgagcaatg acggacaca atgtgtgcg agaatatag 
      taaacagtt acgtgttgg ccgttcgag agatacagt 
      gtgttttga aaaataaga ttaaaatgt tgagtagta 
      tacagtaag gtgccattg caagagtga cacgtgtat 
      cagattggt tgaataaaa tggtttcta cgaaacacc 
      cacaacacg tcactagat ctttttttt taattgttt 
      tattccttg gtttttgcg aaaaatggt gagtttttg 
      gctggaaat tttcagaaa taaatgatt ttctagctg 
      ttggaaacg aagagagta gatagttcg tgagtcggg 
      aagttggac gtattttta tctgaattt ttggacgca 
      cttccgtta gaaaacaga ctgcacgtg agtgcaata 
      tttgccttt ttttattga ttgtcgtta ttttgcaaa 
      attcggacg attatttta ctaaaaatt cgctctttt 
      tgattaaaa cgcgttgaa aagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY