Status
Please wait ...
Gene information
General information
TC015410 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 526846..533042
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015410
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09144

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
    yolk late potentiallydecreased - Number of animals showing the phenotype: 4

    follicular epithelium bilateral potentially amount increased - Number of animals showing the phenotype: 2

    follicular epithelium central potentially amount decreased - Number of animals showing the phenotype: 2
    vitellogenic egg chamber sometimes fused - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015410 RA
      MAESGQNQA GADVPEDLF RGVKFFVSG EIAEKVVDL 
      LKVGGAERL NYFSDYVTH LICGENAEE NDITDATDL 
      YEIPAVTSK WVFASARLK KLVNTKPYV YNLNKLFTN 
      LVFCLSKVG SDREALWAV ITYHGGLVQ LNLTNKCTH 
      LVTVDTNSP KYEKAANLG ADKITVITP DWVVESVKN 
      NALAQADLF HPKLIQWPK VAKHESTIA ITGFEPEKV 
      ESVEKTNDM VTDSTQALL DKLKQRMPW IQPQTTTTA 
      DIVPPNVVA PSFLNKNQP NQPVRTFPQ QQSPQPAVV 
      QTNQQIPRS IVHHTPQPQ QLVQQQQPP PQQFNVPNQ 
      VHLNRLIGQ QQQQQQRPT LQQLRGQLA PNQLHQHLL 
      QQRIAQQNQ QNAQRPLTI QQQQLLQQQ IAQRQLLQN 
      QQQQQPQPQ TQFSNVIQQ QHILQQQKI FVSQQPNLA 
      VQQQFNQNQ QPQQFNQQF VQNQPDLNQ PQLVQQPAQ 
      FQPNQQLLL NQQTQFNQQ QQQAAQPQQ QRPQINQHL 
      WQQQQQMTI RHPVNVVQG PRIQWSPTQ QPNQPQRQF 
      IHLDAQTHN ELQKMNPEQ QAMFVQRLK QKQLLMQKQ 
      LTQRAGGGH ILIRGSVPP GLNPQQQMQ WLQRQAKQQ 
      GVVLQQQTI TQPNIQQQH APGLSPIQQ QQQQQQQQQ 
      PQQFDQNQQ QQLQLRQYR LQQLQLQRE QAQKNQIAA 
      VQQQPVQAG VVRPLGPVI PDSTTNPPE ANSQQLVVN 
      AKTKTALAN MLSNRLQSG GGTVPVPET IPEPSAAGT 
      LRLMTAQHN AALNPNNRP QDLIALQQQ RRVINGPNG 
      EIIRALVPQ SGVPGPVIA QTEPPKVQF SPRPAVPMQ 
      HRPGPFYGH NPNLKLPPD LFLLGCIFV VVEIERYLD 
      ESLPGWKQK IEKHGGEVE KQYCNRVTH VLCETQRHG 
      VVMQALRDC KRCITIYWL SDVMMRKQV LPPWTALHL 
      PTIYLDTTP AAKHLITLS GFTGCERNR VKHMINYTG 
      AKFTSYFSK HNTLLIAAK GEGAKYNHA KKWGIPVVN 
      VQWLTDIML GNFSALNQM EHIMYQQFP TPPNFSFDP 
      KLVPNLMHA WKMPINISQ ESYERVKRS SSPVLMPKK 
      AKKMKTEQT DENDNVDEG VEVNANYRI LFSMSSETA 
      TLQKIVKQL GGIVAKSHH DCTHLVMPS LSRTNKLLF 
      CICREVFIM PEQWLRDSH TAQKFLDEA SYSLDTKEF 
      NSEYKCDFT QTLNTRNRS KLFEGKFFW VTPSVFPSK 
      KVLVELVQS CGGIVEKIR RTSAQIEAT NINSPYSYI 
      IITHENDIH LVADLLRNK KDKVRIVCS AELIFSAIL 
      KQTFEVEPF AVKVL
    CDS sequence
    • >TC015410 RA
      atggccgag tctgggcaa aatcaagct ggggcggac 
      gtccccgag gacctgttc cgcggcgtg aagttcttc 
      gtcagtggg gaaattgct gaaaaggtg gttgatttg 
      ctgaaagtt ggtggggcc gagcgtttg aactatttc 
      tccgattat gtaacgcat ttaatttgc ggcgaaaac 
      gccgaggaa aatgatatc acggacgcc actgatctg 
      tacgaaatt ccggcggtt acgtccaag tgggttttt 
      gcgagcgcc cgtctgaag aagttagtt aataccaaa 
      ccgtacgtt tacaatttg aataagttg ttcacgaat 
      ttggtgttt tgcttatcg aaagttgga agcgatcgt 
      gaggcgctt tgggctgtc atcacttat catgggggc 
      cttgtccag ctcaatttg accaacaaa tgcacacat 
      ttagtcaca gtcgacaca aattcaccg aaatatgag 
      aaagccgcc aacttgggg gccgacaaa atcaccgta 
      ataacgccc gactgggtc gtagaatcg gtgaaaaac 
      aacgcattg gcacaggcg gacttgttc cacccgaag 
      ctgattcag tggccgaaa gttgccaaa catgaaagc 
      acaatcgcg atcacgggc ttcgagcct gaaaaagtt 
      gaatcagtt gagaaaaca aacgacatg gtgactgat 
      tcaacccag gctttatta gacaaactt aagcaaaga 
      atgccttgg attcaacct caaacaaca acaactgcc 
      gatattgtg ccacccaac gtggttgcg ccgagtttt 
      ctcaacaaa aatcaacca aatcaaccg gtgcgtact 
      ttcccgcaa caacaatca ccacagcct gctgttgta 
      caaacgaat caacaaatc ccccgaagt attgttcac 
      cacacgcct caaccgcaa caactcgtt caacaacaa 
      caaccacca ccacaacaa tttaatgtt ccaaatcag 
      gtccatttg aaccgactg atcggccaa caacaacaa 
      cagcaacaa cgcccgact cttcaacag ttgagaggt 
      cagttggcc ccgaatcaa ctgcaccaa cacttgtta 
      caacaacgt atcgcccaa caaaatcaa caaaatgcc 
      caacggccg ctcaccatt caacagcaa caattgttg 
      caacagcaa attgcccaa agacagtta ctacaaaat 
      caacagcaa caacaaccc caaccccag acgcagttt 
      agtaatgtt atccaacaa caacacatt ttacaacaa 
      cagaaaatt ttcgtatcg caacaacca aacctggcc 
      gtgcaacaa caattcaac cagaatcaa caaccgcaa 
      cagtttaat caacagttc gtacagaac caacccgac 
      ttaaatcaa ccgcaactg gtgcaacaa ccggcacaa 
      ttccaaccg aatcaacag ttgttgttg aatcaacaa 
      acccagttc aaccaacaa caacaacaa gcggcgcag 
      cctcagcaa caacgcccg caaatcaat caacacttg 
      tggcaacaa cagcaacaa atgactatc cgacatccg 
      gtcaatgtt gttcaaggc ccacgaatc caatggtcg 
      cccacccaa caacccaat caaccgcaa cgtcaattc 
      atacacttg gacgcccaa actcacaac gagctacaa 
      aaaatgaat cctgaacag caggccatg ttcgtgcaa 
      cgcctcaaa caaaaacaa ctcctaatg caaaaacaa 
      ttgacacag cgggccggt ggtgggcat attttgatc 
      cgtggttcg gtgcctcca ggtctcaac ccgcaacag 
      caaatgcag tggttgcaa aggcaagcg aaacaacaa 
      ggtgttgta ctgcaacaa caaaccatc actcagccc 
      aatattcaa caacaacac gcgccgggt ttgagtcca 
      attcaacaa cagcagcag cagcagcaa caacagcag 
      ccacaacag tttgaccag aatcaacaa caacagttg 
      cagttgagg cagtatagg ttacagcag ttgcaacta 
      cagcgtgaa caagcgcag aaaaatcaa atcgccgct 
      gtgcagcaa caaccagta caagcgggt gttgttagg 
      cctttagga ccggtgatt cccgattcg acgacgaat 
      ccacccgaa gccaactcg caacagttg gttgttaat 
      gccaaaact aagacggcc ttggcgaat atgttgagt 
      aatcggctg cagagtggg ggagggacg gtgcccgtt 
      ccggaaacg ataccggaa ccgtcagcg gcgggaacg 
      ctaaggttg atgacggct caacataac gccgctctt 
      aatccgaat aaccggccc caagattta attgccttg 
      caacaacaa cgtagagtt ataaatggc cctaatggc 
      gagattata agggcactg gtgccccaa tcaggagta 
      ccaggaccg gttatagcg caaactgag ccccctaaa 
      gtgcagttt tcgccccga ccggctgta ccgatgcaa 
      cataggccg gggccgttc tacggacac aatcccaac 
      ttaaaactc ccgccagat ttgtttctt ctggggtgc 
      atttttgtc gttgttgaa atcgagcgt tatttggac 
      gaatcattg cccggatgg aaacaaaaa attgaaaaa 
      catggaggc gaggttgaa aaacaatac tgtaatcgt 
      gtgacgcac gttttatgc gaaacgcaa cgtcacggc 
      gtcgtaatg caagcgtta cgcgactgt aaacgctgc 
      ataaccatt tattggtta agtgacgtc atgatgcgt 
      aaacaagtg ctgccacct tggaccgct ctccaccta 
      ccaactatt tacctcgac acaactcca gcagctaaa 
      cacttaata actttaagc ggatttaca gggtgtgag 
      cgaaatcgg gtcaaacat atgattaat tacacgggt 
      gctaagttt acgtcatac ttctccaaa cacaatact 
      ttattaatt gcggcgaaa ggcgagggc gcgaaatac 
      aatcacgcc aagaaatgg gggattccc gtggtgaat 
      gtccaatgg ctgactgac ataatgcta gggaatttc 
      tcggccttg aaccagatg gaacatata atgtaccaa 
      cagtttccc acgccgcct aatttcagc ttcgacccc 
      aaactagta ccgaatttg atgcatgcg tggaaaatg 
      ccaataaat atatcccaa gagtcgtac gaacgcgtt 
      aagcgaagc tcatcgccg gtcctaatg cccaaaaag 
      gcgaaaaaa atgaaaacc gagcaaacc gacgaaaac 
      gacaacgtg gacgaaggc gttgaagtg aacgcaaat 
      tacaggatc ctgttttcc atgtcttcc gaaacggcg 
      acgttgcaa aaaatcgtc aagcaactg ggcggcatt 
      gttgcgaaa tcgcaccac gactgcaca catctcgtg 
      atgccatct ttgagccgt actaataaa ctgttgttc 
      tgtatatgt cgcgaggtg tttataatg ccggaacag 
      tggcttagg gactcgcac acggcgcag aaattccta 
      gacgaggct agctatagt ctagacacc aaagaattc 
      aacagtgaa tataagtgc gactttacg caaactttg 
      aacacgagg aatcgcagc aagctgttc gaggggaaa 
      tttttctgg gtaacgccg agcgttttc ccgagtaaa 
      aaagtccta gttgagcta gtacagagt tgtgggggg 
      attgtcgaa aaaatacga cggacttcg gcgcaaatc 
      gaagcaaca aacattaat tcgccctac agttatatc 
      ataataacg cacgagaat gacattcac ttagttgcc 
      gatttgttg cggaataag aaagacaag gtgcggatt 
      gtttgcagt gccgagctg atcttcagt gccattttg 
      aaacaaact ttcgaagtc gagccgttc gctgtcaaa 
      gttttatga
    mRNA sequence
    • >TC015410 RA
      ctaaaagac acttccatg gccgtttaa tccggaaat 
      tttgggttt ggtgaattg aaacggtgc cctgtggct 
      aacttcaag tggctggat ccaacatgg agccggtgt 
      aaacaaaat atttttcct acaatttca aataatcca 
      aaaatgaaa taaagcccg caatggccg agtctgggc 
      aaaatcaag ctggggcgg acgtccccg aggacctgt 
      tccgcggcg tgaagttct tcgtcagtg gggaaattg 
      ctgaaaagg tggttgatt tgctgaaag ttggtgggg 
      ccgagcgtt tgaactatt tctccgatt atgtaacgc 
      atttaattt gcggcgaaa acgccgagg aaaatgata 
      tcacggacg ccactgatc tgtacgaaa ttccggcgg 
      ttacgtcca agtgggttt ttgcgagcg cccgtctga 
      agaagttag ttaatacca aaccgtacg tttacaatt 
      tgaataagt tgttcacga atttggtgt tttgcttat 
      cgaaagttg gaagcgatc gtgaggcgc tttgggctg 
      tcatcactt atcatgggg gccttgtcc agctcaatt 
      tgaccaaca aatgcacac atttagtca cagtcgaca 
      caaattcac cgaaatatg agaaagccg ccaacttgg 
      gggccgaca aaatcaccg taataacgc ccgactggg 
      tcgtagaat cggtgaaaa acaacgcat tggcacagg 
      cggacttgt tccacccga agctgattc agtggccga 
      aagttgcca aacatgaaa gcacaatcg cgatcacgg 
      gcttcgagc ctgaaaaag ttgaatcag ttgagaaaa 
      caaacgaca tggtgactg attcaaccc aggctttat 
      tagacaaac ttaagcaaa gaatgcctt ggattcaac 
      ctcaaacaa caacaactg ccgatattg tgccaccca 
      acgtggttg cgccgagtt ttctcaaca aaaatcaac 
      caaatcaac cggtgcgta ctttcccgc aacaacaat 
      caccacagc ctgctgttg tacaaacga atcaacaaa 
      tcccccgaa gtattgttc accacacgc ctcaaccgc 
      aacaactcg ttcaacaac aacaaccac caccacaac 
      aatttaatg ttccaaatc aggtccatt tgaaccgac 
      tgatcggcc aacaacaac aacagcaac aacgcccga 
      ctcttcaac agttgagag gtcagttgg ccccgaatc 
      aactgcacc aacacttgt tacaacaac gtatcgccc 
      aacaaaatc aacaaaatg cccaacggc cgctcacca 
      ttcaacagc aacaattgt tgcaacagc aaattgccc 
      aaagacagt tactacaaa atcaacagc aacaacaac 
      cccaacccc agacgcagt ttagtaatg ttatccaac 
      aacaacaca ttttacaac aacagaaaa ttttcgtat 
      cgcaacaac caaacctgg ccgtgcaac aacaattca 
      accagaatc aacaaccgc aacagttta atcaacagt 
      tcgtacaga accaacccg acttaaatc aaccgcaac 
      tggtgcaac aaccggcac aattccaac cgaatcaac 
      agttgttgt tgaatcaac aaacccagt tcaaccaac 
      aacaacaac aagcggcgc agcctcagc aacaacgcc 
      cgcaaatca atcaacact tgtggcaac aacagcaac 
      aaatgacta tccgacatc cggtcaatg ttgttcaag 
      gcccacgaa tccaatggt cgcccaccc aacaaccca 
      atcaaccgc aacgtcaat tcatacact tggacgccc 
      aaactcaca acgagctac aaaaaatga atcctgaac 
      agcaggcca tgttcgtgc aacgcctca aacaaaaac 
      aactcctaa tgcaaaaac aattgacac agcgggccg 
      gtggtgggc atattttga tccgtggtt cggtgcctc 
      caggtctca acccgcaac agcaaatgc agtggttgc 
      aaaggcaag cgaaacaac aaggtgttg tactgcaac 
      aacaaacca tcactcagc ccaatattc aacaacaac 
      acgcgccgg gtttgagtc caattcaac aacagcagc 
      agcagcagc aacaacagc agccacaac agtttgacc 
      agaatcaac aacaacagt tgcagttga ggcagtata 
      ggttacagc agttgcaac tacagcgtg aacaagcgc 
      agaaaaatc aaatcgccg ctgtgcagc aacaaccag 
      tacaagcgg gtgttgtta ggcctttag gaccggtga 
      ttcccgatt cgacgacga atccacccg aagccaact 
      cgcaacagt tggttgtta atgccaaaa ctaagacgg 
      ccttggcga atatgttga gtaatcggc tgcagagtg 
      ggggaggga cggtgcccg ttccggaaa cgataccgg 
      aaccgtcag cggcgggaa cgctaaggt tgatgacgg 
      ctcaacata acgccgctc ttaatccga ataaccggc 
      cccaagatt taattgcct tgcaacaac aacgtagag 
      ttataaatg gccctaatg gcgagatta taagggcac 
      tggtgcccc aatcaggag taccaggac cggttatag 
      cgcaaactg agcccccta aagtgcagt tttcgcccc 
      gaccggctg taccgatgc aacataggc cggggccgt 
      tctacggac acaatccca acttaaaac tcccgccag 
      atttgtttc ttctggggt gcatttttg tcgttgttg 
      aaatcgagc gttatttgg acgaatcat tgcccggat 
      ggaaacaaa aaattgaaa aacatggag gcgaggttg 
      aaaaacaat actgtaatc gtgtgacgc acgttttat 
      gcgaaacgc aacgtcacg gcgtcgtaa tgcaagcgt 
      tacgcgact gtaaacgct gcataacca tttattggt 
      taagtgacg tcatgatgc gtaaacaag tgctgccac 
      cttggaccg ctctccacc taccaacta tttacctcg 
      acacaactc cagcagcta aacacttaa taactttaa 
      gcggattta cagggtgtg agcgaaatc gggtcaaac 
      atatgatta attacacgg gtgctaagt ttacgtcat 
      acttctcca aacacaata ctttattaa ttgcggcga 
      aaggcgagg gcgcgaaat acaatcacg ccaagaaat 
      gggggattc ccgtggtga atgtccaat ggctgactg 
      acataatgc tagggaatt tctcggcct tgaaccaga 
      tggaacata taatgtacc aacagtttc ccacgccgc 
      ctaatttca gcttcgacc ccaaactag taccgaatt 
      tgatgcatg cgtggaaaa tgccaataa atatatccc 
      aagagtcgt acgaacgcg ttaagcgaa gctcatcgc 
      cggtcctaa tgcccaaaa aggcgaaaa aaatgaaaa 
      ccgagcaaa ccgacgaaa acgacaacg tggacgaag 
      gcgttgaag tgaacgcaa attacagga tcctgtttt 
      ccatgtctt ccgaaacgg cgacgttgc aaaaaatcg 
      tcaagcaac tgggcggca ttgttgcga aatcgcacc 
      acgactgca cacatctcg tgatgccat ctttgagcc 
      gtactaata aactgttgt tctgtatat gtcgcgagg 
      tgtttataa tgccggaac agtggctta gggactcgc 
      acacggcgc agaaattcc tagacgagg ctagctata 
      gtctagaca ccaaagaat tcaacagtg aatataagt 
      gcgacttta cgcaaactt tgaacacga ggaatcgca 
      gcaagctgt tcgagggga aatttttct gggtaacgc 
      cgagcgttt tcccgagta aaaaagtcc tagttgagc 
      tagtacaga gttgtgggg ggattgtcg aaaaaatac 
      gacggactt cggcgcaaa tcgaagcaa caaacatta 
      attcgccct acagttata tcataataa cgcacgaga 
      atgacattc acttagttg ccgatttgt tgcggaata 
      agaaagaca aggtgcgga ttgtttgca gtgccgagc 
      tgatcttca gtgccattt tgaaacaaa ctttcgaag 
      tcgagccgt tcgctgtca aagttttat gaaattaag 
      tgtacatat ttttcgaca taggaccaa aaccgtgtt 
      tttagggtt acgttttgt taatttgtg atttctttg 
      tcagatttt gtaaaattt ctaagataa taaaagtta 
      cacaaattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY