| General information | |||
| TC015410 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 526846..533042 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015410 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_09144 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
yolk late potentiallydecreased - Number of animals showing the phenotype: 4
follicular epithelium bilateral potentially amount increased - Number of animals showing the phenotype: 2
follicular epithelium central potentially amount decreased - Number of animals showing the phenotype: 2
vitellogenic egg chamber sometimes fused - Number of animals showing the phenotype: 2
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015410 RA
MAESGQNQA GADVPEDLF RGVKFFVSG EIAEKVVDL LKVGGAERL NYFSDYVTH LICGENAEE NDITDATDL YEIPAVTSK WVFASARLK KLVNTKPYV YNLNKLFTN LVFCLSKVG SDREALWAV ITYHGGLVQ LNLTNKCTH LVTVDTNSP KYEKAANLG ADKITVITP DWVVESVKN NALAQADLF HPKLIQWPK VAKHESTIA ITGFEPEKV ESVEKTNDM VTDSTQALL DKLKQRMPW IQPQTTTTA DIVPPNVVA PSFLNKNQP NQPVRTFPQ QQSPQPAVV QTNQQIPRS IVHHTPQPQ QLVQQQQPP PQQFNVPNQ VHLNRLIGQ QQQQQQRPT LQQLRGQLA PNQLHQHLL QQRIAQQNQ QNAQRPLTI QQQQLLQQQ IAQRQLLQN QQQQQPQPQ TQFSNVIQQ QHILQQQKI FVSQQPNLA VQQQFNQNQ QPQQFNQQF VQNQPDLNQ PQLVQQPAQ FQPNQQLLL NQQTQFNQQ QQQAAQPQQ QRPQINQHL WQQQQQMTI RHPVNVVQG PRIQWSPTQ QPNQPQRQF IHLDAQTHN ELQKMNPEQ QAMFVQRLK QKQLLMQKQ LTQRAGGGH ILIRGSVPP GLNPQQQMQ WLQRQAKQQ GVVLQQQTI TQPNIQQQH APGLSPIQQ QQQQQQQQQ PQQFDQNQQ QQLQLRQYR LQQLQLQRE QAQKNQIAA VQQQPVQAG VVRPLGPVI PDSTTNPPE ANSQQLVVN AKTKTALAN MLSNRLQSG GGTVPVPET IPEPSAAGT LRLMTAQHN AALNPNNRP QDLIALQQQ RRVINGPNG EIIRALVPQ SGVPGPVIA QTEPPKVQF SPRPAVPMQ HRPGPFYGH NPNLKLPPD LFLLGCIFV VVEIERYLD ESLPGWKQK IEKHGGEVE KQYCNRVTH VLCETQRHG VVMQALRDC KRCITIYWL SDVMMRKQV LPPWTALHL PTIYLDTTP AAKHLITLS GFTGCERNR VKHMINYTG AKFTSYFSK HNTLLIAAK GEGAKYNHA KKWGIPVVN VQWLTDIML GNFSALNQM EHIMYQQFP TPPNFSFDP KLVPNLMHA WKMPINISQ ESYERVKRS SSPVLMPKK AKKMKTEQT DENDNVDEG VEVNANYRI LFSMSSETA TLQKIVKQL GGIVAKSHH DCTHLVMPS LSRTNKLLF CICREVFIM PEQWLRDSH TAQKFLDEA SYSLDTKEF NSEYKCDFT QTLNTRNRS KLFEGKFFW VTPSVFPSK KVLVELVQS CGGIVEKIR RTSAQIEAT NINSPYSYI IITHENDIH LVADLLRNK KDKVRIVCS AELIFSAIL KQTFEVEPF AVKVL
- >TC015410 RA
atggccgag tctgggcaa aatcaagct ggggcggac gtccccgag gacctgttc cgcggcgtg aagttcttc gtcagtggg gaaattgct gaaaaggtg gttgatttg ctgaaagtt ggtggggcc gagcgtttg aactatttc tccgattat gtaacgcat ttaatttgc ggcgaaaac gccgaggaa aatgatatc acggacgcc actgatctg tacgaaatt ccggcggtt acgtccaag tgggttttt gcgagcgcc cgtctgaag aagttagtt aataccaaa ccgtacgtt tacaatttg aataagttg ttcacgaat ttggtgttt tgcttatcg aaagttgga agcgatcgt gaggcgctt tgggctgtc atcacttat catgggggc cttgtccag ctcaatttg accaacaaa tgcacacat ttagtcaca gtcgacaca aattcaccg aaatatgag aaagccgcc aacttgggg gccgacaaa atcaccgta ataacgccc gactgggtc gtagaatcg gtgaaaaac aacgcattg gcacaggcg gacttgttc cacccgaag ctgattcag tggccgaaa gttgccaaa catgaaagc acaatcgcg atcacgggc ttcgagcct gaaaaagtt gaatcagtt gagaaaaca aacgacatg gtgactgat tcaacccag gctttatta gacaaactt aagcaaaga atgccttgg attcaacct caaacaaca acaactgcc gatattgtg ccacccaac gtggttgcg ccgagtttt ctcaacaaa aatcaacca aatcaaccg gtgcgtact ttcccgcaa caacaatca ccacagcct gctgttgta caaacgaat caacaaatc ccccgaagt attgttcac cacacgcct caaccgcaa caactcgtt caacaacaa caaccacca ccacaacaa tttaatgtt ccaaatcag gtccatttg aaccgactg atcggccaa caacaacaa cagcaacaa cgcccgact cttcaacag ttgagaggt cagttggcc ccgaatcaa ctgcaccaa cacttgtta caacaacgt atcgcccaa caaaatcaa caaaatgcc caacggccg ctcaccatt caacagcaa caattgttg caacagcaa attgcccaa agacagtta ctacaaaat caacagcaa caacaaccc caaccccag acgcagttt agtaatgtt atccaacaa caacacatt ttacaacaa cagaaaatt ttcgtatcg caacaacca aacctggcc gtgcaacaa caattcaac cagaatcaa caaccgcaa cagtttaat caacagttc gtacagaac caacccgac ttaaatcaa ccgcaactg gtgcaacaa ccggcacaa ttccaaccg aatcaacag ttgttgttg aatcaacaa acccagttc aaccaacaa caacaacaa gcggcgcag cctcagcaa caacgcccg caaatcaat caacacttg tggcaacaa cagcaacaa atgactatc cgacatccg gtcaatgtt gttcaaggc ccacgaatc caatggtcg cccacccaa caacccaat caaccgcaa cgtcaattc atacacttg gacgcccaa actcacaac gagctacaa aaaatgaat cctgaacag caggccatg ttcgtgcaa cgcctcaaa caaaaacaa ctcctaatg caaaaacaa ttgacacag cgggccggt ggtgggcat attttgatc cgtggttcg gtgcctcca ggtctcaac ccgcaacag caaatgcag tggttgcaa aggcaagcg aaacaacaa ggtgttgta ctgcaacaa caaaccatc actcagccc aatattcaa caacaacac gcgccgggt ttgagtcca attcaacaa cagcagcag cagcagcaa caacagcag ccacaacag tttgaccag aatcaacaa caacagttg cagttgagg cagtatagg ttacagcag ttgcaacta cagcgtgaa caagcgcag aaaaatcaa atcgccgct gtgcagcaa caaccagta caagcgggt gttgttagg cctttagga ccggtgatt cccgattcg acgacgaat ccacccgaa gccaactcg caacagttg gttgttaat gccaaaact aagacggcc ttggcgaat atgttgagt aatcggctg cagagtggg ggagggacg gtgcccgtt ccggaaacg ataccggaa ccgtcagcg gcgggaacg ctaaggttg atgacggct caacataac gccgctctt aatccgaat aaccggccc caagattta attgccttg caacaacaa cgtagagtt ataaatggc cctaatggc gagattata agggcactg gtgccccaa tcaggagta ccaggaccg gttatagcg caaactgag ccccctaaa gtgcagttt tcgccccga ccggctgta ccgatgcaa cataggccg gggccgttc tacggacac aatcccaac ttaaaactc ccgccagat ttgtttctt ctggggtgc atttttgtc gttgttgaa atcgagcgt tatttggac gaatcattg cccggatgg aaacaaaaa attgaaaaa catggaggc gaggttgaa aaacaatac tgtaatcgt gtgacgcac gttttatgc gaaacgcaa cgtcacggc gtcgtaatg caagcgtta cgcgactgt aaacgctgc ataaccatt tattggtta agtgacgtc atgatgcgt aaacaagtg ctgccacct tggaccgct ctccaccta ccaactatt tacctcgac acaactcca gcagctaaa cacttaata actttaagc ggatttaca gggtgtgag cgaaatcgg gtcaaacat atgattaat tacacgggt gctaagttt acgtcatac ttctccaaa cacaatact ttattaatt gcggcgaaa ggcgagggc gcgaaatac aatcacgcc aagaaatgg gggattccc gtggtgaat gtccaatgg ctgactgac ataatgcta gggaatttc tcggccttg aaccagatg gaacatata atgtaccaa cagtttccc acgccgcct aatttcagc ttcgacccc aaactagta ccgaatttg atgcatgcg tggaaaatg ccaataaat atatcccaa gagtcgtac gaacgcgtt aagcgaagc tcatcgccg gtcctaatg cccaaaaag gcgaaaaaa atgaaaacc gagcaaacc gacgaaaac gacaacgtg gacgaaggc gttgaagtg aacgcaaat tacaggatc ctgttttcc atgtcttcc gaaacggcg acgttgcaa aaaatcgtc aagcaactg ggcggcatt gttgcgaaa tcgcaccac gactgcaca catctcgtg atgccatct ttgagccgt actaataaa ctgttgttc tgtatatgt cgcgaggtg tttataatg ccggaacag tggcttagg gactcgcac acggcgcag aaattccta gacgaggct agctatagt ctagacacc aaagaattc aacagtgaa tataagtgc gactttacg caaactttg aacacgagg aatcgcagc aagctgttc gaggggaaa tttttctgg gtaacgccg agcgttttc ccgagtaaa aaagtccta gttgagcta gtacagagt tgtgggggg attgtcgaa aaaatacga cggacttcg gcgcaaatc gaagcaaca aacattaat tcgccctac agttatatc ataataacg cacgagaat gacattcac ttagttgcc gatttgttg cggaataag aaagacaag gtgcggatt gtttgcagt gccgagctg atcttcagt gccattttg aaacaaact ttcgaagtc gagccgttc gctgtcaaa gttttatga
- >TC015410 RA
ctaaaagac acttccatg gccgtttaa tccggaaat tttgggttt ggtgaattg aaacggtgc cctgtggct aacttcaag tggctggat ccaacatgg agccggtgt aaacaaaat atttttcct acaatttca aataatcca aaaatgaaa taaagcccg caatggccg agtctgggc aaaatcaag ctggggcgg acgtccccg aggacctgt tccgcggcg tgaagttct tcgtcagtg gggaaattg ctgaaaagg tggttgatt tgctgaaag ttggtgggg ccgagcgtt tgaactatt tctccgatt atgtaacgc atttaattt gcggcgaaa acgccgagg aaaatgata tcacggacg ccactgatc tgtacgaaa ttccggcgg ttacgtcca agtgggttt ttgcgagcg cccgtctga agaagttag ttaatacca aaccgtacg tttacaatt tgaataagt tgttcacga atttggtgt tttgcttat cgaaagttg gaagcgatc gtgaggcgc tttgggctg tcatcactt atcatgggg gccttgtcc agctcaatt tgaccaaca aatgcacac atttagtca cagtcgaca caaattcac cgaaatatg agaaagccg ccaacttgg gggccgaca aaatcaccg taataacgc ccgactggg tcgtagaat cggtgaaaa acaacgcat tggcacagg cggacttgt tccacccga agctgattc agtggccga aagttgcca aacatgaaa gcacaatcg cgatcacgg gcttcgagc ctgaaaaag ttgaatcag ttgagaaaa caaacgaca tggtgactg attcaaccc aggctttat tagacaaac ttaagcaaa gaatgcctt ggattcaac ctcaaacaa caacaactg ccgatattg tgccaccca acgtggttg cgccgagtt ttctcaaca aaaatcaac caaatcaac cggtgcgta ctttcccgc aacaacaat caccacagc ctgctgttg tacaaacga atcaacaaa tcccccgaa gtattgttc accacacgc ctcaaccgc aacaactcg ttcaacaac aacaaccac caccacaac aatttaatg ttccaaatc aggtccatt tgaaccgac tgatcggcc aacaacaac aacagcaac aacgcccga ctcttcaac agttgagag gtcagttgg ccccgaatc aactgcacc aacacttgt tacaacaac gtatcgccc aacaaaatc aacaaaatg cccaacggc cgctcacca ttcaacagc aacaattgt tgcaacagc aaattgccc aaagacagt tactacaaa atcaacagc aacaacaac cccaacccc agacgcagt ttagtaatg ttatccaac aacaacaca ttttacaac aacagaaaa ttttcgtat cgcaacaac caaacctgg ccgtgcaac aacaattca accagaatc aacaaccgc aacagttta atcaacagt tcgtacaga accaacccg acttaaatc aaccgcaac tggtgcaac aaccggcac aattccaac cgaatcaac agttgttgt tgaatcaac aaacccagt tcaaccaac aacaacaac aagcggcgc agcctcagc aacaacgcc cgcaaatca atcaacact tgtggcaac aacagcaac aaatgacta tccgacatc cggtcaatg ttgttcaag gcccacgaa tccaatggt cgcccaccc aacaaccca atcaaccgc aacgtcaat tcatacact tggacgccc aaactcaca acgagctac aaaaaatga atcctgaac agcaggcca tgttcgtgc aacgcctca aacaaaaac aactcctaa tgcaaaaac aattgacac agcgggccg gtggtgggc atattttga tccgtggtt cggtgcctc caggtctca acccgcaac agcaaatgc agtggttgc aaaggcaag cgaaacaac aaggtgttg tactgcaac aacaaacca tcactcagc ccaatattc aacaacaac acgcgccgg gtttgagtc caattcaac aacagcagc agcagcagc aacaacagc agccacaac agtttgacc agaatcaac aacaacagt tgcagttga ggcagtata ggttacagc agttgcaac tacagcgtg aacaagcgc agaaaaatc aaatcgccg ctgtgcagc aacaaccag tacaagcgg gtgttgtta ggcctttag gaccggtga ttcccgatt cgacgacga atccacccg aagccaact cgcaacagt tggttgtta atgccaaaa ctaagacgg ccttggcga atatgttga gtaatcggc tgcagagtg ggggaggga cggtgcccg ttccggaaa cgataccgg aaccgtcag cggcgggaa cgctaaggt tgatgacgg ctcaacata acgccgctc ttaatccga ataaccggc cccaagatt taattgcct tgcaacaac aacgtagag ttataaatg gccctaatg gcgagatta taagggcac tggtgcccc aatcaggag taccaggac cggttatag cgcaaactg agcccccta aagtgcagt tttcgcccc gaccggctg taccgatgc aacataggc cggggccgt tctacggac acaatccca acttaaaac tcccgccag atttgtttc ttctggggt gcatttttg tcgttgttg aaatcgagc gttatttgg acgaatcat tgcccggat ggaaacaaa aaattgaaa aacatggag gcgaggttg aaaaacaat actgtaatc gtgtgacgc acgttttat gcgaaacgc aacgtcacg gcgtcgtaa tgcaagcgt tacgcgact gtaaacgct gcataacca tttattggt taagtgacg tcatgatgc gtaaacaag tgctgccac cttggaccg ctctccacc taccaacta tttacctcg acacaactc cagcagcta aacacttaa taactttaa gcggattta cagggtgtg agcgaaatc gggtcaaac atatgatta attacacgg gtgctaagt ttacgtcat acttctcca aacacaata ctttattaa ttgcggcga aaggcgagg gcgcgaaat acaatcacg ccaagaaat gggggattc ccgtggtga atgtccaat ggctgactg acataatgc tagggaatt tctcggcct tgaaccaga tggaacata taatgtacc aacagtttc ccacgccgc ctaatttca gcttcgacc ccaaactag taccgaatt tgatgcatg cgtggaaaa tgccaataa atatatccc aagagtcgt acgaacgcg ttaagcgaa gctcatcgc cggtcctaa tgcccaaaa aggcgaaaa aaatgaaaa ccgagcaaa ccgacgaaa acgacaacg tggacgaag gcgttgaag tgaacgcaa attacagga tcctgtttt ccatgtctt ccgaaacgg cgacgttgc aaaaaatcg tcaagcaac tgggcggca ttgttgcga aatcgcacc acgactgca cacatctcg tgatgccat ctttgagcc gtactaata aactgttgt tctgtatat gtcgcgagg tgtttataa tgccggaac agtggctta gggactcgc acacggcgc agaaattcc tagacgagg ctagctata gtctagaca ccaaagaat tcaacagtg aatataagt gcgacttta cgcaaactt tgaacacga ggaatcgca gcaagctgt tcgagggga aatttttct gggtaacgc cgagcgttt tcccgagta aaaaagtcc tagttgagc tagtacaga gttgtgggg ggattgtcg aaaaaatac gacggactt cggcgcaaa tcgaagcaa caaacatta attcgccct acagttata tcataataa cgcacgaga atgacattc acttagttg ccgatttgt tgcggaata agaaagaca aggtgcgga ttgtttgca gtgccgagc tgatcttca gtgccattt tgaaacaaa ctttcgaag tcgagccgt tcgctgtca aagttttat gaaattaag tgtacatat ttttcgaca taggaccaa aaccgtgtt tttagggtt acgttttgt taatttgtg atttctttg tcagatttt gtaaaattt ctaagataa taaaagtta cacaaattg
