Status
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Gene information
General information
TC011290 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2257862..2279207
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011290
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09143

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011290 RA
      MGGKHKPAQ RIKNNARPS SSGRSAELL ASSSQVPQF 
      AAVKEIRFV PPTLSLLKT EEFDSSVSP AFQIVFKKM 
      NKKDCTTKL KALQEFCDL IASSEAEAV KTILAFWAT 
      LYSILATDT DHRVREAVH NAHHQIILK DKKMIAPFL 
      KQLIGPWFT SQYDNYPPA ATAASKAFN AAFPQNKVQ 
      DCVVFCQKE ILTYIYDNL TSKIESNPK HGTNEDAEA 
      KFERIIISS LQGYCLYLN TLTQEQIEK SEDLNKQII 
      SNSRFLKLA THKTAQIRA TWFKAVAIL YQKAPFLLE 
      EEHKNFVPV VFNNLDESE PPVVGSVWD AALLIMDLG 
      QEWWKLINI EKSYLSKLK KVLREGGQG NATVIYPNL 
      LSLLHKLPP SVRVDQFYD SFFENLRCG LKRKSVISS 
      RSESTAVVA ALVECLQYV ILVNQENCD LCVKLIKSH 
      LLPLIEWSL RAEQVCYRT VFHQIALLV QYWNRNKNL 
      ANYSEYLAL FWSNVVTLV ESVSKFEEE NEDLVDRQI 
      VFLQHLKTI AKPKEQLKV KFEEEARSE SHLEGATQI 
      DASYNENLT LLVYTLLKT YVVQIEENK SKVLIPNVY 
      AVVRLFESR EFLVGLSRS LYQSDNLVT VYDKLFEWL 
      KSDELSCET LVDLAFILL NYVNDDEKN VILESFLQL 
      SDRQLFGWC VKNAFSHPY NQDSSVQSW IKRSEVGRF 
      LVSVADCNL KIDCPPDLK VLFKLAFAE NEDGELLID 
      KNTLNQIIE KVRAPLLEP RSFEVAIDS CVHLASYIT 
      GTIYTENTL LKFGEDLLL ALFQFSCND HTDLEFLTD 
      DTEWEVNTA WQDVVMIFA KNLPKNELQ NLNGKFADL 
      VEENFLGKF PTDVNTNCL IQTIVSFIK AVLNGQPFM 
      ASEVLRLFF ERSFVPTWK NKIEDLCLQ AEYLTGSLS 
      RPENYKKET SQVSQEEIL KYFVWSFIK ISVLSSPLE 
      EDEEENAPK DIIYVTDDQ KQLIMDVLY DLTMCQSFL 
      TNYKITKHY KNLSQYYDF IKEKTTQTL EKIQIGDEM 
      TELFISQLN SNSGLWSKV IYLYNQEIF DKPPQETFK 
      TYHNQTTSD LSQLHLVQL YGPHLDFDH VATSSVLND 
      VVILRSLAH CDEIDVQIA EIFEKIKKL RNRDIPKFL 
      FENKERTCA ESEVVIEVI RLFTRLIKV KFENLSRQH 
      WDFGVISLA SWASNCCKF RSGYDSGKN QAFMVAISE 
      FYIALNEKI NELKRNNVK SGFVDEWEE LFLPNVHLD 
      LVQLWLYLA EQFASKTCL LDYHPFLQE FGKVTDHFV 
      YKLIFSKEN TLPSWSKIL KRCAVLLTN STSILQLWG 
      YKMLLILVP GLIEIDLQS DSSSTPNTK GFTIGQFKE 
      ILLQTHSIV DGVLAGFKL GEDSCQVTP FTDSYTYVF 
      AYLLLWGVL LSMCEKSTP ELRYQYADW FRNDDYFKS 
      LLNNLFKLM PTEILHYSE GKTKAFSKL FSQNPGLGF 
      KEPCTSEQL EHIVCWIYS FCLAQLPAL VRQWWSQTE 
      TKVTQIVEK VTAFYVSPQ LCNQELLDI TQHENKFKN 
      MTVKIVPSV REVIAVYTV DEAQMELVV TLPQNYPLG 
      DPDVRCSRQ IGGPLHKQW LMQLKKYLV HQNGRIWDG 
      LSLWNSSLD KKFDGVEEC YICFAVLHP GTYQLPKLT 
      CQTCKKKFH SACLYKWFS TSNKSSCPI CRNLF
    CDS sequence
    • >TC011290 RA
      atgggcggc aagcacaag cccgcccaa agaatcaag 
      aacaatgcg aggccttcc agcagtggg cgcagcgcc 
      gagttgctg gcgtcctcc tcacaagtg ccccaattc 
      gccgcagtt aaggagatc cggtttgtg cccccgacc 
      ctgtctttg ctcaaaacg gaggaattt gactcgtcc 
      gtgtcgcca gcgtttcag atcgtattc aagaaaatg 
      aacaaaaag gactgcacc actaaatta aaagccttg 
      caggagttt tgcgaccta atcgcgagt tccgaggcg 
      gaggctgtt aagacgatc ttggcgttt tgggccacg 
      ctgtatagc attttggcc accgacacg gatcatagg 
      gtgcgggag gcggtgcac aacgcccac catcaaatc 
      attttgaag gataaaaaa atgatagcc ccctttttg 
      aaacagttg atagggccc tggttcacc tcacagtat 
      gataattat ccgccggcg gccacggcc gcgtctaaa 
      gctttcaac gccgcgttt ccgcaaaac aaggtccag 
      gattgtgtg gtgttttgc caaaaggaa atcttgacg 
      tacatttac gataatttg acttcgaaa attgaatct 
      aatcctaaa catggcaca aatgaggat gctgaagcg 
      aaatttgag agaatcatt atttcgagt cttcaaggc 
      tactgcctc tatttgaat acactgact caggaacag 
      atcgagaaa agtgaggat ttgaacaag caaattata 
      agtaattca aggtttctt aaattggca acgcataaa 
      acggcacaa attcgggcc acttggttc aaagccgtt 
      gcgattttg taccaaaaa gccccattt ttgttagaa 
      gaggaacat aagaatttt gttccggtt gttttcaat 
      aatttggat gagagtgaa ccccctgtt gtgggttct 
      gtgtgggat gcggctttg ctcattatg gatttagga 
      caggaatgg tggaaactt atcaacatt gaaaaaagt 
      tacttatca aagttgaaa aaagtccta agggaagga 
      ggccaaggc aacgcgacc gttatctac cctaatcta 
      ttatcactt ttgcacaag ttgcccccg tctgtgcgt 
      gtggaccaa ttttatgac agttttttc gaaaattta 
      cgatgtgga ttgaagcgc aaaagtgtg ataagtagt 
      cgatccgag tcaacggct gtggtcgca gccttagta 
      gaatgtcta caatacgtg atattagtt aaccaagaa 
      aattgcgat ctgtgcgta aaattaata aaatcgcat 
      ttgttgcct ttgattgag tggagtttg agggcggaa 
      caagtctgt taccgcact gttttccac caaatcgcc 
      cttctagtg cagtactgg aacagaaat aaaaatcta 
      gccaattat tccgaatat ttggcgttg ttttggagc 
      aacgttgta actttagtc gagtcagtg tcgaagttt 
      gaggaggaa aatgaggat ttggtcgat cgacaaatc 
      gtatttttg caacattta aaaacaatt gccaaacca 
      aaagaacaa ttgaaagtg aaatttgaa gaagaagcg 
      aggagtgag tcgcaccta gagggcgcc acacaaatc 
      gatgcttcg tacaacgaa aatctcact ttgctagtc 
      tacactttg ctcaaaacg tacgttgtc caaattgaa 
      gaaaacaaa tcgaaagtt ttaatcccg aatgtttac 
      gctgtggtg cgtttgttc gaaagtcgg gaatttctc 
      gtcggtttg agcagaagt ctgtaccag tcggataat 
      ttggttact gtttatgat aaattgttt gagtggtta 
      aagtcggat gaattgtcc tgcgagact ttggtcgat 
      ttggcgttt attcttttg aattatgtt aacgatgat 
      gagaaaaat gtgattttg gagtcgttt ttgcaactg 
      tccgatcgt caattgttt ggctggtgt gtcaaaaat 
      gcgttctca cacccctac aaccaggac tccagtgtc 
      caaagttgg atcaagcgg tcggaagtt ggacgtttt 
      ttggtgtct gttgctgat tgtaattta aaaattgat 
      tgcccgccc gatttgaag gttttattc aagttggcg 
      ttcgccgaa aatgaggat ggagaatta ttaattgac 
      aaaaacact ttgaaccaa ataattgag aaagtgcga 
      gctccgctt cttgagccc cgcagtttt gaggtcgcg 
      attgattcc tgcgtccat ctagcgtcc tacattacc 
      ggcaccatc tacaccgaa aacaccctg ttaaagttc 
      ggagaagac ttactattg gcccttttc caattctcc 
      tgcaacgac cacacagat ttggagttt ttgactgac 
      gatacggaa tgggaggtg aatacggcg tggcaagac 
      gttgtaatg atttttgcg aagaatttg cccaaaaac 
      gaattgcaa aatttgaac ggaaaattc gccgatttg 
      gttgaggaa aatttcttg ggaaaattc ccaacagac 
      gtaaatacg aattgtttg atccaaact atagttagt 
      ttcatcaag gcggttctg aacggccaa ccctttatg 
      gcgtctgaa gtgcttcgt ttgtttttc gaacgatcg 
      tttgtacca acttggaaa aacaaaatc gaggatttg 
      tgcctccaa gctgagtac ttaactggg agtttaagc 
      aggcctgaa aattacaaa aaagaaact tcccaagtg 
      agtcaggaa gaaattttg aaatatttc gtttggagt 
      tttatcaaa atatcagtc ttgtcgtca ccgctggaa 
      gaagatgag gaagaaaac gcaccaaaa gacatcatt 
      tacgtaact gatgaccag aaacaatta attatggat 
      gtcctttat gacctcacc atgtgtcaa tcgtttctt 
      acaaactac aaaattacg aaacattac aaaaatctg 
      tcacaatat tacgacttt atcaaggag aaaaccaca 
      caaacactt gaaaaaata caaattggt gacgaaatg 
      accgaactg ttcatctcg caactgaac tcaaattcg 
      ggcctttgg tctaaagtc atttacttg tacaaccaa 
      gaaattttc gataaaccg ccacaagaa accttcaaa 
      acataccac aaccaaacc acaagtgac ttaagccaa 
      cttcatttg gtccaactc tacggcccg cacctggat 
      ttcgaccat gtagccaca tcatcagta cttaatgac 
      gtcgtaatc ctccggagt ctcgcacat tgcgacgaa 
      atcgatgtc caaatagcg gaaattttt gaaaaaatt 
      aagaaattg cgaaatcgg gacatcccc aagtttctg 
      tttgaaaac aaagagcgg acttgcgcc gagtctgag 
      gtcgtaatc gaagtgatt cgcctattc actagactg 
      atcaaagtg aagtttgag aatttgagt cggcaacat 
      tgggacttt ggtgttatt tctctagct tcctgggct 
      tcgaattgt tgcaaattt cgcagtggt tacgacagt 
      gggaaaaac caagcgttc atggtcgca atttctgaa 
      ttttacatc gctctgaac gaaaaaatc aacgaattg 
      aagaggaat aacgtcaag agtgggttt gtggacgag 
      tgggaggaa ctcttctta cccaatgtt cacctagat 
      ttggtgcaa ttatggttg tatttagcc gaacaattt 
      gcctcaaag acgtgccta ctcgattat cacccgttc 
      ttgcaagag ttcggcaaa gttaccgat catttcgtt 
      tacaagctg attttcagt aaagaaaat acgttaccc 
      agttggtcg aaaattctc aaacgctgt gcagtgcta 
      cttacaaat tcgacgtca attttgcaa ctttgggga 
      tacaaaatg ttgctaatt ctagtacct gggcttata 
      gaaatcgat ttacagtcg gatagtagt agcacacca 
      aataccaaa gggtttacg attggacag ttcaaggaa 
      attttattg caaacgcat agtatcgta gatggcgtc 
      cttgccggt tttaagttg ggcgaggac agctgccaa 
      gttacccca tttacagat tcctacact tacgtgttt 
      gcttattta ctcctctgg ggggttttg cttagcatg 
      tgtgaaaaa tcaacaccc gagttgagg taccaatac 
      gccgattgg ttccgaaac gatgattat ttcaagtca 
      ctcttgaac aacttgttt aagttaatg ccgactgaa 
      attttgcat tacagtgag ggtaaaact aaagctttc 
      agtaagctg ttctcgcaa aatcccggt cttggtttc 
      aaagaaccc tgtactagt gaacagttg gaacacatc 
      gtctgttgg atttattcg ttctgtttg gcccaatta 
      ccggctttg gtgcgtcag tggtggtca caaactgag 
      acaaaagtg actcaaata gtcgaaaaa gtgacagcg 
      ttttacgtt tcgccgcaa ttgtgcaac caggagttg 
      ctcgatata acccaacac gaaaacaaa ttcaaaaat 
      atgacggtc aaaattgtg ccttcagtt cgtgaagtg 
      atcgcggtt tataccgtg gacgaggca cagatggag 
      ctagtggta actttaccc caaaactac cccttaggt 
      gaccctgat gtgcggtgc agcagacaa atcgggggg 
      cccttgcac aagcagtgg ctcatgcag ttgaaaaaa 
      tatctagtg caccaaaat ggccgaatt tgggatgga 
      ctatcactg tggaacagc agtcttgat aagaaattc 
      gacggtgtt gaagagtgc tacatttgt ttcgctgtt 
      ttgcatcca gggacttac cagttaccg aaactgact 
      tgtcagact tgtaaaaag aagttccat tcggcgtgt 
      ctttacaag tggttcagt acaagcaac aaaagttcg 
      tgtcctatt tgccgaaac ttgttctaa
    mRNA sequence
    • >TC011290 RA
      ttccacttt taaagtttt ataattagt gctgaagta 
      aacaatcta gtggacgct ttgggctgt tacaatggg 
      cggcaagca caagcccgc ccaaagaat caagaacaa 
      tgcgaggcc ttccagcag tgggcgcag cgccgagtt 
      gctggcgtc ctcctcaca agtgcccca attcgccgc 
      agttaagga gatccggtt tgtgccccc gaccctgtc 
      tttgctcaa aacggagga atttgactc gtccgtgtc 
      gccagcgtt tcagatcgt attcaagaa aatgaacaa 
      aaaggactg caccactaa attaaaagc cttgcagga 
      gttttgcga cctaatcgc gagttccga ggcggaggc 
      tgttaagac gatcttggc gttttgggc cacgctgta 
      tagcatttt ggccaccga cacggatca tagggtgcg 
      ggaggcggt gcacaacgc ccaccatca aatcatttt 
      gaaggataa aaaaatgat agccccctt tttgaaaca 
      gttgatagg gccctggtt cacctcaca gtatgataa 
      ttatccgcc ggcggccac ggccgcgtc taaagcttt 
      caacgccgc gtttccgca aaacaaggt ccaggattg 
      tgtggtgtt ttgccaaaa ggaaatctt gacgtacat 
      ttacgataa tttgacttc gaaaattga atctaatcc 
      taaacatgg cacaaatga ggatgctga agcgaaatt 
      tgagagaat cattatttc gagtcttca aggctactg 
      cctctattt gaatacact gactcagga acagatcga 
      gaaaagtga ggatttgaa caagcaaat tataagtaa 
      ttcaaggtt tcttaaatt ggcaacgca taaaacggc 
      acaaattcg ggccacttg gttcaaagc cgttgcgat 
      tttgtacca aaaagcccc atttttgtt agaagagga 
      acataagaa ttttgttcc ggttgtttt caataattt 
      ggatgagag tgaaccccc tgttgtggg ttctgtgtg 
      ggatgcggc tttgctcat tatggattt aggacagga 
      atggtggaa acttatcaa cattgaaaa aagttactt 
      atcaaagtt gaaaaaagt cctaaggga aggaggcca 
      aggcaacgc gaccgttat ctaccctaa tctattatc 
      acttttgca caagttgcc cccgtctgt gcgtgtgga 
      ccaatttta tgacagttt tttcgaaaa tttacgatg 
      tggattgaa gcgcaaaag tgtgataag tagtcgatc 
      cgagtcaac ggctgtggt cgcagcctt agtagaatg 
      tctacaata cgtgatatt agttaacca agaaaattg 
      cgatctgtg cgtaaaatt aataaaatc gcatttgtt 
      gcctttgat tgagtggag tttgagggc ggaacaagt 
      ctgttaccg cactgtttt ccaccaaat cgcccttct 
      agtgcagta ctggaacag aaataaaaa tctagccaa 
      ttattccga atatttggc gttgttttg gagcaacgt 
      tgtaacttt agtcgagtc agtgtcgaa gtttgagga 
      ggaaaatga ggatttggt cgatcgaca aatcgtatt 
      tttgcaaca tttaaaaac aattgccaa accaaaaga 
      acaattgaa agtgaaatt tgaagaaga agcgaggag 
      tgagtcgca cctagaggg cgccacaca aatcgatgc 
      ttcgtacaa cgaaaatct cactttgct agtctacac 
      tttgctcaa aacgtacgt tgtccaaat tgaagaaaa 
      caaatcgaa agttttaat cccgaatgt ttacgctgt 
      ggtgcgttt gttcgaaag tcgggaatt tctcgtcgg 
      tttgagcag aagtctgta ccagtcgga taatttggt 
      tactgttta tgataaatt gtttgagtg gttaaagtc 
      ggatgaatt gtcctgcga gactttggt cgatttggc 
      gtttattct tttgaatta tgttaacga tgatgagaa 
      aaatgtgat tttggagtc gtttttgca actgtccga 
      tcgtcaatt gtttggctg gtgtgtcaa aaatgcgtt 
      ctcacaccc ctacaacca ggactccag tgtccaaag 
      ttggatcaa gcggtcgga agttggacg ttttttggt 
      gtctgttgc tgattgtaa tttaaaaat tgattgccc 
      gcccgattt gaaggtttt attcaagtt ggcgttcgc 
      cgaaaatga ggatggaga attattaat tgacaaaaa 
      cactttgaa ccaaataat tgagaaagt gcgagctcc 
      gcttcttga gccccgcag ttttgaggt cgcgattga 
      ttcctgcgt ccatctagc gtcctacat taccggcac 
      catctacac cgaaaacac cctgttaaa gttcggaga 
      agacttact attggccct tttccaatt ctcctgcaa 
      cgaccacac agatttgga gtttttgac tgacgatac 
      ggaatggga ggtgaatac ggcgtggca agacgttgt 
      aatgatttt tgcgaagaa tttgcccaa aaacgaatt 
      gcaaaattt gaacggaaa attcgccga tttggttga 
      ggaaaattt cttgggaaa attcccaac agacgtaaa 
      tacgaattg tttgatcca aactatagt tagtttcat 
      caaggcggt tctgaacgg ccaaccctt tatggcgtc 
      tgaagtgct tcgtttgtt tttcgaacg atcgtttgt 
      accaacttg gaaaaacaa aatcgagga tttgtgcct 
      ccaagctga gtacttaac tgggagttt aagcaggcc 
      tgaaaatta caaaaaaga aacttccca agtgagtca 
      ggaagaaat tttgaaata tttcgtttg gagttttat 
      caaaatatc agtcttgtc gtcaccgct ggaagaaga 
      tgaggaaga aaacgcacc aaaagacat catttacgt 
      aactgatga ccagaaaca attaattat ggatgtcct 
      ttatgacct caccatgtg tcaatcgtt tcttacaaa 
      ctacaaaat tacgaaaca ttacaaaaa tctgtcaca 
      atattacga ctttatcaa ggagaaaac cacacaaac 
      acttgaaaa aatacaaat tggtgacga aatgaccga 
      actgttcat ctcgcaact gaactcaaa ttcgggcct 
      ttggtctaa agtcattta cttgtacaa ccaagaaat 
      tttcgataa accgccaca agaaacctt caaaacata 
      ccacaacca aaccacaag tgacttaag ccaacttca 
      tttggtcca actctacgg cccgcacct ggatttcga 
      ccatgtagc cacatcatc agtacttaa tgacgtcgt 
      aatcctccg gagtctcgc acattgcga cgaaatcga 
      tgtccaaat agcggaaat ttttgaaaa aattaagaa 
      attgcgaaa tcgggacat ccccaagtt tctgtttga 
      aaacaaaga gcggacttg cgccgagtc tgaggtcgt 
      aatcgaagt gattcgcct attcactag actgatcaa 
      agtgaagtt tgagaattt gagtcggca acattggga 
      ctttggtgt tatttctct agcttcctg ggcttcgaa 
      ttgttgcaa atttcgcag tggttacga cagtgggaa 
      aaaccaagc gttcatggt cgcaatttc tgaatttta 
      catcgctct gaacgaaaa aatcaacga attgaagag 
      gaataacgt caagagtgg gtttgtgga cgagtggga 
      ggaactctt cttacccaa tgttcacct agatttggt 
      gcaattatg gttgtattt agccgaaca atttgcctc 
      aaagacgtg cctactcga ttatcaccc gttcttgca 
      agagttcgg caaagttac cgatcattt cgtttacaa 
      gctgatttt cagtaaaga aaatacgtt acccagttg 
      gtcgaaaat tctcaaacg ctgtgcagt gctacttac 
      aaattcgac gtcaatttt gcaactttg gggatacaa 
      aatgttgct aattctagt acctgggct tatagaaat 
      cgatttaca gtcggatag tagtagcac accaaatac 
      caaagggtt tacgattgg acagttcaa ggaaatttt 
      attgcaaac gcatagtat cgtagatgg cgtccttgc 
      cggttttaa gttgggcga ggacagctg ccaagttac 
      cccatttac agattccta cacttacgt gtttgctta 
      tttactcct ctggggggt tttgcttag catgtgtga 
      aaaatcaac acccgagtt gaggtacca atacgccga 
      ttggttccg aaacgatga ttatttcaa gtcactctt 
      gaacaactt gtttaagtt aatgccgac tgaaatttt 
      gcattacag tgagggtaa aactaaagc tttcagtaa 
      gctgttctc gcaaaatcc cggtcttgg tttcaaaga 
      accctgtac tagtgaaca gttggaaca catcgtctg 
      ttggattta ttcgttctg tttggccca attaccggc 
      tttggtgcg tcagtggtg gtcacaaac tgagacaaa 
      agtgactca aatagtcga aaaagtgac agcgtttta 
      cgtttcgcc gcaattgtg caaccagga gttgctcga 
      tataaccca acacgaaaa caaattcaa aaatatgac 
      ggtcaaaat tgtgccttc agttcgtga agtgatcgc 
      ggtttatac cgtggacga ggcacagat ggagctagt 
      ggtaacttt accccaaaa ctacccctt aggtgaccc 
      tgatgtgcg gtgcagcag acaaatcgg ggggccctt 
      gcacaagca gtggctcat gcagttgaa aaaatatct 
      agtgcacca aaatggccg aatttggga tggactatc 
      actgtggaa cagcagtct tgataagaa attcgacgg 
      tgttgaaga gtgctacat ttgtttcgc tgttttgca 
      tccagggac ttaccagtt accgaaact gacttgtca 
      gacttgtaa aaagaagtt ccattcggc gtgtcttta 
      caagtggtt cagtacaag caacaaaag ttcgtgtcc 
      tatttgccg aaacttgtt ctaatttaa atgtaatac 
      attaattac gttgtaaaa agatttaaa taaaagttt 
      tgttaatta
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