Status
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Gene information
General information
TC015381 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 111636..114446
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015381
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09139

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015381 RA
      MSNKSAVAE FYKNRHILI TGATGFMGK VLIEKLLRS 
      CPQLSTIYL LVRPKKGKK PNERLEDII NCPVFDKLR 
      DQPDGEKLL NKIYCISGD ITQTKLNLS NDDEKTLTD 
      NVELVFHMA ANVRFDQPL KNAVLLNTG GTKNLLDLA 
      CCFKQLKIF IHVSTSYCH CNEVKLEEK LYNAPHDPR 
      KILDLVTWM DDETLKTLT PKLLKKSPN TYAYTKCLT 
      EQLVSEYKS KLPLVITRP SIVTAAWRE PIPGWVDNL 
      NGPTGLLVG AGKGVIRSM HCDPYLEAN IVPVDSAIN 
      SLLLIGWKE GAGPSRDVQ VYHVTSNND NVISWGEAL 
      EVGRKHFYD NPFSVCLWY PGGSIKSNY WYHAIAVFF 
      LHIIPAYLV DFLMVLTGN KTFLVRTQK RIQNGLEVL 
      QYYTTRPWY FYNDNLDKI WSELSDLDK EIFYTDRLK 
      IDYNQYILN YVLGARKYC VHEEPETLP YARKVLKRL 
      FYLDMTKNI VLGIFFLWI FSYYASGFI TKLDNRSIF 
      NNTTIIG
    CDS sequence
    • >TC015381 RA
      atgagcaat aaatcagcg gttgcggaa ttctacaaa 
      aaccgacac atcctcatc acgggcgcc actggtttt 
      atggggaaa gtactaatc gagaaatta ctacgctca 
      tgtcctcaa ttatcaaca atttatttg ttagttcgt 
      ccaaaaaaa gggaaaaaa cctaacgaa agacttgaa 
      gatatcatc aattgtcct gtcttcgat aagttgcgt 
      gaccaaccc gacggcgaa aaattacta aataagatt 
      tactgcatt tctggggac attacccaa actaaatta 
      aatctatca aacgatgat gagaaaact ttaacggat 
      aacgtcgaa ttggtgttt cacatggct gccaacgtg 
      cgatttgac caaccgttg aaaaacgcg gttctattg 
      aatacagga gggacaaaa aatttgcta gatctggcg 
      tgctgtttc aaacaattg aaaattttc attcatgtg 
      tcaacatca tactgtcat tgcaatgaa gtcaaactt 
      gaggaaaaa ctatacaat gcgccacat gacccgcga 
      aaaatccta gatctagtg acctggatg gacgacgaa 
      acgctaaaa actttaact ccaaaacta ctaaaaaaa 
      tcaccaaac acgtacgct tatactaaa tgtttaacc 
      gaacaattg gtgagtgaa tacaaatca aagttacct 
      ttggttatc actcgaccg tcgattgtt acggccgct 
      tggcgcgaa ccaattcct ggttgggtt gataattta 
      aatggacca acaggatta ttggtaggg gcgggtaaa 
      ggagtgatt cgatcaatg cattgtgac ccttatttg 
      gaggcaaat atcgtacca gttgacagt gcgattaat 
      tcacttctc ctgattggc tggaaggag ggggcggga 
      ccgtcacgt gacgtgcaa gtctaccac gtgacgtca 
      aacaacgac aatgtgatt agttggggg gaggcgctt 
      gaagtaggc cgtaaacat ttctatgac aacccgttt 
      agcgtttgt ttgtggtat ccaggtggt agtatcaag 
      tcgaattat tggtaccac gccatcgct gtttttttc 
      ttacacatt atcccagcc tacttggtc gattttctt 
      atggttttg acaggaaat aaaacattc ttggtgcgt 
      acccagaaa cgcatacaa aacggtttg gaggtgctc 
      cagtactat actacaaga ccttggtac ttttacaac 
      gataatttg gataagata tggagcgag ttgtcagat 
      cttgataaa gaaattttc tataccgac agacttaaa 
      atcgactac aaccaatat attttgaat tatgtgtta 
      ggtgcgagg aagtactgc gtacatgag gaaccggaa 
      acgctcccc tatgcccga aaagtacta aaaagattg 
      ttttatttg gatatgacc aaaaacatt gttttggga 
      attttcttt ttgtggatt ttttcctac tacgcatct 
      gggtttatc acaaagcta gacaaccgg tcgattttt 
      aataataca acaattata ggttaa
    mRNA sequence
    • >TC015381 RA
      ttgaaagta ttattcttg cgtaattca attctcaat 
      aataagaaa tcggatcga aacggtaca tgttgtaag 
      aagcgtgtg gaaaaatca atcgctggt gaaagtagc 
      ccagttatt taattgagc gggaaattg accaccaat 
      aagaaaata actgactaa cataatgat ccccaacac 
      gaggaattt tcatggaaa ttcccatgg tggtggtat 
      atggtaatg gtatttgta tgaaaacag gtggatggg 
      ttgcggctt tgtctacaa aacattgct gctttgtgt 
      gtgaacaat tttattgtt ttgttgctg cctttcagt 
      ttctcaaca atgagcaat aaatcagcg gttgcggaa 
      ttctacaaa aaccgacac atcctcatc acgggcgcc 
      actggtttt atggggaaa gtactaatc gagaaatta 
      ctacgctca tgtcctcaa ttatcaaca atttatttg 
      ttagttcgt ccaaaaaaa gggaaaaaa cctaacgaa 
      agacttgaa gatatcatc aattgtcct gtcttcgat 
      aagttgcgt gaccaaccc gacggcgaa aaattacta 
      aataagatt tactgcatt tctggggac attacccaa 
      actaaatta aatctatca aacgatgat gagaaaact 
      ttaacggat aacgtcgaa ttggtgttt cacatggct 
      gccaacgtg cgatttgac caaccgttg aaaaacgcg 
      gttctattg aatacagga gggacaaaa aatttgcta 
      gatctggcg tgctgtttc aaacaattg aaaattttc 
      attcatgtg tcaacatca tactgtcat tgcaatgaa 
      gtcaaactt gaggaaaaa ctatacaat gcgccacat 
      gacccgcga aaaatccta gatctagtg acctggatg 
      gacgacgaa acgctaaaa actttaact ccaaaacta 
      ctaaaaaaa tcaccaaac acgtacgct tatactaaa 
      tgtttaacc gaacaattg gtgagtgaa tacaaatca 
      aagttacct ttggttatc actcgaccg tcgattgtt 
      acggccgct tggcgcgaa ccaattcct ggttgggtt 
      gataattta aatggacca acaggatta ttggtaggg 
      gcgggtaaa ggagtgatt cgatcaatg cattgtgac 
      ccttatttg gaggcaaat atcgtacca gttgacagt 
      gcgattaat tcacttctc ctgattggc tggaaggag 
      ggggcggga ccgtcacgt gacgtgcaa gtctaccac 
      gtgacgtca aacaacgac aatgtgatt agttggggg 
      gaggcgctt gaagtaggc cgtaaacat ttctatgac 
      aacccgttt agcgtttgt ttgtggtat ccaggtggt 
      agtatcaag tcgaattat tggtaccac gccatcgct 
      gtttttttc ttacacatt atcccagcc tacttggtc 
      gattttctt atggttttg acaggaaat aaaacattc 
      ttggtgcgt acccagaaa cgcatacaa aacggtttg 
      gaggtgctc cagtactat actacaaga ccttggtac 
      ttttacaac gataatttg gataagata tggagcgag 
      ttgtcagat cttgataaa gaaattttc tataccgac 
      agacttaaa atcgactac aaccaatat attttgaat 
      tatgtgtta ggtgcgagg aagtactgc gtacatgag 
      gaaccggaa acgctcccc tatgcccga aaagtacta 
      aaaagattg ttttatttg gatatgacc aaaaacatt 
      gttttggga attttcttt ttgtggatt ttttcctac 
      tacgcatct gggtttatc acaaagcta gacaaccgg 
      tcgattttt aataataca acaattata ggttaattt 
      taattatat tcacaggtg cttggttat ttttatgta 
      ataaatttg caatttacg a
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