Status
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Gene information
General information
TC011207 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2256073..2257770
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011207
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09138

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Nearly all of them look like elongated germ band stage
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011207 RA
      MLQKSFILT SNLCKNLFV LSPTVLRKM SSVADDVIF 
      DNVGDKGVI TLNRPKALN ALNLSMVNK LYPTLVQWE 
      SEKSLVIIK GAGGKAFCA GGDVKSIAL AGMKGEKLG 
      HAFFKQEYK TDGLIGTYK VPYIAFIDG IVMGGGVGL 
      SVHGPYRVA TERSVFAMP ETQIGLFPD VGGSYFLPR 
      LRGKLGLFL ALTGYRLKG VDLLKAGIA THYVDSQCL 
      EALEGDLLK CGDSRSIDS TLSKYSTKD NSEFSLEPM 
      MKQINNCFS AGSVEEIIG RLEKDGSQF ALEMVKLLG 
      KMSPTSLKV TMKQLEYGS KMTLEECLQ MEYRIAVNC 
      LANKDFYEG VRALLIDKD QNPKWSPSK LAEVSSALV 
      ESHFAKLPE DQELRNKL
    CDS sequence
    • >TC011207 RA
      atgttgcag aaaagcttc atcttaacc tcaaacttg 
      tgcaagaac ttgttcgtc ttgagcccc accgtcttg 
      cgaaaaatg tcaagtgtt gccgatgac gtaatcttt 
      gataacgtg ggagataaa ggggtgata actttgaat 
      cgacccaag gcccttaat gcgttaaac ctctcaatg 
      gtaaataaa ttgtacccc actttggtg caatgggag 
      agcgagaag agccttgtc attatcaag ggggctggg 
      ggcaaggcc ttttgcgcc gggggcgac gttaaatcc 
      atagccttg gctggtatg aagggggaa aaactgggc 
      catgccttt tttaaacaa gagtacaaa actgacgga 
      cttattggt acttacaaa gtcccgtac attgcattt 
      attgacggg attgtcatg gggggcggc gtggggctc 
      tcagtccat ggcccttat cgcgtggcc accgaacgc 
      tcggtcttt gcgatgccg gagacgcaa atcgggctc 
      ttccccgac gttgggggc tcctacttc ctcccgcgg 
      ttaaggggc aaactgggg ctgtttcta gccctgacg 
      gggtaccgc ctcaaaggg gtcgacctc ctcaaagct 
      gggatagcg acccattac gtcgatagt caatgtttg 
      gaggctttg gagggcgat ttattgaaa tgtggggac 
      agtaggagc attgatagc actttgagt aaatacagc 
      actaaagac aatagcgag tttagcctt gaacccatg 
      atgaagcaa attaataat tgcttttcg gcgggttct 
      gttgaggag attatcggg aggttggag aaggacggg 
      tcgcagttt gccttggaa atggtcaaa ctcttgggg 
      aaaatgtca ccaacgagc cttaaggtc acgatgaaa 
      caactggaa tatgggagt aaaatgacc ttggaggag 
      tgtctacag atggagtac cgaatcgca gtcaattgt 
      ttggccaat aaggacttt tacgaaggt gtgagggcc 
      ctcctgatc gacaaagac cagaacccc aagtggagt 
      ccctcaaaa ctggccgaa gtcagttcg gcactagtc 
      gaaagccac tttgctaaa cttcctgag gaccaggag 
      ttgcgcaat aaactgtga
    mRNA sequence
    • >TC011207 RA
      tactacaat tgcaaaaat tacttaaat taggtgagt 
      gaatgttgg tttttatca ttgcaattg aagcgaaga 
      gctgacaac gcggaaatt gtgcttttt gtgaggtgc 
      ctaatatac atagtggcc caattacaa cttgtctca 
      aaggtagaa aataacagg ttttgtcgc aatactttt 
      gtcctcaca cctgtgatt tgttgtttc cctggcgaa 
      ttgtgtttg aaaataatt ttcaaaatt tgacaggtt 
      tttaaattt taaaattcg agccaagtc gcttcgtta 
      tcagtaatt tcgccacag tttggctta ataataaca 
      tgtgattcc ctaatcagt gtcttcttg cctataaat 
      tgcttgaaa attgtaatt ttgtgcttt gtgaacttt 
      gaccttgtc tggtgtttt tgaaaacct tcagtgtag 
      caccaatac aaaatgttg cagaaaagc ttcatctta 
      acctcaaac ttgtgcaag aacttgttc gtcttgagc 
      cccaccgtc ttgcgaaaa atgtcaagt gttgccgat 
      gacgtaatc tttgataac gtgggagat aaaggggtg 
      ataactttg aatcgaccc aaggccctt aatgcgtta 
      aacctctca atggtaaat aaattgtac cccactttg 
      gtgcaatgg gagagcgag aagagcctt gtcattatc 
      aagggggct gggggcaag gccttttgc gccgggggc 
      gacgttaaa tccatagcc ttggctggt atgaagggg 
      gaaaaactg ggccatgcc ttttttaaa caagagtac 
      aaaactgac ggacttatt ggtacttac aaagtcccg 
      tacattgca tttattgac gggattgtc atggggggc 
      ggcgtgggg ctctcagtc catggccct tatcgcgtg 
      gccaccgaa cgctcggtc tttgcgatg ccggagacg 
      caaatcggg ctcttcccc gacgttggg ggctcctac 
      ttcctcccg cggttaagg ggcaaactg gggctgttt 
      ctagccctg acggggtac cgcctcaaa ggggtcgac 
      ctcctcaaa gctgggata gcgacccat tacgtcgat 
      agtcaatgt ttggaggct ttggagggc gatttattg 
      aaatgtggg gacagtagg agcattgat agcactttg 
      agtaaatac agcactaaa gacaatagc gagtttagc 
      cttgaaccc atgatgaag caaattaat aattgcttt 
      tcggcgggt tctgttgag gagattatc gggaggttg 
      gagaaggac gggtcgcag tttgccttg gaaatggtc 
      aaactcttg gggaaaatg tcaccaacg agccttaag 
      gtcacgatg aaacaactg gaatatggg agtaaaatg 
      accttggag gagtgtcta cagatggag taccgaatc 
      gcagtcaat tgtttggcc aataaggac ttttacgaa 
      ggtgtgagg gccctcctg atcgacaaa gaccagaac 
      cccaagtgg agtccctca aaactggcc gaagtcagt 
      tcggcacta gtcgaaagc cactttgct aaacttcct 
      gaggaccag gagttgcgc aataaactg tgattttgt 
      tatttgcaa taaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY