Status
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Gene information
General information
TC014802 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15277078..15281522
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014802
    iBeetle screen iB_09136

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014802 RA
      MPSSLLFGI NSEGRVHTL STTGTMWRE IPYAGQEFK 
      KLSAVPHFL WALGGDHQI YVYVHGLDI PIRVREESY 
      ENERWLPIE GFSSRLLPT DRYHFSNGD GTVDRSIDK 
      IRLPSMAWQ WEGDWQLEL TLDGQPLDH QGWTYAVDF 
      PSQYYSKKQ WKSCVRRRL WVRSRRYSA MNSWCAIAP 
      LHKDATSEP FIDISIGGQ SVAGAEPGT MLVWAVTSH 
      NRVMFRTGV STKSPEGAR WNPIQVPLG SEVNQINVG 
      PTGLVWASL LDGRALVRT GVTRDCLTG DSWVEVRGP 
      GDNLKISQL SVGSCAVWA ITQNKQVWF RKGVKGEGA 
      GMSEELAVG CGWVEMVGR MAQISVAAN DQVFAVGAD 
      DRLVYYRSG VANSDLTGK RWKALHAPL QVSRASSNA 
      SLNRDKFHR SFNALQSRP SSMCEPSTI SEWEDHSHS 
      APTAPTSLP VGDLTQKFE TQPKNPKAW SPVHSVGSI 
      VGTEVHPEV DESVWSDGS RESCIFAED EELGWAEYE 
      APWSWVEAG ACTVDPTQL PNWFAETGG GASQTEIGE 
      PWRLKILQE LKLRLPTEK YKNWQLAVD TTSWVHSGE 
      ARVSWGSGQ FVDCVLQLE WVQTTGTLT VLNPDGAST 
      MNLFSLNDI TCVQCCSEP GSPRLALHV PRHAPSLLR 
      LQFSSDAQL EEWQTHFAT TCGKLHQAL GKPSEESVW 
      AVTGLGDVF IWDPTQLES NQLRENDYY VQKFDLSGK 
      ESPIKVALH GSFNPGTTL TLTGCIGDD ADRIGINLD 
      AHSTYKLRH KAYSEFENT CLHFNPRFT DGYIVRNSM 
      IEGKWGKEE CEGEMPLQK GHEFTLKIE TTEDAFVIY 
      IDDKLFTHY RHRLPPNSV SMLNIWGRL QPFRLVIKC 
      PDIILDPLN LYWRQVGGH LRRVESCRV GVTWGIGYD 
      HTAWVYTGG WGGGFLGPL DSHNVHPMT DSQDYRVYE 
      NQRWNPVTG YTSAGLPTD RYMWSDATG KQKRTRDQV 
      KLLSVKWQW VSDWMIDFH VPGGVDRDG WQYAVDFPS 
      TYHAHKNFT DYVRRRRWY RRCAVATTG PWQELGHTK 
      LIDVSLEPV KDDLDSVIS VWALASGGQ AMFRTGVTR 
      SNPTGTGWE HVTSDQPLA SISCGSYNL VWATGKKGC 
      AYWRAGINE NKLEGERWV CVEPPNGGQ LKQISVNCV 
      GVWGVDHLG RLHVRREVT ETFPEGTHW QTITVDPLV 
      LNSAPSTTG FKHVSVGRN EVWATTDGG AMLRRTGIC 
      KTNPGGSGW FIGIPGNWQ HVSVRAFN
    CDS sequence
    • >TC014802 RA
      atgcccagt tctctcctt tttggcata aacagcgag 
      gggcgcgtt cacaccctc tccactacg gggacaatg 
      tggcgcgaa attccttac gctggtcaa gaatttaaa 
      aaattgtcg gctgttccg cacttcctt tgggcctta 
      ggaggcgac caccaaatc tacgtgtac gtccacggt 
      ctcgacatt cccatccgg gtccgagag gagtcctac 
      gagaacgag aggtggctt ccaattgaa ggattttcc 
      tcgcgtctt ttaccaaca gatcgctat catttttcg 
      aatggtgac ggtactgtt gatcgctca attgataaa 
      atcaggttg ccttcaatg gcctggcaa tgggagggg 
      gattggcag ctggagttg actcttgat ggacagccc 
      ctagaccac caagggtgg acttatgct gttgatttc 
      ccgtcacaa tattactcc aagaagcag tggaagagc 
      tgtgtcagg cggagactg tgggtgagg tcgcgcagg 
      tacagcgcg atgaattcg tggtgcgcg atagccccc 
      ttacacaag gacgctaca agtgagcca ttcattgac 
      atatcaatc ggggggcaa tcggtggcg ggggctgag 
      cccgggact atgctagtt tgggctgta acttcacac 
      aaccgggtc atgtttcga actggcgtt agtacgaaa 
      tcccccgaa ggcgcccgc tggaacccc atccaagtg 
      cctctcggc agcgaagtc aaccaaatc aacgtgggc 
      cccacgggg ctagtttgg gcttcgctc ctcgacggg 
      cgcgccctt gtccgtacc ggagtcacg cgtgattgt 
      ctcacgggt gactcgtgg gtggaagtg cggggcccc 
      ggagataat ctcaaaata agccagctt tcggtgggc 
      agttgtgcc gtttgggca atcacccaa aacaagcaa 
      gtctggttt aggaaaggg gtcaaaggc gagggtgct 
      ggcatgagc gaggagttg gctgttggg tgtggatgg 
      gtggaaatg gtcggacgg atggcacag attagtgtt 
      gccgccaat gaccaggtc ttcgcagtg ggggctgat 
      gatcgcctc gtttattat cggagcgga gtggccaac 
      tcggatttg acggggaag aggtggaag gcactgcat 
      gcacccttg caagtcagt agggcgagc agtaatgcg 
      agtcttaat agagataag tttcatcgc agctttaac 
      gctttgcag agccgccct agcagcatg tgcgagccg 
      tctacgatt tcggaatgg gaggaccac tcccactcc 
      gcccccaca gcccccacg tccctcccc gtgggcgac 
      ctaacccaa aaatttgaa acacaacca aagaacccc 
      aaagcttgg agtcccgtc cattcggtg ggctccatt 
      gtcggtacc gaagtccac ccagaagtg gacgaaagt 
      gtctggagt gacggaagt cgcgaatcg tgtattttc 
      gccgaagac gaagaatta ggttgggcg gagtatgaa 
      gcaccctgg tcgtgggta gaggcgggc gcttgtacc 
      gtcgatccc acccaactt cctaattgg ttcgccgaa 
      accggaggc ggtgctagt caaaccgaa atcggtgaa 
      ccatggcgt ttgaaaatc ttacaagaa ttaaaactg 
      cgactaccc actgaaaag tacaagaat tggcaattg 
      gccgttgat acaacatcg tgggtgcat tcaggggag 
      gcgcgtgtg agttggggg agtggccag tttgttgat 
      tgtgtgcta cagttggag tgggtgcag accactggg 
      actttaact gtgttaaat ccggatggg gcgagtact 
      atgaatctt ttcagtttg aatgacatc acgtgtgtc 
      caatgttgt agcgagccg gggagtcct cggctcgcg 
      cttcatgtg ccacgccac gccccttca ttactgagg 
      ttacagttt agtagtgac gcccagttg gaggagtgg 
      caaacgcat tttgcgacc acttgtggc aaattgcat 
      caagcgtta ggaaaaccg agtgaggag agtgtgtgg 
      gcggttacg ggtttaggg gatgtgttt atttgggac 
      cccacccaa ctcgaatcg aaccaattg cgggagaat 
      gattattat gtacaaaag tttgacttg agtgggaaa 
      gaatcgccg attaaagtt gcgcttcat gggagtttt 
      aatcccggg acaacgcta actttaact gggtgtata 
      ggggatgat gcggaccga attggaatt aatttggac 
      gcccactcg acttataaa ttgcggcat aaagcctac 
      agcgagttt gaaaatact tgtcttcat ttcaacccg 
      aggtttact gatggttac attgtgcgt aattctatg 
      attgaaggc aagtggggg aaggaggag tgtgagggg 
      gaaatgccg ttgcaaaaa gggcacgaa ttcaccctc 
      aaaatcgag acaacagaa gacgccttt gttatttac 
      atcgatgat aagctcttt acgcattat aggcaccgc 
      ctaccgcca aattcagtc tcgatgttg aacatttgg 
      gggcgttta caaccattc cgattggtt atcaagtgt 
      cctgatatt attctcgac ccgttaaat ttgtattgg 
      aggcaagtg ggtgggcac ttgaggcgt gtcgaatca 
      tgtcgagtg ggcgtaact tggggcata gggtacgac 
      cacacggcg tgggtgtac accggtggg tggggcggt 
      ggttttttg ggacctttg gacagtcat aatgttcat 
      cctatgact gattcgcaa gattatcgc gtttacgaa 
      aaccaaaga tggaatcct gtcactgga tacacttca 
      gcagggtta ccaactgat cgttacatg tggagcgat 
      gccactggc aaacaaaaa agaaccaga gatcaagtg 
      aaactcctg tcggtgaaa tggcagtgg gtttccgat 
      tggatgatt gattttcac gtgccaggg ggcgtggac 
      agggacggc tggcagtat gcggttgat tttccgagt 
      acttaccat gcccataaa aatttcacc gattatgtg 
      aggcggagg cggtggtat aggcggtgt gctgttgcc 
      acaaccggg ccctggcaa gaattaggc cacaccaaa 
      ttgattgat gtgtcattg gagccagtc aaagatgat 
      ttggatagt gtcatatcg gtgtgggcg ttggcttca 
      ggaggccaa gccatgttc cggactgga gtcacgaga 
      tcaaatcct acaggaaca ggatgggaa catgtcacc 
      tccgaccaa ccacttgcc agtatcagt tgtgggtcc 
      tacaacctg gtttgggcc actggcaaa aaagggtgt 
      gcctattgg agggcggga attaacgaa aataagctc 
      gaaggggag aggtgggtg tgtgtggaa cctcccaac 
      ggtgggcaa ctgaagcaa atttcggtg aactgtgtt 
      ggggtttgg ggggttgat catttaggg aggttgcat 
      gtgaggagg gaagtgacg gagacgttt cccgaagga 
      acgcattgg caaacgata acagtcgat ccgcttgta 
      cttaattca gctccgagt acaaccgga tttaaacat 
      gtaagtgtt gggagaaat gaagtatgg gctacgacc 
      gatggtggc gccatgcta cgacgtacg ggcatttgt 
      aaaactaac ccgggagga tcgggatgg tttatcgga 
      ataccgggg aactggcaa catgtcagc gtccgggca 
      tttaactaa
    mRNA sequence
    • >TC014802 RA
      ttggttatg tgtgtcggt gtgggtgcg atgaaattg 
      taaacattc caaaatgcc cagttctct cctttttgg 
      cataaacag cgaggggcg cgttcacac cctctccac 
      tacggggac aatgtggcg cgaaattcc ttacgctgg 
      tcaagaatt taaaaaatt gtcggctgt tccgcactt 
      cctttgggc cttaggagg cgaccacca aatctacgt 
      gtacgtcca cggtctcga cattcccat ccgggtccg 
      agaggagtc ctacgagaa cgagaggtg gcttccaat 
      tgaaggatt ttcctcgcg tcttttacc aacagatcg 
      ctatcattt ttcgaatgg tgacggtac tgttgatcg 
      ctcaattga taaaatcag gttgccttc aatggcctg 
      gcaatggga gggggattg gcagctgga gttgactct 
      tgatggaca gcccctaga ccaccaagg gtggactta 
      tgctgttga tttcccgtc acaatatta ctccaagaa 
      gcagtggaa gagctgtgt caggcggag actgtgggt 
      gaggtcgcg caggtacag cgcgatgaa ttcgtggtg 
      cgcgatagc ccccttaca caaggacgc tacaagtga 
      gccattcat tgacatatc aatcggggg gcaatcggt 
      ggcgggggc tgagcccgg gactatgct agtttgggc 
      tgtaacttc acacaaccg ggtcatgtt tcgaactgg 
      cgttagtac gaaatcccc cgaaggcgc ccgctggaa 
      ccccatcca agtgcctct cggcagcga agtcaacca 
      aatcaacgt gggccccac ggggctagt ttgggcttc 
      gctcctcga cgggcgcgc ccttgtccg taccggagt 
      cacgcgtga ttgtctcac gggtgactc gtgggtgga 
      agtgcgggg ccccggaga taatctcaa aataagcca 
      gctttcggt gggcagttg tgccgtttg ggcaatcac 
      ccaaaacaa gcaagtctg gtttaggaa aggggtcaa 
      aggcgaggg tgctggcat gagcgagga gttggctgt 
      tgggtgtgg atgggtgga aatggtcgg acggatggc 
      acagattag tgttgccgc caatgacca ggtcttcgc 
      agtgggggc tgatgatcg cctcgttta ttatcggag 
      cggagtggc caactcgga tttgacggg gaagaggtg 
      gaaggcact gcatgcacc cttgcaagt cagtagggc 
      gagcagtaa tgcgagtct taatagaga taagtttca 
      tcgcagctt taacgcttt gcagagccg ccctagcag 
      catgtgcga gccgtctac gatttcgga atgggagga 
      ccactccca ctccgcccc cacagcccc cacgtccct 
      ccccgtggg cgacctaac ccaaaaatt tgaaacaca 
      accaaagaa ccccaaagc ttggagtcc cgtccattc 
      ggtgggctc cattgtcgg taccgaagt ccacccaga 
      agtggacga aagtgtctg gagtgacgg aagtcgcga 
      atcgtgtat tttcgccga agacgaaga attaggttg 
      ggcggagta tgaagcacc ctggtcgtg ggtagaggc 
      gggcgcttg taccgtcga tcccaccca acttcctaa 
      ttggttcgc cgaaaccgg aggcggtgc tagtcaaac 
      cgaaatcgg tgaaccatg gcgtttgaa aatcttaca 
      agaattaaa actgcgact acccactga aaagtacaa 
      gaattggca attggccgt tgatacaac atcgtgggt 
      gcattcagg ggaggcgcg tgtgagttg ggggagtgg 
      ccagtttgt tgattgtgt gctacagtt ggagtgggt 
      gcagaccac tgggacttt aactgtgtt aaatccgga 
      tggggcgag tactatgaa tcttttcag tttgaatga 
      catcacgtg tgtccaatg ttgtagcga gccggggag 
      tcctcggct cgcgcttca tgtgccacg ccacgcccc 
      ttcattact gaggttaca gtttagtag tgacgccca 
      gttggagga gtggcaaac gcattttgc gaccacttg 
      tggcaaatt gcatcaagc gttaggaaa accgagtga 
      ggagagtgt gtgggcggt tacgggttt aggggatgt 
      gtttatttg ggaccccac ccaactcga atcgaacca 
      attgcggga gaatgatta ttatgtaca aaagtttga 
      cttgagtgg gaaagaatc gccgattaa agttgcgct 
      tcatgggag ttttaatcc cgggacaac gctaacttt 
      aactgggtg tatagggga tgatgcgga ccgaattgg 
      aattaattt ggacgccca ctcgactta taaattgcg 
      gcataaagc ctacagcga gtttgaaaa tacttgtct 
      tcatttcaa cccgaggtt tactgatgg ttacattgt 
      gcgtaattc tatgattga aggcaagtg ggggaagga 
      ggagtgtga gggggaaat gccgttgca aaaagggca 
      cgaattcac cctcaaaat cgagacaac agaagacgc 
      ctttgttat ttacatcga tgataagct ctttacgca 
      ttataggca ccgcctacc gccaaattc agtctcgat 
      gttgaacat ttgggggcg tttacaacc attccgatt 
      ggttatcaa gtgtcctga tattattct cgacccgtt 
      aaatttgta ttggaggca agtgggtgg gcacttgag 
      gcgtgtcga atcatgtcg agtgggcgt aacttgggg 
      catagggta cgaccacac ggcgtgggt gtacaccgg 
      tgggtgggg cggtggttt tttgggacc tttggacag 
      tcataatgt tcatcctat gactgattc gcaagatta 
      tcgcgttta cgaaaacca aagatggaa tcctgtcac 
      tggatacac ttcagcagg gttaccaac tgatcgtta 
      catgtggag cgatgccac tggcaaaca aaaaagaac 
      cagagatca agtgaaact cctgtcggt gaaatggca 
      gtgggtttc cgattggat gattgattt tcacgtgcc 
      agggggcgt ggacaggga cggctggca gtatgcggt 
      tgattttcc gagtactta ccatgccca taaaaattt 
      caccgatta tgtgaggcg gaggcggtg gtataggcg 
      gtgtgctgt tgccacaac cgggccctg gcaagaatt 
      aggccacac caaattgat tgatgtgtc attggagcc 
      agtcaaaga tgatttgga tagtgtcat atcggtgtg 
      ggcgttggc ttcaggagg ccaagccat gttccggac 
      tggagtcac gagatcaaa tcctacagg aacaggatg 
      ggaacatgt cacctccga ccaaccact tgccagtat 
      cagttgtgg gtcctacaa cctggtttg ggccactgg 
      caaaaaagg gtgtgccta ttggagggc gggaattaa 
      cgaaaataa gctcgaagg ggagaggtg ggtgtgtgt 
      ggaacctcc caacggtgg gcaactgaa gcaaatttc 
      ggtgaactg tgttggggt ttggggggt tgatcattt 
      agggaggtt gcatgtgag gagggaagt gacggagac 
      gtttcccga aggaacgca ttggcaaac gataacagt 
      cgatccgct tgtacttaa ttcagctcc gagtacaac 
      cggatttaa acatgtaag tgttgggag aaatgaagt 
      atgggctac gaccgatgg tggcgccat gctacgacg 
      tacgggcat ttgtaaaac taacccggg aggatcggg 
      atggtttat cggaatacc ggggaactg gcaacatgt 
      cagcgtccg ggcatttaa ctaagtgct aaaatgtga 
      ttttatttt ttttaacca gctttattt ctgtgatag 
      ttttatttg aaagaaaag gcaagctta actcataaa 
      ttattttat attattttt tgttgtaat tataaacat 
      tccatataa acccaacac atataaaca ataaactgt 
      tacaaaaaa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY