Status
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Gene information
General information
TC013011 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15273501..15276736
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013011
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09135

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013011 RA
      MAEEDLEPS ELDKLLEKT DLEYMLEFE TQIFLDILH 
      KDGLVVAAK GLNLDLVLL NLFKVYSDP GNLVMVLNA 
      SEAEEKFFI NKIKDGNVH STTFNVSIT DREDAYLSG 
      GIHFITTRI LVVDLLKNR VPIDKITGF VILRAHKVL 
      ESCQEAFAL RLYRQSNKT GFIKAFSNS VQSFTMGFG 
      HVERIMRTI FVKELYIWP RFHSMVIQS LKQYEPQVI 
      ELHIPISEN MSKMQTYIL ELMNITVKE LKRINKTVE 
      LQEITVENC LTKKFQKNL QMQLDGIWN QLSEKSKQL 
      VADLKTLRH LIITMHYAD PVTFYSTLS SYRTMEYAQ 
      TATWVLTEP AELLFSQAS SLIFTGDKE LNPEFCPKW 
      KSLLELLKV EIPHEIEKM KTRENKILI LCSDQKTCY 
      QLSQLLTHG PHKYLFFNA LDKKVTFKK INDSFQQCG 
      KIEEGPEAA KKPKLDESN VNESEEIKS SYVLTMSQT 
      PGDDSQYIF EQCEEWENM NITQICQSL TAPTVLIQT 
      FKGTPNLQR TLEEINPSF IIMYHSSIT AIREIEMFE 
      AHRKRETSL KTYFLIHGE TVEEQSYLT SLRREKEAF 
      EYLIETKSK MVVPEDQDG KSDLCLTLQ RDLKSPSKS 
      TRQGGRPEE APRKYVIVD MREFRSDLP ALIHKRGLD 
      IEPVTITVG DYILTPEIC VERKSLSDL VGSLNSGRL 
      YQQCTQMFR YYAKPMLLI EFDQNKPFS WQNQYMVSS 
      DTNSFDIQK KLLLLTLHF PKLKIIWSP SPYATAQLF 
      EELKEGKEE PNVEYAAAI GGNQDLDLV ETKYNSNIY 
      DFVQKLPGI NSKNIDTFL RRVKSMDNA VKMSENELR 
      EVLGNPNDA RALYGALHN DQNPKELET KTKGQWARK 
      RFAKKK
    CDS sequence
    • >TC013011 RA
      atggctgaa gaggattta gagccctcg gagttggat 
      aaacttctc gaaaagaca gatctcgag tacatgctc 
      gaatttgaa acacaaata ttccttgat attcttcac 
      aaagatggt ttagtcgta gcagccaaa ggtctcaac 
      ctcgatttg gtcctttta aaccttttc aaagtttac 
      agcgatcca ggtaaccta gttatggtc ttgaacgca 
      agcgaagcg gaagaaaag ttcttcata aataaaatc 
      aaagacggt aatgttcat agcacaacg tttaacgtc 
      agcatcact gatagagag gatgcttat ttatcagga 
      ggtatccat tttatcaca accagaatt ttggttgtt 
      gatttgctc aaaaatcgt gtccctatt gataaaatc 
      acaggtttt gttattttg cgcgctcat aaggtccta 
      gagagttgc caagaggcg tttgcgttg cgtttgtac 
      cgccaaagc aataaaacg ggtttcatt aaggcattt 
      tcaaacagt gtccaaagt tttacaatg ggatttggt 
      cacgtcgaa cgcataatg cggacgata tttgtcaaa 
      gaattgtac atctggcct cgatttcat tctatggtc 
      attcaaagt ttgaagcaa tatgaacca caagtgatt 
      gagctacat atccccatt tcggaaaat atgagcaaa 
      atgcaaact tacattttg gagttgatg aacattacc 
      gttaaagaa ttgaaaaga attaacaaa actgtggaa 
      ctgcaagaa attaccgtt gaaaattgt ttgactaaa 
      aaattccaa aaaaactta caaatgcaa ctggacggt 
      atttggaac cagttgagc gaaaaaagt aaacaacta 
      gtggcggat ttgaaaacg ttacgtcat ttgataatt 
      acaatgcat tatgccgat cctgttaca ttttactca 
      actttatca agctacagg acaatggaa tatgcccaa 
      acggccact tgggtcctt actgaacct gcagaatta 
      cttttttct caagcaagt tcgttgatt tttacagga 
      gacaaagaa cttaatccc gaattttgt cccaaatgg 
      aagtctctg ttggagctt cttaaagtc gaaatacct 
      cacgaaatc gaaaaaatg aaaacacgc gaaaataaa 
      attttaata ctgtgttca gaccaaaaa acatgttat 
      cagttaagt cagttatta acgcacggg ccgcacaaa 
      tatttattc tttaatgcc cttgataag aaagtcact 
      tttaaaaaa atcaacgat tcattccaa caatgtggt 
      aaaatagag gagggtcca gaagcagcc aaaaaaccc 
      aaattagac gaaagtaac gtcaacgaa tcggaagaa 
      ataaaaagt agttatgtc ctaaccatg tcacaaaca 
      cccggtgac gattctcaa tacattttc gaacaatgc 
      gaagagtgg gaaaacatg aacataacc caaatttgt 
      caatcccta acagccccc acagtcctc atccaaacc 
      ttcaaaggc actcccaac ctccaacgc acccttgaa 
      gagatcaac ccttctttc atcatcatg taccatagc 
      agtatcaca gcaatccgc gaaattgaa atgtttgaa 
      gcgcaccgg aaacgcgaa acgtccctc aaaacctac 
      ttcctgatc catggcgaa acagttgaa gaacaaagc 
      tatctaact tcactacgg cgtgaaaaa gaagccttc 
      gagtatttg attgaaacc aaatcgaaa atggtggtg 
      cctgaagac caagacggt aaatccgac ctctgtctc 
      accctccaa cgcgatctc aaatccccc agtaaaagt 
      acgagacaa ggggggcgc cctgaggag gccccccgg 
      aagtatgtt atagtcgac atgagggag tttcggagc 
      gatttgccc gctcttatc cacaaaagg gggctagat 
      atagaaccg gttacgatc acagtgggg gactatatt 
      ctcacaccg gagatttgt gttgaacgg aagagtctt 
      tcggacctg gtggggtcc cttaacagt ggtagattg 
      taccaacag tgcacccaa atgtttagg tattatgcc 
      aaacccatg cttttgatt gagtttgac caaaataag 
      ccgttctct tggcagaac caatatatg gtctcaagt 
      gatacgaat agtttcgat attcagaaa aaactgctt 
      ttgctgacg ttacatttt ccgaaattg aaaatcatt 
      tggagtcct agtccctat gcgacggct cagttgttt 
      gaagaattg aaggaagga aaggaggaa ccaaatgtg 
      gaatacgcc gccgcgatc ggaggcaac caagacctg 
      gacctagtc gagaccaaa tacaacagt aacatttac 
      gattttgtc caaaaactc cctggaatt aactcaaaa 
      aacatcgac acgtttcta cgccgggtg aaaagtatg 
      gacaacgcg gttaaaatg agcgaaaac gaactgaga 
      gaagtattg ggtaatcca aacgacgct cgtgcttta 
      tacggggcc ctccacaac gaccaaaac cccaaagaa 
      ctcgaaact aagactaaa ggtcaatgg gcgcgaaag 
      cgcttcgcc aagaagaaa taa
    mRNA sequence
    • >TC013011 RA
      aaccaaaaa aagtgtcaa caatttttg aatttggaa 
      agtcgcttt tcgctaaaa actatggct gaagaggat 
      ttagagccc tcggagttg gataaactt ctcgaaaag 
      acagatctc gagtacatg ctcgaattt gaaacacaa 
      atattcctt gatattctt cacaaagat ggtttagtc 
      gtagcagcc aaaggtctc aacctcgat ttggtcctt 
      ttaaacctt ttcaaagtt tacagcgat ccaggtaac 
      ctagttatg gtcttgaac gcaagcgaa gcggaagaa 
      aagttcttc ataaataaa atcaaagac ggtaatgtt 
      catagcaca acgtttaac gtcagcatc actgataga 
      gaggatgct tatttatca ggaggtatc cattttatc 
      acaaccaga attttggtt gttgatttg ctcaaaaat 
      cgtgtccct attgataaa atcacaggt tttgttatt 
      ttgcgcgct cataaggtc ctagagagt tgccaagag 
      gcgtttgcg ttgcgtttg taccgccaa agcaataaa 
      acgggtttc attaaggca ttttcaaac agtgtccaa 
      agttttaca atgggattt ggtcacgtc gaacgcata 
      atgcggacg atatttgtc aaagaattg tacatctgg 
      cctcgattt cattctatg gtcattcaa agtttgaag 
      caatatgaa ccacaagtg attgagcta catatcccc 
      atttcggaa aatatgagc aaaatgcaa acttacatt 
      ttggagttg atgaacatt accgttaaa gaattgaaa 
      agaattaac aaaactgtg gaactgcaa gaaattacc 
      gttgaaaat tgtttgact aaaaaattc caaaaaaac 
      ttacaaatg caactggac ggtatttgg aaccagttg 
      agcgaaaaa agtaaacaa ctagtggcg gatttgaaa 
      acgttacgt catttgata attacaatg cattatgcc 
      gatcctgtt acattttac tcaacttta tcaagctac 
      aggacaatg gaatatgcc caaacggcc acttgggtc 
      cttactgaa cctgcagaa ttacttttt tctcaagca 
      agttcgttg atttttaca ggagacaaa gaacttaat 
      cccgaattt tgtcccaaa tggaagtct ctgttggag 
      cttcttaaa gtcgaaata cctcacgaa atcgaaaaa 
      atgaaaaca cgcgaaaat aaaatttta atactgtgt 
      tcagaccaa aaaacatgt tatcagtta agtcagtta 
      ttaacgcac gggccgcac aaatattta ttctttaat 
      gcccttgat aagaaagtc acttttaaa aaaatcaac 
      gattcattc caacaatgt ggtaaaata gaggagggt 
      ccagaagca gccaaaaaa cccaaatta gacgaaagt 
      aacgtcaac gaatcggaa gaaataaaa agtagttat 
      gtcctaacc atgtcacaa acacccggt gacgattct 
      caatacatt ttcgaacaa tgcgaagag tgggaaaac 
      atgaacata acccaaatt tgtcaatcc ctaacagcc 
      cccacagtc ctcatccaa accttcaaa ggcactccc 
      aacctccaa cgcaccctt gaagagatc aacccttct 
      ttcatcatc atgtaccat agcagtatc acagcaatc 
      cgcgaaatt gaaatgttt gaagcgcac cggaaacgc 
      gaaacgtcc ctcaaaacc tacttcctg atccatggc 
      gaaacagtt gaagaacaa agctatcta acttcacta 
      cggcgtgaa aaagaagcc ttcgagtat ttgattgaa 
      accaaatcg aaaatggtg gtgcctgaa gaccaagac 
      ggtaaatcc gacctctgt ctcaccctc caacgcgat 
      ctcaaatcc cccagtaaa agtacgaga caagggggg 
      cgccctgag gaggccccc cggaagtat gttatagtc 
      gacatgagg gagtttcgg agcgatttg cccgctctt 
      atccacaaa agggggcta gatatagaa ccggttacg 
      atcacagtg ggggactat attctcaca ccggagatt 
      tgtgttgaa cggaagagt ctttcggac ctggtgggg 
      tcccttaac agtggtaga ttgtaccaa cagtgcacc 
      caaatgttt aggtattat gccaaaccc atgcttttg 
      attgagttt gaccaaaat aagccgttc tcttggcag 
      aaccaatat atggtctca agtgatacg aatagtttc 
      gatattcag aaaaaactg cttttgctg acgttacat 
      tttccgaaa ttgaaaatc atttggagt cctagtccc 
      tatgcgacg gctcagttg tttgaagaa ttgaaggaa 
      ggaaaggag gaaccaaat gtggaatac gccgccgcg 
      atcggaggc aaccaagac ctggaccta gtcgagacc 
      aaatacaac agtaacatt tacgatttt gtccaaaaa 
      ctccctgga attaactca aaaaacatc gacacgttt 
      ctacgccgg gtgaaaagt atggacaac gcggttaaa 
      atgagcgaa aacgaactg agagaagta ttgggtaat 
      ccaaacgac gctcgtgct ttatacggg gccctccac 
      aacgaccaa aaccccaaa gaactcgaa actaagact 
      aaaggtcaa tgggcgcga aagcgcttc gccaagaag 
      aaataatac ggtcattcg tgtacatat tattttttt 
      aaataaata aataagcaa gctataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY