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Gene information
General information
TC014772 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14857007..14863787
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014772
    iBeetle screen iB_09131

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014772 RA
      MMMTQRGPG PPYNVIFSL VILVLACTT FAVGEKSKY 
      YSMQSLCKN HFLQQLYRK IDGAVLWSK NERNLDCVI 
      TFQTHSILQ RFMLRFDSL QLDCNDHLY IYDGAHAVG 
      TYKFDLSCR NTKQTLGAM FTRTNYVTL KYVTDAWGT 
      NDNGFTLVI TAVKDPKHA CTEFRCNLR EFCIATDLV 
      CDSINHCAD GSDESSNTL CQNNETGTI LGLEMTWFV 
      VTVISTLLV LLVCIVTVS ICLCQRGWS ATTRSGNGN 
      VAQPNAPYP LQQMYGSNG AMKQGASMT TLTSAGAAT 
      ATAVQGNWR HPAGPLGFR LSTPARARL YCQANSRSG 
      RVVACRVSR VQNPALGQQ HQSTVSHVT SPHCVFDYP 
      KDCIYV
    CDS sequence
    • >TC014772 RA
      atgatgatg acacagaga ggacctgga cctccgtat 
      aatgtgatc ttttctctc gttattttg gtcctagca 
      tgcaccacg ttcgcagtc ggcgaaaaa agcaaatat 
      tattccatg cagtcgttg tgcaagaac catttcctg 
      cagcaactc taccggaaa atcgacggt gccgttctg 
      tggtcaaaa aacgagcga aatttggat tgcgttatc 
      acgtttcag acacactcg attttgcag cggtttatg 
      ctccggttc gactccttg caacttgac tgcaacgac 
      catctgtac atctatgat ggggcgcat gccgtcggc 
      acgtacaag tttgacctc tcctgcaga aatacaaag 
      caaacactg ggggccatg ttcactcgc accaattac 
      gttactctc aaatacgta acggacgct tggggcacc 
      aacgacaac ggctttacg ctcgtcatc acggcagtg 
      aaggacccg aagcatgcc tgcactgag tttcgctgc 
      aacttgcgt gagttttgt atagccacg gacttggtc 
      tgcgacagc atcaatcac tgtgcggac ggctctgat 
      gaatcttca aacacttta tgtcagaat aacgaaacg 
      ggaacaatc ctcggcttg gaaatgacg tggtttgtt 
      gtaacagta attagcact ttgttagtt ttgttagtt 
      tgcattgtt accgtttcc atatgttta tgtcagcgc 
      ggctggagc gccacgacc agatccgga aacggaaac 
      gtggcccaa ccgaacgcc ccataccca cttcagcag 
      atgtacggt tcgaatggt gcgatgaaa cagggggct 
      agcatgacg actttgaca agtgctggc gccgccact 
      gcaaccgcc gttcaggga aactggcgc caccctgcg 
      ggcccacta gggttccgc ctcagcacg ccggcccgg 
      gcccgcctc tactgccag gcaaacagt agaagcggc 
      cgagtcgta gcctgcagg gtgagtcga gtgcagaac 
      ccagcgctc ggccaacag caccaatcg actgtcagc 
      cacgtgacc tctcctcac tgtgtgttt gactatcca 
      aaggactgt atttacgtt tag
    mRNA sequence
    • >TC014772 RA
      cttttaaaa tcacaatct aaaacagat tcgtatcgt 
      tacaaccga catacatat cgcgtccgt accatatca 
      gtgaatttt tgaaaaaaa aatccgaaa atatggcat 
      ttaagtgag gaacaatgg caccaagcc ggtttttcc 
      gcccggagt gcgacagtg acaccacgt cgagtgatt 
      ccggtccga aaatggaaa agtgatggt catcatgtt 
      tagggaact cgagtgaaa atgatgatg acacagaga 
      ggacctgga cctccgtat aatgtgatc ttttctctc 
      gttattttg gtcctagca tgcaccacg ttcgcagtc 
      ggcgaaaaa agcaaatat tattccatg cagtcgttg 
      tgcaagaac catttcctg cagcaactc taccggaaa 
      atcgacggt gccgttctg tggtcaaaa aacgagcga 
      aatttggat tgcgttatc acgtttcag acacactcg 
      attttgcag cggtttatg ctccggttc gactccttg 
      caacttgac tgcaacgac catctgtac atctatgat 
      ggggcgcat gccgtcggc acgtacaag tttgacctc 
      tcctgcaga aatacaaag caaacactg ggggccatg 
      ttcactcgc accaattac gttactctc aaatacgta 
      acggacgct tggggcacc aacgacaac ggctttacg 
      ctcgtcatc acggcagtg aaggacccg aagcatgcc 
      tgcactgag tttcgctgc aacttgcgt gagttttgt 
      atagccacg gacttggtc tgcgacagc atcaatcac 
      tgtgcggac ggctctgat gaatcttca aacacttta 
      tgtcagaat aacgaaacg ggaacaatc ctcggcttg 
      gaaatgacg tggtttgtt gtaacagta attagcact 
      ttgttagtt ttgttagtt tgcattgtt accgtttcc 
      atatgttta tgtcagcgc ggctggagc gccacgacc 
      agatccgga aacggaaac gtggcccaa ccgaacgcc 
      ccataccca cttcagcag atgtacggt tcgaatggt 
      gcgatgaaa cagggggct agcatgacg actttgaca 
      agtgctggc gccgccact gcaaccgcc gttcaggga 
      aactggcgc caccctgcg ggcccacta gggttccgc 
      ctcagcacg ccggcccgg gcccgcctc tactgccag 
      gcaaacagt agaagcggc cgagtcgta gcctgcagg 
      gtgagtcga gtgcagaac ccagcgctc ggccaacag 
      caccaatcg actgtcagc cacgtgacc tctcctcac 
      tgtgtgttt gactatcca aaggactgt atttacgtt 
      tagcaatgt ataagtata gtacgaaac agtgcattt 
      tacacagct ttctttcca aaactcgac acttaccta 
      actattctg tctggtatc atacctacc tacatatct 
      tagcgtcaa taaaaaaat tggtaaaaa tcttgcgag 
      gggaggcga tactaacat cacatattt cgtgtgaaa 
      gacaataca aaaacaaaa aattagcct aatctagag 
      ataatttag gatgcattc tgagagggg gtttgttac 
      ttgtgatgt cacacagct cagtactgc cgacaggag 
      gagaaagct taggaaacg tgtatcgaa tcaacgcac 
      acagccaat agacaaata cgaacgaca ctatagtac 
      tgtatgttt aactagata gatgtaatt agtggaaaa 
      agcactgta cttgtatag caccgtggc tgtcgaggt 
      cactggcag cctccttta gaacaataa agcgaattt 
      tcaatttgt ctatccaag aacgagtgt aaacttgta 
      tgtatagaa caataagct gctgctaag gcggtatta 
      gtgtaattt ttctatgga tgggacgga gcttggacc 
      cgatttttg gggcaacgg cgggtcaaa actccgtca 
      gcatcgcac gaacaactc caatgttat tctacatga 
      atttcgtac catttttta ctatagcag acctgacga 
      gtatgttta tacacatat cttttagac aagtaacta 
      gagtagctt caggacggc ttcgtttgt atattatcg 
      tagtacgct taccatagt cttgtgggc aaattttcc 
      tcttgccac tcatttact tcggttacc gcacggtaa 
      caactcgcc aaatacgtt tcacgaaac agaaacata 
      tcagggact cgcaatatc accgaaagc attttctaa 
      catatcaaa tttgctcac aagagattt atttttgtg 
      tacaatttg tccgctaca ataaaacgg tgcagatgt 
      ttagcaaaa aaccgatta tttaaaggc ctcattttc 
      gggcgcact ataaccacc acacacgat ttcatctcc 
      caaagtgtc attgcttca gccgcacac gtagttgca 
      attacgctg aaatcaccc tgaaaaatt tcagcgtaa 
      cttccgact tgactgatc acacccttc cctgtactt 
      acaccgttg accaatcgt tagaattac agtcctcta 
      gcaaattac caattaaaa tgtccgtgt gtgttcttt 
      aatggagta cagaaatgt ttctttaat aaaaaatct 
      gaaccaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY