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Gene information
General information
TC014762 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14765467..14771422
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014762
    iBeetle screen iB_09129

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014762 RA
      MSENSNSTS VMSEVTTLI RNWEDEHTA PNYDPIQTL 
      TRLAEICEV ETENYLKMD PDPFDERHP SRTDPDCKL 
      GQILKALFR KDIFMNKLV NDYLRETYY SRSGITDKD 
      VDQLNIAAC RLMLDVMPG LETSVVFQP DMDNLIHRL 
      IKWTTNSIE PLQSYATGL LAAAMEIPE IATRFREQN 
      GRLVPLLLQ RLRRLQNSS EFLTTTNTR PFAHLSSMK 
      SPPYRGDGV VKVSPSGIK RPRENGVIP NSKTTLNKE 
      NTDVINTSE LSDSESGQK RKKLKLEHN SSEADEGSP 
      QKPFLFNET SNSSWAELE SFMIGTVQI FPPTIATRQ 
      ILILRYLTP LGEYQEFLS HVFENDALS LVLKYVNVR 
      ATKEARLAF EALKYLAAL LCHKKFSIE FIQMDGLEA 
      LLEVPRPSI SATGVSICL YYLGYCEEA MERVCLLPK 
      YMITSLVKY ALWLLECSH DSGRCHAIM FFGLTFQFK 
      VILEEFDAH DGLRKLHNV ISTLSILLP DTDTTQLNE 
      DQECAARQI VRHVCVAYR RYLEAHLNL KVEPIKRSQ 
      LRLNEKMAQ PPLPAQPSY KPYKSSPEE VQQNVETLL 
      QYMPFRCHW TPVDQLLKL GGITLLLKI IAFAYEWNY 
      SGRAETVRS ALDTLAIAC VMPKVQLLF CDRVELPEE 
      SATVGINII LGAAEGEIV ADADVQKAA LRVIVNCVC 
      APINRVGGT LTRYSATVN SPIKKIKLK SSEELIQRV 
      WDCVRSNSG IMVLLQLMN IKTPITDAD CIRTLACKA 
      LAGLARSET VRQIVSKLP LFTSGQLQN LMRDPILQE 
      KRQEHVTFQ KYALELLER LSGKTKHRG NDLEVSLAN 
      IHRANVVAQ TKIQFNDRQ LLQLIHQHL ITKGCPETA 
      SMLVKEANL GNSVTPIST NQPTKFRYS STLTPSRLK 
      LNFTPMQHR TIPQSPVQK EETSFINSS NCTSPAIKL 
      IKKHQNQVV ATPPVQNSR LQKQTNSDV VVRVPSQSL 
      LDEPTNPPE QPRVTLDSI ITEYLTNQH ALCKNPMAN 
      CPKFNLFIP HKCPDPKPK IASANNFVV RCARRAIGY 
      QSKSMDRKF IHSRFCPVQ TIRSSSDGD FFTCAKFLP 
      GDKSIIVGD YSGDIRIFN LHTGNEEFM FQTHDNYIV 
      HLEPNRSGE FLLTSSTWG RPLSALWAI RNSEMKFAL 
      DDEEHVEFS KVTQERIVA TKLEVATIY DAVTGQSIL 
      RLVPTDSNQ YTRNKATFS PFDDLVLSD GVLFDVNSG 
      KQIHKLDKL NQTQSGVFH PNGLEIVSN TEVWDVRTF 
      HLLKTVPVL NQRTAVFSP VNSAIYAIS MEQELDNGD 
      STFDSSFKT VDAIDYSSI ATIDVKKNI YDLAVNRFD 
      TQIVIVENQ GVYQSVQES VVRIYDVGR KRDDEDEQD 
      EEDEEEEDD DMDGSDDDG SDNDDRLML NVGGNNDNN 
      DNNANNDDD NDEDDNFFD SESDSWTTT STGSDDSFT 
      DDMFDF
    CDS sequence
    • >TC014762 RA
      atgtcggaa aacagcaat tcgacttct gttatgtca 
      gaagtcaca actctgata agaaactgg gaagatgaa 
      catacggcg cctaattat gaccccatt cagacactc 
      accagattg gccgaaata tgcgaggtg gagactgaa 
      aattacctg aaaatggat cctgaccca tttgatgaa 
      cgccaccct tcacgaact gatcctgac tgcaaactt 
      ggccaaatt ttaaaagcg ctttttagg aaggatatt 
      tttatgaac aaacttgtt aacgattat ttgagagaa 
      acgtactac tctcggtca ggaatcaca gacaaggat 
      gttgatcag ttgaatatt gcagcttgt aggttgatg 
      ctagacgta atgcctggc ttagagaca tcagtggtg 
      tttcaaccc gatatggat aatttgatt caccgttta 
      attaaatgg actacaaat tcaatagaa cctctgcaa 
      agttacgca acggggctt ttagctgct gcaatggaa 
      attccagaa attgccaca cgttttagg gaacaaaac 
      ggacgactt gtgccttta ctacttcaa agactgcgt 
      aggcttcaa aattcctcg gaatttttg accacgaca 
      aatactcga ccttttgca cacttatct tcaatgaag 
      tcgccccct tatcgcggt gacggtgtc gtaaaagtt 
      tcaccctct ggtattaaa aggccccgc gagaatgga 
      gttattcca aattcgaaa acaacactt aataaagaa 
      aacactgat gtgattaac actagtgag ttatcagat 
      tcggaaagc ggccaaaaa cgcaaaaaa ctaaagctg 
      gagcacaat tcaagcgaa gctgatgaa ggctctcct 
      caaaaaccc ttcctattt aacgaaacg agcaattcg 
      tcgtgggca gagcttgaa agttttatg attggaacc 
      gtgcaaatt tttccgcca actatagca acgcgtcaa 
      atactcatt ttacgttat ttgacaccg ttgggtgaa 
      tatcaggag tttttgagt catgttttc gaaaatgac 
      gctcttagt ttggtctta aagtatgtg aatgtccga 
      gcgactaaa gaagcaaga cttgcattt gaagccttg 
      aaatatctt gctgctctt ttatgccac aaaaaattc 
      tcgattgag tttattcaa atggatggt cttgaggca 
      cttctggag gtgccaagg ccgtcaata tcagccact 
      ggggtctcg atttgtttg tactatttg gggtattgt 
      gaggaggca atggagagg gtttgtctg cttccgaaa 
      tacatgatc accagttta gtcaagtat gctttatgg 
      cttttggaa tgctcacac gattcgggc cgatgtcat 
      gctataatg tttttcggt ctcacgttt caattcaaa 
      gtgattttg gaggaattt gatgctcat gatgggtta 
      cgaaagtta cacaatgtg atttctact ttatcaatt 
      ttactgccg gacaccgat acgactcaa ctaaacgaa 
      gatcaagag tgcgccgct cgtcaaatc gttcgccac 
      gtttgtgtt gcataccgt cgttacctt gaagctcat 
      ctaaaccta aaagtagaa ccaattaaa agatcacaa 
      cttagactt aacgaaaaa atggcacag ccgccttta 
      ccggcgcag ccgagctac aaaccgtac aagtcatcg 
      cctgaagag gtgcaacag aacgtcgaa acgttgttg 
      caatatatg ccgtttcgg tgccattgg acgccagtt 
      gatcagtta ctcaaatta gggggaatc acactcttg 
      ttgaaaata atcgcgttt gcgtatgag tggaattat 
      agtgggagg gctgagacg gtcaggtcg gctttggat 
      acgttggct attgcgtgt gtgatgcct aaagttcaa 
      ttgttattt tgtgatagg gtggaattg ccagaggag 
      agtgccaca gttgggatt aatattatt ttgggggcc 
      gctgagggt gaaatagtg gccgatgct gatgtacaa 
      aaggctgcg cttcgagtc attgtcaat tgtgtgtgt 
      gcccccata aatagggtt ggtggtact ttaacccga 
      tattcggcc acggttaac agcccaatt aagaaaatt 
      aaactcaaa agtagcgaa gaattgatt cagagagtt 
      tgggattgt gtcaggtct aatagtggt attatggtt 
      ttgttgcaa ttaatgaat attaaaaca ccaatcact 
      gatgcggac tgtatacga actttggct tgtaaagca 
      ttggccggc ttagcacgg tcggaaact gtccgacag 
      atcgtgtca aaattaccg ctttttacg agcggacaa 
      ctgcagaat ttaatgaga gacccaatt ttgcaagaa 
      aaacgccaa gaacatgtg actttccaa aaatatgca 
      cttgaactt ttggaacga ctttcgggg aaaacgaaa 
      catcgggga aatgacttg gaagtttct ttagccaac 
      atacataga gctaacgtt gtggctcag actaaaatt 
      caattcaat gatcggcaa ttgttacaa ctcatccac 
      caacattta attacgaaa ggttgtcca gaaacggcc 
      tctatgtta gtgaaggaa gcaaatttg ggaaactca 
      gtcacgccc atttcaaca aatcaacca accaaattc 
      cgatactcg tccactcta acaccatct aggcttaag 
      ttaaatttc actccaatg caacaccgc acaataccg 
      cagtcacca gtgcaaaag gaggagaca tcgtttatt 
      aatagttcc aactgtact tcccctgct attaaatta 
      ataaagaaa caccaaaat caagtggtc gcgacacca 
      ccggtccaa aactcccga ttacagaaa caaacgaat 
      agcgatgtt gtagttagg gtgccgagt cagagcttg 
      cttgacgaa ccgacgaat ccacccgaa cagccccga 
      gtcacgctc gattcgatc ataaccgag tatttaacc 
      aatcagcat gcgttatgt aaaaacccc atggccaac 
      tgtcccaaa ttcaatctc tttattcca cataaatgc 
      ccagaccct aaaccaaag atagcctca gccaataat 
      tttgtggtg aggtgtgcg cgacgtgct attgggtat 
      cagtccaag tctatggac cggaaattt attcattcg 
      agattttgt ccagttcaa acgattcgg tcgtcgtcc 
      gatggcgat ttcttcact tgtgcgaaa tttttaccg 
      ggtgataaa agcataatc gtgggtgat tacagtggt 
      gatatacga atttttaat ctgcatact ggaaatgaa 
      gagtttatg tttcaaacg cacgataat tatatcgtt 
      catttggag cctaacagg agtggggag tttctgttg 
      acgagttcc acgtggggg cggcctctg tccgcactc 
      tgggccata aggaactcc gagatgaag tttgcgctg 
      gacgatgaa gaacatgtt gagtttagt aaagtgaca 
      caggagcgg attgtggcg actaaactt gaagttgca 
      acaatttac gacgccgtc acgggccaa tcaatcttg 
      cgtttagtt cctacagat agtaaccaa tacactaga 
      aataaagcc acatttagc ccgttcgat gatttggtt 
      ttatcagat ggtgtgctt ttcgatgtc aattcgggc 
      aaacagatt cacaagttg gataagttg aatcagacc 
      caaagtggg gtgttccac cctaacggc ttagagatt 
      gtatcaaat actgaggtt tgggatgtg cgaactttt 
      catttgttg aaaacagtg ccagttttg aatcaacgt 
      actgccgtt ttttcaccg gtcaatagt gcgatttat 
      gccatttcg atggaacag gaattggat aatggtgac 
      tcgacattc gattcgagt tttaaaaca gtggatgcg 
      atcgattac tcgagtatt gctactatt gacgttaag 
      aaaaacatc tacgatttg gcggttaat cggttcgat 
      acgcaaatt gtgattgtg gagaatcag ggggtttac 
      cagtcggtt caagagtcc gtggtgcgg atctatgat 
      gtcgggagg aagcgagac gatgaggat gaacaagat 
      gaagaggac gaggaagag gaggatgat gatatggat 
      gggagtgac gatgacggt tccgataat gatgatcga 
      ttgatgctc aacgtgggc gggaataac gacaacaac 
      gataataac gccaacaat gatgatgac aatgatgag 
      gacgataat ttcttcgat agtgagtct gatagttgg 
      acgactacg tcgactggc tctgatgat tcgttcacg 
      gatgatatg ttcgatttt tga
    mRNA sequence
    • >TC014762 RA
      ctctgtttt ttttttgta ccattggcg gcacttttt 
      tagtaacat acgtgctcg atttttcaa tggaagcca 
      agaattgtg ttttgcgct ttgtgaatg tgtattgcg 
      ccaatttgt gaaataatc aaactgatt acgattttt 
      tacaagcca gaagtgtag agttcacat tatcgcatt 
      gtttttgcc agtctcccg gtgtcctaa ttatctcac 
      cttgcaatt acggaactg taaagtatt ttaggttat 
      gttaaacta cctcatgcc ctttatttt ataacgttt 
      tgagccaat aatgtcgga aaacagcaa ttcgacttc 
      tgttatgtc agaagtcac aactctgat aagaaactg 
      ggaagatga acatacggc gcctaatta tgaccccat 
      tcagacact caccagatt ggccgaaat atgcgaggt 
      ggagactga aaattacct gaaaatgga tcctgaccc 
      atttgatga acgccaccc ttcacgaac tgatcctga 
      ctgcaaact tggccaaat tttaaaagc gctttttag 
      gaaggatat ttttatgaa caaacttgt taacgatta 
      tttgagaga aacgtacta ctctcggtc aggaatcac 
      agacaagga tgttgatca gttgaatat tgcagcttg 
      taggttgat gctagacgt aatgcctgg cttagagac 
      atcagtggt gtttcaacc cgatatgga taatttgat 
      tcaccgttt aattaaatg gactacaaa ttcaataga 
      acctctgca aagttacgc aacggggct tttagctgc 
      tgcaatgga aattccaga aattgccac acgttttag 
      ggaacaaaa cggacgact tgtgccttt actacttca 
      aagactgcg taggcttca aaattcctc ggaattttt 
      gaccacgac aaatactcg accttttgc acacttatc 
      ttcaatgaa gtcgccccc ttatcgcgg tgacggtgt 
      cgtaaaagt ttcaccctc tggtattaa aaggccccg 
      cgagaatgg agttattcc aaattcgaa aacaacact 
      taataaaga aaacactga tgtgattaa cactagtga 
      gttatcaga ttcggaaag cggccaaaa acgcaaaaa 
      actaaagct ggagcacaa ttcaagcga agctgatga 
      aggctctcc tcaaaaacc cttcctatt taacgaaac 
      gagcaattc gtcgtgggc agagcttga aagttttat 
      gattggaac cgtgcaaat ttttccgcc aactatagc 
      aacgcgtca aatactcat tttacgtta tttgacacc 
      gttgggtga atatcagga gtttttgag tcatgtttt 
      cgaaaatga cgctcttag tttggtctt aaagtatgt 
      gaatgtccg agcgactaa agaagcaag acttgcatt 
      tgaagcctt gaaatatct tgctgctct tttatgcca 
      caaaaaatt ctcgattga gtttattca aatggatgg 
      tcttgaggc acttctgga ggtgccaag gccgtcaat 
      atcagccac tggggtctc gatttgttt gtactattt 
      ggggtattg tgaggaggc aatggagag ggtttgtct 
      gcttccgaa atacatgat caccagttt agtcaagta 
      tgctttatg gcttttgga atgctcaca cgattcggg 
      ccgatgtca tgctataat gtttttcgg tctcacgtt 
      tcaattcaa agtgatttt ggaggaatt tgatgctca 
      tgatgggtt acgaaagtt acacaatgt gatttctac 
      tttatcaat tttactgcc ggacaccga tacgactca 
      actaaacga agatcaaga gtgcgccgc tcgtcaaat 
      cgttcgcca cgtttgtgt tgcataccg tcgttacct 
      tgaagctca tctaaacct aaaagtaga accaattaa 
      aagatcaca acttagact taacgaaaa aatggcaca 
      gccgccttt accggcgca gccgagcta caaaccgta 
      caagtcatc gcctgaaga ggtgcaaca gaacgtcga 
      aacgttgtt gcaatatat gccgtttcg gtgccattg 
      gacgccagt tgatcagtt actcaaatt agggggaat 
      cacactctt gttgaaaat aatcgcgtt tgcgtatga 
      gtggaatta tagtgggag ggctgagac ggtcaggtc 
      ggctttgga tacgttggc tattgcgtg tgtgatgcc 
      taaagttca attgttatt ttgtgatag ggtggaatt 
      gccagagga gagtgccac agttgggat taatattat 
      tttgggggc cgctgaggg tgaaatagt ggccgatgc 
      tgatgtaca aaaggctgc gcttcgagt cattgtcaa 
      ttgtgtgtg tgcccccat aaatagggt tggtggtac 
      tttaacccg atattcggc cacggttaa cagcccaat 
      taagaaaat taaactcaa aagtagcga agaattgat 
      tcagagagt ttgggattg tgtcaggtc taatagtgg 
      tattatggt tttgttgca attaatgaa tattaaaac 
      accaatcac tgatgcgga ctgtatacg aactttggc 
      ttgtaaagc attggccgg cttagcacg gtcggaaac 
      tgtccgaca gatcgtgtc aaaattacc gctttttac 
      gagcggaca actgcagaa tttaatgag agacccaat 
      tttgcaaga aaaacgcca agaacatgt gactttcca 
      aaaatatgc acttgaact tttggaacg actttcggg 
      gaaaacgaa acatcgggg aaatgactt ggaagtttc 
      tttagccaa catacatag agctaacgt tgtggctca 
      gactaaaat tcaattcaa tgatcggca attgttaca 
      actcatcca ccaacattt aattacgaa aggttgtcc 
      agaaacggc ctctatgtt agtgaagga agcaaattt 
      gggaaactc agtcacgcc catttcaac aaatcaacc 
      aaccaaatt ccgatactc gtccactct aacaccatc 
      taggcttaa gttaaattt cactccaat gcaacaccg 
      cacaatacc gcagtcacc agtgcaaaa ggaggagac 
      atcgtttat taatagttc caactgtac ttcccctgc 
      tattaaatt aataaagaa acaccaaaa tcaagtggt 
      cgcgacacc accggtcca aaactcccg attacagaa 
      acaaacgaa tagcgatgt tgtagttag ggtgccgag 
      tcagagctt gcttgacga accgacgaa tccacccga 
      acagccccg agtcacgct cgattcgat cataaccga 
      gtatttaac caatcagca tgcgttatg taaaaaccc 
      catggccaa ctgtcccaa attcaatct ctttattcc 
      acataaatg cccagaccc taaaccaaa gatagcctc 
      agccaataa ttttgtggt gaggtgtgc gcgacgtgc 
      tattgggta tcagtccaa gtctatgga ccggaaatt 
      tattcattc gagattttg tccagttca aacgattcg 
      gtcgtcgtc cgatggcga tttcttcac ttgtgcgaa 
      atttttacc gggtgataa aagcataat cgtgggtga 
      ttacagtgg tgatatacg aatttttaa tctgcatac 
      tggaaatga agagtttat gtttcaaac gcacgataa 
      ttatatcgt tcatttgga gcctaacag gagtgggga 
      gtttctgtt gacgagttc cacgtgggg gcggcctct 
      gtccgcact ctgggccat aaggaactc cgagatgaa 
      gtttgcgct ggacgatga agaacatgt tgagtttag 
      taaagtgac acaggagcg gattgtggc gactaaact 
      tgaagttgc aacaattta cgacgccgt cacgggcca 
      atcaatctt gcgtttagt tcctacaga tagtaacca 
      atacactag aaataaagc cacatttag cccgttcga 
      tgatttggt tttatcaga tggtgtgct tttcgatgt 
      caattcggg caaacagat tcacaagtt ggataagtt 
      gaatcagac ccaaagtgg ggtgttcca ccctaacgg 
      cttagagat tgtatcaaa tactgaggt ttgggatgt 
      gcgaacttt tcatttgtt gaaaacagt gccagtttt 
      gaatcaacg tactgccgt tttttcacc ggtcaatag 
      tgcgattta tgccatttc gatggaaca ggaattgga 
      taatggtga ctcgacatt cgattcgag ttttaaaac 
      agtggatgc gatcgatta ctcgagtat tgctactat 
      tgacgttaa gaaaaacat ctacgattt ggcggttaa 
      tcggttcga tacgcaaat tgtgattgt ggagaatca 
      gggggttta ccagtcggt tcaagagtc cgtggtgcg 
      gatctatga tgtcgggag gaagcgaga cgatgagga 
      tgaacaaga tgaagagga cgaggaaga ggaggatga 
      tgatatgga tgggagtga cgatgacgg ttccgataa 
      tgatgatcg attgatgct caacgtggg cgggaataa 
      cgacaacaa cgataataa cgccaacaa tgatgatga 
      caatgatga ggacgataa tttcttcga tagtgagtc 
      tgatagttg gacgactac gtcgactgg ctctgatga 
      ttcgttcac ggatgatat gttcgattt ttgagcgaa 
      ccgtaaaat aaggtttaa atgtgatag caattcgaa 
      aatagttaa ttggatgag tgggcgtgt gtctgtttt 
      ttataagga aaacggcat ttgatgatt ttccagctt 
      gtggtgtca agtgtgttt ttaaatgtg agccgagtt 
      tggaattat gttgaacat tgtgtatta tatttcata 
      aataaatac aatttcaaa gg
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