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Gene information
General information
TC014758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14728927..14731200
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014758
    iBeetle screen iB_09127

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014758 RA
      MPKVRSTGG PPRKQGFNK SNHSMNPER LKEGLKGVA 
      KVRSKSTIK RLQMYRNFK AKRDKTGKI LTPAPFQGR 
      LPSGTQARV EPNQKWFGN SRVISQNAL QKFQTELGA 
      AVKNPYQMV MKPTTLPIT LLNEKAKNA RVHLLDTES 
      FDSVFGPKK SRKRPNLAV KGLDELSKL IEEKHEKYS 
      NEKDSNIVR DDGGVRDLP RDWVMAAGQ SKRIWNELY 
      KVVDSSDVL LQVLDARDP MGTRSPYLE KFLKTEKPH 
      KHLIFILNK VDLVPTWAT QRWVAILSK EYPTIAFHA 
      SIMHPFGKG ALINLLRQF AKLHIDKKQ ISVGFIGYP 
      NVGKSSIIN TLRSKKVCK VAPIAGETK VWQYITLMR 
      RIYLIDCPG VVYPSAETD TEKVLKGVV RVELVNNPE 
      DYIEAVLER VRKEYLVKT YKIEDWTSA TDFLEKLAQ 
      RSGKLLKKG EPDIGIVAR MVLNDWQRG KLPFYVSPP 
      GFEEPLPTT EQDNELSVV QDYNKIRVG LQYEGEDVK 
      ELEPIKDIE KAVVENEDK EDDEKEEED NIANNSEVK 
      DTDDAGTES DDSSDLSNI YSDMEDSKE TEYAVSASG 
      PFTVENIAS KKQKKGRKV EDMALGGKK LTSKQRRAI 
      ERAQKRRKI GSNFYEVAN VKNRSYRPK HKKNSSV
    CDS sequence
    • >TC014758 RA
      atgcccaaa gtgcgttcg acggggggc cctccccga 
      aaacagggc tttaacaag tcgaaccac tcgatgaat 
      cccgagcgc ctcaaagag gggctcaag ggggtggcc 
      aaagtgcgc tcaaaatcg accatcaag cggctgcaa 
      atgtacaga aactttaaa gcaaagcgg gacaaaact 
      ggaaaaatt ttaacccca gccccgttc cagggccgt 
      ttgccgtcc gggacccaa gctagggtc gaaccgaac 
      cagaagtgg tttggcaac agtcgggtc atttcacaa 
      aacgcccta caaaagttc caaaccgag ttgggcgcc 
      gctgtaaaa aatccctat cagatggtt atgaaacca 
      accactttg cccatcact ttgttaaac gagaaggcg 
      aaaaatgca agagttcac ttgttggat accgagagt 
      tttgatagt gttttcggg ccgaaaaaa agcaggaaa 
      cggccgaat ctggcggtg aagggtttg gacgagttg 
      tcgaaatta attgaagaa aaacacgag aagtacagt 
      aatgagaaa gactcaaat attgtcagg gatgacgga 
      ggtgttcgg gacttgccc agggattgg gtcatggca 
      gcaggacag tctaaacga atttggaac gaattgtac 
      aaagttgtg gatagttca gacgttttg ctccaagta 
      ctagatgcc agggacccg atggggaca aggtccccc 
      tatttagaa aaattttta aagacggaa aagccccac 
      aaacacctg atttttatt ttgaataaa gttgactta 
      gtcccaact tgggccact caaaggtgg gttgctatt 
      ttaagtaag gagtacccc acaattgcc tttcatgcc 
      agcataatg caccccttt ggcaaaggg gccctgatt 
      aacctcctg aggcaattt gctaaactg cacattgac 
      aagaagcaa atctctgtc ggatttatt ggctaccca 
      aatgttggc aagtcctca attatcaac acactaaga 
      agtaaaaaa gtgtgtaaa gtggcccca atagctggc 
      gagactaaa gtctggcaa tacatcact ctaatgagg 
      cgaatttac ctgattgat tgtcccgga gttgtttat 
      ccaagcgct gaaactgat actgaaaaa gtgctgaaa 
      ggagttgtt agagtagaa cttgtaaat aatccagaa 
      gattatatt gaggcagtt ttggaacgc gttagaaag 
      gaatatttg gtcaaaact tacaaaatt gaggactgg 
      actagtgcc actgatttt ttggaaaag ttggcacaa 
      aggtctgga aaattactg aaaaagggg gagcctgat 
      attggaatt gttgcaagg atggtttta aatgattgg 
      cagagaggg aaattgcca ttttatgtt tctcctccc 
      ggatttgag gaacctttg cctacgaca gagcaagac 
      aatgaattg agtgttgta caggattat aataaaatc 
      agagttggc ttgcaatac gagggtgaa gatgtgaaa 
      gaattggaa ccaatcaaa gacattgag aaggctgta 
      gttgaaaat gaagataag gaagatgac gaaaaggaa 
      gaagaagac aacattgcc aacaatagc gaagttaag 
      gacactgac gatgctgga acagaatct gatgactcg 
      tcagatttg agtaacatt tactctgac atggaagat 
      tccaaagaa accgaatat gccgtatca gcaagtggg 
      ccgtttact gttgaaaac attgcaagt aaaaagcag 
      aaaaagggg cgcaaagtt gaagacatg gctcttgga 
      ggcaagaaa ttaacaagc aagcaacgg cgggcgata 
      gaaagggcg caaaagcgg aggaaaatc ggcagcaac 
      ttctacgaa gttgcgaat gttaagaat aggagttac 
      agaccgaaa cataaaaaa aattcaagt gtttag
    mRNA sequence
    • >TC014758 RA
      aagacccca agtcataat attcagtac aattagacg 
      aacccaacc acaacccat tccaatttt tcttattcc 
      taatttcaa gaatcgacg agatttttt gtagttgca 
      caacctgac attcaaact tgacagtat taatgacag 
      cacaaacac gtgtagttc aatttggaa attttgaat 
      aaactcgga ggtgatttg tatcacaat gcccaaagt 
      gcgttcgac ggggggccc tccccgaaa acagggctt 
      taacaagtc gaaccactc gatgaatcc cgagcgcct 
      caaagaggg gctcaaggg ggtggccaa agtgcgctc 
      aaaatcgac catcaagcg gctgcaaat gtacagaaa 
      ctttaaagc aaagcggga caaaactgg aaaaatttt 
      aaccccagc cccgttcca gggccgttt gccgtccgg 
      gacccaagc tagggtcga accgaacca gaagtggtt 
      tggcaacag tcgggtcat ttcacaaaa cgccctaca 
      aaagttcca aaccgagtt gggcgccgc tgtaaaaaa 
      tccctatca gatggttat gaaaccaac cactttgcc 
      catcacttt gttaaacga gaaggcgaa aaatgcaag 
      agttcactt gttggatac cgagagttt tgatagtgt 
      tttcgggcc gaaaaaaag caggaaacg gccgaatct 
      ggcggtgaa gggtttgga cgagttgtc gaaattaat 
      tgaagaaaa acacgagaa gtacagtaa tgagaaaga 
      ctcaaatat tgtcaggga tgacggagg tgttcggga 
      cttgcccag ggattgggt catggcagc aggacagtc 
      taaacgaat ttggaacga attgtacaa agttgtgga 
      tagttcaga cgttttgct ccaagtact agatgccag 
      ggacccgat ggggacaag gtcccccta tttagaaaa 
      atttttaaa gacggaaaa gccccacaa acacctgat 
      ttttatttt gaataaagt tgacttagt cccaacttg 
      ggccactca aaggtgggt tgctatttt aagtaagga 
      gtaccccac aattgcctt tcatgccag cataatgca 
      cccctttgg caaaggggc cctgattaa cctcctgag 
      gcaatttgc taaactgca cattgacaa gaagcaaat 
      ctctgtcgg atttattgg ctacccaaa tgttggcaa 
      gtcctcaat tatcaacac actaagaag taaaaaagt 
      gtgtaaagt ggccccaat agctggcga gactaaagt 
      ctggcaata catcactct aatgaggcg aatttacct 
      gattgattg tcccggagt tgtttatcc aagcgctga 
      aactgatac tgaaaaagt gctgaaagg agttgttag 
      agtagaact tgtaaataa tccagaaga ttatattga 
      ggcagtttt ggaacgcgt tagaaagga atatttggt 
      caaaactta caaaattga ggactggac tagtgccac 
      tgatttttt ggaaaagtt ggcacaaag gtctggaaa 
      attactgaa aaaggggga gcctgatat tggaattgt 
      tgcaaggat ggttttaaa tgattggca gagagggaa 
      attgccatt ttatgtttc tcctcccgg atttgagga 
      acctttgcc tacgacaga gcaagacaa tgaattgag 
      tgttgtaca ggattataa taaaatcag agttggctt 
      gcaatacga gggtgaaga tgtgaaaga attggaacc 
      aatcaaaga cattgagaa ggctgtagt tgaaaatga 
      agataagga agatgacga aaaggaaga agaagacaa 
      cattgccaa caatagcga agttaagga cactgacga 
      tgctggaac agaatctga tgactcgtc agatttgag 
      taacattta ctctgacat ggaagattc caaagaaac 
      cgaatatgc cgtatcagc aagtgggcc gtttactgt 
      tgaaaacat tgcaagtaa aaagcagaa aaaggggcg 
      caaagttga agacatggc tcttggagg caagaaatt 
      aacaagcaa gcaacggcg ggcgataga aagggcgca 
      aaagcggag gaaaatcgg cagcaactt ctacgaagt 
      tgcgaatgt taagaatag gagttacag accgaaaca 
      taaaaaaaa ttcaagtgt ttaggcttt atttaaatt 
      tttaataaa cagttttcc gaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY