Status
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Gene information
General information
TC014728 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14134669..14162199
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014728
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09125

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 3

    vitellogenic egg chamber not present - Number of animals showing the phenotype: 3
    previtellogenic egg chamber position irregular - Number of animals showing the phenotype: 3

    previtellogenic egg chamber early amount increased - Number of animals showing the phenotype: 3
    previtellogenic egg chamber late not present - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014728 RA
      MHANVHTDR NEWRLPPDE TLQVADPKA KKAEELALH 
      EDEGHNWWS IIFSLLVIG LVISGIVAA IFLVGYVDE 
      LLYWHGTRM QFDEFLQGE LTPKRLPSS WISHSHFVY 
      QTDDGSLAI LDTSHNFTV SILVTNHTL RQLLVKGYQ 
      CSKDLKYVL FQHDVKSVF RQSFTATYT IYDVSKDHQ 
      IPLRLEMSS KAEPETFQY VSWLGDTTS LLMVYNNDI 
      YFRQSPMDE TETRLTFSG RPEVIYNGI PDWLYQEDV 
      LKSPQAMWS SHDGTHLLY ATFNDSEVG ILNFPWFRT 
      EAGLGASSP AASFPSSRS VRFPRPGAA NPTVQLWVM 
      DISNLTDIQ SHLVKPPQA LVGQDYYLA SAGWIGQKN 
      TQVSVVWMN RAQNLSIIS ACLAPNWTC VEIHAERAA 
      ERAWLDIQE HPIFSADGD SFLMLAAVQ EGSREAYTH 
      IKHVTVTQQ HIAVLSHGR HEVDRILSW DNASHLIYY 
      LATEERKAG QRHLYVVRD PSTDDPRRL ESHCITCEI 
      GDVLWSSRY LYGNCTHFN ALISPRSES SNSSFYVLQ 
      CEGPGLPLA GMHNATSHK LLKILYDTR LQKMGMLER 
      LALPKQKSF EVPLPHGSR AQVQLLLPP SWREELRDA 
      AYPVLVEVN GRPGSKAVT EKFRIDWGT YMSSHCDVV 
      YIRLDVRGA SGQSDRALF RHLGGVEVQ DQVAVLELL 
      LNTYKYLDK TRVGIWGWG YGGYVTSMV LGLGNQQKV 
      YKCGAAVSP ISDWAYYNS AFTEKILGD PNENKKGYV 
      QADATQRAQ NIPRNSFYL LHGMADLTA PYIHGVSLA 
      RALTEAGVL FRYQSYANE GHELENVLE HVYSSMQSF 
      FEECLRLDV GEPANDKDT
    CDS sequence
    • >TC014728 RA
      atgcacgct aatgtgcac acagaccgg aacgaatgg 
      cgactgccc ccggatgag actttgcag gtcgccgat 
      ccaaaggcc aaaaaggcc gaggagcta gcattacac 
      gaagatgag ggacacaat tggtggtcg attattttt 
      tcgttactg gttattgga ctcgttatc tccggaatt 
      gtagctgct attttcctt gtggggtat gttgatgaa 
      cttctttac tggcatggg acgaggatg caatttgat 
      gagttcttg cagggcgag ctgactccc aaacgatta 
      ccctcttcc tggatttcg cactctcat ttcgtttac 
      caaacggac gacggaagt ctggccata ctcgacact 
      agtcataat ttcactgtt tcgatccta gttactaac 
      cacactttg agacaactc ctcgtcaaa ggataccag 
      tgctcaaag gaccttaaa tatgtgctc ttccaacac 
      gacgtaaaa tcagtattc agacaatcc ttcacggcg 
      acgtacacc atctatgac gttagtaaa gatcatcaa 
      atcccactc cggttggaa atgtcgtcg aaggccgag 
      cccgagacg ttccaatac gttagctgg ttgggcgac 
      acaacttcg ctcttaatg gtgtataat aacgacata 
      tatttccgg cagtctccc atggacgag accgagacc 
      cgcctgacg ttttcgggg cgtcccgag gtgatctat 
      aatggaatt ccagattgg ctctaccaa gaggacgtg 
      ctcaagagc ccgcaagcg atgtggagc tcgcacgat 
      ggcactcac ctgttgtac gcaacgttc aacgacagc 
      gaagtggga atattaaat ttcccgtgg tttcgaacg 
      gaagccggc ttaggggcc tccagcccc gctgcgtcc 
      ttcccgtcc tcgcgcagc gttcgattt ccgaggccc 
      ggggcggcc aaccccacg gtgcagctg tgggtcatg 
      gacatctcg aacctcacc gacatccag agccacttg 
      gtaaaaccc ccgcaagcg ctcgtcggc caggattac 
      tatttggcg tcggctggc tggattgga cagaagaac 
      acgcaagtg tcggtggtg tggatgaac cgcgcacaa 
      aacctcagc atcatttcg gcctgtcta gcgcccaac 
      tggacatgt gtagagatt cacgctgaa cgcgcagca 
      gagagggct tggctcgac atccaggag catccgatc 
      ttttcagcg gatggcgac agtttcctg atgcttgct 
      gctgtccag gaggggagc agggaagct tacacgcat 
      attaagcac gtgactgtc actcaacag cacatcgct 
      gtcctcagc cacggtcga cacgaggtc gatcgaatc 
      ttgagctgg gataatgcg agccatctg atctattac 
      ttggcgacg gaggagcgc aaggcgggt caaaggcat 
      ctgtacgta gtacgtgac ccgtctaca gacgacccc 
      cgacggctt gaatcccac tgcataact tgcgagatc 
      ggggacgtc ctctggagc agtcgttat ctttacggc 
      aactgtacg cacttcaac gctttgata agccctcgt 
      tcggaatca tcgaattcg tcattttat gtccttcaa 
      tgcgaggga cccggtctt ccattagca ggcatgcat 
      aatgcgact tcgcacaag ctattaaag attctttac 
      gatacgagg ctgcagaaa atgggaatg ctcgagagg 
      ttagcgttg cccaagcaa aagtctttc gaggtgcct 
      ttgccccat ggtagtagg gctcaagtt caactactt 
      ctacctccc agctggagg gaggaactg cgagacgct 
      gcctaccct gtccttgtt gaagttaac ggcagaccg 
      ggcagcaaa gcagtgacc gaaaaattt aggatagat 
      tggggtacc tacatgtcg agccactgc gacgtggtt 
      tacatccgc ctggacgtg cggggggcc agtggccag 
      tcggaccgc gcccttttc cgccacttg gggggcgtg 
      gaagtgcag gaccaagtg gcggtgctg gaactcctc 
      ctcaacacg tacaaatac ttggacaaa actcgcgtc 
      ggaatctgg ggttggggc tacggcggg tacgtcacc 
      tccatggtg ctggggctg ggcaaccag cagaaagtg 
      tacaagtgt ggggcggcg gtgagcccc atctccgac 
      tgggcgtat tacaattca gcattcacg gagaagatc 
      ctgggggac cccaacgag aacaagaag gggtacgtg 
      caggcggac gccacgcag cgcgcgcag aacatcccc 
      cggaactcc ttttacctg ctgcacggt atggctgac 
      ctcacggct ccgtacatc cacggggtt tccctggca 
      cgtgccctg acggaagcc ggggtcctg ttccgatat 
      cagagttac gccaacgag ggtcacgaa ctcgaaaac 
      gttctggaa cacgtgtac agctcgatg caaagcttc 
      ttcgaagag tgccttaga ctagatgta ggcgagcca 
      gctaatgat aaagatacc tga
    mRNA sequence
    • >TC014728 RA
      tcggccgga acccgaaca tgatgcaca gcgtcgcga 
      cgcctaaac tcggcacaa gtctacctg tagtggcag 
      tgcttccta cacaccgtt atgcctatt ggtgatagt 
      ttgcagtca cttcttcag tcaagaaac cacgctctg 
      tggccgatt tcccacagt cttgccctt cagcttatg 
      agaccgcga ccggagtct gaaacagac cgaaactgt 
      tgaatctac tcgcaagag ccgacttcc agaagagga 
      ggtgacgcc gagttggga cagtggcgg cacagaagg 
      gtgagaaca cggggcgga aagcggaat gcacgctaa 
      tgtgcacac agaccggaa cgaatggcg actgccccc 
      ggatgagac tttgcaggt cgccgatcc aaaggccaa 
      aaaggccga ggagctagc attacacga agatgaggg 
      acacaattg gtggtcgat tattttttc gttactggt 
      tattggact cgttatctc cggaattgt agctgctat 
      tttccttgt ggggtatgt tgatgaact tctttactg 
      gcatgggac gaggatgca atttgatga gttcttgca 
      gggcgagct gactcccaa acgattacc ctcttcctg 
      gatttcgca ctctcattt cgtttacca aacggacga 
      cggaagtct ggccatact cgacactag tcataattt 
      cactgtttc gatcctagt tactaacca cactttgag 
      acaactcct cgtcaaagg ataccagtg ctcaaagga 
      ccttaaata tgtgctctt ccaacacga cgtaaaatc 
      agtattcag acaatcctt cacggcgac gtacaccat 
      ctatgacgt tagtaaaga tcatcaaat cccactccg 
      gttggaaat gtcgtcgaa ggccgagcc cgagacgtt 
      ccaatacgt tagctggtt gggcgacac aacttcgct 
      cttaatggt gtataataa cgacatata tttccggca 
      gtctcccat ggacgagac cgagacccg cctgacgtt 
      ttcggggcg tcccgaggt gatctataa tggaattcc 
      agattggct ctaccaaga ggacgtgct caagagccc 
      gcaagcgat gtggagctc gcacgatgg cactcacct 
      gttgtacgc aacgttcaa cgacagcga agtgggaat 
      attaaattt cccgtggtt tcgaacgga agccggctt 
      aggggcctc cagccccgc tgcgtcctt cccgtcctc 
      gcgcagcgt tcgatttcc gaggcccgg ggcggccaa 
      ccccacggt gcagctgtg ggtcatgga catctcgaa 
      cctcaccga catccagag ccacttggt aaaaccccc 
      gcaagcgct cgtcggcca ggattacta tttggcgtc 
      ggctggctg gattggaca gaagaacac gcaagtgtc 
      ggtggtgtg gatgaaccg cgcacaaaa cctcagcat 
      catttcggc ctgtctagc gcccaactg gacatgtgt 
      agagattca cgctgaacg cgcagcaga gagggcttg 
      gctcgacat ccaggagca tccgatctt ttcagcgga 
      tggcgacag tttcctgat gcttgctgc tgtccagga 
      ggggagcag ggaagctta cacgcatat taagcacgt 
      gactgtcac tcaacagca catcgctgt cctcagcca 
      cggtcgaca cgaggtcga tcgaatctt gagctggga 
      taatgcgag ccatctgat ctattactt ggcgacgga 
      ggagcgcaa ggcgggtca aaggcatct gtacgtagt 
      acgtgaccc gtctacaga cgacccccg acggcttga 
      atcccactg cataacttg cgagatcgg ggacgtcct 
      ctggagcag tcgttatct ttacggcaa ctgtacgca 
      cttcaacgc tttgataag ccctcgttc ggaatcatc 
      gaattcgtc attttatgt ccttcaatg cgagggacc 
      cggtcttcc attagcagg catgcataa tgcgacttc 
      gcacaagct attaaagat tctttacga tacgaggct 
      gcagaaaat gggaatgct cgagaggtt agcgttgcc 
      caagcaaaa gtctttcga ggtgccttt gccccatgg 
      tagtagggc tcaagttca actacttct acctcccag 
      ctggaggga ggaactgcg agacgctgc ctaccctgt 
      ccttgttga agttaacgg cagaccggg cagcaaagc 
      agtgaccga aaaatttag gatagattg gggtaccta 
      catgtcgag ccactgcga cgtggttta catccgcct 
      ggacgtgcg gggggccag tggccagtc ggaccgcgc 
      ccttttccg ccacttggg gggcgtgga agtgcagga 
      ccaagtggc ggtgctgga actcctcct caacacgta 
      caaatactt ggacaaaac tcgcgtcgg aatctgggg 
      ttggggcta cggcgggta cgtcacctc catggtgct 
      ggggctggg caaccagca gaaagtgta caagtgtgg 
      ggcggcggt gagccccat ctccgactg ggcgtatta 
      caattcagc attcacgga gaagatcct gggggaccc 
      caacgagaa caagaaggg gtacgtgca ggcggacgc 
      cacgcagcg cgcgcagaa catcccccg gaactcctt 
      ttacctgct gcacggtat ggctgacct cacggctcc 
      gtacatcca cggggtttc cctggcacg tgccctgac 
      ggaagccgg ggtcctgtt ccgatatca gagttacgc 
      caacgaggg tcacgaact cgaaaacgt tctggaaca 
      cgtgtacag ctcgatgca aagcttctt cgaagagtg 
      ccttagact agatgtagg cgagccagc taatgataa 
      agatacctg aagattggt ttcccactt acttcacca 
      ccacgtgac ggccttcga aacgcagac attccacgt 
      gttatcagt tacgaaaag aagaaaaca gcagaaacc 
      gactaacgg aactagtca attcaatca ataatcaag 
      ctgtttata tcatagcaa aacttagtg agttgttca 
      gtgtagaac gacgagacg aattagtga ttccttttc 
      tcaaatttt cttgttgag cggttggac tgatatcag 
      attttatat attagatcc aagttgtga cctttcctc 
      ttcttacac cgatttcca aactacttt taatgacga 
      gtttttaca gtgaaaagt agttaatgt cattagatc 
      tcaacccaa aagccgaat atcacacga ttaccaaaa 
      caaacattc ataaagtat taacctagt atcgtaaga 
      actaatttt aaataccgt tagtattga aattcttcc 
      atagaagta gactattgt aaatagctt gaattgatt 
      agaataaga ctgatttta taaattatc aaaaaacgc 
      ttaagcttt cgattgttt gatacaaac aaacgctgt 
      tttaatctt cacgtatct caagttaga caaatttaa 
      cggtggcgt ccaagcctg taaatgaaa accactggt 
      taaaaccgc acgtttcat ttttgatat cgacagatc 
      cacagcaaa atctaatca aagacaatc tcaagagca 
      aaattttta ttgatgaca ttaaatact ttccactca 
      gctgtcggt tcacacttt tctatgtaa ctgtttctt 
      ggtttccct cttctagca aagtaatag tacagatga 
      tgtcaatta ttgaaaatg tttttaaat tttcggaaa 
      gctgaatta cgacgataa atcaaaata aaacgactt 
      aattattaa actggagct tcgatattt aaaagtaat 
      caataaaat ggttgtgaa atataaaac tttccattt 
      aaacaagaa tatattaca tagtgtaaa ttttgctta 
      gatttatta gtagaagct gtgcttctg tcacagcga 
      agacaatct ttacgagct gatattatg tccgaattg 
      gttttctcg tgtccacta acgctgtca aattttcgg 
      aatcatcga cctcaacat tctaaattc ggtggtcta 
      atttaaaac ttttggcct atttctcag aggatttgt 
      gtttctaac gacgattat ctcgatgtg ttattttta 
      tagcaatac taatttgtt gcaaatgtt gcctaataa 
      ttgttgtac acatcagtg tttgtaaat taaaataat 
      gtgcagaac accaattaa attattgat ttttattag 
      acagcaatg ttagataat ggaatgtta aatgtttct 
      tttttgtat tatattaaa aacaaaatg atgcaaaca 
      atgtttaca ataaatcgt tattaagta c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY