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Gene information
General information
TC014648 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12817817..12822468
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014648
    iBeetle screen iB_09122

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber sometimes number decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber potentially degenerated - Number of animals showing the phenotype: 4

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014648 RA
      MFEGLLTVR YVTSKNCFC VLSVNNFKH LQNNVLKLS 
      YNDNEAIHV SVDPLSGSV DDQSLGINS LYAKALGLE 
      DNSLVTVSE ISKPPTIQS VTITAVDSN DHLVLESLA 
      ENVQSTLLE QIRVLTNGQ KFVIWIGAT IHVTVTVCD 
      IKPISPGKI DNLTEVVII APEKKIHPI SNSNESKNT 
      DKTSKFLPE CKNNDENLI LRIVPFEEI VSQMRDLNS 
      PFNVFVLRA NVPQKIKDT INNTTLFLL KLLSNEDVD 
      KSLYVRLII VEDVLSDFV QNKLCLENV FVSNTLFTH 
      LNCEVGARI RLEPHISTL PEIKEIEIY TKRDYSIDV 
      VQKFQQYLA DNCKKHEFV LNSDVILEI GSNVRCLVR 
      FQPCQAKFC VVTQDLVRD CKLIFINET IPQREIFDK 
      KEFLVDEYA TDVANYNEI IDSCVFLCT YKTKSFNKL 
      ENVLITGKP GTGKTTLAT IIAHKINSY PFFIHTKKI 
      NCKSVKGKT VESLHKLLS TTFFDLIHH QPSVLILDN 
      LEILCENVN EGDALSPNT VYFNRVSEM LETLFEHFC 
      ENNAVAVIA TAESASKLH KNIYATRGH HLFKHVFGI 
      NQLNKNDRK KLIEFFFAN CNCDVGLEH LVEKTESFV 
      MQDLVDFVN KTLFESYQY DFDKDTMTI TEKCCETAL 
      ETVTETCLS GVNLLPSGD KNLDDIGGL SSVKKLLIE 
      TMLWPAKYP NLFSNAPLR LPSGLLLYG PPGTGKTIL 
      AGAAAKHCG LRLISIKGP ELLSKYIGA SEQAVRDVF 
      QRAQSAKPC ILFFDEFDS LAPRRGHDN TGVTDRVVN 
      QLLTQLDGI ETLSGVFVL AATSRPDLL DPALLRPGR 
      LDIHLHCPL PQENSRLEI LKVLSKCLN FSNDVDLGK 
      IASATEGFS GADLQAVLY SAQLDSVKE LLQDEANMQ 
      EFRCEIQQV QLMEAVKTT RPSLSKAEQ LKYERIYRH 
      FEGGGSSDF TPGSRATLA
    CDS sequence
    • >TC014648 RA
      atgtttgag ggattatta actgtcaga tacgtaacc 
      tcaaaaaac tgtttctgt gtcttgtct gttaataat 
      ttcaaacat ttacagaat aatgtcctc aaattgtcc 
      tacaatgac aatgaagca atccacgtg tccgttgac 
      cctttatca ggatcagtt gatgaccaa agtcttggt 
      attaattcg ttatatgcg aaggccttg ggccttgag 
      gacaattcg ttggttact gtttctgaa atatcaaaa 
      cctccaaca attcagagt gttacaata acggccgtt 
      gatagtaat gaccattta gttttggaa tcattagct 
      gaaaatgtt cagtccact ttgcttgag caaattcgt 
      gttctcaca aatggacaa aaatttgtc atttggatt 
      ggggcaaca attcatgta actgttact gtctgtgat 
      attaaacca atttctccc ggcaaaatt gataatctg 
      accgaagtc gtaataata gcaccagaa aaaaaaatt 
      catccaatt tcgaacagc aatgagtct aaaaacaca 
      gacaaaaca tcaaagttt ctgcctgaa tgtaaaaac 
      aatgatgaa aatttaatt ttgcgtatc gtgcctttt 
      gaagaaata gtttcgcaa atgcgcgat ttaaattct 
      ccatttaac gtttttgtg ttgagggcc aatgtccca 
      caaaaaata aaagacaca ataaataac accacattg 
      tttttacta aaattgttg agtaacgaa gacgtcgac 
      aaatcgctt tacgtcaga ttaattatt gtcgaggac 
      gttttaagc gattttgtc caaaataaa ttgtgctta 
      gagaatgta tttgtcagc aatacactt tttactcat 
      ttgaattgt gaagttggg gcacgaatt cgactagaa 
      ccccacatc agtactttg ccagaaatc aaagaaata 
      gaaatttac acaaaacgg gattattca attgatgtg 
      gttcaaaag tttcagcaa tacttagcc gacaattgc 
      aaaaaacac gaatttgtt ttaaattct gacgtaata 
      ttggaaatt ggcagtaat gtgagatgt ttggtacga 
      ttccaacct tgccaagca aaattttgt gtggtaaca 
      caagacttg gttcgagat tgcaaatta atttttatc 
      aacgaaaca attcctcag cgcgaaatt ttcgataaa 
      aaggaattt ttggtcgac gaatacgca acggatgtg 
      gctaactat aacgagatt atcgacagt tgtgtattt 
      ttgtgtacc tacaaaact aaaagtttt aataaattg 
      gaaaatgta ttaataaca gggaaacct ggtactggt 
      aaaacaact ttggctacg attattgca cataaaata 
      aactcttat ccctttttt attcataca aaaaaaatt 
      aattgcaag tcagtcaaa ggaaagacc gtcgaatcg 
      ttacataaa ctcttgtct acaactttc ttcgattta 
      attcaccat caaccctct gttttaatt ctcgataat 
      cttgaaatt ttgtgtgaa aacgttaac gaaggcgac 
      gccctgtcg ccaaatacg gtttatttc aacagagta 
      agtgaaatg ttggaaacg ctgtttgaa catttttgc 
      gaaaataac gccgtcgct gtaatagca acagctgaa 
      tcagcgtcc aaattacat aaaaatatt tatgcaacg 
      agaggccac caccttttt aaacacgtt tttggaatc 
      aatcagtta aataaaaat gataggaaa aaattgatc 
      gaatttttt ttcgctaat tgtaactgc gatgttggt 
      ttggagcac ttggttgaa aaaactgaa agtttcgtc 
      atgcaggac cttgttgat ttcgttaat aaaacttta 
      tttgagtcg taccaatac gattttgat aaagacaca 
      atgacaatc actgaaaag tgttgcgaa acggctttg 
      gaaacagta actgaaacg tgtttatca ggcgttaat 
      cttcttcca tctggtgat aaaaacttg gacgatatt 
      gggggttta tccagtgtc aaaaaactc ctaattgag 
      acaatgcta tggccggcg aaatatcct aatttgttt 
      tcaaacgcg ccccttcgt ctgccatcc ggtttgctc 
      ctttacggt cctcccggt acaggtaaa actatattg 
      gcaggagca gctgcaaaa cactgcgga cttcgctta 
      atttccata aagggaccc gaactttta tcgaaatat 
      atcggggct agcgagcaa gccgttcgg gatgtgttc 
      caaagagca caaagcgcc aagccatgc atattgttc 
      ttcgacgag ttcgacagc ctggctccc aggagaggc 
      cacgacaac acgggcgta acagaccgg gtcgtaaat 
      cagcttttg acccaactt gacggcatc gaaaccctt 
      tcaggggtg tttgtttta gcagcgact tcccgacct 
      gaccttcta gacccggct cttttacga cccggccgt 
      ctcgacatc caccttcat tgccctctt ccccaggaa 
      aattccaga ctcgagatt ttgaaagtt ctatcaaag 
      tgtttgaat ttttctaac gatgtggat ttaggaaaa 
      atcgccagc gctactgag ggtttttcc ggggctgac 
      ttacaggcc gtgctctac agcgcccag ctggactcc 
      gtcaaggag ctcctgcag gacgaggca aacatgcaa 
      gaattccgc tgcgagatt cagcaggtc cagctaatg 
      gaagccgta aaaaccacc aggccctct ttaagtaaa 
      gcggagcag ttgaaatac gagcgaata tacaggcac 
      ttcgagggc ggcggcagc agcgacttc acgccggga 
      agcagggcg acactagca tag
    mRNA sequence
    • >TC014648 RA
      actttcaaa aacgcgatt tgtaattta tggaaaagg 
      tttaattta tttatttaa gtgaaaagt gttgatttt 
      gcccagatg tataaattc ctctaattt ttttgcatt 
      tttttatgg tactcaact aaaaaatgt ttgagggat 
      tattaactg tcagatacg taacctcaa aaaactgtt 
      tctgtgtct tgtctgtta ataatttca aacatttac 
      agaataatg tcctcaaat tgtcctaca atgacaatg 
      aagcaatcc acgtgtccg ttgaccctt tatcaggat 
      cagttgatg accaaagtc ttggtatta attcgttat 
      atgcgaagg ccttgggcc ttgaggaca attcgttgg 
      ttactgttt ctgaaatat caaaacctc caacaattc 
      agagtgtta caataacgg ccgttgata gtaatgacc 
      atttagttt tggaatcat tagctgaaa atgttcagt 
      ccactttgc ttgagcaaa ttcgtgttc tcacaaatg 
      gacaaaaat ttgtcattt ggattgggg caacaattc 
      atgtaactg ttactgtct gtgatatta aaccaattt 
      ctcccggca aaattgata atctgaccg aagtcgtaa 
      taatagcac cagaaaaaa aaattcatc caatttcga 
      acagcaatg agtctaaaa acacagaca aaacatcaa 
      agtttctgc ctgaatgta aaaacaatg atgaaaatt 
      taattttgc gtatcgtgc cttttgaag aaatagttt 
      cgcaaatgc gcgatttaa attctccat ttaacgttt 
      ttgtgttga gggccaatg tcccacaaa aaataaaag 
      acacaataa ataacacca cattgtttt tactaaaat 
      tgttgagta acgaagacg tcgacaaat cgctttacg 
      tcagattaa ttattgtcg aggacgttt taagcgatt 
      ttgtccaaa ataaattgt gcttagaga atgtatttg 
      tcagcaata cacttttta ctcatttga attgtgaag 
      ttggggcac gaattcgac tagaacccc acatcagta 
      ctttgccag aaatcaaag aaatagaaa tttacacaa 
      aacgggatt attcaattg atgtggttc aaaagtttc 
      agcaatact tagccgaca attgcaaaa aacacgaat 
      ttgttttaa attctgacg taatattgg aaattggca 
      gtaatgtga gatgtttgg tacgattcc aaccttgcc 
      aagcaaaat tttgtgtgg taacacaag acttggttc 
      gagattgca aattaattt ttatcaacg aaacaattc 
      ctcagcgcg aaattttcg ataaaaagg aatttttgg 
      tcgacgaat acgcaacgg atgtggcta actataacg 
      agattatcg acagttgtg tatttttgt gtacctaca 
      aaactaaaa gttttaata aattggaaa atgtattaa 
      taacaggga aacctggta ctggtaaaa caactttgg 
      ctacgatta ttgcacata aaataaact cttatccct 
      tttttattc atacaaaaa aaattaatt gcaagtcag 
      tcaaaggaa agaccgtcg aatcgttac ataaactct 
      tgtctacaa ctttcttcg atttaattc accatcaac 
      cctctgttt taattctcg ataatcttg aaattttgt 
      gtgaaaacg ttaacgaag gcgacgccc tgtcgccaa 
      atacggttt atttcaaca gagtaagtg aaatgttgg 
      aaacgctgt ttgaacatt tttgcgaaa ataacgccg 
      tcgctgtaa tagcaacag ctgaatcag cgtccaaat 
      tacataaaa atatttatg caacgagag gccaccacc 
      tttttaaac acgtttttg gaatcaatc agttaaata 
      aaaatgata ggaaaaaat tgatcgaat tttttttcg 
      ctaattgta actgcgatg ttggtttgg agcacttgg 
      ttgaaaaaa ctgaaagtt tcgtcatgc aggaccttg 
      ttgatttcg ttaataaaa ctttatttg agtcgtacc 
      aatacgatt ttgataaag acacaatga caatcactg 
      aaaagtgtt gcgaaacgg ctttggaaa cagtaactg 
      aaacgtgtt tatcaggcg ttaatcttc ttccatctg 
      gtgataaaa acttggacg atattgggg gtttatcca 
      gtgtcaaaa aactcctaa ttgagacaa tgctatggc 
      cggcgaaat atcctaatt tgttttcaa acgcgcccc 
      ttcgtctgc catccggtt tgctccttt acggtcctc 
      ccggtacag gtaaaacta tattggcag gagcagctg 
      caaaacact gcggacttc gcttaattt ccataaagg 
      gacccgaac ttttatcga aatatatcg gggctagcg 
      agcaagccg ttcgggatg tgttccaaa gagcacaaa 
      gcgccaagc catgcatat tgttcttcg acgagttcg 
      acagcctgg ctcccagga gaggccacg acaacacgg 
      gcgtaacag accgggtcg taaatcagc ttttgaccc 
      aacttgacg gcatcgaaa ccctttcag gggtgtttg 
      ttttagcag cgacttccc gacctgacc ttctagacc 
      cggctcttt tacgacccg gccgtctcg acatccacc 
      ttcattgcc ctcttcccc aggaaaatt ccagactcg 
      agattttga aagttctat caaagtgtt tgaattttt 
      ctaacgatg tggatttag gaaaaatcg ccagcgcta 
      ctgagggtt tttccgggg ctgacttac aggccgtgc 
      tctacagcg cccagctgg actccgtca aggagctcc 
      tgcaggacg aggcaaaca tgcaagaat tccgctgcg 
      agattcagc aggtccagc taatggaag ccgtaaaaa 
      ccaccaggc cctctttaa gtaaagcgg agcagttga 
      aatacgagc gaatataca ggcacttcg agggcggcg 
      gcagcagcg acttcacgc cgggaagca gggcgacac 
      tagcatagt gaattgttt ttgggctgg ttttaataa 
      attattgat tttctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY