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Gene information
General information
TC013167 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12814778..12817615
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013167
    iBeetle screen iB_09121

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013167 RA
      MKVVSENIA MAPLKPLSR AVTGAIRTN QKASVSTRS 
      RSSVLTNAQ IRPKRKADF SPVKENVKR SAMGDITNN 
      KKFDDKKKA TLKQPAKKT IVQVKTLPS VRTVLKPKQ 
      NENVLPPQA PCLNGVVTR AAVKNVTTG QTAVKPKEA 
      LKEISNPRI KTRLSNEFE KTEESLYSS ALEELNDSV 
      SSSGAKAKT PPSDEKSPN LSVVSIVAQ QLQNKLNLG 
      IHEVPEGVP DFDKENWDD VHQVSHYAM DIFNYMKSR 
      EPQFEIADY MDRQVCLTS WMRSLLVDW MVEVQESFE 
      LNHETLYLG VKLVDFYLS RMTVGKETL QLVGAASMF 
      IASKYDERI PPAINDFLY ICDGAYTCR ELVRMEINI 
      LKVCDFNLG IPISYRFLR RYARCAKIT MPVLTLARF 
      ILEYSLMDY ATVTVRDSK LASASLYLA LKMKKISGW 
      TTALEFYSG YKLEDFKEV VILLNDGLH KKPKSPLMT 
      VRNKYSHKI FFEVAKIPL ISNEELFV
    CDS sequence
    • >TC013167 RA
      atgaaagtt gtgagtgag aacattgcg atggctccc 
      ttgaagccc ctaagcaga gccgtcaca ggcgccatc 
      cgtacaaac caaaaagcc tcagtttcc acccgaagc 
      cggtcatca gtcctgaca aacgcccaa atccgccca 
      aaacgaaaa gccgacttt tcccccgtc aaagaaaat 
      gttaaacga tcagcaatg ggtgacatc accaacaac 
      aaaaagttc gatgataaa aagaaagcc actttaaaa 
      cagcccgcg aagaagacg atcgttcaa gtcaaaaca 
      ctgccctcg gtgagaacc gtgctcaag cccaagcag 
      aacgagaat gttctgccc ccgcaagcc ccctgtctc 
      aacggggtg gtgactagg gctgctgtg aaaaacgtg 
      acgactggc cagacggca gtcaaaccc aaagaagcg 
      ctcaaagag atctctaat cctcgcatc aagacaaga 
      ctgagcaac gagtttgaa aagactgaa gaatctctc 
      tattcgtca gcgttggag gaactcaat gattccgtt 
      agttccagc ggagccaaa gcgaaaacg ccccccagt 
      gatgagaaa tccccgaac ctatctgtg gtgtccatc 
      gtggcccag cagctccag aacaaactc aatctgggc 
      attcatgaa gttcccgag ggagtgccc gactttgat 
      aaggagaac tgggacgac gtgcaccag gtgtcccac 
      tacgccatg gacattttc aattacatg aagagtcga 
      gagccgcag tttgaaatc gccgactac atggacaga 
      caagtctgt ctcacgagc tggatgcgg tcgcttctg 
      gtggactgg atggttgaa gtgcaagaa agtttcgaa 
      ctgaaccac gagacgctt tacttagga gtaaaatta 
      gtggatttt tacttaagt cggatgact gtcggcaaa 
      gagaccttg caattagtg ggcgcagct tccatgttc 
      atagctagt aaatacgac gaaagaatt ccgccggcc 
      atcaacgac tttttgtac atttgtgat ggggcatac 
      acgtgccgg gagctcgtc cgaatggaa ataaatatt 
      ttaaaagtc tgtgatttt aatctgggc attccaatc 
      agctacaga tttctcagg agatacgct agatgtgcc 
      aaaatcaca atgcctgtg ctcaccctc gcaagattt 
      attttagaa tattcgctg atggattat gccactgtt 
      actgtacgc gactcaaaa ttggcctca gcgtcactg 
      tatctggcg ctcaagatg aagaaaatt agtgggtgg 
      acgaccgcc ttagagttt tattcaggc tacaaactt 
      gaagatttt aaagaagtt gtgatttta ctaaacgac 
      gggttacac aaaaagccg aaaagtccg ctaatgact 
      gtaagaaat aagtattcc cataagata ttctttgaa 
      gtagccaag attccacta ataagtaac gaagaattg 
      tttgtttag
    mRNA sequence
    • >TC013167 RA
      ccttctatt ttatttaac taccaatgg ggacgaagc 
      agtgaaacg tttcaaaac aactgcaag ccgacaagc 
      tttaaacat caacgaact tcacatgtg aatttttac 
      aagcttttc tacactttt ctacaagca attttacaa 
      gttttaagg agggactca tcatcacac acatacaca 
      aataacccg agacatttc tgttaatta ataatgaaa 
      gttgtgagt gagaacatt gcgatggct cccttgaag 
      cccctaagc agagccgtc acaggcgcc atccgtaca 
      aaccaaaaa gcctcagtt tccacccga agccggtca 
      tcagtcctg acaaacgcc caaatccgc ccaaaacga 
      aaagccgac ttttccccc gtcaaagaa aatgttaaa 
      cgatcagca atgggtgac atcaccaac aacaaaaag 
      ttcgatgat aaaaagaaa gccacttta aaacagccc 
      gcgaagaag acgatcgtt caagtcaaa acactgccc 
      tcggtgaga accgtgctc aagcccaag cagaacgag 
      aatgttctg cccccgcaa gccccctgt ctcaacggg 
      gtggtgact agggctgct gtgaaaaac gtgacgact 
      ggccagacg gcagtcaaa cccaaagaa gcgctcaaa 
      gagatctct aatcctcgc atcaagaca agactgagc 
      aacgagttt gaaaagact gaagaatct ctctattcg 
      tcagcgttg gaggaactc aatgattcc gttagttcc 
      agcggagcc aaagcgaaa acgcccccc agtgatgag 
      aaatccccg aacctatct gtggtgtcc atcgtggcc 
      cagcagctc cagaacaaa ctcaatctg ggcattcat 
      gaagttccc gagggagtg cccgacttt gataaggag 
      aactgggac gacgtgcac caggtgtcc cactacgcc 
      atggacatt ttcaattac atgaagagt cgagagccg 
      cagtttgaa atcgccgac tacatggac agacaagtc 
      tgtctcacg agctggatg cggtcgctt ctggtggac 
      tggatggtt gaagtgcaa gaaagtttc gaactgaac 
      cacgagacg ctttactta ggagtaaaa ttagtggat 
      ttttactta agtcggatg actgtcggc aaagagacc 
      ttgcaatta gtgggcgca gcttccatg ttcatagct 
      agtaaatac gacgaaaga attccgccg gccatcaac 
      gactttttg tacatttgt gatggggca tacacgtgc 
      cgggagctc gtccgaatg gaaataaat attttaaaa 
      gtctgtgat tttaatctg ggcattcca atcagctac 
      agatttctc aggagatac gctagatgt gccaaaatc 
      acaatgcct gtgctcacc ctcgcaaga tttatttta 
      gaatattcg ctgatggat tatgccact gttactgta 
      cgcgactca aaattggcc tcagcgtca ctgtatctg 
      gcgctcaag atgaagaaa attagtggg tggacgacc 
      gccttagag ttttattca ggctacaaa cttgaagat 
      tttaaagaa gttgtgatt ttactaaac gacgggtta 
      cacaaaaag ccgaaaagt ccgctaatg actgtaaga 
      aataagtat tcccataag atattcttt gaagtagcc 
      aagattcca ctaataagt aacgaagaa ttgtttgtt 
      taggccaat tgatgggta aatgtctgt agtttttga 
      cgctttact tgttcgtaa agtgagttt ttatatctg 
      attattcgc attatttaa gtccaggtc tgtctagat 
      gtatttatt gttttaaaa ttatattaa tttttatcg 
      tgtgtggca ttaagtaaa ttttcgtca aattttatt 
      acggttgat gaagacatc gacaagata tggtttgta 
      cttgttgat ttatgttga ataatgagt aatttaagt 
      tacacaagc attgaatga ttattacct aacgtttga 
      actttaggc gccgttttt acgtaagaa gttacatca 
      ggagaattg ctcgttcaa ttgaataaa acatttcgt 
      gttat
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