Status
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Gene information
General information
TC014647 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12811927..12814771
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014647
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09120

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 3

    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014647 RA
      MGQKFQYDE SGSTFFYFL LSFLALILI PATIYYWPR 
      EKKRDPEEE SKKCHCPPC IKKREFQKN ADPWKGPKN 
      FLIKLVIII GWLLLLLLA YKVSQFDYE MANFDPYEI 
      LGIPLGASQ AEIKKAYRR LSLILHPDK DTGNEKEFM 
      KLSKAYQAL TDDEARKNW EKYGNPDGP GAMSFGIAL 
      PSWIVEKEN SVWVLGLYG LVFMVALPI VVGTWWYRS 
      IKFTDQVLL DTTQMYYYF FHKTPNMVL KRVIMILAA 
      SLEFNKKHN SEIKERDLD NEEVPQLIK KLPNLSEKN 
      KEAPLCYTY SIKARAIIH AHLSRIPLN PNTLEEDRR 
      YIIAKCPTL IQEQVNCVN QLIVLAYNR RIQRLPTIE 
      TIENCMKLC PMIVQGLWE FKSPLLQLP YINEDNLKY 
      FMNKKKPIR SLQQFAQLK GDERRNILR NFSEQEYDN 
      VMKVLGNMP YIDFQVKYE VMDDENPTE VTVGAIVTV 
      IVTLTRKPM STLFGDETV KDNNLINEN GVDGDAKEA 
      AAGDTDPQV PVIKRPAWL KQKRGGGKK NKKPVKQPK 
      ATAAVVKPK ADESPVPSS DKKKVKEEK SKEEESEES 
      DVSDAETND KSSEDDSVQ KSQNEDDDQ EWEKFKKLH 
      EREKALQGK SKTSHPVHS PYFPNDKQE YWWTYICDR 
      KSRTLLTVP HYVTSLIDQ EVVHLKFTA PNWVGVYTF 
      TVCLRSDSY IGFDQQKDI KLDVKAAPA EITEHPQWE 
      DLEEEEGSA KEDAHESEY TTDEDLSED DD
    CDS sequence
    • >TC014647 RA
      atggggcaa aaattccaa tatgatgaa agtggttcg 
      acgtttttc tacttctta ctatcattt ttagcctta 
      atcttaatc ccagcgacg atatactac tggccgaga 
      gaaaaaaag agagacccc gaggaagag agtaaaaaa 
      tgccactgc cccccatgt atcaagaaa agggagttc 
      caaaagaac gcggatcct tggaagggc cccaagaat 
      tttttaata aagctagtg ataataatt gggtggttg 
      ctactactg ctgttagcg tacaaagta tctcagttt 
      gattatgaa atggcaaac tttgaccct tatgaaata 
      ctggggatc ccccttggg gcgagccaa gccgagatc 
      aagaaagcc tacaggaga ctctctctc atccttcat 
      cctgacaaa gataccggc aatgagaaa gaattcatg 
      aagttgtcg aaagcctac caggcgtta actgacgat 
      gaagcgaga aaaaactgg gagaaatac ggaaatccg 
      gacggtccc ggagccatg agttttggg atagcgctc 
      ccgtcgtgg attgtggag aaggagaac agcgtgtgg 
      gtgctgggg ctgtatggg cttgtcttc atggtggcg 
      cttccaata gtggtcggg acgtggtgg tacagaagc 
      ataaaattc accgaccaa gtcttgcta gacacaacc 
      cagatgtac tactacttt ttccataaa actcccaac 
      atggtgctc aagcgtgta attatgatc ctagccgct 
      agtttagag tttaacaaa aaacacaat tcggagata 
      aaagagcga gatttggac aacgaggag gtgccccaa 
      ctcatcaaa aaattgcca aatctgagt gaaaagaat 
      aaagaagcg cccttgtgt tacacttat agtattaag 
      gctcgtgct atcattcat gctcacctg tcccgcata 
      cctctaaac cccaacact ttagaggaa gacaggcgt 
      tatatcatt gcaaaatgc ccaactcta atccaggaa 
      caggtcaac tgtgtcaat caactcata gtattggcg 
      tataatcgc agaatacaa agattacct acaattgag 
      acaatagaa aactgtatg aaactgtgc ccaatgatc 
      gtccaaggc ttgtgggaa tttaagtcg cctttactt 
      caactacct tacattaat gaagacaac ttgaagtat 
      tttatgaat aagaagaag cccatcagg tctctacaa 
      cagtttgcc caactaaaa ggggacgaa aggaggaat 
      attttgcga aatttcagc gagcaggag tatgacaat 
      gtgatgaaa gttttggga aacatgcct tacattgac 
      ttccaagtc aaatacgaa gttatggat gatgagaac 
      cccactgaa gtgaccgtt ggggcgatc gtgaccgtc 
      atagtgacc ctaacccgc aagccaatg agcaccttg 
      tttggtgac gaaactgtg aaagacaac aacctgatc 
      aacgaaaac ggcgttgac ggtgacgct aaagaagca 
      gctgctggt gacactgat cctcaagtc ccggtaata 
      aaacgtcca gcttggctg aagcaaaaa cgaggcggt 
      ggaaaaaag aacaaaaaa cctgtcaaa caaccgaaa 
      gccaccgcc gcagtggtt aaacccaaa gcggacgag 
      tctccagtc ccatcgtcc gacaagaag aaagtcaag 
      gaagagaaa agtaaagaa gaagaaagc gaagagtcg 
      gatgtgagc gacgctgaa acgaacgac aagtcgtca 
      gaggatgac agtgtccag aagtcgcag aatgaggat 
      gatgatcaa gagtgggag aagttcaag aagttgcat 
      gagagggag aaggcgttg caaggcaaa tcaaagact 
      tcgcatcca gtgcatagt ccttacttt cctaacgac 
      aagcaggag tattggtgg acgtatatt tgcgatagg 
      aagtcaagg acgctctta acagtgcct cattatgtc 
      accagttta atagaccag gaagttgta cacttaaaa 
      ttcacagct ccaaactgg gtaggagtt tacactttc 
      accgtgtgt ctgaggtcg gattcgtat atagggttt 
      gatcagcag aaagatatc aaattagat gtgaaagct 
      gcgcctgct gaaatcaca gagcacccg caatgggag 
      gatctggag gaggaggag gggtcggct aaggaggac 
      gcccacgag tcggagtat acaacagat gaagacctt 
      tcagaggat gacgactga
    mRNA sequence
    • >TC014647 RA
      gcgcaacaa tttgaagta ggtttcgaa tcgaattgt 
      caaatatta cacgtcgtc caattttaa taaataaga 
      ggaaatggg gcaaaaatt ccaatatga tgaaagtgg 
      ttcgacgtt tttctactt cttactatc atttttagc 
      cttaatctt aatcccagc gacgatata ctactggcc 
      gagagaaaa aaagagaga ccccgagga agagagtaa 
      aaaatgcca ctgcccccc atgtatcaa gaaaaggga 
      gttccaaaa gaacgcgga tccttggaa gggccccaa 
      gaatttttt aataaagct agtgataat aattgggtg 
      gttgctact actgctgtt agcgtacaa agtatctca 
      gtttgatta tgaaatggc aaactttga cccttatga 
      aatactggg gatccccct tggggcgag ccaagccga 
      gatcaagaa agcctacag gagactctc tctcatcct 
      tcatcctga caaagatac cggcaatga gaaagaatt 
      catgaagtt gtcgaaagc ctaccaggc gttaactga 
      cgatgaagc gagaaaaaa ctgggagaa atacggaaa 
      tccggacgg tcccggagc catgagttt tgggatagc 
      gctcccgtc gtggattgt ggagaagga gaacagcgt 
      gtgggtgct ggggctgta tgggcttgt cttcatggt 
      ggcgcttcc aatagtggt cgggacgtg gtggtacag 
      aagcataaa attcaccga ccaagtctt gctagacac 
      aacccagat gtactacta ctttttcca taaaactcc 
      caacatggt gctcaagcg tgtaattat gatcctagc 
      cgctagttt agagtttaa caaaaaaca caattcgga 
      gataaaaga gcgagattt ggacaacga ggaggtgcc 
      ccaactcat caaaaaatt gccaaatct gagtgaaaa 
      gaataaaga agcgccctt gtgttacac ttatagtat 
      taaggctcg tgctatcat tcatgctca cctgtcccg 
      catacctct aaaccccaa cactttaga ggaagacag 
      gcgttatat cattgcaaa atgcccaac tctaatcca 
      ggaacaggt caactgtgt caatcaact catagtatt 
      ggcgtataa tcgcagaat acaaagatt acctacaat 
      tgagacaat agaaaactg tatgaaact gtgcccaat 
      gatcgtcca aggcttgtg ggaatttaa gtcgccttt 
      acttcaact accttacat taatgaaga caacttgaa 
      gtattttat gaataagaa gaagcccat caggtctct 
      acaacagtt tgcccaact aaaagggga cgaaaggag 
      gaatatttt gcgaaattt cagcgagca ggagtatga 
      caatgtgat gaaagtttt gggaaacat gccttacat 
      tgacttcca agtcaaata cgaagttat ggatgatga 
      gaaccccac tgaagtgac cgttggggc gatcgtgac 
      cgtcatagt gaccctaac ccgcaagcc aatgagcac 
      cttgtttgg tgacgaaac tgtgaaaga caacaacct 
      gatcaacga aaacggcgt tgacggtga cgctaaaga 
      agcagctgc tggtgacac tgatcctca agtcccggt 
      aataaaacg tccagcttg gctgaagca aaaacgagg 
      cggtggaaa aaagaacaa aaaacctgt caaacaacc 
      gaaagccac cgccgcagt ggttaaacc caaagcgga 
      cgagtctcc agtcccatc gtccgacaa gaagaaagt 
      caaggaaga gaaaagtaa agaagaaga aagcgaaga 
      gtcggatgt gagcgacgc tgaaacgaa cgacaagtc 
      gtcagagga tgacagtgt ccagaagtc gcagaatga 
      ggatgatga tcaagagtg ggagaagtt caagaagtt 
      gcatgagag ggagaaggc gttgcaagg caaatcaaa 
      gacttcgca tccagtgca tagtcctta ctttcctaa 
      cgacaagca ggagtattg gtggacgta tatttgcga 
      taggaagtc aaggacgct cttaacagt gcctcatta 
      tgtcaccag tttaataga ccaggaagt tgtacactt 
      aaaattcac agctccaaa ctgggtagg agtttacac 
      tttcaccgt gtgtctgag gtcggattc gtatatagg 
      gtttgatca gcagaaaga tatcaaatt agatgtgaa 
      agctgcgcc tgctgaaat cacagagca cccgcaatg 
      ggaggatct ggaggagga ggaggggtc ggctaagga 
      ggacgccca cgagtcgga gtatacaac agatgaaga 
      cctttcaga ggatgacga ctgattttt acaatgtaa 
      tcaaagaaa gacttagag atagcattt atgtagtaa 
      aatattaat tctggtcat ctctcttta actggattc 
      gacattatt ttcaatgtt aaggttttg ggctgtatt 
      tatatttgt accataaag tgaaatatt tactttgtt 
      ttcgtccat acatccccg aactacaaa taaatctaa 
      cgtaattaa gctaatgaa aggatgcaa ggtgaagaa 
      aaaccattg acattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY