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Gene information
General information
TC013181 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12650647..12652915
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013181
    iBeetle screen iB_09117

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013181 RA
      MAGNTHLSP EQKYELITR NLQEVLGES KIKTILSQR 
      DLKIYWGTA TTGKPHIAY FVPMSKIAD FLRAGVEVT 
      ILFADLHAY LDNMKAPWE LLALRVQYY EHTIKAMLK 
      SIGVPLEKL RFEKGTNYQ LSKEYTLDV YRLSSIITE 
      HDAKKAGAE VVKQVDHPL VSGLLYPGL QALDEEYLK 
      VDAQFGGVD QRKIFTFAE KYLPQLGYQ KRAHLMNPM 
      VPGLTGVKM SSSEEDSKI DLLDSPAAV KQKLKKAFC 
      EPGNIENNG ILSFAKHVI FPLLKPEEK FVVPRKADY 
      GGDINFESF KQLETAFAN QEVHPGDLK ASIEIYLNR 
      LLDPVRKTF EDPELKKLA ERAYPVVKK VDDEIKPHV 
      LDIRVGKIV EISKHPDAD SLYVSKIEV GEDAPRTVI 
      SGLVNYYSA EELLNRMVV LLCNLKPSK MRGIESTGM 
      VLCASTDEP KKVETLIPP ESCSPGDKL VFENYEDGT 
      PEPVLNPKK KVWEKLQAD FRTNSDCIA QWKSSNLIS 
      KAGDVVRSK TLADTLIK
    CDS sequence
    • >TC013181 RA
      atggctggt aatacacac ctctcgcct gagcaaaaa 
      tacgagtta ataactcgt aatttacaa gaagtcctg 
      ggcgaaagt aaaataaaa acgatttta tcgcaacgt 
      gacttaaaa atctactgg ggaacggca acaacgggt 
      aaaccccac atcgcctat ttcgtcccc atgtcgaaa 
      atcgccgac tttttgcgc gccggtgtc gaagtcacc 
      attcttttt gccgatctg catgcttat ctagacaac 
      atgaaggca ccctgggag ctgttagcc ctccgggtg 
      caatattac gaacacacg attaaagcg atgctgaaa 
      tcgattgga gttccctta gaaaagctg cggttcgaa 
      aaagggacc aactaccaa ctgtccaag gaatacacc 
      ttggacgtg taccgcctg agctcgatt ataaccgaa 
      cacgatgcc aaaaaagcc ggcgctgaa gtggtcaaa 
      caagtggat caccccttg gtcagcgga ctcttgtac 
      cccgggttg caagccctg gatgaagag tatctgaaa 
      gtggacgca cagtttggt ggggttgac cagaggaag 
      atcttcact tttgccgaa aaatacctc ccgcagttg 
      gggtaccag aagcgggca catctgatg aacccaatg 
      gttccaggt ttaacgggc gttaaaatg tcgtcttcg 
      gaagaagac agcaagatc gatttgctc gattctccc 
      gcagctgtg aaacagaaa ctgaaaaaa gcattttgc 
      gaacctgga aatatcgaa aataacggg attttatcg 
      tttgccaaa cacgtaata tttccactc ttgaagcca 
      gaggaaaaa ttcgtggtt ccgcgtaaa gctgactac 
      ggtggcgat attaatttc gaatcgttt aaacaactg 
      gaaacggca tttgccaat caagaagtc caccctggt 
      gatctcaaa gccagtatt gagatttat ctaaatcgg 
      ttattggac cctgtaagg aagactttt gaagacccc 
      gaattgaaa aaactggca gaaagagcc tatcctgtt 
      gtgaagaaa gtggacgat gagattaaa ccacacgtt 
      ctggatatc agagtgggg aaaattgtg gaaatttcc 
      aaacatcca gacgctgat tctctgtat gttagcaag 
      attgaggtg ggagaggac gctccaagg actgtgata 
      agtggtctt gttaattac tattcagcc gaagaattg 
      ctaaatcgc atggttgtg ctgctttgt aatttaaaa 
      ccgtcgaaa atgagaggg attgagtcc acaggaatg 
      gttttgtgt gcttcaaca gatgagccg aaaaaagtt 
      gaaacactt atcccacct gaaagttgc agtcctggg 
      gataagcta gtttttgaa aattacgaa gatggaacg 
      cctgaaccc gtccttaac ccgaaaaag aaagtttgg 
      gagaaattg caagccgat tttcggact aattccgac 
      tgtatcgcg caatggaaa agtagtaat ttgatttca 
      aaggcgggt gatgtagta cggagtaaa acactagcc 
      gatacttta atcaaatag
    mRNA sequence
    • >TC013181 RA
      gcacatgtt aaagcagaa ttacgaaga attctcttc 
      gattttgtg taaggaaat tatctcttg tgatcccac 
      agtgtctta tactacttc cataaaaaa attgatttt 
      ttcacacca tggctggta atacacacc tctcgcctg 
      agcaaaaat acgagttaa taactcgta atttacaag 
      aagtcctgg gcgaaagta aaataaaaa cgattttat 
      cgcaacgtg acttaaaaa tctactggg gaacggcaa 
      caacgggta aaccccaca tcgcctatt tcgtcccca 
      tgtcgaaaa tcgccgact ttttgcgcg ccggtgtcg 
      aagtcacca ttctttttg ccgatctgc atgcttatc 
      tagacaaca tgaaggcac cctgggagc tgttagccc 
      tccgggtgc aatattacg aacacacga ttaaagcga 
      tgctgaaat cgattggag ttcccttag aaaagctgc 
      ggttcgaaa aagggacca actaccaac tgtccaagg 
      aatacacct tggacgtgt accgcctga gctcgatta 
      taaccgaac acgatgcca aaaaagccg gcgctgaag 
      tggtcaaac aagtggatc accccttgg tcagcggac 
      tcttgtacc ccgggttgc aagccctgg atgaagagt 
      atctgaaag tggacgcac agtttggtg gggttgacc 
      agaggaaga tcttcactt ttgccgaaa aatacctcc 
      cgcagttgg ggtaccaga agcgggcac atctgatga 
      acccaatgg ttccaggtt taacgggcg ttaaaatgt 
      cgtcttcgg aagaagaca gcaagatcg atttgctcg 
      attctcccg cagctgtga aacagaaac tgaaaaaag 
      cattttgcg aacctggaa atatcgaaa ataacggga 
      ttttatcgt ttgccaaac acgtaatat ttccactct 
      tgaagccag aggaaaaat tcgtggttc cgcgtaaag 
      ctgactacg gtggcgata ttaatttcg aatcgttta 
      aacaactgg aaacggcat ttgccaatc aagaagtcc 
      accctggtg atctcaaag ccagtattg agatttatc 
      taaatcggt tattggacc ctgtaagga agacttttg 
      aagaccccg aattgaaaa aactggcag aaagagcct 
      atcctgttg tgaagaaag tggacgatg agattaaac 
      cacacgttc tggatatca gagtgggga aaattgtgg 
      aaatttcca aacatccag acgctgatt ctctgtatg 
      ttagcaaga ttgaggtgg gagaggacg ctccaagga 
      ctgtgataa gtggtcttg ttaattact attcagccg 
      aagaattgc taaatcgca tggttgtgc tgctttgta 
      atttaaaac cgtcgaaaa tgagaggga ttgagtcca 
      caggaatgg ttttgtgtg cttcaacag atgagccga 
      aaaaagttg aaacactta tcccacctg aaagttgca 
      gtcctgggg ataagctag tttttgaaa attacgaag 
      atggaacgc ctgaacccg tccttaacc cgaaaaaga 
      aagtttggg agaaattgc aagccgatt ttcggacta 
      attccgact gtatcgcgc aatggaaaa gtagtaatt 
      tgatttcaa aggcgggtg atgtagtac ggagtaaaa 
      cactagccg atactttaa tcaaatagc attttttaa 
      tttgttgta cgcaatttt ttattttat aagttacga 
      agtgacaac tgcctctca aaatacaat gagcgaaca 
      aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY